{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST002952","ANALYSIS_ID":"AN004848","VERSION":"1","CREATED_ON":"October 31, 2023, 10:31 am"},

"PROJECT":{"PROJECT_TITLE":"Investigate the impact of feeding time on the hexosamine biosynthetic pathway (HBP) in the mouse liver and heart using targeted metabolomics: primary metabolism","PROJECT_SUMMARY":"The overall goal of this project is to advance our understanding of post-translational mechanisms that mediate metabolic regulation of time-of-day-specific protein functions to orchestrate daily rhythms and maintain homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) day-night cycles are key hallmarks of animal health span and are strongly regulated by circadian clocks. Circadian clocks are cell autonomous molecular timers present in the brain and in peripheral organs that enable animals to adapt to predictable daily changes in environment and regulate rhythmic processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, hormonal signaling and neuronal excitability. Besides light, the dominant time cue for the brain clock, metabolic signals from clock-controlled feeding-fasting cycles represent the most potent time cue to entrain and synchronize peripheral clocks in key organs. Much effort has been dedicated to understanding the metabolic regulation of daily biological rhythms, but many important mechanisms are only just emerging. We recently established that metabolic signals from feeding-fasting cycles regulate daily biological rhythms in Drosophila through rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which integrates the metabolites from glucose, amino acid, lipid and nucleotide metabolism. We now propose to investigate whether feeding activity can regulate daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of HBP metabolites in mouse liver and heart are affected by different feeding time within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and heart tissues every 4 hours over a 24-hour period. The liver and heart samples were subjected to targeted metabolomic analysis for HBP metabolites.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Department of Entomology and Nematology","LABORATORY":"Chiu lab","LAST_NAME":"Chiu","FIRST_NAME":"Joanna","ADDRESS":"6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA","EMAIL":"jcchiu@ucdavis.edu","PHONE":"(530) 752-1643"},

"STUDY":{"STUDY_TITLE":"Investigate the impact of feeding time on the hexosamine biosynthetic pathway (HBP) in the mouse liver and heart using targeted metabolomics: primary metabolism","STUDY_SUMMARY":"The overall goal of this project is to advance our understanding of post-translational mechanisms that mediate metabolic regulation of time-of-day-specific protein functions to orchestrate daily rhythms and maintain homeostasis in animals. Robust daily biological rhythms over the 24-hour (h) day-night cycles are key hallmarks of animal health span and are strongly regulated by circadian clocks. Circadian clocks are cell autonomous molecular timers present in the brain and in peripheral organs that enable animals to adapt to predictable daily changes in environment and regulate rhythmic processes such as sleep-wake cycles, feeding-fasting cycles, metabolism, hormonal signaling and neuronal excitability. Besides light, the dominant time cue for the brain clock, metabolic signals from clock-controlled feeding-fasting cycles represent the most potent time cue to entrain and synchronize peripheral clocks in key organs. Much effort has been dedicated to understanding the metabolic regulation of daily biological rhythms, but many important mechanisms are only just emerging. We recently established that metabolic signals from feeding-fasting cycles regulate daily biological rhythms in Drosophila through rhythmic O-linked-N-acetylglucosaminylation (O-GlcNAcylation). Protein O-GlcNAcylation is a nutrient sensitive posttranslational modification (PTM) that is tightly linked to metabolic status, as UDP-GlcNAc, the substrate of O-GlcNAcylation, is produced from hexosamine biosynthetic pathway (HBP), which integrates the metabolites from glucose, amino acid, lipid and nucleotide metabolism. We now propose to investigate whether feeding activity can regulate daily O-GlcNAcylation rhythm in mouse liver and heart and whether the levels of HBP metabolites in mouse liver and heart are affected by different feeding time within a day/night cycle. Here, we restricted the feeding time of C57BL/6 male mice to ZT12-24 (RF12-24. ZT, zeitgeber time; ZT0 indicates light on, while ZT12 indicates light off) v.s. ZT0-12 (RF0-12) for 3 weeks and collected liver and heart tissues every 4 hours over a 24-hour period. The liver and heart samples were subjected to targeted metabolomic analysis for HBP metabolites.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Department of Entomology and Nematology","LABORATORY":"Chiu lab","LAST_NAME":"Chiu","FIRST_NAME":"Joanna","ADDRESS":"6352 Storer Hall, One Shields Avenue, Davis, CA 95616, USA","EMAIL":"jcchiu@ucdavis.edu","PHONE":"(530) 752-1643"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Heart ZT11-rep1_043",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa01_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT11-rep2_044",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa02_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT11-rep3_045",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa03_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT15-rep1_046",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa04_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT15-rep2_047",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa05_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT15-rep3_048",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa06_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT19-rep1_049",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa07_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT19-rep2_050",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa08_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT19-rep3_051",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa09_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT23-rep1_052",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa10_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT23-rep2_053",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa11_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT23-rep3_054",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa12_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT3-rep1_037",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa13_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT3-rep2_038",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa14_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT3-rep3_039",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa15_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT7-rep1_040",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa16_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT7-rep2_041",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa17_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT7-rep3_042",
"Factors":{"organ":"Heart","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa18_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT11-rep1_061",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa19_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT11-rep2_062",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa20_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT11rep3_063",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa21_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT15-rep1_064",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa22_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT15-rep2_065",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa23_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT15-rep3_066",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa24_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT19-rep1_067",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa25_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT19-rep2_068",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa26_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT19-rep3_069",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa27_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT23-rep1_070",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa28_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT23-rep2_071",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa29_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT23-rep3_072",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa30_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT3-rep1_055",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa31_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT3-rep2_056",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa32_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT3-rep3_057",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa33_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT7-rep1_058",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa34_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT7-rep2_059",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa35_1"}
},
{
"Subject ID":"-",
"Sample ID":"Heart ZT7-rep3_060",
"Factors":{"organ":"Heart","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa36_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT11-rep1_007",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa37_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT11-rep2_008",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa38_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT11-rep3_009",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa39_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT15-rep1_010",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa40_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT15-rep2_011",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa41_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT15-rep3_012",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa42_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT19-rep1_013",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa43_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT19-rep2_014",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa44_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT19-rep3_015",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa45_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT23-rep1_016",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa46_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT23-rep2_017",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa47_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT23-rep3_018",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa48_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT3-rep1_001",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa49_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT3-rep2_002",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211227aZTsa50_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT3-rep3_003",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa01_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT7-rep1_004",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa02_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT7-rep2_005",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa03_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT7-rep3_006",
"Factors":{"organ":"Liver","Treatment":"Control"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa04_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT11-rep1_025",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa05_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT11-rep2_026",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa06_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT11-rep3_027",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa07_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT15-rep1_028",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa08_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT15-rep2_029",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa09_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT15-rep3_030",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa10_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT19-rep1_031",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa11_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT19-rep2_032",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa12_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT19-rep3_033",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa13_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT23-rep1_034",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa14_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT23-rep2_035",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa15_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT23-rep3_036",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa16_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT3-rep1_019",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa17_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT3-rep2_020",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa18_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT3-rep3_021",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa19_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT7-rep1_022",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa20_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT7-rep2_023",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa21_1"}
},
{
"Subject ID":"-",
"Sample ID":"Liver ZT7-rep3_024",
"Factors":{"organ":"Liver","Treatment":"Expt"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa22_1"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_001-H",
"Factors":{"organ":"Heart","Treatment":"Heart Pool QC"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa23_1"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_002-H",
"Factors":{"organ":"Heart","Treatment":"Heart Pool QC"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa24_1"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_003-H",
"Factors":{"organ":"Heart","Treatment":"Heart Pool QC"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa25_1"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_001-L",
"Factors":{"organ":"Liver","Treatment":"Liver Pool QC"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa26_1"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_005-L",
"Factors":{"organ":"Liver","Treatment":"Liver Pool QC"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa27_1"}
},
{
"Subject ID":"-",
"Sample ID":"Pool_006-L",
"Factors":{"organ":"Liver","Treatment":"Liver Pool QC"},
"Additional sample data":{"RAW_FILE_NAME":"211228aZTsa28_1"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mice were anesthetized using isoflurane. The liver or heart are dissected out and rinsed with PBS. Then samples were flash frozen in liquid nitrogen and stored at -80C. Samples were weight on dry ice before sending out for metabolomics analysis.","SAMPLE_TYPE":"Heart and Liver"},

"TREATMENT":{"TREATMENT_SUMMARY":"The mice were either fed at natural feeding time (during nighttime, Control group) or at unnatural feeding time (during daytime, Expt group)."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"SOP -GCMS-02252019"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890","COLUMN_NAME":"Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)","SOLVENT_A":"NA","SOLVENT_B":"NA","FLOW_GRADIENT":"NA","FLOW_RATE":"1 mL/min","COLUMN_TEMPERATURE":"NA"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Leco Pegasus IV TOF","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"Mass spectrometer settings: A Leco Pegasus IV time of flight mass spectrometer is controlled by the Leco ChromaTOF software vs. 2.32 (St. Joseph, MI). The transfer line temperature between gas chromatograph and mass spectrometer is set to 280°C. Electron impact ionization at 70V is employed with an ion source temperature of 250°C. Acquisition rate is 17 spectra/second, with a scan mass range of 85-500 Da."},

"MS_METABOLITE_DATA":{
"Units":"ng/mg",

"Data":[{"Metabolite":"fructose-6-phosphate","Heart ZT11-rep1_043":"55.6","Heart ZT11-rep2_044":"72.1","Heart ZT11-rep3_045":"56.5","Heart ZT15-rep1_046":"65.6","Heart ZT15-rep2_047":"82.4","Heart ZT15-rep3_048":"72.7","Heart ZT19-rep1_049":"74.4","Heart ZT19-rep2_050":"84.4","Heart ZT19-rep3_051":"158.0","Heart ZT23-rep1_052":"108.9","Heart ZT23-rep2_053":"67.8","Heart ZT23-rep3_054":"77.8","Heart ZT3-rep1_037":"88.3","Heart ZT3-rep2_038":"67.8","Heart ZT3-rep3_039":"91.4","Heart ZT7-rep1_040":"60.8","Heart ZT7-rep2_041":"69.3","Heart ZT7-rep3_042":"63.3","Heart ZT11-rep1_061":"91.3","Heart ZT11-rep2_062":"66.4","Heart ZT11rep3_063":"85.5","Heart ZT15-rep1_064":"65.9","Heart ZT15-rep2_065":"62.7","Heart ZT15-rep3_066":"95.8","Heart ZT19-rep1_067":"123.2","Heart ZT19-rep2_068":"66.7","Heart ZT19-rep3_069":"65.1","Heart ZT23-rep1_070":"77.9","Heart ZT23-rep2_071":"72.2","Heart ZT23-rep3_072":"77.6","Heart ZT3-rep1_055":"101.0","Heart ZT3-rep2_056":"52.4","Heart ZT3-rep3_057":"90.9","Heart ZT7-rep1_058":"69.6","Heart ZT7-rep2_059":"57.0","Heart ZT7-rep3_060":"65.5","Liver ZT11-rep1_007":"67.7","Liver ZT11-rep2_008":"87.7","Liver ZT11-rep3_009":"90.7","Liver ZT15-rep1_010":"56.5","Liver ZT15-rep2_011":"108.2","Liver ZT15-rep3_012":"73.9","Liver ZT19-rep1_013":"81.7","Liver ZT19-rep2_014":"59.4","Liver ZT19-rep3_015":"79.4","Liver ZT23-rep1_016":"73.3","Liver ZT23-rep2_017":"63.3","Liver ZT23-rep3_018":"58.5","Liver ZT3-rep1_001":"87.6","Liver ZT3-rep2_002":"63.3","Liver ZT3-rep3_003":"88.8","Liver ZT7-rep1_004":"73.0","Liver ZT7-rep2_005":"91.1","Liver ZT7-rep3_006":"63.1","Liver ZT11-rep1_025":"90.1","Liver ZT11-rep2_026":"85.5","Liver ZT11-rep3_027":"91.8","Liver ZT15-rep1_028":"88.4","Liver ZT15-rep2_029":"72.2","Liver ZT15-rep3_030":"74.8","Liver ZT19-rep1_031":"90.9","Liver ZT19-rep2_032":"72.8","Liver ZT19-rep3_033":"83.5","Liver ZT23-rep1_034":"83.8","Liver ZT23-rep2_035":"75.8","Liver ZT23-rep3_036":"91.3","Liver 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