#METABOLOMICS WORKBENCH nsouthwell_20240306_145021 DATATRACK_ID:4690 STUDY_ID:ST003127 ANALYSIS_ID:AN005127 VERSION 1 CREATED_ON 03-22-2024 #PROJECT PR:PROJECT_TITLE High fat diet ameliorates mitochondrial cardiomyopathy in CHCHD10 mutant mice PR:PROJECT_SUMMARY Mutations in CHCHD10, a mitochondrial protein with undefined functions, are PR:PROJECT_SUMMARY associated with autosomal dominant mitochondrial diseases. Chchd10 knock-in mice PR:PROJECT_SUMMARY harboring a heterozygous S55L mutation (equivalent to human pathogenic S59L) PR:PROJECT_SUMMARY develop a fatal mitochondrial cardiomyopathy caused by CHCHD10 aggregation and PR:PROJECT_SUMMARY proteotoxic mitochondrial integrated stress response (mtISR). In mutant hearts, PR:PROJECT_SUMMARY mtISR is accompanied by a metabolic rewiring characterized by increased reliance PR:PROJECT_SUMMARY on glycolysis rather than fatty acid oxidation. To counteract this metabolic PR:PROJECT_SUMMARY rewiring, heterozygous S55L mice were subjected to chronic high fat diet (HFD) PR:PROJECT_SUMMARY to decrease insulin sensitivity and glucose uptake and enhance fatty acid PR:PROJECT_SUMMARY utilization in the heart. HFD ameliorated the ventricular dysfunction of mutant PR:PROJECT_SUMMARY hearts and significantly extended the survival of mutant female mice affected by PR:PROJECT_SUMMARY severe pregnancy-induced cardiomyopathy. Gene expression profiles confirmed that PR:PROJECT_SUMMARY HFD increased fatty acid utilization and ameliorated cardiomyopathy markers. PR:PROJECT_SUMMARY Importantly, HFD also decreased accumulation of aggregated CHCHD10 in the S55L PR:PROJECT_SUMMARY heart, suggesting activation of quality control mechanisms. Overall, our PR:PROJECT_SUMMARY findings indicate that metabolic therapy can be effective in mitochondrial PR:PROJECT_SUMMARY cardiomyopathies associated with proteotoxic stress. PR:INSTITUTE Weill Cornell Medicine PR:LAST_NAME Southwell PR:FIRST_NAME Nneka PR:ADDRESS 407 E 61st St PR:EMAIL nns4001@med.cornell.edu PR:PHONE 646-962-8172 PR:DOI http://dx.doi.org/10.21228/M8FD9G #STUDY ST:STUDY_TITLE Effect of high fat diet on heart lipidome in CHCHD10 mutant mice ST:STUDY_SUMMARY Mutations in CHCHD10, a mitochondrial protein with undefined functions, are ST:STUDY_SUMMARY associated with autosomal dominant mitochondrial diseases. Chchd10 knock-in mice ST:STUDY_SUMMARY harboring a heterozygous S55L mutation (equivalent to human pathogenic S59L) ST:STUDY_SUMMARY develop a fatal mitochondrial cardiomyopathy caused by CHCHD10 aggregation and ST:STUDY_SUMMARY proteotoxic mitochondrial integrated stress response (mtISR). In mutant hearts, ST:STUDY_SUMMARY mtISR is accompanied by a metabolic rewiring characterized by increased reliance ST:STUDY_SUMMARY on glycolysis rather than fatty acid oxidation. To counteract this metabolic ST:STUDY_SUMMARY rewiring, heterozygous S55L mice were subjected to chronic high fat diet (HFD) ST:STUDY_SUMMARY to decrease insulin sensitivity and glucose uptake and enhance fatty acid ST:STUDY_SUMMARY utilization in the heart. HFD ameliorated the ventricular dysfunction of mutant ST:STUDY_SUMMARY hearts and significantly extended the survival of mutant female mice affected by ST:STUDY_SUMMARY severe pregnancy-induced cardiomyopathy. Gene expression profiles confirmed that ST:STUDY_SUMMARY HFD increased fatty acid utilization and ameliorated cardiomyopathy markers. ST:STUDY_SUMMARY Importantly, HFD also decreased accumulation of aggregated CHCHD10 in the S55L ST:STUDY_SUMMARY heart, suggesting activation of quality control mechanisms. Overall, our ST:STUDY_SUMMARY findings indicate that metabolic therapy can be effective in mitochondrial ST:STUDY_SUMMARY cardiomyopathies associated with proteotoxic stress. ST:INSTITUTE Weill Cornell Medicine ST:LAST_NAME Southwell ST:FIRST_NAME Nneka ST:ADDRESS 407 E 61st St ST:EMAIL nns4001@med.cornell.edu ST:PHONE 646-962-8172 ST:SUBMIT_DATE 2024-03-06 #SUBJECT SU:SUBJECT_TYPE Mammal SU:SUBJECT_SPECIES Mus musculus SU:TAXONOMY_ID 10090 #SUBJECT_SAMPLE_FACTORS: SUBJECT(optional)[tab]SAMPLE[tab]FACTORS(NAME:VALUE pairs separated by |)[tab]Additional sample data SUBJECT_SAMPLE_FACTORS - HL_HetCD_1 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_1.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_2 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_2.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_3 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_3.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_5 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_5.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_10 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_10.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_6 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_6.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_7 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_7.raw SUBJECT_SAMPLE_FACTORS - HL_HetCD_8 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetCD_8.raw SUBJECT_SAMPLE_FACTORS - HetCD_mixneg Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HetCD_mixneg.raw SUBJECT_SAMPLE_FACTORS - HetCD_mixpos Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HetCD_mixpos.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_2 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_2.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_3 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_3.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_4 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_4.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_5 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_5.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_10 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_10.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_6 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_6.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_7 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_7.raw SUBJECT_SAMPLE_FACTORS - HL_HetHFD_9 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_HetHFD_9.raw SUBJECT_SAMPLE_FACTORS - HetHFD_mixneg Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HetHFD_mixneg.raw SUBJECT_SAMPLE_FACTORS - HetHFD_mixpos Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HetHFD_mixpos.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_1 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_1.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_2 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_2.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_3 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_3.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_4 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_4.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_10 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_10.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_6 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_6.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_8 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_8.raw SUBJECT_SAMPLE_FACTORS - HL_WTCD_9 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTCD_9.raw SUBJECT_SAMPLE_FACTORS - WTCD_mixneg Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=WTCD_mixneg.raw SUBJECT_SAMPLE_FACTORS - WTCD_mixpos Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=WTCD_mixpos.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_1 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_1.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_3 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_3.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_4 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_4.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_5 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_5.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_10 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_10.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_6 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_6.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_7 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_7.raw SUBJECT_SAMPLE_FACTORS - HL_WTHFD_8 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=HL_WTHFD_8.raw SUBJECT_SAMPLE_FACTORS - WTHFD_mixneg Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=WTHFD_mixneg.raw SUBJECT_SAMPLE_FACTORS - WTHFD_mixpos Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:N/A | Age:75 RAW_FILE_NAME(Named Raw File_Heart Lipidomics)=WTHFD_mixpos.raw #COLLECTION CO:COLLECTION_SUMMARY Murine heart tissue was excised and snap frozen in liquid N2. CO:SAMPLE_TYPE Heart #TREATMENT TR:TREATMENT_SUMMARY CHCHD10 WT and CHCHD10 S55L heterozygous mice were treated with either a control TR:TREATMENT_SUMMARY diet (70% Carbohydrate, 20% Protein, 10% Fat) or High Fat Diet (60% Fat, 20% TR:TREATMENT_SUMMARY Protein, 20% Carbohydrate) in utero until 75 days of age. #SAMPLEPREP SP:SAMPLEPREP_SUMMARY In brief, 1 ml of 90% isopropanol was added to 15 mg of homogenized heart SP:SAMPLEPREP_SUMMARY tissue. Samples were vortexed for 2 min, sonicated for 1 min using a probe SP:SAMPLEPREP_SUMMARY sonicator and centrifuged at 15,000 g for 10 min. The supernatant was collected SP:SAMPLEPREP_SUMMARY and dried down using a SpeedVac. The dried samples were reconstituted using SP:SAMPLEPREP_SUMMARY acetonitrile/isopropanol/water 65:30:5 containing stable isotope labeled SP:SAMPLEPREP_SUMMARY internal lipid standards (Splash Lipidomix, Avanti Polar Lipids) prior to LC-MS SP:SAMPLEPREP_SUMMARY analysis. #CHROMATOGRAPHY CH:METHODS_FILENAME Method_Lipidomics.pdf CH:INSTRUMENT_NAME Thermo Vanquish CH:COLUMN_NAME Imtakt Cadenza CD-C18 (150 x 2.1mm, 3um) CH:COLUMN_TEMPERATURE 35 CH:FLOW_GRADIENT 0–1.5 min, 32% B; 1.5-4 min, 32-45% B; 4-5 min, 45-52% B; 5-8 min, 52-58% B; CH:FLOW_GRADIENT 8-11 min, 58-66% B; 11-14 min, 66-70% B; 14-18 min, 70-75% B; 21-25 min, CH:FLOW_GRADIENT isocratic 97% B, 25-25.1 min 97-32% B CH:FLOW_RATE 200 µl/min CH:SOLVENT_A 60% acetonitrile/40% water; 10 mM ammonium formate; 0.1% formic acid CH:SOLVENT_B 90% isopropanol/10% acetonitrile; 10 mM ammonium formate; 0.1% formic acid CH:CHROMATOGRAPHY_TYPE Reversed phase #ANALYSIS AN:ANALYSIS_TYPE MS AN:ANALYSIS_PROTOCOL_FILE Method_Lipidomics.pdf #MS MS:INSTRUMENT_NAME Thermo Q Exactive Orbitrap MS:INSTRUMENT_TYPE Orbitrap MS:MS_TYPE ESI MS:MS_COMMENTS A data-dependent mass spectrometric acquisition method was used for lipid MS:MS_COMMENTS identification. In this method, each MS survey scan was followed by up to 10 MS:MS_COMMENTS MS/MS scans performed on the most abundant ions. Data was acquired in positive MS:MS_COMMENTS and negative mode in separate runs. The following electrospray parameters were MS:MS_COMMENTS used: spray voltage 3.0 kV, heated capillary temperature 350 °C, HESI probe MS:MS_COMMENTS temperature 350 °C, sheath gas, 35 units; auxiliary gas 10 units. For MS MS:MS_COMMENTS scans: resolution, 70,000 (at m/z 200); automatic gain control target, 3e6; MS:MS_COMMENTS maximum injection time, 200 ms; scan range, 250-1800 m/z. For MS/MS scans: MS:MS_COMMENTS resolution, 17,500 (at 200 m/z); automatic gain control target, 1e5 ions; MS:MS_COMMENTS maximum injection time, 75 ms; isolation window, 1 m/z; NCE, stepped 20,30 and MS:MS_COMMENTS 40. The LC-MS results were processed using MS-DIAL software (version 4.9) for MS:MS_COMMENTS lipid identification and relative quantitation. MS:ION_MODE NEGATIVE #MS_METABOLITE_DATA MS_METABOLITE_DATA:UNITS Peak Intensity MS_METABOLITE_DATA_START Samples HL_HetCD_1 HL_HetCD_2 HL_HetCD_3 HL_HetCD_5 HL_HetCD_10 HL_HetCD_6 HL_HetCD_7 HL_HetCD_8 HL_HetHFD_2 HL_HetHFD_3 HL_HetHFD_4 HL_HetHFD_5 HL_HetHFD_10 HL_HetHFD_6 HL_HetHFD_7 HL_HetHFD_9 HL_WTCD_1 HL_WTCD_2 HL_WTCD_3 HL_WTCD_4 HL_WTCD_10 HL_WTCD_6 HL_WTCD_8 HL_WTCD_9 HL_WTHFD_1 HL_WTHFD_3 HL_WTHFD_4 HL_WTHFD_5 HL_WTHFD_10 HL_WTHFD_6 HL_WTHFD_7 HL_WTHFD_8 Factors Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:CHCHD10 S55L Het | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:Control Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:F | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 Sample source:Heart | Genotype:WT | Treatment:High Fat Diet | Sex:M | Age:75 CL 71:4 249820.0000 229648.9000 203268.5000 199999.4000 207130.0000 284321.2000 199918.2000 179238.3000 120774.5000 163383.0000 165138.0000 210518.3000 161388.9000 218277.3000 189829.4000 172392.6000 176349.5000 100835.7000 228270.2000 117705.5000 123938.6000 85445.8200 111329.0000 100760.2000 213472.7000 99127.3400 166941.7000 194239.8000 137698.1000 171400.6000 162149.9000 132275.4000 CL 72:5 20448060.0000 17578310.0000 22884280.0000 17601180.0000 16699030.0000 19708870.0000 23287500.0000 16832500.0000 11741630.0000 15532100.0000 12219370.0000 15416590.0000 13724950.0000 13050730.0000 13321830.0000 11182710.0000 16075250.0000 12675350.0000 19109450.0000 16532860.0000 12325820.0000 12045130.0000 14539330.0000 12557260.0000 18498370.0000 10769700.0000 13761630.0000 13636890.0000 11437140.0000 13801110.0000 12873220.0000 11745060.0000 CL 72:6 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6303783.0000 2695686.0000 4354496.0000 5074190.0000 4870677.0000 4191415.0000 6751966.0000 4245954.0000 3373369.0000 4293005.0000 14585960.0000 10061810.0000 16986640.0000 13736870.0000 10035840.0000 8033504.0000 13219740.0000 8782501.0000 6451864.0000 6156251.0000 8964333.0000 10636600.0000 5762375.0000 5991380.0000 8737127.0000 8844654.0000 CL 76:11 8954858.0000 7561479.0000 15993590.0000 8252519.0000 5997606.0000 5056546.0000 8918012.0000 3386544.0000 7757980.0000 11138450.0000 10284400.0000 7224010.0000 12008670.0000 7721615.0000 6510208.0000 6894630.0000 29840400.0000 20692970.0000 34995680.0000 27244630.0000 21482110.0000 17608960.0000 20508440.0000 13186070.0000 11741790.0000 13780860.0000 17352070.0000 22909480.0000 12610670.0000 15526820.0000 19317420.0000 16519200.0000 CL 76:12 19086190.0000 14592120.0000 28606050.0000 17180210.0000 13863270.0000 10538230.0000 19260610.0000 5164437.0000 14935170.0000 22572870.0000 21765080.0000 16789680.0000 25550630.0000 15245010.0000 13898590.0000 14404110.0000 68335940.0000 46322050.0000 54399130.0000 58019370.0000 50070420.0000 40121200.0000 57421720.0000 34866920.0000 24262460.0000 33165670.0000 45205930.0000 29043160.0000 31858500.0000 32641370.0000 49620980.0000 39944830.0000 CL 78:13 2546234.0000 2447926.0000 4502755.0000 2705375.0000 2036964.0000 1406288.0000 2504406.0000 633611.6000 1799727.0000 2037355.0000 1932517.0000 1061548.0000 3390908.0000 972859.1000 1064595.0000 1062557.0000 8241298.0000 5587208.0000 7590325.0000 6753710.0000 6535251.0000 4952406.0000 6092288.0000 5153679.0000 2758807.0000 2336509.0000 3199038.0000 4406047.0000 1965676.0000 1912222.0000 4282382.0000 4108983.0000 PA 34:3 109522.4000 617630.1000 73448.2900 15977.5100 35115.9300 12413.0100 49466.3600 19344.4800 92616.5300 131273.3000 17871.8600 12065.9400 12714.2000 48017.7300 18901.2700 43757.7400 90453.6200 81091.1200 696917.9000 27284.1400 16914.4600 23374.6000 436175.5000 PG 33:1 178222.0000 166549.5000 189852.8000 191052.8000 153049.7000 208141.2000 297715.3000 135254.0000 151322.6000 105606.7000 99622.9100 112032.6000 100475.7000 150964.2000 144033.6000 99131.3800 294000.4000 140688.1000 164277.0000 219350.4000 211545.8000 276723.4000 214622.8000 106776.0000 200000.2000 49764.5100 195946.9000 89980.3900 126348.8000 115446.0000 75094.5700 90511.9500 PG 34:0 19408000.0000 18582850.0000 19119380.0000 22457590.0000 25651840.0000 29014820.0000 29117650.0000 23702140.0000 20352430.0000 29332270.0000 26695220.0000 33260000.0000 28235340.0000 39904060.0000 28315000.0000 27978520.0000 18832820.0000 16339190.0000 17372870.0000 18265440.0000 25087590.0000 31074590.0000 26307860.0000 22288100.0000 27268870.0000 30301390.0000 41605900.0000 15289700.0000 39985510.0000 33144310.0000 36976800.0000 42887010.0000 PG 34:1 75133830.0000 63157300.0000 87747800.0000 74837560.0000 62938800.0000 77560920.0000 73758190.0000 49179570.0000 52834220.0000 77643650.0000 68781970.0000 74930020.0000 65939700.0000 87320270.0000 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26887.2600 37634.1300 19010.4700 32991.5800 31235.0300 33822.3900 26182.6700 PG 36:1 34224220.0000 31626340.0000 44870770.0000 21276180.0000 15868900.0000 34363040.0000 34473540.0000 18810900.0000 44991140.0000 52025120.0000 55320490.0000 59561340.0000 58789570.0000 29695000.0000 42505000.0000 30339350.0000 116041700.0000 80128870.0000 99017180.0000 89891390.0000 86170340.0000 75875300.0000 107108900.0000 56286300.0000 73096500.0000 78143710.0000 60958900.0000 113205200.0000 107998800.0000 94710620.0000 92377040.0000 63460670.0000 PG 36:2 25154290.0000 12872260.0000 10519170.0000 8300091.0000 11794300.0000 15097550.0000 12582060.0000 15045000.0000 2715659.0000 8008867.0000 4640645.0000 7986600.0000 4341640.0000 10505390.0000 7890587.0000 6277726.0000 2084169.0000 1026431.0000 1943743.0000 2428520.0000 1316136.0000 2060820.0000 1932164.0000 1856180.0000 6147342.0000 1875091.0000 2875378.0000 1191533.0000 2357966.0000 2903475.0000 2511856.0000 2047320.0000 PG 36:3 13930660.0000 8368834.0000 7594743.0000 5994046.0000 10068170.0000 10311990.0000 9934257.0000 14341700.0000 2722062.0000 7596366.0000 4728182.0000 8826290.0000 4258338.0000 9557005.0000 8267194.0000 5481727.0000 1378008.0000 639947.4000 869510.7000 1413083.0000 922558.9000 1256338.0000 1395133.0000 1225288.0000 5336471.0000 1570755.0000 1972145.0000 965759.1000 1987841.0000 2399254.0000 1842566.0000 1507831.0000 PG 36:4 574099.4000 1028126.0000 406805.0000 1831311.0000 666382.1000 1726166.0000 879113.8000 1448193.0000 251292.0000 1345530.0000 1828028.0000 489228.6000 455311.8000 1473843.0000 1232651.0000 948559.1000 2231270.0000 1333400.0000 315975.7000 1306059.0000 795642.9000 749546.6000 739005.4000 862021.6000 823504.9000 279327.3000 1267775.0000 844845.2000 2012186.0000 1428628.0000 798617.4000 453823.3000 PG 36:5 221428.7000 166247.6000 152631.0000 149484.1000 361081.4000 297912.0000 245882.8000 436566.3000 65683.3100 215627.2000 146224.9000 290837.6000 100457.0000 281684.0000 213467.5000 146670.0000 25235.8500 9861.2790 18136.5600 24207.2100 25966.1400 27923.6100 32707.5600 24110.7800 148103.9000 41874.4200 61033.7300 19864.3100 57661.0300 67877.2800 43745.1300 35646.5000 PG 38:2 675936.9000 383831.8000 341291.0000 356212.3000 558971.2000 777088.4000 995422.4000 541344.5000 139561.4000 363376.6000 305656.8000 368299.1000 283892.6000 475510.0000 358407.9000 321482.2000 109053.7000 112077.5000 118918.0000 122849.3000 203943.6000 199107.3000 150600.2000 295426.6000 144718.8000 138815.7000 84870.1600 156572.1000 135369.8000 175479.2000 140767.0000 PG 38:5 805521.3000 478627.2000 622951.3000 519076.9000 568322.7000 464278.2000 484276.1000 475573.2000 263340.3000 489514.7000 509080.4000 811465.0000 579262.6000 458623.6000 435747.2000 332015.7000 478863.9000 327623.1000 344070.9000 357217.6000 283315.2000 292344.7000 387295.9000 232249.7000 530593.4000 443098.7000 707761.6000 231718.0000 416781.4000 373819.7000 339021.4000 273462.8000 PG 40:6 1208964.0000 914124.7000 807494.9000 761635.1000 903633.4000 1035466.0000 1130811.0000 847264.8000 164362.8000 469706.1000 384199.4000 847992.5000 434077.5000 512013.7000 631235.2000 557243.9000 621551.9000 362203.2000 703642.9000 464016.8000 609439.5000 502155.1000 741566.5000 367920.6000 428089.4000 286466.6000 352805.2000 136992.8000 288552.0000 292898.4000 270765.8000 184350.0000 PG 40:7 1643436.0000 1028528.0000 859760.8000 841713.5000 568130.3000 686392.3000 902528.9000 659807.7000 428127.0000 618288.8000 645060.1000 865654.8000 757720.1000 677428.4000 473738.3000 380646.3000 1027479.0000 644728.4000 911919.9000 555190.2000 435110.6000 405688.4000 627643.3000 348696.0000 750239.5000 261061.3000 507764.5000 415933.1000 340890.4000 249639.7000 335026.2000 285527.4000 PG 40:8 826217.4000 652483.3000 561671.3000 650433.6000 518896.6000 513702.8000 633260.3000 582534.2000 417188.6000 635584.9000 635944.7000 834876.4000 675490.0000 732648.2000 332847.6000 236476.0000 642852.4000 402949.3000 530425.7000 294868.3000 354212.7000 280700.7000 427736.5000 244246.7000 688962.4000 223274.0000 456480.3000 402988.9000 360077.8000 244709.7000 325116.3000 219231.2000 PG 42:10 363179.0000 218392.5000 278778.6000 224549.5000 200462.1000 171920.4000 189369.7000 188588.1000 146659.8000 218986.4000 180528.7000 285011.7000 289892.6000 213555.5000 147403.1000 135329.0000 306687.7000 234769.9000 324556.0000 124242.0000 151444.3000 91703.5100 163403.5000 90380.9900 224465.5000 98736.9400 211537.3000 223612.0000 115162.4000 80769.7400 91947.2900 91545.6100 PG 42:9 184668.4000 136375.3000 221921.2000 146571.8000 247154.0000 188738.8000 194239.7000 191661.3000 49453.9400 103092.1000 86951.1400 268416.8000 139380.5000 108723.4000 163110.1000 179463.4000 111999.8000 75180.7700 158135.9000 67706.7400 129734.8000 79932.2000 128980.0000 63015.3400 111218.5000 101281.1000 100893.1000 53305.5000 71900.1100 69437.5400 45966.4000 47093.8800 PG 44:11 282704.8000 269454.6000 253245.2000 200726.6000 181857.2000 232063.6000 245796.0000 181284.9000 110647.5000 139172.4000 112351.1000 255282.7000 193144.9000 150420.6000 145516.3000 163406.2000 337912.9000 217671.3000 717477.1000 128548.4000 228021.4000 161062.8000 260739.0000 136027.1000 145259.8000 81210.5800 129149.8000 184135.4000 112473.5000 89406.9600 90036.1300 73989.8000 PG 44:12 400931.3000 263523.6000 228895.0000 186126.2000 109137.1000 126005.9000 132081.7000 109828.0000 213977.7000 171344.0000 158862.6000 191726.3000 292650.8000 145679.2000 81130.4900 86423.6800 543640.6000 369278.3000 1172088.0000 190000.5000 181479.4000 120174.6000 215900.3000 145641.4000 209248.6000 65399.9000 171387.3000 729690.6000 138064.0000 79793.5000 107785.0000 112165.2000 MS_METABOLITE_DATA_END #METABOLITES METABOLITES_START metabolite_name pubchem_id inchi_key kegg_id other_id other_id_type ri ri_type moverz_quant CL 71:4 CL 72:5 CL 72:6 CL 72:7 CL 72:8 CL 72:9 CL 73:8 CL 74:10 CL 74:11 CL 74:6 CL 74:7 CL 74:8 CL 74:9 CL 76:10 CL 76:11 CL 76:12 CL 78:13 PA 34:3 PG 33:1 PG 34:0 PG 34:1 PG 34:2 PG 34:3 PG 36:1 PG 36:2 PG 36:3 PG 36:4 PG 36:5 PG 38:2 PG 38:5 PG 40:6 PG 40:7 PG 40:8 PG 42:10 PG 42:9 PG 44:11 PG 44:12 METABOLITES_END #END