{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000063","ANALYSIS_ID":"AN000101","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Biomarkers for Depression in CSF and Plasma in a Population Sample","PROJECT_TYPE":"biomarker study","PROJECT_SUMMARY":"This West Coast Metabolomics Center pilot and feasibility project was granted Roel Ophoff (UC Los Angeles) and Steve Horwath (UC Los Angeles). Major disorder (MDD) is one of the most debilitating disorders in the United States a 12-month prevalence of 6.7% in the adult population. The disorder affects of Americans daily and is a major health concern with enormous economic cost society at large. Criteria for MDD diagnosis and treatment are based on various and symptoms not always fitting into strict diagnostic categories such as Despite various known risk factors (such as family history, age, and gender), markers supporting diagnosis or prediction of MDD are unavailable. We have cerebrospinal fluid (CSF) and peripheral blood of more than 600 subjects from general population. For each of the participants we also obtained biometric as well as behavioral trait measures. One of the measures is the Beck Inventory (BDI), a well-established questionnaire for measuring severity of Based on the BDI, roughly 5% of participants suffer from severe depressive while most of these subjects are not under treatment or receiving any for depression In the current investigation, untargeted analysis of primary was conducted on age and gender matched human cerebrospinal fluid (CSF) and from subjects suffering with MDD ( n=50) and control subjects (n=50). Subjects diagnosed as having MDD based on the Beck Depression Inventory. The primary of this study were to 1) identify metabolites which discriminate between with and without depression symptoms in the CSF and plasma and 2) how changes between CSF and plasma.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility,451 Health Sciences Drive, Davis, 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","FUNDING_SOURCE":"NIH U24DK097154"},

"STUDY":{"STUDY_TITLE":"Biomarkers for Depression in Human Cerebrospinal Fluid in a Population Sample","STUDY_TYPE":"biomarker study","STUDY_SUMMARY":"This West Coast Metabolomics Center pilot and feasibility project was granted Roel Ophoff (UC Los Angeles ) and Steve Horwath (UC Los Angeles ) . Major disorder (MDD) is one of the most debilitating disorders in the United States a 12-month prevalence of 6.7% in the adult population. The disorder affects of Americans daily and is a major health concern with enormous economic cost society at large. Criteria for MDD diagnosis and treatment are based on various and symptoms not always fitting into strict diagnostic categories such as Despite various known risk factors (such as family history, age, and gender), markers supporting diagnosis or prediction of MDD are unavailable. We have cerebrospinal fluid (CSF) and peripheral blood of more than 600 subjects from general population. For each of the participants we also obtained biometric as well as behavioral trait measures. One of the measures is the Beck Inventory (BDI), a well-established questionnaire for measuring severity of Based on the BDI, roughly 5% of participants suffer from severe depressive while most of these subjects are not under treatment or receiving any for depression In the current investigation, untargeted analysis of primary was conducted on age and gender matched human cerebrospinal fluid (CSF) and from subjects suffering with MDD ( n=50) and control subjects (n=50). were diagnosed as having MDD based on the Beck Depression Inventory.The primary of this study were to 1) identify metabolites which discriminate between with and without depression symptoms in the CSF and plasma and 2) how changes between CSF and plasma. ","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility451 Health Sciences DriveDavis, CA","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","NUM_GROUPS":"3","TOTAL_SUBJECTS":"105","STUDY_COMMENTS":"2014-03-03 13:37:37.766"},

"SUBJECT":{"SUBJECT_TYPE":"CSF","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"CSF_48",
"Sample ID":"140317ajlsa01_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_17",
"Sample ID":"140317ajlsa04_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_40",
"Sample ID":"140317ajlsa08_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_22",
"Sample ID":"140317ajlsa09_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_37",
"Sample ID":"140317ajlsa10_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_02",
"Sample ID":"140317ajlsa11_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_46",
"Sample ID":"140317ajlsa13_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_27",
"Sample ID":"140317ajlsa18_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_03",
"Sample ID":"140317ajlsa20_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_16",
"Sample ID":"140317ajlsa23_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_14",
"Sample ID":"140317ajlsa24_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_01",
"Sample ID":"140317ajlsa28_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_35",
"Sample ID":"140317ajlsa31_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_08",
"Sample ID":"140317ajlsa32_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_39",
"Sample ID":"140317ajlsa33_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_20",
"Sample ID":"140317ajlsa38_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_45",
"Sample ID":"140317ajlsa41_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_07",
"Sample ID":"140317ajlsa42_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_15",
"Sample ID":"140317ajlsa49_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_28",
"Sample ID":"140317ajlsa50_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_38",
"Sample ID":"140318ajlsa01_2",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_42",
"Sample ID":"140318ajlsa03_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_18",
"Sample ID":"140318ajlsa06_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_32",
"Sample ID":"140318ajlsa08_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_31",
"Sample ID":"140318ajlsa13_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_50",
"Sample ID":"140318ajlsa14_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_34",
"Sample ID":"140318ajlsa16_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_23",
"Sample ID":"140318ajlsa20_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_25",
"Sample ID":"140318ajlsa21_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_44",
"Sample ID":"140318ajlsa24_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_09",
"Sample ID":"140318ajlsa27_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_43",
"Sample ID":"140318ajlsa28_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_49",
"Sample ID":"140318ajlsa30_1",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_12",
"Sample ID":"140318ajlsa31_4",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_04",
"Sample ID":"140318ajlsa32_4",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_36",
"Sample ID":"140318ajlsa33_4",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_24",
"Sample ID":"140318ajlsa34_4",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_41",
"Sample ID":"140318ajlsa36_4",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_30",
"Sample ID":"140318ajlsa37_4",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_29",
"Sample ID":"140318ajlsa41_3",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_05",
"Sample ID":"140318ajlsa44_3",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_47",
"Sample ID":"140318ajlsa47_3",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_21",
"Sample ID":"140318ajlsa48_3",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_10",
"Sample ID":"140318ajlsa49_3",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_11",
"Sample ID":"140319ajlsa01_2",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_06",
"Sample ID":"140319ajlsa03_2",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_33",
"Sample ID":"140319ajlsa04_2",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_19",
"Sample ID":"140319ajlsa05_2",
"Factors":{"Source":"Group 1 - Score 0"}
},
{
"Subject ID":"CSF_69",
"Sample ID":"140317ajlsa02_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_52",
"Sample ID":"140317ajlsa03_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_98",
"Sample ID":"140317ajlsa05_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_61",
"Sample ID":"140317ajlsa06_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_59",
"Sample ID":"140317ajlsa07_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_89",
"Sample ID":"140317ajlsa12_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_85",
"Sample ID":"140317ajlsa14_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_81",
"Sample ID":"140317ajlsa16_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_58",
"Sample ID":"140317ajlsa17_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_96",
"Sample ID":"140317ajlsa19_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_51",
"Sample ID":"140317ajlsa21_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_62",
"Sample ID":"140317ajlsa22_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_80",
"Sample ID":"140317ajlsa25_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_66",
"Sample ID":"140317ajlsa26_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_91",
"Sample ID":"140317ajlsa27_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_77",
"Sample ID":"140317ajlsa29_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_92",
"Sample ID":"140317ajlsa30_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_78",
"Sample ID":"140317ajlsa34_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_71",
"Sample ID":"140317ajlsa35_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_76",
"Sample ID":"140317ajlsa36_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_84",
"Sample ID":"140317ajlsa37_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_55",
"Sample ID":"140317ajlsa39_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_65",
"Sample ID":"140317ajlsa43_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_95",
"Sample ID":"140317ajlsa45_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_73",
"Sample ID":"140317ajlsa46_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_75",
"Sample ID":"140317ajlsa47_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_56",
"Sample ID":"140317ajlsa48_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_60",
"Sample ID":"140318ajlsa02_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_67",
"Sample ID":"140318ajlsa04_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_94",
"Sample ID":"140318ajlsa07_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_54",
"Sample ID":"140318ajlsa09_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_53",
"Sample ID":"140318ajlsa10_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_68",
"Sample ID":"140318ajlsa11_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_99",
"Sample ID":"140318ajlsa12_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_64",
"Sample ID":"140318ajlsa15_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_72",
"Sample ID":"140318ajlsa17_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_87",
"Sample ID":"140318ajlsa22_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_79",
"Sample ID":"140318ajlsa25_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_90",
"Sample ID":"140318ajlsa26_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_63",
"Sample ID":"140318ajlsa29_1",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_88",
"Sample ID":"140318ajlsa35_4",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_74",
"Sample ID":"140318ajlsa39_4",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_70",
"Sample ID":"140318ajlsa40_4",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_82",
"Sample ID":"140318ajlsa42_3",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_83",
"Sample ID":"140318ajlsa43_3",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_86",
"Sample ID":"140318ajlsa45_3",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_100",
"Sample ID":"140318ajlsa46_3",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_97",
"Sample ID":"140318ajlsa50_3",
"Factors":{"Source":"Group 2 - Score 50"}
},
{
"Subject ID":"CSF_57",
"Sample ID":"140319ajlsa02_2",
"Factors":{"Source":"Group 2 - Score 50"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"CSF_Extraction_Protocol_Primary_Metabolites.docx","SAMPLE_SPIKING":"FAMEs"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 6890N","COLUMN_NAME":"Restek Corporation Rtx-5Sil MS","METHODS_FILENAME":"Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf","COLUMN_PRESSURE":"7.7 PSI (initial condition)","COLUMN_TEMPERATURE":"50 - 330C","FLOW_RATE":"1 ml/min","INJECTION_TEMPERATURE":"50C ramped to 250C by 12C/s","SAMPLE_INJECTION":"0.5l","OVEN_TEMPERATURE":"50C for 1 min, then ramped at 20C/min to 330C, held constant for 5 min","TRANSFERLINE_TEMPERATURE":"230C","WASHING_BUFFER":"Ethyl Acetate","SAMPLE_LOOP_SIZE":"30 m length x 0.25 mm internal diameter","RANDOMIZATION_ORDER":"Excel generated"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"WCMC Metabolomics Core","ACQUISITION_PARAMETERS_FILE":"Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf","SOFTWARE_VERSION":"ChromaTOF","OPERATOR_NAME":"Joyce Lopez","PROCESSING_PARAMETERS_FILE":"Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf","DETECTOR_TYPE":"TOF","DATA_FORMAT":".peg, .txt, .cdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Leco GC-TOF","INSTRUMENT_TYPE":"GC-TOF","MS_TYPE":"EI","ION_MODE":"POSITIVE","ION_SOURCE_TEMPERATURE":"250°C","IONIZATION_ENERGY":"70eV","MASS_ACCURACY":"Nominal","SOURCE_TEMPERATURE":"250°C","SCANNING_RANGE":"80-500 Da"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

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}