#METABOLOMICS WORKBENCH engelmab_20231008_001358 DATATRACK_ID:4381 STUDY_ID:ST002919 ANALYSIS_ID:AN004789 PROJECT_ID:PR001814 VERSION 1 CREATED_ON October 10, 2023, 2:15 am #PROJECT PR:PROJECT_TITLE MINCH causes metabolic rewiring towards lipid accumulation and adipogenesis PR:PROJECT_SUMMARY Humans are ubiquitously exposed to plastic additives, including plasticizers. PR:PROJECT_SUMMARY There is growing evidence that exposure to certain plasticizers is associated PR:PROJECT_SUMMARY with the development of obesity due to their metabolism-disrupting properties. PR:PROJECT_SUMMARY Following the restriction of the use of the phthalate plasticizer PR:PROJECT_SUMMARY di-(2-ethylhexyl) phthalate (DEHP) due to its adverse health effects, it has PR:PROJECT_SUMMARY been replaced by new substitutes such as the plasticizer PR:PROJECT_SUMMARY diisononylcyclohexane-1,2-dicarboxylate (DINCH). Despite recent studies PR:PROJECT_SUMMARY suggesting that the primary metabolite monoisononylcyclohexane-1,2-dicarboxylic PR:PROJECT_SUMMARY acid ester (MINCH) promotes human adipocyte differentiation, the adipogenic PR:PROJECT_SUMMARY properties of MINCH remain controversial. Because the metabolome largely PR:PROJECT_SUMMARY reflects the molecular phenotype and is sensitive to perturbation by external PR:PROJECT_SUMMARY factors, we used targeted metabolomics to investigate the effects of DINCH and PR:PROJECT_SUMMARY MINCH on key metabolic pathways of adipocytes. Analysis of central carbon PR:PROJECT_SUMMARY metabolism is particularly relevant because it provides cellular energy through PR:PROJECT_SUMMARY the degradation of organic compounds and metabolic precursors for anabolic PR:PROJECT_SUMMARY functions that are critical for adipocyte function, such as de novo lipogenesis. PR:PROJECT_SUMMARY The project consists of three main studies: analysis of the effects of DINCH and PR:PROJECT_SUMMARY MINCH on central carbon metabolism of human SGSB cells, analysis of the insulin PR:PROJECT_SUMMARY response of DINCH- and MINCH-treated SGSB cells, and analysis of the effects of PR:PROJECT_SUMMARY DINCH and MINCH on central carbon metabolism of human SGSB cells in the presence PR:PROJECT_SUMMARY of the PPARG inhibitor GW9662. PR:INSTITUTE Helmholtz Centre for Environmental Research PR:LAST_NAME Engelmann PR:FIRST_NAME Beatrice PR:ADDRESS Permoserstr. 15 PR:EMAIL beatrice.engelmann@ufz.de PR:PHONE 00493412351099 #STUDY ST:STUDY_TITLE Short-term metabolic insulin response of DINCH- and MINCH-treated cells ST:STUDY_SUMMARY In the second part of the project, we examined the short-term metabolic insulin ST:STUDY_SUMMARY response of DINAH- and MINCH-treated cells and compared them with ST:STUDY_SUMMARY rosiglitazone-differentiated cells. For this purpose, the human SGBS ST:STUDY_SUMMARY preadipocyte cell line was exposed to differentiation media conditioned with ST:STUDY_SUMMARY DINCH (10 nM or 10 µM), MINCH (10 nM or 10 µM), or rosiglitazone, and the ST:STUDY_SUMMARY insulin response was measured by analyzing the changes in glycolysis and PPP ST:STUDY_SUMMARY between insulin-stimulated and non-insulin-stimulated cells. In conclusion, the ST:STUDY_SUMMARY insulin response of glycolysis and PPP of cells treated with 10 µM MINCH, but ST:STUDY_SUMMARY not with 10 nM MINCH or DINCH, was similar to cells differentiated with ST:STUDY_SUMMARY rosiglitazone. ST:INSTITUTE Helmholtz Centre for Environmental Research ST:LAST_NAME Engelmann ST:FIRST_NAME Beatrice ST:ADDRESS Permoserstr. 15 ST:EMAIL beatrice.engelmann@ufz.de ST:PHONE 00493412351099 #SUBJECT SU:SUBJECT_TYPE Cultured cells SU:SUBJECT_SPECIES Homo sapiens SU:TAXONOMY_ID 9606 SU:GENDER Male #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data SUBJECT_SAMPLE_FACTORS - DINCH_High_-_1 Treatment:DINCH 10µM minus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_-_2 Treatment:DINCH 10µM minus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_-_3 Treatment:DINCH 10µM minus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_-_4 Treatment:DINCH 10µM minus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_-_5 Treatment:DINCH 10µM minus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_+_1 Treatment:DINCH 10µM plus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_+_2 Treatment:DINCH 10µM plus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_+_3 Treatment:DINCH 10µM plus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_+_4 Treatment:DINCH 10µM plus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_+_5 Treatment:DINCH 10µM plus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_-_1 Treatment:DINCH 10nM minus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_-_2 Treatment:DINCH 10nM minus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_-_3 Treatment:DINCH 10nM minus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_-_4 Treatment:DINCH 10nM minus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_-_5 Treatment:DINCH 10nM minus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_+_1 Treatment:DINCH 10nM plus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_+_2 Treatment:DINCH 10nM plus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_+_3 Treatment:DINCH 10nM plus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_+_4 Treatment:DINCH 10nM plus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_+_5 Treatment:DINCH 10nM plus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_-_1 Treatment:DINCH 10µM minus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_-_2 Treatment:DINCH 10µM minus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_-_3 Treatment:DINCH 10µM minus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_-_4 Treatment:DINCH 10µM minus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_-_5 Treatment:DINCH 10µM minus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_+_1 Treatment:DINCH 10µM plus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_+_2 Treatment:DINCH 10µM plus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_+_3 Treatment:DINCH 10µM plus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_+_4 Treatment:DINCH 10µM plus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_+_5 Treatment:DINCH 10µM plus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_-_1 Treatment:DINCH 10nM minus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_-_2 Treatment:DINCH 10nM minus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_-_3 Treatment:DINCH 10nM minus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_-_4 Treatment:DINCH 10nM minus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_-_5 Treatment:DINCH 10nM minus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_+_1 Treatment:DINCH 10nM plus Insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_+_2 Treatment:DINCH 10nM plus Insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_+_3 Treatment:DINCH 10nM plus Insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_+_4 Treatment:DINCH 10nM plus Insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_+_5 Treatment:DINCH 10nM plus Insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_-_1 Treatment:Rosiglitazone minus insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_-_2 Treatment:Rosiglitazone minus insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_-_3 Treatment:Rosiglitazone minus insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_-_4 Treatment:Rosiglitazone minus insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_-_5 Treatment:Rosiglitazone minus insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_+_1 Treatment:Rosiglitazone plus insulin | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_+_2 Treatment:Rosiglitazone plus insulin | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_+_3 Treatment:Rosiglitazone plus insulin | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_+_4 Treatment:Rosiglitazone plus insulin | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_+_5 Treatment:Rosiglitazone plus insulin | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220218_CG_intracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_-_1 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_-_2 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_-_3 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_-_4 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_-_5 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_+_1 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_+_2 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_+_3 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_+_4 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_High_SN_+_5 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_-_1 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_-_2 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_-_3 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_-_4 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_-_5 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_+_1 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_+_2 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_+_3 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_+_4 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - DINCH_Low_SN_+_5 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_-_1 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_-_2 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_-_3 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_-_4 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_-_5 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_+_1 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_+_2 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_+_3 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_+_4 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_High_SN_+_5 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_-_1 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_-_2 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_-_3 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_-_4 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_-_5 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_+_1 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_+_2 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_+_3 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_+_4 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - MINCH_Low_SN_+_5 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_-_1 Treatment:Rosiglitazone minus insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_-_2 Treatment:Rosiglitazone minus insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_-_3 Treatment:Rosiglitazone minus insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_-_4 Treatment:Rosiglitazone minus insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_-_5 Treatment:Rosiglitazone minus insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_+_1 Treatment:Rosiglitazone plus insulin supernatant | Replicate:1 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_+_2 Treatment:Rosiglitazone plus insulin supernatant | Replicate:2 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_+_3 Treatment:Rosiglitazone plus insulin supernatant | Replicate:3 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_+_4 Treatment:Rosiglitazone plus insulin supernatant | Replicate:4 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff SUBJECT_SAMPLE_FACTORS - Rosi_SN_+_5 Treatment:Rosiglitazone plus insulin supernatant | Replicate:5 Species=Homo sapiens; RAW_FILE_NAME=Data20220221_CG_extracell_Insulinsens.wiff #COLLECTION CO:COLLECTION_SUMMARY The SGBS cells were obtained from Prof. Martin Wabitsch laboratory at the CO:COLLECTION_SUMMARY University Clinic Ulm. SGBS preadipocytes were differentiated according to the CO:COLLECTION_SUMMARY standard protocol described previously (Wabitsch et al., 2001). CO:SAMPLE_TYPE Adipose tissue CO:STORAGE_CONDITIONS -80℃ #TREATMENT TR:TREATMENT_SUMMARY SGSB preadipocytes were maintained at 37°C and 5% CO2 in 95% humidity. To TR:TREATMENT_SUMMARY examine the insulin response of SGBS treated with DINCH and MINCH, SGBS TR:TREATMENT_SUMMARY preadipocytes were treated for 18 days with differentiation media without the TR:TREATMENT_SUMMARY PPARG agonist rosiglitazone supplemented with DINCH or MINCH (10 nM and 10 µM). TR:TREATMENT_SUMMARY To obtain an adipogenesis reference, SGBS cells were differentiated with TR:TREATMENT_SUMMARY rosiglitazone for 18 days according to the standard differentiation protocol. TR:TREATMENT_SUMMARY After replacing the medium on day 17 with differentiation medium without insulin TR:TREATMENT_SUMMARY and overnight incubation (insulin starvation), cells were starved for 1 hour TR:TREATMENT_SUMMARY with differentiation medium without glucose and insulin (glucose + insulin TR:TREATMENT_SUMMARY starvation). Subsequently, cells were incubated with either differentiation TR:TREATMENT_SUMMARY medium with insulin (insulin-stimulated cells correspond to plus-insulin TR:TREATMENT_SUMMARY samples) or without insulin (unstimulated cells correspond to minus-insulin TR:TREATMENT_SUMMARY samples). During differentiation, a final concentration of 0.01% (v/v) MeOH and TR:TREATMENT_SUMMARY 0.02% (v/v) DMSO was added to all differentiation media. Continuous exposure was TR:TREATMENT_SUMMARY mimicked by replacing the cell culture medium every other day. Each treatment TR:TREATMENT_SUMMARY was performed in five biological replicates (n=5). #SAMPLEPREP SP:SAMPLEPREP_SUMMARY Extraction of intracellular and extracellular metabolites was performed by a SP:SAMPLEPREP_SUMMARY 1:1:1 methanol:water:chloroform extraction protocol. For the extraction of SP:SAMPLEPREP_SUMMARY intracellular metabolites, the culture medium was removed and the cells were SP:SAMPLEPREP_SUMMARY rinsed twice with 1 ml of 0.9% ice-cold NaCl. The rinsing solution was removed, SP:SAMPLEPREP_SUMMARY and the metabolism of the cells was stopped by adding 400 µL of MeOH (-20 °C) SP:SAMPLEPREP_SUMMARY followed by 400 µL of ice-cold H2O containing 10 µM d6-glutarate. Cells were SP:SAMPLEPREP_SUMMARY collected using a cell lifter and 400 µL of chloroform was added. After shaking SP:SAMPLEPREP_SUMMARY at 1,400 rpm and 4 °C for 20 min, the extraction mixture was centrifuged at SP:SAMPLEPREP_SUMMARY 18,000 g and 4 °C for 5 min. Subsequently, 300 µL volume of the polar upper SP:SAMPLEPREP_SUMMARY phase was collected and evaporated to complete dryness. For the extraction of SP:SAMPLEPREP_SUMMARY extracellular metabolites, 300 µL of the supernatant was extracted by adding SP:SAMPLEPREP_SUMMARY 400 µL MeOH (-20 °C) containing 100 nM MEHP, 100 µL ice-cold H2O containing SP:SAMPLEPREP_SUMMARY 40 µM d6-glutarate, and 400 µL chloroform (-20 °C). Subsequent sample SP:SAMPLEPREP_SUMMARY preparation was identical to the extraction of intracellular metabolites. Note: SP:SAMPLEPREP_SUMMARY After measurement of the samples by LC-MS, the raw AUC values uploaded here were SP:SAMPLEPREP_SUMMARY normalized to the internal standard (d6-glutarate, if applicable) and DNA SP:SAMPLEPREP_SUMMARY content per well (measured by DAPI fluorescence). After normalization, log2 fold SP:SAMPLEPREP_SUMMARY changes were calculated by dividing the normalized peak area from each replicate SP:SAMPLEPREP_SUMMARY of each treatment by the normalized peak area from each control. Insulin data SP:SAMPLEPREP_SUMMARY were not normalized to DAPI because fold changes were calculated by dividing the SP:SAMPLEPREP_SUMMARY intensities of the insulin-stimulated cells by the noninsulin-stimulated cells SP:SAMPLEPREP_SUMMARY from each treatment. #CHROMATOGRAPHY CH:CHROMATOGRAPHY_TYPE Reversed phase CH:INSTRUMENT_NAME Agilent 1290 Infinity II CH:COLUMN_NAME Waters Xselect XP HSS T3 (150 x 2.1mm, 2.5µm) CH:SOLVENT_A 10mM TBA, 10mM acetic acid, 5% MeOH, 2% IPA in water CH:SOLVENT_B 100% IPA CH:FLOW_GRADIENT 0-5 min 0% B, 5-9 min 0%- 2% B, 9-9.5 min 2-6% B, 9.5-11.5 min 6% B, 11.5-12 min CH:FLOW_GRADIENT 6-11% B, 12-13.5 min 11% B, 13.5-15.5 min 11-28% B, 15.5-16.5 min 28-53% B, CH:FLOW_GRADIENT 16.5-22.5 53% B, 22.5-23 min 53-0% B, 23-33 min 0% B CH:FLOW_RATE 0-15.5 min 0.4 mL/min, 15.5-16.5 min 0.4-0.15 mL/min, 16.5-23 min 0.15 mL/min, CH:FLOW_RATE 23-27 min 0.15-0.4 mL/min, 27-33 min 0.4 mL/min CH:COLUMN_TEMPERATURE 40 #ANALYSIS AN:ANALYSIS_TYPE MS #MS MS:INSTRUMENT_NAME ABI Sciex 6500+ MS:INSTRUMENT_TYPE QTRAP MS:MS_TYPE ESI MS:ION_MODE NEGATIVE MS:MS_COMMENTS For identification and quantitation, a scheduled MRM method was used, with MS:MS_COMMENTS specific transitions for every metabolite. Data acquisition and peak integration MS:MS_COMMENTS were performed in Analyst® software (Version 1.7.1). #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak AUC MS_METABOLITE_DATA_START Samples DINCH_High_-_1 DINCH_High_-_2 DINCH_High_-_3 DINCH_High_-_4 DINCH_High_-_5 DINCH_High_+_1 DINCH_High_+_2 DINCH_High_+_3 DINCH_High_+_4 DINCH_High_+_5 DINCH_Low_-_1 DINCH_Low_-_2 DINCH_Low_-_3 DINCH_Low_-_4 DINCH_Low_-_5 DINCH_Low_+_1 DINCH_Low_+_2 DINCH_Low_+_3 DINCH_Low_+_4 DINCH_Low_+_5 MINCH_High_-_1 MINCH_High_-_2 MINCH_High_-_3 MINCH_High_-_4 MINCH_High_-_5 MINCH_High_+_1 MINCH_High_+_2 MINCH_High_+_3 MINCH_High_+_4 MINCH_High_+_5 MINCH_Low_-_1 MINCH_Low_-_2 MINCH_Low_-_3 MINCH_Low_-_4 MINCH_Low_-_5 MINCH_Low_+_1 MINCH_Low_+_2 MINCH_Low_+_3 MINCH_Low_+_4 MINCH_Low_+_5 Rosi_-_1 Rosi_-_2 Rosi_-_3 Rosi_-_4 Rosi_-_5 Rosi_+_1 Rosi_+_2 Rosi_+_3 Rosi_+_4 Rosi_+_5 DINCH_High_SN_-_1 DINCH_High_SN_-_2 DINCH_High_SN_-_3 DINCH_High_SN_-_4 DINCH_High_SN_-_5 DINCH_High_SN_+_1 DINCH_High_SN_+_2 DINCH_High_SN_+_3 DINCH_High_SN_+_4 DINCH_High_SN_+_5 DINCH_Low_SN_-_1 DINCH_Low_SN_-_2 DINCH_Low_SN_-_3 DINCH_Low_SN_-_4 DINCH_Low_SN_-_5 DINCH_Low_SN_+_1 DINCH_Low_SN_+_2 DINCH_Low_SN_+_3 DINCH_Low_SN_+_4 DINCH_Low_SN_+_5 MINCH_High_SN_-_1 MINCH_High_SN_-_2 MINCH_High_SN_-_3 MINCH_High_SN_-_4 MINCH_High_SN_-_5 MINCH_High_SN_+_1 MINCH_High_SN_+_2 MINCH_High_SN_+_3 MINCH_High_SN_+_4 MINCH_High_SN_+_5 MINCH_Low_SN_-_1 MINCH_Low_SN_-_2 MINCH_Low_SN_-_3 MINCH_Low_SN_-_4 MINCH_Low_SN_-_5 MINCH_Low_SN_+_1 MINCH_Low_SN_+_2 MINCH_Low_SN_+_3 MINCH_Low_SN_+_4 MINCH_Low_SN_+_5 Rosi_SN_-_1 Rosi_SN_-_2 Rosi_SN_-_3 Rosi_SN_-_4 Rosi_SN_-_5 Rosi_SN_+_1 Rosi_SN_+_2 Rosi_SN_+_3 Rosi_SN_+_4 Rosi_SN_+_5 Factors Treatment:DINCH 10µM minus Insulin | Replicate:1 Treatment:DINCH 10µM minus Insulin | Replicate:2 Treatment:DINCH 10µM minus Insulin | Replicate:3 Treatment:DINCH 10µM minus Insulin | Replicate:4 Treatment:DINCH 10µM minus Insulin | Replicate:5 Treatment:DINCH 10µM plus Insulin | Replicate:1 Treatment:DINCH 10µM plus Insulin | Replicate:2 Treatment:DINCH 10µM plus Insulin | Replicate:3 Treatment:DINCH 10µM plus Insulin | Replicate:4 Treatment:DINCH 10µM plus Insulin | Replicate:5 Treatment:DINCH 10nM minus Insulin | Replicate:1 Treatment:DINCH 10nM minus Insulin | Replicate:2 Treatment:DINCH 10nM minus Insulin | Replicate:3 Treatment:DINCH 10nM minus Insulin | Replicate:4 Treatment:DINCH 10nM minus Insulin | Replicate:5 Treatment:DINCH 10nM plus Insulin | Replicate:1 Treatment:DINCH 10nM plus Insulin | Replicate:2 Treatment:DINCH 10nM plus Insulin | Replicate:3 Treatment:DINCH 10nM plus Insulin | Replicate:4 Treatment:DINCH 10nM plus Insulin | Replicate:5 Treatment:DINCH 10µM minus Insulin | Replicate:1 Treatment:DINCH 10µM minus Insulin | Replicate:2 Treatment:DINCH 10µM minus Insulin | Replicate:3 Treatment:DINCH 10µM minus Insulin | Replicate:4 Treatment:DINCH 10µM minus Insulin | Replicate:5 Treatment:DINCH 10µM plus Insulin | Replicate:1 Treatment:DINCH 10µM plus Insulin | Replicate:2 Treatment:DINCH 10µM plus Insulin | Replicate:3 Treatment:DINCH 10µM plus Insulin | Replicate:4 Treatment:DINCH 10µM plus Insulin | Replicate:5 Treatment:DINCH 10nM minus Insulin | Replicate:1 Treatment:DINCH 10nM minus Insulin | Replicate:2 Treatment:DINCH 10nM minus Insulin | Replicate:3 Treatment:DINCH 10nM minus Insulin | Replicate:4 Treatment:DINCH 10nM minus Insulin | Replicate:5 Treatment:DINCH 10nM plus Insulin | Replicate:1 Treatment:DINCH 10nM plus Insulin | Replicate:2 Treatment:DINCH 10nM plus Insulin | Replicate:3 Treatment:DINCH 10nM plus Insulin | Replicate:4 Treatment:DINCH 10nM plus Insulin | Replicate:5 Treatment:Rosiglitazone minus insulin | Replicate:1 Treatment:Rosiglitazone minus insulin | Replicate:2 Treatment:Rosiglitazone minus insulin | Replicate:3 Treatment:Rosiglitazone minus insulin | Replicate:4 Treatment:Rosiglitazone minus insulin | Replicate:5 Treatment:Rosiglitazone plus insulin | Replicate:1 Treatment:Rosiglitazone plus insulin | Replicate:2 Treatment:Rosiglitazone plus insulin | Replicate:3 Treatment:Rosiglitazone plus insulin | Replicate:4 Treatment:Rosiglitazone plus insulin | Replicate:5 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:1 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:2 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:3 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:4 Treatment:DINCH 10µM minus Insulin supernatant | Replicate:5 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:1 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:2 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:3 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:4 Treatment:DINCH 10µM plus Insulin supernatant | Replicate:5 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:1 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:2 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:3 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:4 Treatment:DINCH 10nM minus Insulin supernatant | Replicate:5 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:1 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:2 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:3 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:4 Treatment:DINCH 10nM plus Insulin supernatant | Replicate:5 Treatment:Rosiglitazone minus insulin supernatant | Replicate:1 Treatment:Rosiglitazone minus insulin supernatant | Replicate:2 Treatment:Rosiglitazone minus insulin supernatant | Replicate:3 Treatment:Rosiglitazone minus insulin supernatant | Replicate:4 Treatment:Rosiglitazone minus insulin supernatant | Replicate:5 Treatment:Rosiglitazone plus insulin supernatant | Replicate:1 Treatment:Rosiglitazone plus insulin supernatant | Replicate:2 Treatment:Rosiglitazone plus insulin supernatant | Replicate:3 Treatment:Rosiglitazone plus insulin supernatant | Replicate:4 Treatment:Rosiglitazone plus insulin supernatant | Replicate:5 Glucose 6-phosphate 4.10E+03 2.44E+03 2.35E+03 3.52E+03 3.85E+03 5.45E+03 5.64E+03 6.70E+03 5.64E+03 4.28E+03 2.03E+03 1.57E+03 9.08E+02 1.93E+03 2.01E+03 1.33E+03 1.58E+03 9.11E+02 1.28E+04 1.34E+04 1.14E+04 1.04E+04 8.19E+03 1.13E+05 1.09E+05 8.74E+04 8.77E+04 8.22E+04 1.51E+03 1.85E+03 7.95E+02 2.12E+03 1.93E+03 1.11E+03 2.31E+03 1.80E+03 1.82E+04 1.23E+04 1.24E+04 1.22E+04 1.03E+04 1.06E+05 1.20E+05 1.21E+05 1.20E+05 1.33E+05 Lactate 1.01E+06 1.22E+06 1.89E+06 6.44E+06 1.64E+07 1.82E+06 1.83E+06 2.14E+07 1.16E+07 2.86E+06 1.47E+06 1.87E+06 2.16E+06 1.30E+06 1.04E+07 1.89E+06 1.95E+06 3.68E+06 1.96E+06 1.78E+06 1.48E+06 1.67E+06 3.19E+06 1.69E+06 1.70E+06 2.01E+06 2.03E+06 2.24E+06 1.67E+06 2.12E+06 2.35E+06 2.19E+06 2.49E+06 1.76E+06 3.24E+06 4.33E+06 1.43E+06 2.67E+06 2.13E+06 1.31E+06 1.27E+06 1.20E+06 1.35E+06 7.49E+05 9.68E+05 1.64E+06 1.17E+06 1.63E+06 1.21E+06 1.50E+06 5.69E+05 4.72E+05 6.35E+05 5.08E+05 5.46E+05 6.05E+05 7.64E+05 5.66E+05 5.98E+05 5.87E+05 7.48E+05 7.55E+05 6.40E+05 7.52E+05 4.77E+05 4.28E+05 4.70E+05 4.88E+05 6.48E+05 4.42E+05 9.21E+05 1.04E+06 1.07E+06 1.01E+06 8.74E+05 1.84E+06 1.77E+06 1.65E+06 1.72E+06 1.67E+06 5.75E+05 6.61E+05 5.74E+05 6.01E+05 5.62E+05 7.83E+05 5.62E+05 5.55E+05 5.71E+05 6.04E+05 9.43E+05 1.21E+06 7.44E+05 8.49E+05 1.35E+06 2.10E+06 2.25E+06 2.21E+06 1.80E+06 2.31E+06 Alanine 6.07E+03 8.61E+03 6.06E+03 6.53E+03 1.50E+04 4.35E+03 5.65E+03 1.84E+04 1.18E+04 5.32E+03 4.37E+03 4.69E+03 5.14E+03 4.97E+03 6.49E+03 9.58E+03 6.88E+03 6.19E+03 4.47E+03 4.69E+03 6.44E+03 6.95E+03 6.30E+03 6.83E+03 9.60E+03 6.40E+03 6.35E+03 6.31E+03 5.89E+03 6.60E+03 5.20E+03 4.57E+03 4.61E+03 4.42E+03 4.90E+03 5.74E+03 6.43E+03 7.65E+03 4.95E+03 4.58E+03 8.41E+03 7.85E+03 9.17E+03 8.79E+03 7.77E+03 8.18E+03 7.68E+03 7.70E+03 9.50E+03 8.63E+03 Phosphoenolpyruvate 5.44E+04 4.97E+04 5.49E+04 4.52E+04 4.77E+04 4.69E+04 5.02E+04 6.47E+04 5.09E+04 4.82E+04 4.21E+04 4.51E+04 4.69E+04 4.37E+04 4.35E+04 4.90E+04 4.11E+04 4.52E+04 4.57E+04 4.20E+04 2.52E+05 2.12E+05 1.89E+05 2.14E+05 1.73E+05 3.59E+05 3.23E+05 2.60E+05 2.87E+05 2.64E+05 4.76E+04 4.67E+04 4.40E+04 4.39E+04 4.89E+04 4.51E+04 5.27E+04 4.91E+04 4.42E+04 4.36E+04 1.46E+05 1.20E+05 1.05E+05 1.41E+05 1.83E+05 5.54E+05 4.66E+05 4.26E+05 4.32E+05 5.14E+05 Pyruvate 5.35E+05 8.14E+05 2.99E+05 1.88E+05 2.49E+05 2.02E+05 2.61E+05 4.06E+05 2.02E+05 1.47E+05 1.97E+05 2.14E+05 2.22E+05 3.00E+05 3.55E+05 2.29E+06 3.07E+05 1.27E+05 1.00E+05 1.36E+05 2.71E+05 3.19E+05 1.98E+05 4.51E+05 1.54E+06 2.34E+05 3.22E+05 2.67E+05 3.50E+05 3.01E+05 2.58E+05 1.46E+05 1.70E+05 2.45E+05 1.91E+05 1.47E+05 1.25E+06 8.32E+05 2.02E+05 2.06E+05 6.56E+05 5.19E+05 7.77E+05 1.34E+06 1.49E+06 5.42E+05 4.83E+05 5.31E+05 1.42E+06 1.01E+06 Fructose 6-phosphate 9.35E+03 4.08E+03 6.51E+03 4.76E+03 7.76E+03 6.85E+03 1.27E+04 1.07E+04 9.23E+03 6.62E+03 3.37E+03 3.43E+03 2.44E+03 3.21E+03 4.23E+03 2.04E+03 3.94E+03 2.42E+03 2.39E+03 2.71E+03 1.31E+04 2.33E+04 1.13E+04 1.77E+04 1.28E+04 2.67E+05 2.58E+05 2.01E+05 2.08E+05 1.89E+05 2.62E+03 3.55E+03 2.64E+03 3.68E+03 2.54E+03 4.22E+03 2.14E+03 3.68E+03 3.90E+03 3.63E+03 2.82E+04 2.31E+04 1.95E+04 1.51E+04 1.39E+04 2.71E+05 2.98E+05 2.73E+05 2.59E+05 3.07E+05 Ribulose 5-phosphate 1.63E+04 1.67E+04 1.69E+04 1.58E+04 1.33E+04 1.63E+04 1.81E+04 1.80E+04 1.80E+04 1.68E+04 1.48E+04 1.67E+04 1.58E+04 1.49E+04 1.54E+04 1.50E+04 1.30E+04 1.55E+04 1.58E+04 1.41E+04 1.92E+04 1.93E+04 1.51E+04 1.87E+04 1.48E+04 1.47E+05 1.45E+05 1.22E+05 1.24E+05 1.32E+05 8.96E+03 1.16E+04 1.42E+04 1.14E+04 1.20E+04 1.13E+04 1.07E+04 1.20E+04 1.20E+04 1.06E+04 1.68E+04 1.21E+04 8.87E+03 1.10E+04 1.13E+04 1.41E+05 1.21E+05 1.29E+05 1.20E+05 1.50E+05 Ribose 5-phosphate 5.52E+03 7.14E+03 5.65E+03 8.14E+03 5.76E+03 7.62E+03 9.57E+03 7.59E+03 7.80E+03 7.20E+03 6.47E+03 5.62E+03 7.06E+03 6.68E+03 7.21E+03 5.01E+03 3.79E+03 6.76E+03 6.12E+03 6.03E+03 1.32E+04 8.80E+03 8.50E+03 7.15E+03 7.34E+03 7.03E+04 7.09E+04 5.30E+04 5.17E+04 5.04E+04 5.11E+03 6.18E+03 5.15E+03 3.78E+03 4.71E+03 4.74E+03 3.17E+03 4.96E+03 4.99E+03 4.58E+03 1.05E+04 5.78E+03 8.50E+03 5.00E+03 7.41E+03 5.29E+04 4.20E+04 4.94E+04 3.96E+04 5.19E+04 Fructose 1,6-bisphosphate 8.10E+03 1.76E+04 1.13E+04 1.60E+04 6.22E+03 3.10E+04 2.63E+04 3.37E+04 3.54E+04 2.07E+04 9.27E+03 8.76E+03 7.79E+03 5.80E+03 1.28E+04 2.21E+04 9.69E+03 1.49E+04 1.21E+04 1.06E+04 4.14E+04 3.72E+04 2.95E+04 3.80E+04 4.09E+04 2.63E+06 3.04E+06 1.90E+06 2.12E+06 2.33E+06 5.60E+03 1.03E+04 6.15E+03 4.49E+03 5.52E+03 7.63E+03 1.45E+04 1.30E+04 1.56E+04 6.86E+03 4.19E+04 1.62E+04 1.57E+04 2.17E+04 2.42E+04 1.55E+06 1.10E+06 1.09E+06 1.19E+06 1.35E+06 Acetyl-CoA 1.75E+04 1.47E+04 1.48E+04 1.32E+04 1.58E+04 1.90E+04 1.95E+04 2.28E+04 2.20E+04 2.11E+04 8.16E+03 8.63E+03 7.61E+03 7.69E+03 7.50E+03 1.26E+04 1.13E+04 1.15E+04 9.20E+03 1.11E+04 5.60E+04 4.17E+04 5.70E+04 6.28E+04 7.92E+04 1.98E+05 1.65E+05 1.90E+05 1.78E+05 1.78E+05 6.73E+03 1.10E+04 9.08E+03 6.32E+03 6.80E+03 1.31E+04 1.41E+04 1.36E+04 1.33E+04 1.30E+04 1.41E+05 1.13E+05 1.15E+05 1.43E+05 1.48E+05 2.64E+05 2.48E+05 2.54E+05 2.62E+05 3.16E+05 Glyceraldehyde 3-phosphate 4.34E+04 3.00E+04 3.96E+04 3.63E+04 2.52E+04 6.44E+04 8.19E+04 5.99E+04 6.09E+04 5.92E+04 1.43E+04 2.31E+04 1.74E+04 1.88E+04 2.30E+04 2.52E+04 2.16E+04 2.91E+04 2.95E+04 2.83E+04 8.85E+04 9.22E+04 7.29E+04 9.50E+04 8.27E+04 9.37E+05 1.00E+06 1.00E+06 8.13E+05 1.06E+06 1.31E+04 1.66E+04 1.67E+04 1.59E+04 1.38E+04 2.62E+04 2.18E+04 2.43E+04 2.43E+04 2.44E+04 6.95E+04 7.05E+04 3.58E+04 5.47E+04 7.38E+04 6.37E+05 6.62E+05 7.59E+05 5.71E+05 8.18E+05 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name m/z PubChem ID KEGG_ID Glucose 6-phosphate 259 5958 C00668 Fructose 6-phosphate 259 69507 C00085 Fructose 1,6-bisphosphate 339 10267 C05378 Glyceraldehyde 3-phosphate 169 439168 C00118 Phosphoenolpyruvate 167 1005 C00074 Pyruvate 87 107735 C00022 Lactate 89 91435 C00186 Alanine 88 5950 C00041 Acetyl-CoA 808 444493 C00024 Ribulose 5-phosphate 229 439184 C00199 Ribose 5-phosphate 229 439167 C00117 METABOLITES_END #END