{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000075","ANALYSIS_ID":"AN000120","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Combined Metabolomics and Lipidomics of Type 1 Diabetes","PROJECT_TYPE":"MS study","PROJECT_SUMMARY":"This West Coast Metabolomics Center pilot and feasibility project granted to Bell  (University of Chicago) and collaborator Manami Hara uses a multi-omics to investigate the metabolome (primary metabolites), lipidome (complex lipids) signaling lipids (oxylipins) in the plasma of Non-obese Diabetic (NOD) mice progressed to Type 1 Diabetes Mellitus (T1D) and those that did not. NOD Mice were assessed as diabetic or non-diabetic based on their fasting (4hr) blood levels at sacrifice, which defined 30 hyperglycemic (glucose = 250 mg/dL) and normoglycemic animals. The primary objective of this study was to identify biomarkers associated with beta-cell destruction/survival and T1D progression.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility,451 Health Sciences Drive, Davis, 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","FUNDING_SOURCE":"NIH U24DK097154"},

"STUDY":{"STUDY_TITLE":"Combined Metabolomics and Lipidomics of Type 1 Diabetes (QTOF)","STUDY_TYPE":"biomarker study","STUDY_SUMMARY":"This West Coast Metabolomics Center pilot and feasibility project granted to Bell  (University of Chicago) and collaborator Manami Hara uses a multi-omics to investigate the metabolome (primary metabolites), lipidome (complex lipids) signaling lipids (oxylipins) in the plasma of Non-obese Diabetic (NOD) mice progressed to Type 1 Diabetes Mellitus (T1D) and those that did not. NOD Mice were assessed as diabetic or non-diabetic based on their fasting (4hr) blood levels at sacrifice, which defined 30 hyperglycemic (glucose = 250 mg/dL) and normoglycemic animals. The primary objective of this study was to identify biomarkers associated with beta-cell destruction/survival and T1D progression.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility451 Health Sciences DriveDavis, CA","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","NUM_GROUPS":"2","TOTAL_SUBJECTS":"71","STUDY_COMMENTS":"Lipidomics profiles for study"},

"SUBJECT":{"SUBJECT_TYPE":"Plasma","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Inj003_Manami_7_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj004_Manami_52_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj005_Manami_40_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj006_Manami_76_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj010_Manami_63_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj011_Manami_55_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj012_Manami_73_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj017_Manami_13_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj019_Manami_53_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj020_Manami_62_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj030_Manami_47_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj033_Manami_17_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj035_Manami_54_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj036_Manami_19_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj039_Manami_15_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj043_Manami_49_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj048_Manami_10_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj050_Manami_20_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj052_Manami_56_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj054_Manami_4_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj055_Manami_12_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj056_Manami_67_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj058_Manami_32_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj061_Manami_36_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj062_Manami_25_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj063_Manami_6_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj064_Manami_30_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj068_Manami_37_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj069_Manami_60_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj071_Manami_57_CSHMS_6530B.d",
"Factors":{"Diagnosis":"diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj001_Manami_2_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj002_Manami_27_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj007_Manami_51_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj008_Manami_59_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj009_Manami_39_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj013_Manami_29_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj014_Manami_22_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj015_Manami_5_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj016_Manami_31_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj018_Manami_50_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj021_Manami_21_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj022_Manami_14_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj023_Manami_41_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj024_Manami_43_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj025_Manami_33_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj026_Manami_23_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj027_Manami_18_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj028_Manami_35_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj029_Manami_34_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj031_Manami_9_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj032_Manami_64_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj034_Manami_66_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj037_Manami_46_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj038_Manami_8_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj040_Manami_26_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj041_Manami_69_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj042_Manami_61_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj044_Manami_3_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj045_Manami_58_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj046_Manami_24_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj047_Manami_16_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj049_Manami_48_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj051_Manami_44_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj053_Manami_11_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj057_Manami_1_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj059_Manami_28_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj060_Manami_42_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj065_Manami_38_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj066_Manami_45_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj067_Manami_68_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Inj070_Manami_65_CSHMS_6530B.d",
"Factors":{"Diagnosis":"non-diabetic"}
},
{
"Subject ID":"-",
"Sample ID":"Biorec1_CSHMS_6530B.d",
"Factors":{"Diagnosis":"QC - quality check sample"}
},
{
"Subject ID":"-",
"Sample ID":"Biorec3_CSHMS_6530B.d",
"Factors":{"Diagnosis":"QC - quality check sample"}
},
{
"Subject ID":"-",
"Sample ID":"Biorec4_CSHMS_6530B.d",
"Factors":{"Diagnosis":"QC - quality check sample"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","COLLECTION_PROTOCOL_FILENAME":"Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics.pdf"},

"TREATMENT":{"TREATMENT_SUMMARY":"-","TREATMENT_PROTOCOL_FILENAME":"Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics.pdf"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"UPLC","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity CSH C18 (100 x 2.1mm, 1.7um) Pre-Column","METHODS_FILENAME":"Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics.pdf","COLUMN_PRESSURE":"450-850 bar","COLUMN_TEMPERATURE":"65 C","FLOW_GRADIENT":"15% B to 99% B","FLOW_RATE":"0.6 mL/min","INTERNAL_STANDARD":"See data dictionary","RETENTION_TIME":"See data dictionary","SAMPLE_INJECTION":"1.67 uL","SOLVENT_A":"60:40 Acetonitrile:Water +10mM Ammonium Formate +10mM Formic Acid","SOLVENT_B":"9:1 Isopropanol:Acetonitrile +10mM Ammonium Formate +10mM Formic Acid","ANALYTICAL_TIME":"13 min","CAPILLARY_VOLTAGE":"3500","TIME_PROGRAM":"15 min","WEAK_WASH_SOLVENT_NAME":"Isopropanol","WEAK_WASH_VOLUME":"5 seconds","STRONG_WASH_SOLVENT_NAME":"Same","TARGET_SAMPLE_TEMPERATURE":"Autosampler temp 4 C","RANDOMIZATION_ORDER":"Excel"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","SOFTWARE_VERSION":"MassHunter","DETECTOR_TYPE":"TOF MCP","DATA_FORMAT":".d"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6550 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","CAPILLARY_VOLTAGE":"3500","COLLISION_GAS":"Nitrogen","DRY_GAS_FLOW":"13 l/min","DRY_GAS_TEMP":"200","FRAGMENT_VOLTAGE":"175","FRAGMENTATION_METHOD":"Auto MS/MS","ION_SOURCE_TEMPERATURE":"325","ION_SPRAY_VOLTAGE":"1000","IONIZATION":"Neg","PRECURSOR_TYPE":"Intact Molecule","REAGENT_GAS":"Nitrogen","SOURCE_TEMPERATURE":"325 C","DATAFORMAT":".d","DESOLVATION_GAS_FLOW":"11 l/min","DESOLVATION_TEMPERATURE":"350 C","NEBULIZER":"35 psig","OCTPOLE_VOLTAGE":"750","SCAN_RANGE_MOVERZ":"60-1700 Da","SCANNING_CYCLE":"2 Hz","SCANNING_RANGE":"60-1700 Da","SKIMMER_VOLTAGE":"65"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

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