{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000076","ANALYSIS_ID":"AN000122","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"CESB Lung Cancer Project","PROJECT_TYPE":"MS/NMR","PROJECT_SUMMARY":"Influence of Microenvironmental Nutrient Availability on Systems Biochemistry Lung Cancer Cells and Immuomodulation. This cell-based project aims to gain a understanding of lung cancer metabolism and dysregulations as a function of key factors that govern lung cancer cell development and its ability to evade surveillance. The factors to be examined include the availability of key crucial to cancer proliferation, survival, metastasis, and immunoregulation.","INSTITUTE":"University of Kentucky","DEPARTMENT":"Toxicology","LAST_NAME":"Fan","FIRST_NAME":"Teresa","ADDRESS":"-","EMAIL":"twmfan@gmail.com","PHONE":"-"},

"STUDY":{"STUDY_TITLE":"A549 Cell Study","STUDY_TYPE":"tracer","STUDY_SUMMARY":"13C-Glu and 13C/15N-Gln SIRM study of A549 cells","INSTITUTE":"University of Kentucky","DEPARTMENT":"School of Medicine","LAST_NAME":"Yan","FIRST_NAME":"Jun","ADDRESS":"-","EMAIL":"jun.yan@louisville.edu","PHONE":"-","NUM_GROUPS":"3","TOTAL_SUBJECTS":"6"},

"SUBJECT":{"SUBJECT_TYPE":"cell culture","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENOTYPE_STRAIN":"A549","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"13C/15N-Gln-plate-1",
"Sample ID":"13C/15N-Gln-plate-1-cells",
"Factors":{"Labeling":"13C/15N-Gln"},
"Additional sample data":{"dry_weight(g)":"0.0012","polar extract wet_weight(g)":"0.3749","cells wet_weight(g)":"1.5829","protocol.BCA_protein(mg)":"0.7276"}
},
{
"Subject ID":"13C/15N-Gln-plate-2",
"Sample ID":"13C/15N-Gln-plate-2-cells",
"Factors":{"Labeling":"13C/15N-Gln"},
"Additional sample data":{"dry_weight(g)":"0.0010","polar extract wet_weight(g)":"0.4441","cells wet_weight(g)":"1.7275","protocol.BCA_protein(mg)":"0.7277"}
},
{
"Subject ID":"13C-Glc-plate-1",
"Sample ID":"13C-Glc-plate-1-cells",
"Factors":{"Labeling":"13C-Glc"},
"Additional sample data":{"dry_weight(g)":"0.0016","polar extract wet_weight(g)":"0.3775","cells wet_weight(g)":"1.7160","protocol.BCA_protein(mg)":"0.6682"}
},
{
"Subject ID":"13C-Glc-plate-2",
"Sample ID":"13C-Glc-plate-2-cells",
"Factors":{"Labeling":"13C-Glc"},
"Additional sample data":{"dry_weight(g)":"0.0011","polar extract wet_weight(g)":"0.3828","cells wet_weight(g)":"1.7425","protocol.BCA_protein(mg)":"0.7482"}
},
{
"Subject ID":"unlabeled-plate-1",
"Sample ID":"unlabeled-plate-1-cells",
"Factors":{"Labeling":"unlabeled"},
"Additional sample data":{"dry_weight(g)":"0.0015","polar extract wet_weight(g)":"0.3794","cells wet_weight(g)":"1.7205","protocol.BCA_protein(mg)":"0.7713"}
},
{
"Subject ID":"unlabeled-plate-2",
"Sample ID":"unlabeled-plate-2-cells",
"Factors":{"Labeling":"unlabeled"},
"Additional sample data":{"dry_weight(g)":"0.0012","polar extract wet_weight(g)":"0.4242","cells wet_weight(g)":"1.7368","protocol.BCA_protein(mg)":"0.6368"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"cell_quench_collection","COLLECTION_PROTOCOL_FILENAME":"14Jun17_Fan_Quench_cell_tissue_SOP.pdf","COLLECTION_PROTOCOL_COMMENTS":"Collection and quench of cells in tissue culture"},

"TREATMENT":{"TREATMENT_SUMMARY":"13C-Glc|13C/15N-Gln|unlabeled","TREATMENT_PROTOCOL_FILENAME":"14Jun17Fan_Cell_Tracer_Ex_SOP.pdf","TREATMENT_PROTOCOL_COMMENTS":"Cell culture in 13C-Glc-labeled media | 13C/15N-Gln-labeled media | unlabeled for 24h"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"protein_extraction|polar_preparation|polar_extraction","SAMPLEPREP_PROTOCOL_FILENAME":"14June20Fan_Prot_Quant_SOP.pdf|14Jun30Fan_Prep_Polar_SOP.pdf|14Jun30Fan_Extract_Polar_Lipid_Prot_SOP.pdf","SAMPLEPREP_PROTOCOL_COMMENTS":"Protein extraction and quantification|Preparation of polar samples for GCMS and extraction from cells."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"RCSIRM"},

"MS":{"INSTRUMENT_NAME":"Thermo ITQ","INSTRUMENT_TYPE":"GC-ITQ","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"GCMS Analytical Experiment with detection of compounds by comparison to Derivatization is done with MTBSTFA GCMS"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

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