{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000085","ANALYSIS_ID":"AN000137","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Systems Biology for EnteroPathogens","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"sysbep.org","INSTITUTE":"Pacific Northwest National Laboratory","DEPARTMENT":"Biological Separation and Mass Spectrometry","LAST_NAME":"Joshua","FIRST_NAME":"Adkins","ADDRESS":"-","EMAIL":"Joshua.Adkins@pnnl.gov","PHONE":"-"},

"STUDY":{"STUDY_TITLE":"Salmonella Modulates Metabolism during Growth under Conditions that Induce of Virulence Genes","STUDY_TYPE":"growth conditions, timecourse","STUDY_SUMMARY":"Salmonella enterica serovar Typhimurium (S. Typhimurium) is a facultative that uses complex mechanisms to invade and proliferate within mammalian host To investigate possible contributions of metabolic processes to virulence in S. grown under conditions known to induce expression of virulence genes, we used a systems biology approach coupled with genome scale modeling. First, we distinct metabolite profiles associated with bacteria grown in either rich or media and report the most comprehensive coverage of the S. Typhimurium to date. Second, we applied an omics-informed genome scale modeling analysis of functional consequences of adaptive alterations in S. Typhimurium metabolism growth under our conditions. Modeling efforts highlighted a decreased cellular to both produce and utilize intracellular amino acids during stationary phase in virulence conditions, despite significant abundance increases for these as observed by our metabolomics measurements. Furthermore, analyses of omics in the context of the metabolic model indicated rewiring of the metabolic to support pathways associated with virulence. For example, cellular of polyamines were perturbed, as well as the predicted capacity for secretion uptake.","INSTITUTE":"Pacific Northwest National Laboratory","DEPARTMENT":"Biological Separation and Mass Spectrometry","LAST_NAME":"Metz","FIRST_NAME":"Thomas","ADDRESS":"-","EMAIL":"thomas.metz@pnnl.gov","PHONE":"-","NUM_GROUPS":"3","TOTAL_SUBJECTS":"18"},

"SUBJECT":{"SUBJECT_TYPE":"Cells","SUBJECT_SPECIES":"Salmonella typhimurium","TAXONOMY_ID":"90371","GENOTYPE_STRAIN":"CDC 6516-60","CELL_BIOSOURCE_OR_SUPPLIER":"ATCC 14028","SPECIES_GROUP":"Microorganism"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LB_1",
"Factors":{"Growth Conditions":"Luria-Bertani (LB) medium","Time Harvested":">2.5hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LB_2",
"Factors":{"Growth Conditions":"Luria-Bertani (LB) medium","Time Harvested":">2.5hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LB_3",
"Factors":{"Growth Conditions":"Luria-Bertani (LB) medium","Time Harvested":">2.5hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LPM4_1",
"Factors":{"Growth Conditions":"Low pH, low magnesium, low iron (LPM) medium","Time Harvested":"4hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LPM4_2",
"Factors":{"Growth Conditions":"Low pH, low magnesium, low iron (LPM) medium","Time Harvested":"4hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LPM4_3",
"Factors":{"Growth Conditions":"Low pH, low magnesium, low iron (LPM) medium","Time Harvested":"4hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LPM20_1",
"Factors":{"Growth Conditions":"Low pH, low magnesium, low iron (LPM) medium","Time Harvested":"20hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LPM20_2",
"Factors":{"Growth Conditions":"Low pH, low magnesium, low iron (LPM) medium","Time Harvested":"20hour"}
},
{
"Subject ID":"-",
"Sample ID":"SBEP_STM_LPM20_3",
"Factors":{"Growth Conditions":"Low pH, low magnesium, low iron (LPM) medium","Time Harvested":"20hour"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were harvested via centrifugation (4,000×g) and washed twice with PBS (Mediatech)","SAMPLE_TYPE":"Cell","COLLECTION_METHOD":"Centrifugation (4,000 x g)","COLLECTION_FREQUENCY":"Once","COLLECTION_TIME":"variable, see Treatments","VOLUMEORAMOUNT_COLLECTED":"All","STORAGE_CONDITIONS":"-80° C"},

"TREATMENT":{"TREATMENT_SUMMARY":"Luria-Bertani (LB) medium, hartvested after >2.5 hours | Low pH, low magnesium, iron (LPM) medium, harvested after 20 h | Low pH, low magnesium, low iron (LPM) harvested after 4 h | Low pH, low magnesium, low iron (LPM) medium, harvested 20 h | Low pH, low magnesium, low iron (LPM) medium, harvested after 4 h","TREATMENT_PROTOCOL_COMMENTS":"S. Typhimurium wild type cells (ATCC 14028) were used throughout this study and taken from a single colony on an agar plate, subsequently inoculated into 7 mL Luria-Bertani (LB) media (comprised of 1% tryptone, 0.5% yeast extract, and 1% chloride at pH 7), and incubated overnight at 37°C. The overnight culture was and the supernatant discarded. The cell pellets were suspended in LB media and again, and the supernatant was discarded. For LB cultures, the cell pellets subsequently suspended in 2 mL of LB media and used to inoculate 700 mL of LB a 2.8 L flask. After 160 min of growth, cells were harvested via centrifugation and washed twice with Dulbecco’s PBS (Mediatech) once cultures reached an of +1.0. / S. Typhimurium wild type cells (ATCC 14028) were used throughout study and were taken from a single colony on an agar plate, subsequently into 7 mL of Luria-Bertani (LB) media (comprised of 1% tryptone, 0.5% yeast and 1% sodium chloride at pH 7), and incubated overnight at 37°C. The culture was centrifuged and the supernatant discarded. The cell pellets were in LB media and centrifuged again, and the supernatant was discarded. To the Salmonella virulence program, cells were transferred to a low pH, low and low iron (LPM) medium comprised of 8 µM MgCL2, 0.5 µM ferric citrate, 5 KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol v/v, 0.00001% thiamine, 337 µM and 80 mM 2-(N-morpholino)ethanesulfonic acid at pH 5.8. Briefly, cell pellets the 7 mL LB media were suspended in LPM media and centrifuged. The supernatant discarded and cell pellets were suspended in 2 mL of LPM media and used to 700 mL of LPM in a 2.8 L flask. Cells were harvested after 20 h, then cells harvested via centrifugation (4,000×g) and washed twice with Dulbecco’s PBS Three biological replicates were performed for each of the conditions described S. Typhimurium wild type cells (ATCC 14028) were used throughout this study and taken from a single colony on an agar plate, subsequently inoculated into 7 mL Luria-Bertani (LB) media (comprised of 1% tryptone, 0.5% yeast extract, and 1% chloride at pH 7), and incubated overnight at 37°C. The overnight culture was and the supernatant discarded. The cell pellets were suspended in LB media and again, and the supernatant was discarded. To stimulate the Salmonella virulence cells were transferred to a low pH, low magnesium, and low iron (LPM) medium of 8 µM MgCL2, 0.5 µM ferric citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, glycerol v/v, 0.00001% thiamine, 337 µM H3PO4, and 80 mM acid at pH 5.8. Briefly, cell pellets from the 7 mL LB media were suspended in media and centrifuged. The supernatant was discarded and cell pellets were in 2 mL of LPM media and used to inoculate 700 mL of LPM in a 2.8 L flask. were harvested after 4 h, then cells were harvested via centrifugation and washed twice with Dulbecco’s PBS (Mediatech). Three biological replicates performed for each of the conditions described above. || S. Typhimurium wild cells (ATCC 14028) were used throughout this study and were taken from a single on an agar plate, subsequently inoculated into 7 mL of Luria-Bertani (LB) media of 1% tryptone, 0.5% yeast extract, and 1% sodium chloride at pH 7), and overnight at 37°C. The overnight culture was centrifuged and the supernatant The cell pellets were suspended in LB media and centrifuged again, and the was discarded. To stimulate the Salmonella virulence program, cells were to a low pH, low magnesium, and low iron (LPM) medium comprised of 8 µM MgCL2, µM ferric citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol v/v, thiamine, 337 µM H3PO4, and 80 mM 2-(N-morpholino)ethanesulfonic acid at pH Briefly, cell pellets from the 7 mL LB media were suspended in LPM media and The supernatant was discarded and cell pellets were suspended in 2 mL of LPM and used to inoculate 700 mL of LPM in a 2.8 L flask. Cells were harvested 20 h, then cells were harvested via centrifugation (4,000×g) and washed twice Dulbecco’s PBS (Mediatech). Three biological replicates were performed for of the conditions described above. || S. Typhimurium wild type cells (ATCC were used throughout this study and were taken from a single colony on an agar subsequently inoculated into 7 mL of Luria-Bertani (LB) media (comprised of 1% 0.5% yeast extract, and 1% sodium chloride at pH 7), and incubated overnight at The overnight culture was centrifuged and the supernatant discarded. The cell were suspended in LB media and centrifuged again, and the supernatant was To stimulate the Salmonella virulence program, cells were transferred to a low low magnesium, and low iron (LPM) medium comprised of 8 µM MgCL2, 0.5 µM citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol v/v, 0.00001% 337 µM H3PO4, and 80 mM 2-(N-morpholino)ethanesulfonic acid at pH 5.8. cell pellets from the 7 mL LB media were suspended in LPM media and The supernatant was discarded and cell pellets were suspended in 2 mL of LPM and used to inoculate 700 mL of LPM in a 2.8 L flask. Cells were harvested 4 h, then cells were harvested via centrifugation (4,000×g) and washed twice Dulbecco’s PBS (Mediatech). Three biological replicates were performed for of the conditions described above.","TREATMENT":"Abiotic","TREATMENT_ROUTE":"Media","CELL_GROWTH_CONTAINER":"700 mL of LB in a 2.8 L flask","CELL_MEDIA":"Luria-Bertani (LB) medium (1% tryptone, 0.5% yeast extract, and 1% sodium at pH 7) / low pH, low magnesium, and low iron (LPM) medium comprised of 8 µM 0.5 µM ferric citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol 0.00001% thiamine, 337 µM H3PO4, and 80 mM 2-(N-morpholino)ethanesulfonic acid pH 5.8/ low pH, low magnesium, and low iron (LPM) medium comprised of 8 µM 0.5 µM ferric citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol 0.00001% thiamine, 337 µM H3PO4, and 80 mM 2-(N-morpholino)ethanesulfonic acid pH 5.8 || low pH, low magnesium, and low iron (LPM) medium comprised of 8 µM 0.5 µM ferric citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol 0.00001% thiamine, 337 µM H3PO4, and 80 mM 2-(N-morpholino)ethanesulfonic acid pH 5.8 || low pH, low magnesium, and low iron (LPM) medium comprised of 8 µM 0.5 µM ferric citrate, 5 mM KCl, 7.5 mM NH4SO4, 0.5 mM K2SO4, 0.3% glycerol 0.00001% thiamine, 337 µM H3PO4, and 80 mM 2-(N-morpholino)ethanesulfonic acid pH 5.8","CELL_ENVIR_COND":"37° C","CELL_HARVESTING":"centrifugation (4,000×g) and washed twice with Dulbecco’s PBS (Mediatech)"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Cell pellets resuspended in ammonium bicarbonate, lysed via bead beating, with four volumes of chilled (-20°C) chloroform/methanol (2:1, v/v)","SAMPLEPREP_PROTOCOL_COMMENTS":"Cell pellets were stored at -80°C prior to thawing and were subsequently in an appropriate volume of 100 mM ammonium bicarbonate according to their wet to compensate for any differences in cell numbers. The cells were lysed by and the lysates were transferred into new tubes. Subsequently, 100 µL aliquots cell lysates were extracted with four volumes of chilled (-20°C) (2:1, v/v), and the aqueous phases after centrifugation were transferred to vials and dried in vacuo (SpeedVac; Thermo Scientific, Waltham, MA). All were kept at -80°C prior to chemical derivatization for GC-MS analysis. Dried extracts were briefly dried again to remove any residual water prior to derivatization. To protect carbonyl groups and reduce the number of tautomeric 20 µL of methoxyamine in pyridine (30 mg/mL) were added to each sample, by incubation at 37°C with generous shaking for 90 min. To derivatize hydroxyl amine groups to trimethylsilyated (TMS) forms, 80 µL of (MSTFA) with 1% trimethylchlorosilane (TMCS) were added to each vial, followed incubation at 37°C with shaking for 30 min. The samples were allowed to cool room temperature and were then analyzed by GC-MS in random order. For technical each of the derivatized samples was split into two different vials and analyzed","PROCESSING_METHOD":"Lysed via bead-beating","EXTRACTION_METHOD":"four volumes of chilled (-20°C) chloroform/methanol (2:1, v/v), and the phases after centrifugation were transferred to glass vials and dried in vacuo Thermo Scientific, Waltham, MA)","EXTRACT_ENRICHMENT":"dried in vacuo","EXTRACT_STORAGE":"dried in vacuo, stored at -80° C","SAMPLE_RESUSPENSION":"20 µL of methoxyamine in pyridine (30 mg/mL)","SAMPLE_DERIVATIZATION":"20 µL of methoxyamine in pyridine (30 mg/mL), 80 µL of (MSTFA) with 1% trimethylchlorosilane (TMCS)"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Agilent 7890A gas chromatograph with a HP-5MS gas chromatography column using","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent HP5-MS (30m × 0.25mm, 0.25 um)","CHROMATOGRAPHY_COMMENTS":"Chromatography was carried out on an Agilent 7890A gas chromatograph using the software (Chemstation) and a HP-5MS gas chromatography column (Agilent Santa Clara, CA; 30 m x 0.25 mm x 0.25 m film thickness). The sample injection was splitless, and 1 L of each sample was injected. The injection port was held at 250 C throughout the analysis. The GC oven was held at 60 C for 1 after injection, and the temperature was then increased to 325 C by 10 C/min, by a 5 min hold at 325 C. The helium gas flow rates for each Experiment were by the Agilent Retention Time Locking function based on analysis of deuterated acid and were in the range of 0.450.5 mL/min.","FLOW_RATE":"0.450.5 mL/min","INJECTION_TEMPERATURE":"250 C","SAMPLE_INJECTION":"1 L, splitless","ANALYTICAL_TIME":"37.5 min","OVEN_TEMPERATURE":"60 C for 1 min, then increased to 325 C by 10 C/min, followed by a 5 min hold 325 C","SAMPLE_SYRINGE_SIZE":"10 L"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Biological Separations & Mass Spectrometry group, Pacific Northwest National (www.omics.pnl.gov)","ACQUISITION_DATE":"2013","SOFTWARE_VERSION":"Metabolite Detector vs. 2.0.6 beta","DATA_FORMAT":"Raw .D.Zip; Processed .CDF"},

"MS":{"INSTRUMENT_NAME":"Agilent 5975C","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"After converting raw data to netCDF format, the data were processed by the software for peak deconvolution and chromatographic alignment. Retention (RI) were calculated based on the analysis of a mixture of fatty acid methyl (C8 - C30) (Agilent Technologies) as external retention time standards, then retention index information was subsequently applied to all experimental for retention time alignment. MetaboliteDetector parameters for peak detection deconvolution are as follows: Peak threshold, 7; minimum peak height, 7; width, 8. Deconvoluted features were identified by matching to the Agilent Metabolomics Retention Time Locked Library, which contains mass spectral and index information for approximately 700 metabolites. Each initial match to the was manually inspected to confirm a confident identification. software was used for database matching and batch identification/quantification are as follows: required score, 0.6; ?RI, 25; minimum S/N, 20; maximum peak index, 100. Ions 73 and 143 were excluded from use as metabolite quantification since these are due to fragmentation of the trimethylsilyl groups. Otherwise, unique fragment ions were assigned to each metabolite for quantification and for each individual GC-MS analysis when processing the data in batch mode. The areas of the three quantification ions were exported from MetaboliteDetector used in further statistical anayses. All identifications were manually by inspection of retention index and spectrum matches.","SCAN_RANGE_MOVERZ":"50-550 m/z"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"1_3-diaminopropane","SBEP_STM_LPM20_1":"-0.8151","SBEP_STM_LPM20_2":"-0.8151","SBEP_STM_LPM20_3":"-0.8151","SBEP_STM_LPM4_1":"-0.4872","SBEP_STM_LPM4_2":"-0.4165","SBEP_STM_LPM4_3":"-0.4080","SBEP_STM_LB_1":"0.5053","SBEP_STM_LB_2":"1.7966","SBEP_STM_LB_3":"1.4550"},{"Metabolite":"2_6-diaminopimelic acid","SBEP_STM_LPM20_1":"0.8067","SBEP_STM_LPM20_2":"0.4197","SBEP_STM_LPM20_3":"1.7093","SBEP_STM_LPM4_1":"-1.2563","SBEP_STM_LPM4_2":"-0.5660","SBEP_STM_LPM4_3":"","SBEP_STM_LB_1":"","SBEP_STM_LB_2":"-0.4104","SBEP_STM_LB_3":"-0.7029"},{"Metabolite":"4-guanidinobutyric acid","SBEP_STM_LPM20_1":"1.2770","SBEP_STM_LPM20_2":"0.2133","SBEP_STM_LPM20_3":"","SBEP_STM_LPM4_1":"-1.1369","SBEP_STM_LPM4_2":"1.5736","SBEP_STM_LPM4_3":"-0.5359","SBEP_STM_LB_1":"-0.9536","SBEP_STM_LB_2":"-0.6065","SBEP_STM_LB_3":"0.1691"},{"Metabolite":"4-hydroxyphenyllactic acid","SBEP_STM_LPM20_1":"0.8083","SBEP_STM_LPM20_2":"","SBEP_STM_LPM20_3":"0.7868","SBEP_STM_LPM4_1":"-0.3016","SBEP_STM_LPM4_2":"1.4041","SBEP_STM_LPM4_3":"0.5216","SBEP_STM_LB_1":"-1.2329","SBEP_STM_LB_2":"-0.9296","SBEP_STM_LB_3":"-1.0568"},{"Metabolite":"6-deoxy-D-glucose","SBEP_STM_LPM20_1":"0.4991","SBEP_STM_LPM20_2":"0.0379","SBEP_STM_LPM20_3":"1.9583","SBEP_STM_LPM4_1":"","SBEP_STM_LPM4_2":"-0.2122","SBEP_STM_LPM4_3":"-0.5355","SBEP_STM_LB_1":"-1.3416","SBEP_STM_LB_2":"-0.4060","SBEP_STM_LB_3":""},{"Metabolite":"adenine","SBEP_STM_LPM20_1":"0.6688","SBEP_STM_LPM20_2":"0.4967","SBEP_STM_LPM20_3":"0.7652","SBEP_STM_LPM4_1":"-1.4048","SBEP_STM_LPM4_2":"-1.1574","SBEP_STM_LPM4_3":"-1.1710","SBEP_STM_LB_1":"1.0892","SBEP_STM_LB_2":"1.0248","SBEP_STM_LB_3":"-0.3115"},{"Metabolite":"adenosine","SBEP_STM_LPM20_1":"1.4963","SBEP_STM_LPM20_2":"","SBEP_STM_LPM20_3":"0.9465","SBEP_STM_LPM4_1":"-0.9398","SBEP_STM_LPM4_2":"-1.0292","SBEP_STM_LPM4_3":"","SBEP_STM_LB_1":"-0.4634","SBEP_STM_LB_2":"0.7424","SBEP_STM_LB_3":"-0.7527"},{"Metabolite":"adenosine-5-monophosphate","SBEP_STM_LPM20_1":"0.2399","SBEP_STM_LPM20_2":"1.4880","SBEP_STM_LPM20_3":"0.7501","SBEP_STM_LPM4_1":"-0.8923","SBEP_STM_LPM4_2":"-1.4422","SBEP_STM_LPM4_3":"-1.0615","SBEP_STM_LB_1":"0.5478","SBEP_STM_LB_2":"0.9629","SBEP_STM_LB_3":"-0.5928"},{"Metabolite":"alpha-D-glucose","SBEP_STM_LPM20_1":"-0.0007","SBEP_STM_LPM20_2":"-0.1520","SBEP_STM_LPM20_3":"0.0029","SBEP_STM_LPM4_1":"0.2198","SBEP_STM_LPM4_2":"0.5450","SBEP_STM_LPM4_3":"2.1132","SBEP_STM_LB_1":"-0.9478","SBEP_STM_LB_2":"-0.8804","SBEP_STM_LB_3":"-0.9001"},{"Metabolite":"beta- alanine","SBEP_STM_LPM20_1":"-0.4220","SBEP_STM_LPM20_2":"-0.8393","SBEP_STM_LPM20_3":"-0.3217","SBEP_STM_LPM4_1":"-0.5981","SBEP_STM_LPM4_2":"-0.1294","SBEP_STM_LPM4_3":"-0.9882","SBEP_STM_LB_1":"-0.9960","SBEP_STM_LB_2":"1.4658","SBEP_STM_LB_3":"1.1790"},{"Metabolite":"citramalic acid","SBEP_STM_LPM20_1":"-0.0417","SBEP_STM_LPM20_2":"-0.2003","SBEP_STM_LPM20_3":"0.0523","SBEP_STM_LPM4_1":"0.0055","SBEP_STM_LPM4_2":"-0.7103","SBEP_STM_LPM4_3":"2.1107","SBEP_STM_LB_1":"-1.1688","SBEP_STM_LB_2":"0.2579","SBEP_STM_LB_3":"-0.3054"},{"Metabolite":"citrulline","SBEP_STM_LPM20_1":"-0.4958","SBEP_STM_LPM20_2":"1.3357","SBEP_STM_LPM20_3":"0.5173","SBEP_STM_LPM4_1":"-0.6108","SBEP_STM_LPM4_2":"-0.7972","SBEP_STM_LPM4_3":"-1.2357","SBEP_STM_LB_1":"-0.1762","SBEP_STM_LB_2":"0.7401","SBEP_STM_LB_3":"0.7226"},{"Metabolite":"cytosine","SBEP_STM_LPM20_1":"0.9691","SBEP_STM_LPM20_2":"1.0507","SBEP_STM_LPM20_3":"1.6213","SBEP_STM_LPM4_1":"-0.8693","SBEP_STM_LPM4_2":"","SBEP_STM_LPM4_3":"-0.8567","SBEP_STM_LB_1":"-0.4617","SBEP_STM_LB_2":"-0.5969","SBEP_STM_LB_3":"-0.8566"},{"Metabolite":"deoxycytidylic acid","SBEP_STM_LPM20_1":"0.8879","SBEP_STM_LPM20_2":"1.0751","SBEP_STM_LPM20_3":"1.4480","SBEP_STM_LPM4_1":"-1.3043","SBEP_STM_LPM4_2":"","SBEP_STM_LPM4_3":"-1.0120","SBEP_STM_LB_1":"-0.4173","SBEP_STM_LB_2":"0.0001","SBEP_STM_LB_3":"-0.6776"},{"Metabolite":"D-glucose-6-phosphate","SBEP_STM_LPM20_1":"-0.7211","SBEP_STM_LPM20_2":"-0.7547","SBEP_STM_LPM20_3":"-1.0250","SBEP_STM_LPM4_1":"-0.5527","SBEP_STM_LPM4_2":"-0.3395","SBEP_STM_LPM4_3":"-0.3500","SBEP_STM_LB_1":"0.8807","SBEP_STM_LB_2":"1.7747","SBEP_STM_LB_3":"1.0877"},{"Metabolite":"DIHYDROXYACETONE PHOSPHATE","SBEP_STM_LPM20_1":"-0.6143","SBEP_STM_LPM20_2":"-0.6143","SBEP_STM_LPM20_3":"-0.6143","SBEP_STM_LPM4_1":"-0.6143","SBEP_STM_LPM4_2":"-0.6143","SBEP_STM_LPM4_3":"-0.6143","SBEP_STM_LB_1":"1.4046","SBEP_STM_LB_2":"1.7228","SBEP_STM_LB_3":"0.5587"},{"Metabolite":"diphosphoric 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}