{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000114","ANALYSIS_ID":"AN000194","VERSION":"1","CREATED_ON":"2016-09-17"},
"PROJECT":{"PROJECT_TITLE":"SIRM Analysis of human P493","PROJECT_SUMMARY":"SIRM Analysis of human MYC-inducible P493 cells","INSTITUTE":"Johns Hopkins University","DEPARTMENT":"Pathology","LAST_NAME":"Dang","FIRST_NAME":"Chi","ADDRESS":"-","EMAIL":"cvdang@jhmi.edu","PHONE":"-"},
"STUDY":{"STUDY_TITLE":"SIRM Analysis of human P493 cells under hypoxia in [U-13C] labeled Glucose","STUDY_TYPE":"tracer","STUDY_SUMMARY":"SIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in [U-13C] Glucose medium","INSTITUTE":"University of Kentucky","DEPARTMENT":"Toxicology","LAST_NAME":"Fan","FIRST_NAME":"Teresa","ADDRESS":"-","EMAIL":"twmfan@gmail.com","PHONE":"-","STUDY_COMMENTS":"For protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., Dang, C.V. Cell Metabolism 15, 110 (2012)."},
"SUBJECT":{"SUBJECT_TYPE":"cell culture","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENOTYPE_STRAIN":"P493","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"hypox-13C-Glc-plate-1",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"9.0000","dry_weight%units":"mg","pellet_weight":"84.5000","pellet_weight%units":"mg","wet_weight":"3.1000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"hypox-13C-Glc-plate-2",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"8.3000","dry_weight%units":"mg","pellet_weight":"79.2000","pellet_weight%units":"mg","wet_weight":"3.1000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"tet-hypox-13C-Glc-plate-1",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"8.8000","dry_weight%units":"mg","pellet_weight":"72.7000","pellet_weight%units":"mg","wet_weight":"2.3000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"tet-hypox-13C-Glc-plate-2",
"Factors":{"Oxygen Condition":"hypoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"6.7000","dry_weight%units":"mg","pellet_weight":"68.0000","pellet_weight%units":"mg","wet_weight":"2.8000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"normox-13C-Glc-plate-1",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"6.3000","dry_weight%units":"mg","pellet_weight":"68.1000","pellet_weight%units":"mg","wet_weight":"2.2000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"normox-13C-Glc-plate-2",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"None"},
"Additional sample data":{"dry_weight":"6.0000","dry_weight%units":"mg","pellet_weight":"68.8000","pellet_weight%units":"mg","wet_weight":"3.1000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"tet-normox-13C-Glc-plate-1",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"5.4000","dry_weight%units":"mg","pellet_weight":"52.4000","pellet_weight%units":"mg","wet_weight":"3.4000","wet_weight%units":"mg"}
},
{
"Subject ID":"-",
"Sample ID":"tet-normox-13C-Glc-plate-2",
"Factors":{"Oxygen Condition":"normoxia","Treatment":"Tetracycline"},
"Additional sample data":{"dry_weight":"4.6000","dry_weight%units":"mg","pellet_weight":"50.0000","pellet_weight%units":"mg","wet_weight":"2.5000","wet_weight%units":"mg"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","COLLECTION_PROTOCOL_COMMENTS":"Collection and quench of cells in tissue culture"},
"TREATMENT":{"TREATMENT_SUMMARY":"-","TREATMENT_PROTOCOL_COMMENTS":"tet-hypox-13C-Glc: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr 24hr with [U-13C]-Glc | tet-normox-13C-Glc: cell culture under normoxia with Tetracycline for 24hr + 24hr with [U-13C]-Glc | hypox-13C-Glc: cell culture hypoxia for 24hr + 24hr with [U-13C]-Glc | normox-13C-Glc: cell culture under for 24hr + 24hr with [U-13C]-Glc"},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_COMMENTS":"Lipid extraction and preparation for FTMS. | Protein extraction and | Preparation of polar samples for GCMS and NMR."},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"RCSIRM"},
"MS":{"INSTRUMENT_NAME":"Thermo ITQ","INSTRUMENT_TYPE":"GC-ITQ","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"GCMS Analytical Experiment with detection of compounds by comparison to Derivitization is done with MTBSTFA"},
"MS_METABOLITE_DATA":{
"Units":"normalized Peak area",
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