{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000110","ANALYSIS_ID":"AN000184","VERSION":"1","CREATED_ON":"2016-09-17"},
"PROJECT":{"PROJECT_TITLE":"SIRM Analysis of human P493","PROJECT_SUMMARY":"SIRM Analysis of human MYC-inducible P493 cells","INSTITUTE":"Johns Hopkins University","DEPARTMENT":"Pathology","LAST_NAME":"Dang","FIRST_NAME":"Chi","ADDRESS":"-","EMAIL":"cvdang@jhmi.edu","PHONE":"-"},
"STUDY":{"STUDY_TITLE":"SIRM Analysis of human P493 cells under hypoxia in [U-13C/15N] labeled medium (Both positive and ion mode FTMS)","STUDY_TYPE":"tracer","STUDY_SUMMARY":"SIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in labeled Glutamine medium","INSTITUTE":"University of Kentucky","DEPARTMENT":"Toxicology","LAST_NAME":"Fan","FIRST_NAME":"Teresa","ADDRESS":"-","EMAIL":"twmfan@gmail.com","PHONE":"-","NUM_GROUPS":"4","TOTAL_SUBJECTS":"8","STUDY_COMMENTS":"For protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., Dang, C.V. Cell Metabolism 15, 110 (2012)."},
"SUBJECT":{"SUBJECT_TYPE":"cell culture","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENOTYPE_STRAIN":"P493","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"hypox-13C/15N-Gln-plate-1",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"3.9000000000000146","dry_weight%units":"mg","pellet_weight":"44.69999999999996","pellet_weight%units":"mg","wet_weight":"0.21279999999999988","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"hypox-13C/15N-Gln-plate-2",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"3.7000000000000366","dry_weight%units":"mg","pellet_weight":"45.00000000000015","pellet_weight%units":"mg","wet_weight":"0.21100000000000008","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"tet-hypox-13C/15N-Gln-plate-1",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"4.1999999999999815","dry_weight%units":"mg","pellet_weight":"44.10000000000003","pellet_weight%units":"mg","wet_weight":"0.20619999999999994","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"tet-hypox-13C/15N-Gln-plate-2",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"3.3000000000000806","dry_weight%units":"mg","pellet_weight":"45.5000000000001","pellet_weight%units":"mg","wet_weight":"0.20974999999999988","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"normox-13C/15N-Gln-plate-1",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"3.7000000000000366","dry_weight%units":"mg","pellet_weight":"37.60000000000008","pellet_weight%units":"mg","wet_weight":"0.2197499999999999","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"normox-13C/15N-Gln-plate-2",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"4.0999999999999925","dry_weight%units":"mg","pellet_weight":"38.000000000000036","pellet_weight%units":"mg","wet_weight":"0.2125999999999999","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"tet-normox-13C/15N-Gln-plate-1",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"5.50000000000006","dry_weight%units":"mg","pellet_weight":"46.70000000000019","pellet_weight%units":"mg","wet_weight":"0.2137","wet_weight%units":"g"}
},
{
"Subject ID":"-",
"Sample ID":"tet-normox-13C/15N-Gln-plate-2",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"5.400000000000071","dry_weight%units":"mg","pellet_weight":"52.70000000000019","pellet_weight%units":"mg","wet_weight":"0.21950000000000003","wet_weight%units":"g"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","COLLECTION_PROTOCOL_COMMENTS":"Collection and quench of cells in tissue culture"},
"TREATMENT":{"TREATMENT_SUMMARY":"-","TREATMENT_PROTOCOL_COMMENTS":"hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for + 24hr with [U-13C/15N]-Gln; tet-normox-13C/15N-Gln: cell culture under with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln"},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_COMMENTS":"Protein extraction and quantification. |Preparation of polar samples for GCMS NMR. | Lipid extraction and preparation for FTMS."},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"RCSIRM"},
"MS":{"INSTRUMENT_NAME":"Thermo LTQ-FT","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"FTMS Analytical Experiment with detection of compounds with unique CHONPS formulas."},
"MS_METABOLITE_DATA":{
"Units":"Peak area",
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