#METABOLOMICS WORKBENCH stephensondj_20240227_094013 DATATRACK_ID:4676 STUDY_ID:ST003108 ANALYSIS_ID:AN005090 PROJECT_ID:PR001931
VERSION             	1
CREATED_ON             	February 27, 2024, 10:57 am
#PROJECT
PR:PROJECT_TITLE                 	Complete absence of GLUT1 does not impair human terminal erythroid
PR:PROJECT_TITLE                 	differentiation
PR:PROJECT_SUMMARY               	The Glucose transporter 1 (GLUT1) is one of the most abundant proteins within
PR:PROJECT_SUMMARY               	the erythrocyte membrane and is required for glucose and dehydroascorbic acid
PR:PROJECT_SUMMARY               	(Vitamin C precursor) transport. It is widely recognized as a key protein for
PR:PROJECT_SUMMARY               	red cell structure, function, and metabolism. Previous reports highlighted the
PR:PROJECT_SUMMARY               	importance of GLUT1 activity within these uniquely glycolysis-dependent cells,
PR:PROJECT_SUMMARY               	in particular for increasing antioxidant capacity needed to avoid irreversible
PR:PROJECT_SUMMARY               	damage from oxidative stress in humans. However, studies of glucose transporter
PR:PROJECT_SUMMARY               	roles in erythroid cells are complicated by species-specific differences between
PR:PROJECT_SUMMARY               	humans and mice. Here, using CRISPRmediated gene editing of immortalized
PR:PROJECT_SUMMARY               	erythroblasts and adult CD34+ hematopoietic progenitor cells, we generate
PR:PROJECT_SUMMARY               	committed human erythroid cells completely deficient in expression of GLUT1. We
PR:PROJECT_SUMMARY               	show that absence of GLUT1 does not impede human erythroblast proliferation,
PR:PROJECT_SUMMARY               	differentiation, or enucleation. This work demonstrates for the first-time
PR:PROJECT_SUMMARY               	generation of enucleated human reticulocytes lacking GLUT1. The GLUT1-deficient
PR:PROJECT_SUMMARY               	reticulocytes possess no tangible alterations to membrane composition or
PR:PROJECT_SUMMARY               	deformability in reticulocytes. Metabolomic analyses of GLUT1-deficient
PR:PROJECT_SUMMARY               	reticulocytes reveal hallmarks of reduced glucose import, downregulated
PR:PROJECT_SUMMARY               	metabolic processes and upregulated AMPK-signalling, alongside alterations in
PR:PROJECT_SUMMARY               	antioxidant metabolism, resulting in increased osmotic fragility and metabolic
PR:PROJECT_SUMMARY               	shifts indicative of higher oxidant stress. Despite detectable metabolic changes
PR:PROJECT_SUMMARY               	in GLUT1 deficient reticulocytes, the absence of developmental phenotype,
PR:PROJECT_SUMMARY               	detectable proteomic compensation or impaired deformability comprehensively
PR:PROJECT_SUMMARY               	alters our understanding of the role of GLUT1 in red blood cell structure,
PR:PROJECT_SUMMARY               	function and metabolism. It also provides cell biological evidence supporting
PR:PROJECT_SUMMARY               	clinical consensus that reduced GLUT1 expression does not cause anaemia in GLUT1
PR:PROJECT_SUMMARY               	deficiency syndrome.
PR:INSTITUTE                     	University of Colorado
PR:LAST_NAME                     	Stephenson
PR:FIRST_NAME                    	Daniel
PR:ADDRESS                       	Research 1 South L18-1303 12801 E. 17th Ave., Aurora, Colorado, 80045, USA
PR:EMAIL                         	daniel.stephenson@cuanschutz.edu
PR:PHONE                         	303-724-3339
#STUDY
ST:STUDY_TITLE                   	Complete absence of GLUT1 does not impair human terminal erythroid
ST:STUDY_TITLE                   	differentiation
ST:STUDY_SUMMARY                 	The Glucose transporter 1 (GLUT1) is one of the most abundant proteins within
ST:STUDY_SUMMARY                 	the erythrocyte membrane and is required for glucose and dehydroascorbic acid
ST:STUDY_SUMMARY                 	(Vitamin C precursor) transport. It is widely recognized as a key protein for
ST:STUDY_SUMMARY                 	red cell structure, function, and metabolism. Previous reports highlighted the
ST:STUDY_SUMMARY                 	importance of GLUT1 activity within these uniquely glycolysis-dependent cells,
ST:STUDY_SUMMARY                 	in particular for increasing antioxidant capacity needed to avoid irreversible
ST:STUDY_SUMMARY                 	damage from oxidative stress in humans. However, studies of glucose transporter
ST:STUDY_SUMMARY                 	roles in erythroid cells are complicated by species-specific differences between
ST:STUDY_SUMMARY                 	humans and mice. Here, using CRISPRmediated gene editing of immortalized
ST:STUDY_SUMMARY                 	erythroblasts and adult CD34+ hematopoietic progenitor cells, we generate
ST:STUDY_SUMMARY                 	committed human erythroid cells completely deficient in expression of GLUT1. We
ST:STUDY_SUMMARY                 	show that absence of GLUT1 does not impede human erythroblast proliferation,
ST:STUDY_SUMMARY                 	differentiation, or enucleation. This work demonstrates for the first-time
ST:STUDY_SUMMARY                 	generation of enucleated human reticulocytes lacking GLUT1. The GLUT1-deficient
ST:STUDY_SUMMARY                 	reticulocytes possess no tangible alterations to membrane composition or
ST:STUDY_SUMMARY                 	deformability in reticulocytes. Metabolomic analyses of GLUT1-deficient
ST:STUDY_SUMMARY                 	reticulocytes reveal hallmarks of reduced glucose import, downregulated
ST:STUDY_SUMMARY                 	metabolic processes and upregulated AMPK-signalling, alongside alterations in
ST:STUDY_SUMMARY                 	antioxidant metabolism, resulting in increased osmotic fragility and metabolic
ST:STUDY_SUMMARY                 	shifts indicative of higher oxidant stress. Despite detectable metabolic changes
ST:STUDY_SUMMARY                 	in GLUT1 deficient reticulocytes, the absence of developmental phenotype,
ST:STUDY_SUMMARY                 	detectable proteomic compensation or impaired deformability comprehensively
ST:STUDY_SUMMARY                 	alters our understanding of the role of GLUT1 in red blood cell structure,
ST:STUDY_SUMMARY                 	function and metabolism. It also provides cell biological evidence supporting
ST:STUDY_SUMMARY                 	clinical consensus that reduced GLUT1 expression does not cause anaemia in GLUT1
ST:STUDY_SUMMARY                 	deficiency syndrome.
ST:INSTITUTE                     	University of Colorado
ST:LAST_NAME                     	Stephenson
ST:FIRST_NAME                    	Daniel
ST:ADDRESS                       	Research 1 South L18-1303 12801 E. 17th Ave., Aurora, Colorado, 80045, USA
ST:EMAIL                         	daniel.stephenson@cuanschutz.edu
ST:PHONE                         	303-724-3339
#SUBJECT
SU:SUBJECT_TYPE                  	Cultured cells
SU:SUBJECT_SPECIES               	Homo sapiens
SU:TAXONOMY_ID                   	9606
#SUBJECT_SAMPLE_FACTORS:         	SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Raw file names and additional sample data
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-001+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-001+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-002+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-002+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-003+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-003+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-004+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-004+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-005+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-005+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-006+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-006+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-007+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-007+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-008+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-008+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-009+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-009+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-010+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-010+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-011+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-011+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-012+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-012+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-013+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-013+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-014+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-014+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-015+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-015+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-016+	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-016+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-017+	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-017+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-018+	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-018+.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-001-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-001-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-002-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-002-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-003-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-003-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-004-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-004-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-005-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-005-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-006-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-006-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-007-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-007-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-008-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-008-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-009-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-009-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-010-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-010-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-011-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-011-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-012-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-012-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-013-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-013-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-014-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-014-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-015-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-015-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-016-	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-016-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-017-	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-017-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-018-	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-018-.mzXML; Pellet size=10 Million; Analysis=Metabolomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-001L+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-001L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-002L+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-002L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-003L+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-003L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-004L+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-004L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-005L+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-005L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-006L+	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-006L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-007L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-007L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-008L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-008L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-009L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-009L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-010L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-010L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-011L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-011L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-012L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-012L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-013L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-013L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-014L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-014L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-015L+	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-015L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-016L+	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-016L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-017L+	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-017L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-018L+	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-018L+.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Positive
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-001L-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-001L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 1	DS1-095-002L-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-002L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-003L-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-003L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 4	DS1-095-004L-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-004L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-005L-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-005L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor 5	DS1-095-006L-	Sample source:cultured reticulocytes | Assay:04CF29	RAW_FILE_NAME(Raw File Name)=DS1-095-006L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-007L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-007L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-008L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-008L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-009L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-009L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-010L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-010L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-011L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-011L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-012L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-012L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-013L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-013L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-014L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-014L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-015L-	Sample source:cultured reticulocytes | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-015L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor A	DS1-095-016L-	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-016L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor C	DS1-095-017L-	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-017L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
SUBJECT_SAMPLE_FACTORS           	Donor D	DS1-095-018L-	Sample source:Red blood cells | Assay:01CF19	RAW_FILE_NAME(Raw File Name)=DS1-095-018L-.RAW; Pellet size=10 Million; Analysis=Lipidomics; Polarity=Negative
#COLLECTION
CO:COLLECTION_SUMMARY            	For flow cytometry, cells were fixed (1% paraformaldehyde, 0.0075%
CO:COLLECTION_SUMMARY            	glutaraldehyde) to prevent antibody agglutination, except for assays using
CO:COLLECTION_SUMMARY            	GLUT1.RBD. Samples were labelled with primary antibodies in PBSAG (PBS with 1%
CO:COLLECTION_SUMMARY            	(w/v) Glucose and 0.5%(w/v) |Bovine Serum Albumin (BSA)) supplemented with extra
CO:COLLECTION_SUMMARY            	1%(w/v) BSA for 30min, in the dark. METAFORA’s GLUT1.RBD was incubated at
CO:COLLECTION_SUMMARY            	37°C and all remaining antibodies incubated at 4°C. Cells were washed 2x PBSAG
CO:COLLECTION_SUMMARY            	and, if required, incubated with appropriate secondary antibody under the same
CO:COLLECTION_SUMMARY            	conditions as described for primary. Cells were washed 2x with PBSAG and
CO:COLLECTION_SUMMARY            	analysed on a Miltenyi MACSQuant 10 flow cytometer. Data was analysed using
CO:COLLECTION_SUMMARY            	FlowJo v10.7 (FlowJo). Reticulocytes were identified by gating on the
CO:COLLECTION_SUMMARY            	Hoechst-negative population. 4 Cells were sorted using a BD Influx Cell Sorter
CO:COLLECTION_SUMMARY            	(BD Biosciences). BEL-A CRISPR edited populations were single cell sorted based
CO:COLLECTION_SUMMARY            	on viability (DRAQ7 negativity). Primary GLUT1 KO cultures were sorted on days 6
CO:COLLECTION_SUMMARY            	or 7 of differentiation using DRAQ7 and the eGFP-fused GLUT1.RBD to purify the
CO:COLLECTION_SUMMARY            	negative population with a gate based on non-targeting guide control cells.
CO:SAMPLE_TYPE                   	Cultured cells
#TREATMENT
TR:TREATMENT_SUMMARY             	Filtered reticulocytes (1-2 x105/well) were incubated in decreasing NaCl
TR:TREATMENT_SUMMARY             	concentrations (0.9–0%) for 10min at 37°C. Lysis was stopped by adding 4x
TR:TREATMENT_SUMMARY             	volume of PBSAG. Live cells, considered as having a normal FSC/SSC profile as
TR:TREATMENT_SUMMARY             	defined by the 0.9% NaCl control, were counted by flow cytometry using the
TR:TREATMENT_SUMMARY             	MACSQuant10
#SAMPLEPREP
SP:SAMPLEPREP_SUMMARY            	Extraction of metabolites and lipids from red cell pellets was as follows: 36
SP:SAMPLEPREP_SUMMARY            	µL of cold MeOH:MeCN:H2O (5:3:2, v:v:v) was added to all pellets (pellet size
SP:SAMPLEPREP_SUMMARY            	pre-determined to be 10 million prior to extraction). Samples were vortexed at
SP:SAMPLEPREP_SUMMARY            	4°C for 30 minutes. Insoluble material was pelleted by centrifugation (18000g,
SP:SAMPLEPREP_SUMMARY            	10 min) and supernatants were isolated for analysis by UHPLC-MS for metabolomics
SP:SAMPLEPREP_SUMMARY            	and lipidomics.
#CHROMATOGRAPHY
CH:CHROMATOGRAPHY_SUMMARY        	Lipidomics Negative
CH:CHROMATOGRAPHY_TYPE           	Reversed phase
CH:INSTRUMENT_NAME               	Thermo Vanquish
CH:COLUMN_NAME                   	Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um)
CH:SOLVENT_A                     	75:25 H2O:ACN 5mM NH4OAc
CH:SOLVENT_B                     	90:10 iPrOH:ACN 5mM NH4OAc
CH:FLOW_GRADIENT                 	0 min - 0.3ml/min - 10%B, 3 min - 0.3ml/min - 95%B, 4.2min - 0.3ml/min - 95%B,
CH:FLOW_GRADIENT                 	4.3min - 0.45ml/min - 10%B, 4.9min - 0.4ml/min - 10%B, 5 min - 0.3ml/min 10%B
CH:FLOW_RATE                     	0.3-0.45ml/min
CH:COLUMN_TEMPERATURE            	50
#ANALYSIS
AN:ANALYSIS_TYPE                 	MS
#MS
MS:INSTRUMENT_NAME               	Thermo Q Exactive Orbitrap
MS:INSTRUMENT_TYPE               	Orbitrap
MS:MS_TYPE                       	ESI
MS:ION_MODE                      	NEGATIVE
MS:MS_COMMENTS                   	Lipidsearch used for data analysis
#MS_METABOLITE_DATA
MS_METABOLITE_DATA:UNITS	area
MS_METABOLITE_DATA_START
Samples	DS1-095-001L-	DS1-095-002L-	DS1-095-003L-	DS1-095-004L-	DS1-095-005L-	DS1-095-006L-	DS1-095-007L-	DS1-095-008L-	DS1-095-009L-	DS1-095-010L-	DS1-095-011L-	DS1-095-012L-	DS1-095-013L-	DS1-095-014L-	DS1-095-015L-	DS1-095-016L-	DS1-095-017L-	DS1-095-018L-
Factors	Sample source:cultured reticulocytes | Assay:04CF29	Sample source:cultured reticulocytes | Assay:04CF29	Sample source:cultured reticulocytes | Assay:04CF29	Sample source:cultured reticulocytes | Assay:04CF29	Sample source:cultured reticulocytes | Assay:04CF29	Sample source:cultured reticulocytes | Assay:04CF29	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:cultured reticulocytes | Assay:01CF19	Sample source:Red blood cells | Assay:01CF19	Sample source:Red blood cells | Assay:01CF19	Sample source:Red blood cells | Assay:01CF19
Cer(d18:2_17:0)	56922	36784	47592	27847	80311	25575	61137	66348	55706	132845	119265	29783	83597	43959	104051	7759	7003	11319
FA(27:0)	7200	7973	12422	10207	8560	11204	9715	8711	10456	10156	12127	9153	8929	9646	5932	10991	10423	6937
FA(15:0)	313685	257106	312069	289128	326089	292222	525857	546080	541150	528935	558797	522721	515482	522088	525392	555398	539313	544269
FA(26:0)	30268	28596	32596	27982	35934	27800	30899	40526	33699	31358	36277	35360	33180	32645	34435	27286	30390	28917
FA(29:0)	8609	11145	10134	8971	11169	8839	9115	10627	10079	9520	8273	9555	9365	10609	9764	8979	8833	8110
FA(30:0)	14750	18170	15543	16647	17542	15389	18365	18753	16618	19519	17652	15185	16692	16343	19821	16189	13193	19335
FA(28:0)	13613	14351	15533	15917	13954	13278	10957	11169	13809	15816	12600	15302	15436	13314	15578	14071	10790	11819
FA(16:1)	56488	65596	161129	57543	60113	44274	131158	131721	142791	64255	126377	150509	87567	128198	125356	111478	126957	64630
FA(19:0)	38870	30654	36471	27679	37170	10413	37003	31511	35130	26958	12851	36687	32908	38240	12266	25546	24849	30906
FA(18:3)	12620	12000	11717	11716	12296	13361	10992	12157	14722	8962	9961	16075	11471	16505	11482	8579	12091	7196
FA(18:2)	246335	238264	278096	269205	252029	260516	396641	361441	459395	293293	313148	592231	434397	538184	396962	361472	374932	287836
FA(20:5)	5414	8344	15989	7346	9580	5992	11706	10746	8416	12134	24096	11163	8033	8333	6960	3841	3337	3015
FA(20:4)	121273	123400	92522	129867	110101	117164	139222	137662	114792	154559	164036	210373	140914	154478	139112	60844	54785	55882
FA(20:3)	48385	43541	43555	57833	59686	36143	39626	46975	37900	28512	36068	74920	46994	103150	39595	20459	24698	22326
FA(22:6)	15613	15984	9070	15555	10256	13355	9858	9285	9389	15220	15176	22727	8711	9918	11929	2945	2189	3976
FA(22:5)	59519	55011	27444	55049	36203	43831	27403	25873	25415	34376	35459	48932	12618	13499	16476	4661	5773	6283
FA(22:4)	83408	80473	46352	83108	56003	60033	47427	44740	40905	45889	37406	71142	23162	21955	32555	7514	7993	10379
FA(24:0)	76474	77458	86397	79921	90428	75309	87735	92711	73517	121159	118340	85902	115043	101139	113416	63286	65785	63350
FA(28:7)	330950	523265	448528	153774	253500	326756	806430	1496594	578479	453260	1354628	554519	533982	555792	989004	218751	97384	206803
FA(20:2)	16548	15648	17935	17793	19822	16865	14779	14740	12244	12800	17063	15305	19144	20450	17210	5092	4707	6371
FA(22:0)	84545	88392	89842	75222	90962	81739	86737	87540	78895	115990	114805	77125	115129	105917	102458	62789	65006	67856
FA(20:1)	19575	17217	22148	16114	20543	17025	17834	16785	16264	17606	22551	21900	24755	23601	21257	9150	9979	12044
FA(15:1)	15385	23134	31739	19940	31424	24129	12285	12763	10717	11979	11997	9605	11506	9961	12345	13165	11926	12699
FA(17:1)	57813	54766	64019	59853	52986	55480	59966	57258	66838	52156	57413	64238	60857	67990	55258	47112	50588	47746
FA(22:3)	15507	14437	12632	11997	13549	11673	8141	10712	7595	26473	41237	37020	28818	25122	32403	550	751	1346
FA(24:1)	83342	82012	56220	60598	88679	56608	41677	50035	28957	102429	108596	61049	55287	48811	58370	10857	10616	13899
FA(22:1)	8641	8833	7253	7916	10782	8854	8348	8824	7190	10102	15928	10338	10257	9953	11352	6055	5410	7027
FA(24:2)	8677	7654	5363	7184	7973	7399	4762	5781	3479	8615	10487	8718	3374	3559	4557	2125	1727	2114
FA(16:3)	3279	5324	2740	2984	6691	4240	12377	10755	10752	13488	7185	4020	4987	3539	7367	20943	16597	20356
FA(18:0)	7658838	7668562	8477297	7053849	9478755	8672844	4639085	4466408	4383089	4007083	7147329	3530875	3571918	4395028	4157706	4508699	4221815	4836417
FA(17:0)	251142	239632	295689	236964	281142	253121	234975	219816	66315	198932	225418	62758	227586	238597	212342	189112	209630	195407
FA(20:0)	106693	90824	104626	94523	132741	107296	72317	72520	72721	66972	111100	59348	76451	79641	79992	65699	65245	73590
FA(23:0)	24562	28003	24136	26137	28068	25530	26501	28132	22703	25592	26921	11970	37489	28798	29790	25797	24358	23108
FA(18:1)	870468	672394	1218576	704462	775295	743960	735265	672880	738218	579228	734361	842026	698823	915691	641323	548599	593189	568969
FA(14:0)	726533	877266	1004729	729377	1009918	778769	239459	259357	246049	284919	10437	6639	3068	206124	223230	386562	306709	314123
FA(12:0)	7852	199801	215399	6205	1490486	1238	238189	218892	1473439	253633	244981	137190	3234	141759	167523	510958	301286	327908
FA(21:0)	29934	30469	26227	27546	32078	27951	28531	31935	27694	28475	29136	23099	28158	24745	27924	26136	24950	27669
FA(25:0)	18202	19421	16485	20018	22794	19771	16572	21731	22763	14839	17137	17293	18631	19438	13516	21217	19419	16495
LPC(O-19:1)	19867	18616	22565	21103	26787	19370	19602	20950	15642	13686	16095	13482	16769	18028	18760	3391	4669	5703
LPC(O-19:0)	16222	13236	17721	15702	18959	16583	20781	26053	19663	17334	19202	17975	49323	49129	45431	11212	13955	14662
LPC(19:2)	28822	31671	40575	43096	39773	35918	60869	58370	59144	46342	44116	50879	56763	48631	48519	52632	61774	63913
LPC(19:1)	116752	127691	134676	140668	151084	144189	172131	141502	167011	123130	123948	121027	145831	151478	145009	130762	146318	157827
LPE(16:0)	70665	82233	53202	69215	77479	85636	98280	89280	52637	99176	102186	62256	41354	35908	46767	24418	19584	29118
LPE(18:0)	72972	91408	89098	80599	106664	85561	109674	89388	74069	120678	120723	66525	84233	66791	88893	20840	19401	24920
LPE(18:1)	27871	26065	17838	18914	50028	23619	21014	25189	25754	19811	16730	14410	33575	20310	28070	15997	12672	24115
LPE(20:3)	6711	6922	10578	7548	11324	8430	9745	16781	6043	14512	20361	12612	29778	36879	30531	14428	14700	5076
LPE(20:4)	91170	136774	69392	80307	103970	76821	97710	101349	91193	142033	76821	54167	107301	147420	78343	45505	46096	61712
LPE(22:3)	21576	20577	15627	10098	24330	17144	18512	33604	11718	37460	28044	13134	48698	49360	41832	15638	10308	8644
LPE(22:4)	80488	90756	45805	42966	98255	46418	45368	48159	67819	36822	51979	22976	52696	32265	23533	12768	10535	27737
LPE(22:5)	32908	21335	11608	37473	44825	36378	23163	34182	21778	18265	25514	13475	20490	16241	8905	19932	14772	22592
LPE(P-18:1)	27256	35657	16360	32155	31805	30146	23338	22200	14990	39207	50313	49623	27014	22740	29050	2049	1592	3794
LPE(P-18:0)	73503	66503	46611	64012	75093	55946	58290	64208	39531	128002	198778	175574	176808	174657	240275	5547	4841	8249
LPG(20:3)	10486	8940	9093	12180	4231	9141	7108	11897	6656	9790	11309	8492	15633	36894	18907	1	1	74
LPG(22:4)	24986	28495	15983	22728	9895	14051	14264	12909	19022	32497	47171	60084	11880	10440	19662	2	2	2
LPG(22:5)	9690	28492	9027	24770	9571	12092	8045	9934	11500	18803	21119	29911	3968	3302	8481	1	1	1
LPG(18:1)	15958	16679	13487	16321	19796	16116	15830	16105	15758	19410	13881	15322	19800	14985	42639	340	400	528
LPG(20:4)	13023	16298	5994	11396	7343	9046	7797	8869	6615	19404	25627	15741	24248	21768	29945	72	97	87
LPI(18:0)	129039	98045	144960	110621	250776	135632	119780	152583	113510	154921	242140	202662	10048	295216	351370	25633	27380	31935
LPI(20:3)	2389	1314	1478	2839	5571	3860	2909	3989	2598	8532	13084	13201	19478	19553	18476	501	507	564
LPS(18:0)	60869	67670	44180	41997	86441	58592	75159	73857	49160	79469	112805	69440	68169	60655	72321	20847	15932	22304
PA(16:0_18:2)	8898	4006	9167	4329	9264	3541	6790	7218	5366	10079	7447	2941	11245	5342	12353	17676	13612	18269
PA(16:0_20:4)	9852	10240	11813	6738	10826	5158	12441	10232	8250	21695	17429	5762	16588	11562	15632	9626	9006	17641
PC(O-14:1_22:4)	1237856	610056	1299951	375911	1134673	235821	711223	1066281	566862	823156	1093246	131561	751648	362567	1552546	505055	395497	1822756
PC(O-19:2_20:4)	127334	128185	133564	156949	180748	119012	135323	163492	103810	82413	120538	92917	93122	85780	113695	29225	19727	34389
PC(16:0_18:2)	226142	157563	301490	95896	268137	74996	159441	198449	183405	172151	172562	56918	170566	102382	337771	402074	422455	627202
PC(16:0_17:0)	384556	203210	306451	166153	378999	111190	332661	372504	175570	466922	301486	52874	125126	47743	158230	104570	71865	244849
PC(17:0_18:2)	2580667	2070774	2595599	1726982	2917114	1694530	2344528	2513880	2329662	1605168	1482480	841899	1993520	1512888	2265258	3837008	4113940	3586662
PC(O-16:1_22:4)	540547	277592	478727	116137	397049	39616	128096	285688	131727	336065	357145	34996	125650	44108	268557	79790	71031	284526
PC(O-19:1_20:4)	623136	597321	427590	433745	877034	530395	452893	643487	442683	349869	510663	313193	586403	364502	728973	59754	64681	87617
PC(19:0_22:6)	61671	65853	49427	63788	61103	50659	81162	78712	75376	81897	75385	48694	55617	57391	63137	61280	47377	115638
PC(16:0_20:4)	109768	86158	124425	83333	150351	107824	124592	131501	78745	124233	154594	112560	139495	67138	156264	26076	20251	86329
PC(O-19:0_18:1)	20950	7380	20668	4335	21985	2514	8847	14427	7158	15548	23787	1713	31710	15722	71763	4094	2793	19787
PC(O-14:1_18:2)	22344	8441	22358	6963	20759	5499	10993	17193	12335	27798	31086	8493	24682	17636	36904	22929	13424	69472
PC(O-14:1_18:1)	67929	27984	74646	12608	52484	11600	28182	51591	24961	59811	68944	7982	27429	15551	62206	29711	25676	141325
PC(O-16:1_18:1)	21208	6508	15756	2011	10369	1435	4433	7638	3294	10861	33974	1719	6439	4060	31219	5715	4075	50074
PC(16:0_18:1)	196437	149626	161049	106777	157131	105514	167570	177511	136200	147315	142635	68251	165561	105848	200213	124478	101329	246745
PC(O-17:0_18:1)	394834	226042	317263	142815	433314	145946	256225	352096	217045	248892	276798	70703	308688	189837	391460	50914	47558	112599
PC(17:0_18:1)	4321375	2828929	3219618	1996306	3456340	1842417	2741126	3217860	2609784	3080307	2890890	1016624	2241628	1478041	2951611	2024004	1872892	2824332
PC(18:0_18:2)	62304	43653	48220	36898	71374	24534	59106	82635	65674	41054	48291	17584	106345	84435	93940	110525	117399	169375
PC(9:0_18:0)	43416	34314	29044	34140	46967	33954	57244	68479	56292	47319	46405	36764	60966	57377	64203	9474	9510	10625
PC(O-14:1_20:4)	504169	354300	614382	238448	672767	169208	421928	581024	430020	366606	355833	111237	277648	145072	476032	386550	253784	1330981
PC(17:0_16:1)	332042	238690	252773	188387	347831	167916	249656	253604	289635	173718	213291	120533	201750	188806	241238	129219	119537	178296
PC(18:2_19:2)	2845882	2417690	1974087	2315896	68071	2220539	98664	2976982	2093212	3393089	3337753	2197542	3114353	1850586	3161247	1081847	540439	1865184
PC(O-19:2_18:1)	99365	76471	90059	57493	120115	56455	61806	94054	62644	60066	101774	41322	189128	196021	230503	29225	35326	49179
PC(17:0_20:3)	882444	700318	927944	688821	1046288	634514	872395	1056697	781809	909767	1109610	475560	1141442	1052311	1046177	1023801	1232375	1353618
PC(18:0_20:4)	69579	63182	46842	40961	59822	51313	64747	65616	74948	77800	105149	36683	147069	91605	141083	18441	14765	48280
PC(19:2_20:4)	368384	322515	281095	247763	309510	96702	104949	426823	267220	110679	337681	214188	367627	287231	359332	328216	234672	514755
PC(19:0_22:4)	74821	45970	53622	32689	45740	17220	49554	60606	61103	92447	66635	15694	44582	23394	66939	12522	9673	34643
PC(O-16:1_22:3)	86521	34112	75941	8452	60333	6243	21861	33233	11356	104463	77694	4500	55545	28557	68007	11657	7035	28431
PC(18:0_18:1)	91376	46592	68762	34381	69507	25600	68015	82353	54613	76933	65927	16747	108599	55610	138466	52770	36131	116740
PC(17:0_20:5)	895391	439022	732393	261488	643657	194075	475044	601146	367134	509493	469346	177452	286221	200823	374857	212252	141200	860692
PC(19:0_18:1)	269118	139442	263834	77064	236865	50734	181297	219917	141405	337663	268902	25402	295230	128191	466229	308347	264156	897416
PC(21:4_20:4)	85685	60634	47442	35437	68172	29051	68412	66085	38065	199858	149961	50086	82954	44935	66757	20547	19911	48310
PC(16:0_20:3)	17223	13586	20664	12020	16273	11753	11805	18777	12615	11031	16147	3471	23386	19792	31341	11890	550	24322
PC(18:1_18:2)	118024	45646	165889	27262	58387	10898	26590	40886	37249	192871	195708	21595	94843	57229	223187	17762	25848	23776
PC(O-17:0_20:4)	188000	164582	228646	200136	318863	184625	263562	302232	202979	162468	1	161693	320566	228778	355424	95877	62027	206860
PC(O-19:1_18:1)	229932	100276	187158	76172	242640	544	136970	192049	127594	97929	130628	36041	183149	132022	174599	17844	24008	39769
PC(17:0_22:4)	987265	849319	692080	638191	899948	566188	824489	833975	757569	1356157	1071301	478938	1114892	783860	1304840	435637	346588	935489
PC(O-17:0_18:2)	80566	71344	83655	43270	107579	59759	69508	90570	67778	53547	70993	35694	105580	81052	130812	70310	56812	126561
PC(O-19:0_20:4)	420619	253779	455046	181381	439282	125313	305904	399958	254417	327419	443493	111219	676477	411863	787444	40494	38686	107191
PC(O-17:1_18:2)	19158	18538	17193	19572	27467	18909	22487	30282	22253	17854	20283	11096	34084	28817	41177	52423	60861	87285
PC(O-16:2_22:6)	168101	109978	110340	83965	152872	71749	78155	142101	83498	85989	87027	20469	30977	20515	43451	112697	78430	197976
PC(O-17:1_20:4)	99055	110221	76005	72459	112417	71253	85531	107633	86790	65909	73262	41376	78635	61326	84828	67125	48350	120046
PC(18:1_19:1)	576037	415300	557424	320907	659526	277326	529806	602891	492785	494983	437288	146016	629331	437259	762003	1361993	1404180	1835164
PC(19:1_20:4)	437563	423755	346727	418239	454678	353160	505409	518530	435462	552325	481891	326254	490953	391732	475628	261176	187981	450631
PC(6:0_16:1)	20437	16888	11436	11792	16708	13109	28402	23853	25677	16429	17287	16772	23635	21284	24097	1452	1346	1443
PC(O-16:2_22:4)	637432	322226	530857	189072	385714	102962	225792	339420	214416	369160	354212	37542	91036	36297	220860	153334	155870	470533
PC(17:1_18:2)	178974	134201	185594	118639	209917	124099	165560	176313	83398	143875	144554	81356	166501	154659	196065	145933	167374	181074
PC(13:1_18:1)	35687	5863	44213	4369	41466	709	16896	22115	13261	14590	13284	1782	13942	4494	24319	495	848	25495
PE(16:0_18:1)	261667	138075	272065	72878	254835	60032	147949	197985	133886	308913	288736	48131	80396	40002	179404	277009	198564	683286
PE(16:0_18:2)	199497	100712	222733	83135	217410	64277	132567	155775	133195	217022	164605	41363	145590	101499	236855	295362	275686	326107
PE(16:0_20:4)	2921299	2000686	2232456	1326714	2200080	1152677	2077832	2247461	1577310	2974798	2408743	684652	1141936	517148	1658859	849391	771214	1608079
PE(18:0_18:1)	119901	76331	111540	47611	97746	47349	79191	91782	67641	105358	110365	26896	61788	39486	93550	68797	59315	97901
PE(18:0_20:4)	1989369	1093975	1839386	685679	1747220	510941	1392325	1436353	1049528	2244433	1580355	297462	1156210	412336	1588166	483661	396509	1123769
PE(18:1_18:1)	140427	58959	186003	38943	145302	74234	70536	101922	80055	123916	118294	23815	105796	48588	226772	271206	307833	546682
PE(18:1_20:4)	1520957	888432	1239907	786903	1644496	643025	1560581	1525483	1088019	1177280	1048242	358657	926599	423470	1328836	841195	911916	1609662
PE(O-16:1_18:1)	67112	25547	82302	11656	51746	10371	29645	51602	24017	60649	68647	7957	25284	15608	64377	31034	24175	127219
PE(O-16:1_18:2)	22232	8449	22377	6964	20765	5500	10987	17201	12341	29869	31086	8499	24663	18935	36928	22925	13438	74333
PE(O-16:1_20:3)	55719	29745	75186	23831	99717	13478	24099	43058	22882	60174	71551	15185	102815	61920	129804	80676	66263	175246
PE(O-16:1_20:4)	560220	373445	616317	261825	672472	187000	402265	580911	430269	366639	355736	102877	269702	146209	475314	348762	274997	1330834
PE(O-16:1_20:5)	32451	18578	37061	20165	38049	11432	27152	27712	21914	38400	33396	12949	42801	24356	64210	81519	39279	207346
PE(O-16:1_22:4)	1238671	611565	1301352	405931	1237564	272496	709872	1063517	569729	987344	1095883	170001	919105	447538	1906689	551256	445936	1969031
PE(O-17:1_20:4)	49175	25097	50623	17070	40273	11551	27068	34936	23832	26623	26856	6047	45585	24198	85947	22873	15281	91368
PE(O-18:1_20:4)	77464	38628	69215	19626	54777	12592	29767	55864	27376	32549	65177	8862	42878	11591	75339	27640	11503	91666
PE(14:0_20:4)	47954	41425	31657	30743	42505	33381	41682	34522	33713	47554	28680	18327	19141	10393	19677	10531	12712	19772
PE(O-18:1_22:3)	86649	34089	76264	8551	60360	6231	21794	33196	11498	68042	74766	4531	55484	24922	68000	11657	7039	28469
PE(18:0_22:3)	10637	4220	10768	2176	6165	2825	2533	4693	2512	16518	23831	1186	6405	2669	16807	541	262	2429
PE(22:3_20:4)	16820	2668	15549	1985	12896	2195	14690	18551	2389	64525	57991	1826	3193	5109	25538	2902	2471	4506
PE(18:0_22:4)	153679	62669	149389	30229	77935	16216	69322	60530	49033	131123	115956	9198	35084	14433	78809	14769	12686	76733
PE(8:0_16:1)	20380	17534	11383	12119	17813	13194	28429	23985	25662	15731	17256	16220	24826	21249	24347	1451	1317	1437
PE(O-15:1_20:4)	4022	2816	3433	3715	4868	2456	3630	5769	4442	3430	2765	1025	10567	6530	17644	9765	3344	34473
PE(O-17:1_18:1)	1834	614	2139	319	1443	232	384	934	511	2139	3105	114	2558	1052	6483	872	571	6265
PE(O-18:1_18:1)	16893	6506	15790	2013	10345	1436	4435	7635	3293	11120	23595	1680	7456	4059	21684	5792	4085	45977
PE(16:0_20:5)	194019	150775	140737	115243	215294	116449	205636	184776	156447	219651	196472	89653	172407	101987	206820	95882	81478	117111
PE(16:0_16:0)	3541	2585	3103	2279	4473	1620	2451	2136	2768	1607	1508	1140	968	501	2349	1079	1248	2746
PE(17:0_20:4)	71570	31728	56122	25194	69296	21588	52006	45395	36493	60379	40507	13670	58818	20944	98371	19128	12232	31154
PE(18:2_20:4)	557315	355789	361273	266503	597767	117308	544821	469887	409158	529302	409778	80189	196424	110839	212	582003	263642	724449
PE(O-17:1_22:4)	30481	14439	25908	8467	26561	7181	13823	20227	13749	21804	25524	4470	12022	39129	29758	11981	6910	40640
PE(18:0_20:3)	117770	49298	166832	27253	58355	10655	26615	44574	37206	192760	195710	21618	88458	58698	221485	17982	25847	23700
PE(18:1_20:1)	10247	9948	7307	8076	7016	3776	13790	12122	8068	3899	5499	3045	9900	8522	9620	29157	27093	31720
PE(O-18:0_22:5)	541511	277783	478610	109359	397483	39618	127881	296919	131866	334739	357273	35010	136560	45994	259329	79769	71057	284374
PE(17:0_22:4)	14957	3358	178	3574	10050	2735	2356	6597	2082	5853	6041	3498	5904	2908	12567	1209	657	11687
PE(18:1_22:6)	207652	125728	153287	107120	269606	118941	267477	255406	170550	347928	365775	99551	286137	169224	399571	182714	169604	278167
PE(20:2_20:4)	333016	175957	264589	149869	334168	121146	265764	295275	202482	298576	263576	75282	172358	99398	229599	109811	117492	204501
PE(18:1_22:4)	295526	114435	254494	103573	195634	67454	173081	185137	155685	198878	188780	40063	65575	34438	155223	78210	88410	233196
PE(16:0_16:1)	89033	40622	85619	26405	85264	25808	49482	57706	47324	64571	55616	17368	37110	17325	63770	15488	16974	29862
PE(16:0_18:3)	71025	37059	63944	34485	71575	25355	49599	52075	51602	64994	51190	17989	58846	30957	95076	41599	46643	53197
PE(17:0_18:2)	4855	2544	4510	685	4605	1244	2941	3922	3416	4885	3010	1625	9939	6417	18701	4339	4779	9084
PE(15:0_20:4)	28019	18859	18634	15729	25679	11664	19041	23235	16707	26459	20519	8926	25362	12219	34936	8585	7749	19209
PE(O-18:2_18:1)	79775	42685	71844	16818	85644	17284	34377	51630	29845	53814	63114	10474	35055	20572	78410	34773	35465	159387
PE(17:1_20:4)	25282	12596	16313	8792	22329	7175	19851	17368	18612	16395	18331	5242	29914	12860	39711	4455	4260	12512
PE(O-18:2_22:4)	684293	384582	598169	212750	491514	133220	258299	361727	214267	377488	315130	37541	91143	49115	220786	153433	145639	477527
PE(18:1_18:2)	224370	101053	326549	120131	320005	62851	174925	258031	156895	278685	295555	75506	302448	201501	460564	466614	793119	625685
PE(15:0_18:1)	8831	4047	7258	2494	7355	1923	3546	5207	3793	6502	8107	2032	7090	4314	12997	4282	3094	9265
PE(O-17:1_22:6)	6212	3692	6338	3497	5753	2931	3113	8175	3808	4728	5667	733	4109	2629	7195	8398	2672	20002
PE(O-18:3_20:4)	161083	123523	158960	103335	183360	77709	132627	177821	114879	152587	144097	47519	96194	57696	126996	245476	124686	474779
PE(16:2_20:4)	20873	25839	12883	24901	17170	20260	20528	17998	18785	34650	28986	16996	17858	12702	23236	2005	1439	3975
PE(O-18:2_22:6)	156757	110468	109495	81010	139898	66574	76511	131160	88224	77516	87048	20376	31044	20486	43256	109012	73572	222361
PG(18:2_18:2)	21358	21321	22857	20038	26351	18367	33270	31453	20620	22818	27763	13034	55706	45149	57458	2150	2428	2370
PG(18:2_22:5)	51161	68305	26705	50276	20094	26564	26744	45915	26017	29335	73791	38470	37989	46924	50567	895	89	90
PG(20:4_20:4)	13794	22476	5698	14087	4740	10003	7591	13196	7745	17559	39105	27380	20209	19481	25101	98	1	1
PG(16:0_18:1)	21977	12509	25885	10808	29765	8733	38810	29117	15611	50979	31037	5323	23891	10155	35413	602	808	3060
PG(18:2_20:3)	38725	44576	32502	35487	27832	25242	36649	51359	27020	32359	65059	26613	68381	79079	79536	422	430	1590
PG(18:1_22:4)	44663	45671	30900	24326	24564	11515	24521	44350	26635	25764	55257	13815	19996	20853	43134	1	1	1
PG(22:5_22:5)	41323	71897	10721	41576	7045	14102	11582	18282	16801	25536	34954	28763	1446	1005	5237	286	313	706
PG(18:2_20:4)	16586	23857	10276	17696	9642	12929	8283	18943	10839	18024	32315	19185	26351	26596	33443	84	106	484
PG(18:1_20:3)	13463	14717	14740	12886	5981	6653	8590	26610	10056	34526	17690	6260	12040	32330	18666	1	475	2231
PG(18:2_22:4)	72226	48583	38967	54354	30646	28870	35473	55831	38172	32045	77041	32688	31422	48392	51842	1	32	223
PG(20:3_22:4)	30944	34807	16992	26462	11891	9201	13945	31152	10519	22348	62706	23171	9865	13233	25698	2963	2873	1524
PG(18:1_18:2)	16171	36695	36307	29073	38006	24679	50925	50852	34312	34257	37172	14244	57129	55227	72942	6112	2098	7921
PG(22:4_22:5)	55589	68152	16862	41210	11529	12773	14244	24977	18269	34375	54995	29534	1369	700	2419	584	922	489
PG(20:3_22:5)	43372	62438	20888	44927	12444	17459	18055	34287	17995	37245	92365	50032	11018	10249	22861	216	258	321
PI(16:0_18:2)	11772	65693	58751	48665	89621	54089	57400	81868	56454	76068	85126	32983	65228	62019	92928	85795	70391	114962
PI(16:0_20:4)	28906	22887	23644	16990	27616	18764	27012	27589	19584	29256	27156	8151	14784	9378	20319	57697	34582	117443
PI(18:0_18:2)	57608	36184	38218	23646	55658	31140	43350	42602	24298	135936	138036	44464	55811	31882	71209	15107	11158	43411
PI(18:0_20:3)	77167	25364	101576	10139	50810	10045	28628	46816	18935	119963	129509	16146	64692	28841	128549	5725	4055	21085
PI(18:1_20:4)	38864	21587	36322	16048	40872	14286	41951	30164	28910	27920	22445	8044	20948	13902	30097	28549	20894	66155
PI(16:0_18:0)	511327	256855	379019	146682	287726	102691	213984	283461	170703	159156	225688	33668	80384	48250	120158	110603	113366	339969
PI(26:1_14:2)	362676	301357	300316	363918	340447	332599	538232	460984	461400	491058	482352	325262	422776	354421	495551	553134	486905	575647
PI(18:0_20:4)	699903	278033	563123	123852	521043	89535	425874	469049	256692	535735	433111	51981	233795	108707	514590	94738	47887	419503
PI(22:4_18:0)	31350	15082	22640	8932	13007	6099	15496	15865	10497	105	27541	8005	3709	3047	7783	3371	1983	14499
PS(20:4_22:4)	42770	34065	17264	21207	36224	31629	37025	34814	22689	75976	57925	28141	19569	12914	35612	8742	8399	18035
PS(16:0_20:4)	48220	30508	16115	20158	54552	25365	30771	23626	22786	37485	26929	8961	26356	13302	22586	34049	33167	55549
PS(O-18:0_20:4)	32820	48488	55131	38826	57484	40802	53048	56748	42447	45641	44757	24356	56855	35962	59005	32951	36395	41993
PS(18:2_20:4)	29298	21327	25217	16542	37382	24031	29485	29591	22014	22225	18776	9916	16982	27979	35855	41138	37436	53323
PS(18:0_21:4)	97474	44613	73995	30888	79061	22351	51308	71109	46944	57666	62322	16674	68812	34994	99719	19212	13641	41913
PS(16:0_18:1)	35838	5861	44936	4365	41401	709	16860	23933	13281	14441	13278	1780	13949	4503	16378	495	847	25502
PS(18:0_22:6)	463095	280357	323894	168276	405121	150257	289142	353944	225376	280403	294979	64473	165660	140583	211723	481667	238571	754052
PS(20:3_20:4)	172696	130649	58080	101473	168026	98022	162421	163036	114791	154873	171506	65156	169985	128190	183816	128956	113873	169587
PS(22:3_20:4)	80782	67355	56050	40353	86960	54495	68820	67586	40300	242143	236477	78164	92705	53768	117608	7859	7526	9558
PS(24:3_20:4)	93387	58204	46082	35484	68247	29097	71670	66049	37691	186911	161294	51791	81202	44926	70056	20525	18856	46665
PS(18:0_22:4)	874211	254019	696895	246622	615864	193960	475001	600628	366690	243121	480573	179783	286203	195753	377914	212162	46644	822521
PS(20:3_22:6)	11983	11066	9592	8427	11492	6177	9398	10514	6934	7988	8307	3786	5324	4156	4416	18592	11200	22884
PS(17:0_20:4)	91049	44315	54185	27939	83986	29613	72685	72243	44316	39469	33860	9214	89589	48767	107699	9295	9575	15740
SM(d35:1)	1813084	1015822	1511245	733609	1650264	718501	1464202	1645792	1069423	2631790	2205365	574625	1560461	940819	2372118	1057765	710799	2343245
SM(d41:2)	81835	39305	76688	19007	73372	12874	47986	77335	40131	165520	146662	12381	43673	16032	96619	44604	20091	166596
SM(d41:3)	33334	16386	27056	16295	22468	15590	16881	31252	23583	29041	25719	5968	8461	5183	11154	9861	5930	18920
SM(d33:0)	10945	8872	7588	5038	9714	5453	7629	9661	9681	17342	12810	6437	19071	14899	26759	7083	4234	12372
SM(d43:4)	115522	82310	95485	63678	106542	56405	94160	116659	78935	136143	111804	30942	21239	13167	35799	21872	15181	43702
SM(d43:3)	355617	177483	352733	103606	300973	60260	185700	295988	143386	385759	347943	41088	61289	26536	128268	124754	75652	394452
SM(d37:2)	156838	97427	115601	68664	144628	67297	114651	120823	95340	131707	142976	56062	168831	123105	221979	105389	102059	224683
MS_METABOLITE_DATA_END
#METABOLITES
METABOLITES_START
metabolite_name	Lipid Class	Polarity	m/z	RT
Cer(d18:2_17:0)	Cer	5MM-	594.510	3.368
FA(27:0)	FA	5MM-	409.405	3.325
FA(15:0)	FA	5MM-	241.217	2.567
FA(26:0)	FA	5MM-	425.364	3.108
FA(29:0)	FA	5MM-	437.436	3.637
FA(30:0)	FA	5MM-	451.452	3.480
FA(28:0)	FA	5MM-	453.395	3.331
FA(16:1)	FA	5MM-	253.217	2.509
FA(19:0)	FA	5MM-	297.280	2.997
FA(18:3)	FA	5MM-	277.217	2.432
FA(18:2)	FA	5MM-	279.233	2.201
FA(20:5)	FA	5MM-	301.217	2.616
FA(20:4)	FA	5MM-	303.233	2.203
FA(20:3)	FA	5MM-	305.249	2.668
FA(22:6)	FA	5MM-	327.233	2.498
FA(22:5)	FA	5MM-	329.249	2.633
FA(22:4)	FA	5MM-	331.264	2.749
FA(24:0)	FA	5MM-	397.332	2.984
FA(28:7)	FA	5MM-	409.311	3.175
FA(20:2)	FA	5MM-	307.264	2.809
FA(22:0)	FA	5MM-	369.301	2.800
FA(20:1)	FA	5MM-	309.280	2.940
FA(15:1)	FA	5MM-	239.202	2.359
FA(17:1)	FA	5MM-	267.233	2.643
FA(22:3)	FA	5MM-	333.280	2.886
FA(24:1)	FA	5MM-	365.343	3.257
FA(22:1)	FA	5MM-	337.311	3.109
FA(24:2)	FA	5MM-	363.327	3.150
FA(16:3)	FA	5MM-	249.186	0.988
FA(18:0)	FA	5MM-	283.264	2.856
FA(17:0)	FA	5MM-	269.249	2.788
FA(20:0)	FA	5MM-	311.296	3.092
FA(23:0)	FA	5MM-	353.343	3.331
FA(18:1)	FA	5MM-	281.249	2.367
FA(14:0)	FA	5MM-	227.202	2.427
FA(12:0)	FA	5MM-	199.170	2.103
FA(21:0)	FA	5MM-	325.311	2.980
FA(25:0)	FA	5MM-	381.374	3.438
LPC(O-19:1)	LPC	5MM-	566.383	2.513
LPC(O-19:0)	LPC	5MM-	568.398	2.690
LPC(19:2)	LPC	5MM-	578.346	2.267
LPC(19:1)	LPC	5MM-	580.362	2.428
LPE(16:0)	LPE	5MM-	452.278	2.406
LPE(18:0)	LPE	5MM-	480.310	2.632
LPE(18:1)	LPE	5MM-	478.294	2.423
LPE(20:3)	LPE	5MM-	502.294	2.408
LPE(20:4)	LPE	5MM-	500.278	2.260
LPE(22:3)	LPE	5MM-	530.325	2.582
LPE(22:4)	LPE	5MM-	528.310	2.445
LPE(22:5)	LPE	5MM-	526.294	2.304
LPE(P-18:1)	LPE	5MM-	462.299	2.556
LPE(P-18:0)	LPE	5MM-	464.315	2.730
LPG(20:3)	LPG	5MM-	533.288	2.165
LPG(22:4)	LPG	5MM-	559.304	2.266
LPG(22:5)	LPG	5MM-	557.288	2.223
LPG(18:1)	LPG	5MM-	509.288	2.237
LPG(20:4)	LPG	5MM-	531.273	2.084
LPI(18:0)	LPI	5MM-	599.320	2.396
LPI(20:3)	LPI	5MM-	621.305	2.214
LPS(18:0)	LPS	5MM-	524.299	2.398
PA(16:0_18:2)	PA	5MM-	671.466	3.207
PA(16:0_20:4)	PA	5MM-	695.466	3.200
PC(O-14:1_22:4)	PC	5MM-	750.544	3.477
PC(O-19:2_20:4)	PC	5MM-	850.597	3.378
PC(16:0_18:2)	PC	5MM-	742.539	3.317
PC(16:0_17:0)	PC	5MM-	792.576	3.378
PC(17:0_18:2)	PC	5MM-	816.576	3.331
PC(O-16:1_22:4)	PC	5MM-	778.576	3.539
PC(O-19:1_20:4)	PC	5MM-	852.612	3.403
PC(19:0_22:6)	PC	5MM-	892.607	3.364
PC(16:0_20:4)	PC	5MM-	766.539	3.317
PC(O-19:0_18:1)	PC	5MM-	832.644	3.541
PC(O-14:1_18:2)	PC	5MM-	698.513	3.416
PC(O-14:1_18:1)	PC	5MM-	700.529	3.472
PC(O-16:1_18:1)	PC	5MM-	728.560	3.546
PC(16:0_18:1)	PC	5MM-	744.555	3.247
PC(O-17:0_18:1)	PC	5MM-	804.612	3.452
PC(17:0_18:1)	PC	5MM-	818.592	3.389
PC(18:0_18:2)	PC	5MM-	830.592	3.375
PC(9:0_18:0)	PC	5MM-	708.482	3.043
PC(O-14:1_20:4)	PC	5MM-	722.513	3.404
PC(17:0_16:1)	PC	5MM-	790.560	3.300
PC(18:2_19:2)	PC	5MM-	840.576	3.310
PC(O-19:2_18:1)	PC	5MM-	828.612	3.460
PC(17:0_20:3)	PC	5MM-	842.592	3.361
PC(18:0_20:4)	PC	5MM-	854.592	3.372
PC(19:2_20:4)	PC	5MM-	864.576	3.262
PC(19:0_22:4)	PC	5MM-	896.639	3.482
PC(O-16:1_22:3)	PC	5MM-	780.591	3.548
PC(18:0_18:1)	PC	5MM-	832.607	3.441
PC(17:0_20:5)	PC	5MM-	838.560	3.288
PC(19:0_18:1)	PC	5MM-	846.623	3.485
PC(21:4_20:4)	PC	5MM-	888.576	3.252
PC(16:0_20:3)	PC	5MM-	768.555	3.355
PC(18:1_18:2)	PC	5MM-	768.555	3.494
PC(O-17:0_20:4)	PC	5MM-	826.597	3.384
PC(O-19:1_18:1)	PC	5MM-	830.628	3.477
PC(17:0_22:4)	PC	5MM-	868.607	3.413
PC(O-17:0_18:2)	PC	5MM-	802.597	3.405
PC(O-19:0_20:4)	PC	5MM-	854.628	3.481
PC(O-17:1_18:2)	PC	5MM-	800.581	3.373
PC(O-16:2_22:6)	PC	5MM-	772.529	3.395
PC(O-17:1_20:4)	PC	5MM-	824.581	3.361
PC(18:1_19:1)	PC	5MM-	844.607	3.420
PC(19:1_20:4)	PC	5MM-	866.592	3.340
PC(6:0_16:1)	PC	5MM-	576.367	2.791
PC(O-16:2_22:4)	PC	5MM-	776.560	3.496
PC(17:1_18:2)	PC	5MM-	814.560	3.258
PC(13:1_18:1)	PC	5MM-	760.513	3.211
PE(16:0_18:1)	PE	5MM-	716.524	3.424
PE(16:0_18:2)	PE	5MM-	714.508	3.350
PE(16:0_20:4)	PE	5MM-	738.508	3.348
PE(18:0_18:1)	PE	5MM-	744.555	3.509
PE(18:0_20:4)	PE	5MM-	766.539	3.437
PE(18:1_18:1)	PE	5MM-	742.539	3.444
PE(18:1_20:4)	PE	5MM-	764.524	3.365
PE(O-16:1_18:1)	PE	5MM-	700.529	3.478
PE(O-16:1_18:2)	PE	5MM-	698.513	3.412
PE(O-16:1_20:3)	PE	5MM-	724.529	3.425
PE(O-16:1_20:4)	PE	5MM-	722.513	3.401
PE(O-16:1_20:5)	PE	5MM-	720.497	3.340
PE(O-16:1_22:4)	PE	5MM-	750.544	3.415
PE(O-17:1_20:4)	PE	5MM-	736.529	3.448
PE(O-18:1_20:4)	PE	5MM-	750.544	3.569
PE(14:0_20:4)	PE	5MM-	710.477	3.238
PE(O-18:1_22:3)	PE	5MM-	780.591	3.594
PE(18:0_22:3)	PE	5MM-	796.586	3.546
PE(22:3_20:4)	PE	5MM-	816.555	3.164
PE(18:0_22:4)	PE	5MM-	794.571	3.494
PE(8:0_16:1)	PE	5MM-	576.367	2.790
PE(O-15:1_20:4)	PE	5MM-	708.497	3.334
PE(O-17:1_18:1)	PE	5MM-	714.544	3.506
PE(O-18:1_18:1)	PE	5MM-	728.560	3.550
PE(16:0_20:5)	PE	5MM-	736.492	3.275
PE(16:0_16:0)	PE	5MM-	690.508	3.276
PE(17:0_20:4)	PE	5MM-	752.524	3.391
PE(18:2_20:4)	PE	5MM-	762.508	3.310
PE(O-17:1_22:4)	PE	5MM-	764.560	3.502
PE(18:0_20:3)	PE	5MM-	768.555	3.479
PE(18:1_20:1)	PE	5MM-	770.571	3.422
PE(O-18:0_22:5)	PE	5MM-	778.576	3.543
PE(17:0_22:4)	PE	5MM-	780.555	3.360
PE(18:1_22:6)	PE	5MM-	788.524	3.326
PE(20:2_20:4)	PE	5MM-	790.539	3.394
PE(18:1_22:4)	PE	5MM-	792.555	3.435
PE(16:0_16:1)	PE	5MM-	688.492	3.331
PE(16:0_18:3)	PE	5MM-	712.492	3.295
PE(17:0_18:2)	PE	5MM-	728.524	3.390
PE(15:0_20:4)	PE	5MM-	724.492	3.302
PE(O-18:2_18:1)	PE	5MM-	726.544	3.485
PE(17:1_20:4)	PE	5MM-	750.508	3.310
PE(O-18:2_22:4)	PE	5MM-	776.560	3.496
PE(18:1_18:2)	PE	5MM-	740.524	3.372
PE(15:0_18:1)	PE	5MM-	702.508	3.373
PE(O-17:1_22:6)	PE	5MM-	760.529	3.418
PE(O-18:3_20:4)	PE	5MM-	746.513	3.375
PE(16:2_20:4)	PE	5MM-	734.477	3.231
PE(O-18:2_22:6)	PE	5MM-	772.529	3.398
PG(18:2_18:2)	PG	5MM-	769.503	3.103
PG(18:2_22:5)	PG	5MM-	819.518	3.078
PG(20:4_20:4)	PG	5MM-	817.503	3.018
PG(16:0_18:1)	PG	5MM-	747.518	3.211
PG(18:2_20:3)	PG	5MM-	795.518	3.096
PG(18:1_22:4)	PG	5MM-	823.549	3.174
PG(22:5_22:5)	PG	5MM-	869.534	2.852
PG(18:2_20:4)	PG	5MM-	793.503	3.029
PG(18:1_20:3)	PG	5MM-	797.534	3.145
PG(18:2_22:4)	PG	5MM-	821.534	3.146
PG(20:3_22:4)	PG	5MM-	847.549	3.165
PG(18:1_18:2)	PG	5MM-	771.518	3.124
PG(22:4_22:5)	PG	5MM-	871.549	3.150
PG(20:3_22:5)	PG	5MM-	845.534	3.107
PI(16:0_18:2)	PI	5MM-	833.519	3.116
PI(16:0_20:4)	PI	5MM-	857.519	3.110
PI(18:0_18:2)	PI	5MM-	861.550	3.216
PI(18:0_20:3)	PI	5MM-	887.566	3.252
PI(18:1_20:4)	PI	5MM-	883.534	3.135
PI(16:0_18:0)	PI	5MM-	837.550	3.256
PI(26:1_14:2)	PI	5MM-	915.597	3.098
PI(18:0_20:4)	PI	5MM-	885.550	3.215
PI(22:4_18:0)	PI	5MM-	913.581	3.278
PS(20:4_22:4)	PS	5MM-	858.529	3.133
PS(16:0_20:4)	PS	5MM-	782.498	3.117
PS(O-18:0_20:4)	PS	5MM-	796.550	3.286
PS(18:2_20:4)	PS	5MM-	806.498	3.066
PS(18:0_21:4)	PS	5MM-	824.545	3.233
PS(16:0_18:1)	PS	5MM-	760.513	3.207
PS(18:0_22:6)	PS	5MM-	834.529	3.207
PS(20:3_20:4)	PS	5MM-	832.513	3.102
PS(22:3_20:4)	PS	5MM-	860.545	3.198
PS(24:3_20:4)	PS	5MM-	888.576	3.261
PS(18:0_22:4)	PS	5MM-	838.560	3.285
PS(20:3_22:6)	PS	5MM-	856.513	3.198
PS(17:0_20:4)	PS	5MM-	796.513	3.170
SM(d35:1)	SM	5MM-	761.581	3.272
SM(d41:2)	SM	5MM-	843.660	3.488
SM(d41:3)	SM	5MM-	841.644	3.425
SM(d33:0)	SM	5MM-	735.566	3.191
SM(d43:4)	SM	5MM-	867.660	3.444
SM(d43:3)	SM	5MM-	869.675	3.501
SM(d37:2)	SM	5MM-	787.597	3.306
METABOLITES_END
#END