{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST003096","ANALYSIS_ID":"AN005067","VERSION":"1","CREATED_ON":"February 18, 2024, 12:05 am"},

"PROJECT":{"PROJECT_TITLE":"Probiotic intervention of FMT mice","PROJECT_SUMMARY":"L. plantarum intervention of gestational fecal microbiome transplant germ-free mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Bin","FIRST_NAME":"Liu","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"STUDY":{"STUDY_TITLE":"probiotic on gestational health of FMT GF mice","STUDY_SUMMARY":"probiotic L.plantarum intervention gestational on FMT GF mice","INSTITUTE":"Beijing Sanyuan Foods Co. Ltd","LAST_NAME":"Liu","FIRST_NAME":"Bin","ADDRESS":"8th yingchang street, yinghai distric","EMAIL":"liubin@sanyuan.com.cn","PHONE":"01056306594"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENDER":"Female"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"81",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"81.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"107",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"107.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"120",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"120.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"122",
"Factors":{"Sample source":"cecum content","Factor":"control"},
"Additional sample data":{"RAW_FILE_NAME":"122.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"180",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"180.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"185",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"185.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"188",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"188.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"907",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"907.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"908",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"908.mzML"}
},
{
"Subject ID":"-",
"Sample ID":"909",
"Factors":{"Sample source":"cecum content","Factor":"intervention"},
"Additional sample data":{"RAW_FILE_NAME":"909.mzML"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood was collected and centrifuged at 3,000 rpm for 10 min and stored at -80°C environment","SAMPLE_TYPE":"Cecum","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"100 mg of sample was extracted in 50 μL of 15% phosphoric acid with 100 μL of 125 μg/mL 4-methyl valeric acid solution as internal standard and 400 μL ether, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for 1 min and the supernatant was transferred into the vial","PROCESSING_STORAGE_CONDITIONS":"4℃","EXTRACT_STORAGE":"-80℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Thermo Trace 1310","COLUMN_NAME":"Agilent HP-INNOWAX (30 m × 0.25 mm ID × 0.25 μm)","SOLVENT_A":"none","SOLVENT_B":"none","FLOW_GRADIENT":"none","FLOW_RATE":"1 mL/min","COLUMN_TEMPERATURE":"90"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo ISQ LT","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Single ion monitoring (SIM) mode was used with the electron energy of 70 eV.MS Convert software (Proteowizard, v3.0.8789) was applied to convert “.raw” format raw data to “.mzML” format, which would be used for downstream analysis."},

"MS_METABOLITE_DATA":{
"Units":"μg/g",

"Data":[{"Metabolite":"Isobutyric acid","908":"21.1868","188":"25.8283","909":"21.0328","185":"22.4477","180":"20.1322","907":"26.0719","81":"20.7403","107":"15.6469","120":"15.3941","122":"31.5321"},{"Metabolite":"Acetic acid","908":"908.0426","188":"765.4406","909":"776.1381","185":"985.9095","180":"860.3189","907":"927.8182","81":"824.4127","107":"814.7261","120":"1359.5274","122":"1072.7055"},{"Metabolite":"Butyric acid","908":"409.4917","188":"157.2953","909":"166.0075","185":"572.9804","180":"240.3079","907":"223.751","81":"136.8884","107":"140.7733","120":"858.9547","122":"516.5629"},{"Metabolite":"Isovaleric acid","908":"13.9153","188":"18.6662","909":"18.6383","185":"16.795","180":"17.2469","907":"21.9251","81":"16.377","107":"10.2744","120":"9.4717","122":"22.7542"},{"Metabolite":"Valeric acid","908":"58.6347","188":"33.7477","909":"33.4303","185":"62.5157","180":"35.8674","907":"36.7059","81":"25.7836","107":"22.4222","120":"54.6714","122":"65.3222"},{"Metabolite":"Caproic_acid","908":"1.4126","188":"1.3961","909":"1.4772","185":"","180":"1.5641","907":"1.7061","81":"","107":"1.7051","120":"1.8658","122":"1.7499"},{"Metabolite":"Propionic acid","908":"385.3268","188":"236.7324","909":"315.5355","185":"458.8674","180":"271.14","907":"335.4233","81":"241.0505","107":"182.3167","120":"447.7459","122":"420.8304"}],

"Metabolites":[{"Metabolite":"Isobutyric acid","retention index":"6.1","quantified m/z":"0","PubChem ID":"6590","KEGG ID":"C02632"},{"Metabolite":"Acetic acid","retention index":"4.64","quantified m/z":"0","PubChem ID":"176","KEGG ID":"C00033"},{"Metabolite":"Butyric acid","retention index":"6.98","quantified m/z":"0","PubChem ID":"264","KEGG ID":"C00246"},{"Metabolite":"Isovaleric acid","retention index":"7.64","quantified m/z":"0","PubChem ID":"10430","KEGG ID":"C08262"},{"Metabolite":"Valeric acid","retention index":"8.77","quantified m/z":"0","PubChem ID":"7991","KEGG ID":"C00803"},{"Metabolite":"Caproic_acid","retention index":"10.23","quantified m/z":"0","PubChem ID":"8892","KEGG ID":"C01585"},{"Metabolite":"Propionic acid","retention index":"5.7","quantified m/z":"0","PubChem ID":"1032","KEGG ID":"D02310"}]
}

}