{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000115","ANALYSIS_ID":"AN000196","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Impact of insulin deprivation and treatment on sphingolipid distribution in muscle subcellular compartments of streptozotocin-diabetic C57Bl/6 mice.","PROJECT_TYPE":"Targeted metabolomics","PROJECT_SUMMARY":"Insulin deprivation in type 1 diabetes (T1D) individuals increases lipolysis plasma free fatty acids (FFA) concentration, which can stimulate synthesis of bioactive lipids such as ceramides (Cer) and long-chain fatty acid-CoAs Ceramide was shown to decrease muscle insulin sensitivity, and at mitochondrial it stimulates reactive oxygen species production. Here, we show that insulin in streptozotocin diabetic C57BL/6 mice increases quadriceps muscle Cer which was correlated with a concomitant decrease in the body fat and increased FFA, glycosylated hemoglobin level (%Hb A1c), and muscular LCFa-CoA content. alternations were accompanied by an increase in protein expression in LCFa-CoA Cer synthesis (FATP1/ACSVL5, CerS1, CerS5), a decrease in the expression of implicated in muscle insulin sensitivity (GLUT4, GYS1), and inhibition of signaling cascade by Akt? and GYS3? phosphorylation under acute insulin Both the content and composition of sarcoplasmic fraction sphingolipids were affected by insulin deprivation, whereas mitochondrial fraction sphingolipids stable. The observed effects of insulin deprivation were reversed, except for and composition of LCFa-CoA, CerS protein expression, GYS1 gene expression, and status of Akt and GYS3? when exogenous insulin was provided by subcutaneous implants. Principal component analysis and Pearson's correlation analysis close relationships between the features of the diabetic phenotype, the content LCFa-CoAs and Cers containing C18-fatty acids in sarcoplasm, but not in Insulin replacement did not completely rescue the phenotype, especially the content of LCFa-CoA, or proteins implicated in Cer synthesis and muscle sensitivity. These persistent changes might contribute to muscle insulin observed in T1D individuals.","INSTITUTE":"Mayo Clinic","DEPARTMENT":"Endocrinology","LABORATORY":"Dr. Sreekumaran Nair's lab","LAST_NAME":"Nair","FIRST_NAME":"Sreekumaran","ADDRESS":"-","EMAIL":"Dasari.Surendra@mayo.edu","PHONE":"-","FUNDING_SOURCE":"R01-DK-41973, UL1 TR000135, the David Murdock Dole Professorship (K. S. Nair), the Stephenson Fellowship (P. Zabielski).","PROJECT_COMMENTS":"24368672"},

"STUDY":{"STUDY_TITLE":"Impact of insulin deprivation and treatment on sphingolipid distribution in muscle subcellular compartments of streptozotocin-diabetic C57Bl/6 mice","STUDY_TYPE":"Insulin depravation","STUDY_SUMMARY":"Experiments were conducted using 13-wk-old male C57BL/6J mice (Jackson Bar Harbor, ME). Mice were housed individually with free access to water and (TD.10112; Harlan Laboratories, Indianapolis, IN), with a 12:12-h light-dark and temperature and humidity control. Mice were acclimated for 1 wk prior to beginning of the experiment. The protocol was approved by the Mayo Clinic Animal Care and Use Committee. Following a 6-h fast, mice were given injections of STZ (125 mg/kg; in sodium acetate buffer, pH = 4.5) (67). were repeated on the following day. Control animals received intraperitoneal of vehicle. Only mice that displayed blood glucose ?300 mg/dl and an increase blood ketones (both values by Precision Xtra glucometer; Abbott Laboratories, Park, IL), hyperphagia, and polyuria and were positive for urine glucose via dipstick (Uristix, Bayer, Pittsburgh, PA) on day 7 after the first STZ dose included in the experiment. Animals that were positive for STZ diabetes LinBit subcutaneous insulin implant (LinShin Canada, Toronto, ON, Canada) (79) pentobarbital sodium anesthesia (Nebutal, 40 mg/kg of body wt) according to the protocol. Each animal received two subcutaneous implants (total dose: 0.2 U/24 for >30 days, 10 U/kg for 20-g mice). Insulin treatment was continued for 3 wk. animals (C; n = 13) received blank implants. Diabetic control was confirmed by measurements of blood and urinary glucose. In some cases, when urine glucose present and blood glucose was >288 mg/dl, the animal received a third implant. insulin treatment was continued until initially lower plasma glucose content in animals reached control values. Three weeks following implantation, diabetic were divided randomly into diabetic-treated (D + I; n = 13) and (D ? I; n = 13) groups. Insulin implants were removed from the D ? I group pentobarbital anesthesia, which led to the return of the diabetic phenotype 24 h. Animals from the D + I group continued on insulin treatment (Fig. 1). At age of 18 wk, animals from all groups were analyzed for body composition by an Body Composition Analyzer (EchoMRI, Houston, TX) and euthanized by decapitation wk after the initial STZ or vehicle dose. Figure 1 depicts the timeline of the and blood glucose profiles for each experimental group. Additional animals were for estimation of skeletal muscle insulin sensitivity by acute insulin The mice were divided into the C (n = 6), D ? I (n = 7), and D + I (n = 7) and followed appropriate experimental treatment, except for acute insulin 10 min prior to euthanization by pentobarbital overdose. Figure 1 of the PDF of the article summarizes the study design","INSTITUTE":"Mayo Clinic","DEPARTMENT":"Endocrinology","LAST_NAME":"Nair","FIRST_NAME":"Sreekumaran","ADDRESS":"-","EMAIL":"Dasari.Surendra@mayo.edu","PHONE":"-","NUM_GROUPS":"3","TOTAL_SUBJECTS":"39"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","AGE_OR_AGE_RANGE":"13-wk-old","GENDER":"Male","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"C2",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C3",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C35",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C38",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C4",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C44",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C45",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C47",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C48",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C49",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C5",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C50",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C51",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"C6",
"Factors":{"Treatment":"control"}
},
{
"Subject ID":"-",
"Sample ID":"D+14",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+21",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+23",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+24",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+32",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+52",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+53",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+54",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+55",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+56",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+57",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+58",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D+70",
"Factors":{"Treatment":"diabetic treated"}
},
{
"Subject ID":"-",
"Sample ID":"D-25",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-28",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-31",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-33",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-36",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-39",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-40",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-41",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-42",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-43",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-60",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-61",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-63",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-64",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-67",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-68",
"Factors":{"Treatment":"diabetic untreated"}
},
{
"Subject ID":"-",
"Sample ID":"D-69",
"Factors":{"Treatment":"diabetic untreated"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Mitochondria were isolated from quadriceps muscle by differential as described previously (38). Briefly, quadriceps muscle samples were on ice using a motor-driven Potter-Elvehjem tissue grinder. After initial the supernatant containing the mitochondrial and sarcoplasmic fraction was to a chilled microcentrifuge tube and centrifuged at 10,000 g for 2 min to mitochondria. The supernatant containing sarcoplasmic fraction was frozen for analysis. Mitochondrial pellet was washed twice by resuspending/centrifugation finally suspended in a mitochondrial storage buffer. The levels of both the and sphingolipids in homogenates and various muscle fractions were normalized total protein content, as measured by 660 nm Protein Assay (Thermo Scientific; Protein Biology Products, Rockford, IL)."},

"TREATMENT":{"TREATMENT_SUMMARY":"Control/Diabetic; insulin treated/Diabetic; insulin deprived","TREATMENT_COMPOUND":"blank/Insulin/Insulin","TREATMENT_ROUTE":"Skin implants","ANIMAL_ANESTHESIA":"phenobarbital","ANIMAL_ENDP_EUTHANASIA":"5 weeks after treatment","ANIMAL_ENDP_TISSUE_COLL_LIST":"plasma, muscle, liver and skin"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Mitochondria were isolated from quadriceps muscle by differential as described previously (38). Briefly, quadriceps muscle samples were on ice using a motor-driven Potter-Elvehjem tissue grinder. After initial the supernatant containing the mitochondrial and sarcoplasmic fraction was to a chilled microcentrifuge tube and centrifuged at 10,000 g for 2 min to mitochondria. The supernatant containing sarcoplasmic fraction was frozen for analysis. Mitochondrial pellet was washed twice by resuspending/centrifugation finally suspended in a mitochondrial storage buffer. The levels of both the and sphingolipids in homogenates and various muscle fractions were normalized total protein content, as measured by 660 nm Protein Assay (Thermo Scientific; Protein Biology Products, Rockford, IL). / Plasma free fatty acid were measured by liquid chromatography/mass spectrometry (LC/MS), as described (51). Briefly, 50 ?l of plasma was spiked with heptadecanoate internal standard and analyzed with Applied Biosystems (Foster City, CA) API5000 mass coupled with a Cohesive (Franklin, MA) TX2 liquid chromatography system. of individual FFA was measured against a six-point standard curve prepared for analyte. Both the ISTD and individual FFA standard curves were prepared in 2% acid-free human albumin solution. All analytes were monitored as their [M ? H]? plasma LCFa-CoA esters were estimated using the LC-MS/MS method (9). After in the presence of internal standard (20 ng of heptadecanoyl-CoA), samples were by UHPLC-ESI-MS/MS operating in multiple reaction monitoring mode [Waters UHPLC, C8 UPLC BEH column 2.1 × 150 mm, 1.7 ?m (Waters, Milford, MA) and TSQ Ultra triple-quad mass spectrometer (Thermo Fisher Scientific, Waltham, MA)]. standard curves were prepared using chemicals from Avanti Polar Lipids.","SAMPLEPREP_PROTOCOL_FILENAME":"PMID-24368672-Zabielski-Nair-AJPEM-2014.pdf","SAMPLEPREP_PROTOCOL_COMMENTS":"Pubmed ID: 24368672"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Plasma free fatty acid concentrations were measured by liquid spectrometry (LC/MS), as described previously (51). Briefly, 50 ?l of plasma spiked with heptadecanoate internal standard (ISTD) and analyzed with Applied (Foster City, CA) API5000 mass spectrometer coupled with a Cohesive (Franklin, TX2 liquid chromatography system. Concentration of individual FFA was measured a six-point standard curve prepared for each analyte. Both the ISTD and FFA standard curves were prepared in 2% fatty acid-free human albumin solution. analytes were monitored as their [M ? H]? ions. LCFa-CoA esters were estimated the LC-MS/MS method (9). After extraction in the presence of internal standard ng of heptadecanoyl-CoA), samples were analyzed by UHPLC-ESI-MS/MS operating in reaction monitoring mode [Waters Acquity UHPLC, C8 UPLC BEH column 2.1 150 mm, ?m (Waters, Milford, MA) and TSQ Quantum Ultra triple-quad mass spectrometer Fisher Scientific, Waltham, MA)]. All standard curves were prepared using from Avanti Polar Lipids.","CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-","METHODS_FILENAME":"PMID-24368672-Zabielski-Nair-AJPEM-2014.pdf","CHROMATOGRAPHY_COMMENTS":"Pubmed ID: 24368672"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo TSQ Quantum Ultra","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"LCFa-CoA esters were estimated using the LC-MS/MS method (9). After extraction the presence of internal standard (20 ng of heptadecanoyl-CoA), samples were by UHPLC-ESI-MS/MS operating in multiple reaction monitoring mode [Waters UHPLC, C8 UPLC BEH column 2.1 × 150 mm, 1.7 um (Waters, Milford, MA) and TSQ Ultra triple-quad mass spectrometer (Thermo Fisher Scientific, Waltham, MA)]. standard curves were prepared using chemicals from Avanti Polar Lipids. UHPLC-ESI-MS/MS operating in multiple reaction monitoring mode [Waters Acquity C8 UPLC BEH column 2.1 × 150 mm, 1.7 ?m (Waters, Milford, MA) and TSQ Quantum triple-quad mass spectrometer (Thermo Fisher Scientific, Waltham, MA)]."},

"MS_METABOLITE_DATA":{
"Units":"uM",

"Data":[{"Metabolite":"CoA(C14:0)","C2":"5.2848","C35":"6.2778","C38":"4.9347","C4":"6.3311","C44":"5.2943","C45":"4.8208","C47":"4.7555","C48":"4.9267","C5":"5.0689","C51":"5.9069","C6":"4.8734","D+14":"5.0065","D+21":"7.7689","D+23":"5.6185","D+24":"4.7954","D+32":"4.7573","D+52":"4.8604","D+53":"5.1954","D+54":"6.5832","D+55":"5.1038","D+56":"6.1258","D+57":"5.0516","D+58":"5.0680","D+70":"4.7984","D-33":"11.6771","D-36":"7.9040","D-39":"10.5392","D-40":"9.1245","D-41":"11.8395","D-42":"7.2200","D-43":"6.1279","D-60":"7.9160","D-63":"7.1086","D-64":"16.7366","D-67":"7.0848","D-68":"9.9473","D-69":"8.3607"},{"Metabolite":"CoA(C16:0)","C2":"23.9899","C35":"23.1492","C38":"22.7642","C4":"29.8621","C44":"25.9300","C45":"21.6483","C47":"26.0008","C48":"21.6630","C5":"27.7154","C51":"22.3276","C6":"19.6078","D+14":"22.6803","D+21":"23.5973","D+23":"24.3506","D+24":"20.3009","D+32":"19.8533","D+52":"22.7783","D+53":"22.7070","D+54":"24.5314","D+55":"28.3529","D+56":"29.9417","D+57":"29.9987","D+58":"21.8870","D+70":"29.0076","D-33":"36.6983","D-36":"33.8463","D-39":"42.9457","D-40":"47.7846","D-41":"42.5615","D-42":"35.7478","D-43":"26.5678","D-60":"35.8500","D-63":"42.5596","D-64":"47.1494","D-67":"25.4268","D-68":"27.5228","D-69":"34.3775"},{"Metabolite":"CoA(C16:1)","C2":"6.8233","C35":"6.2519","C38":"5.0813","C4":"6.9984","C44":"4.5096","C45":"5.2159","C47":"5.3040","C48":"5.0678","C5":"6.6766","C51":"4.8775","C6":"5.3869","D+14":"6.0882","D+21":"8.9806","D+23":"7.6869","D+24":"6.9152","D+32":"5.0316","D+52":"5.3544","D+53":"5.5338","D+54":"5.2039","D+55":"6.3070","D+56":"5.2433","D+57":"5.2229","D+58":"5.1413","D+70":"4.9179","D-33":"16.1202","D-36":"10.6829","D-39":"14.2667","D-40":"10.0275","D-41":"12.1121","D-42":"15.4596","D-43":"15.4456","D-60":"15.2491","D-63":"12.9316","D-64":"11.3667","D-67":"15.3407","D-68":"13.7176","D-69":"10.7117"},{"Metabolite":"CoA(C18:0)","C2":"16.4007","C35":"15.1143","C38":"19.6168","C4":"18.9009","C44":"16.8359","C45":"19.3660","C47":"16.0700","C48":"17.7580","C5":"18.7419","C51":"16.5090","C6":"15.5846","D+14":"26.1486","D+21":"18.7576","D+23":"23.6485","D+24":"22.5114","D+32":"26.4127","D+52":"19.1982","D+53":"24.8529","D+54":"27.5744","D+55":"21.9703","D+56":"20.7842","D+57":"24.1803","D+58":"23.1946","D+70":"17.0495","D-33":"35.0515","D-36":"34.1142","D-39":"39.1733","D-40":"36.2577","D-41":"27.7970","D-42":"33.9040","D-43":"33.4642","D-60":"29.4441","D-63":"39.8497","D-64":"27.5128","D-67":"31.5765","D-68":"34.3647","D-69":"35.0375"},{"Metabolite":"CoA(C18:1)","C2":"127.0393","C35":"150.4921","C38":"153.8324","C4":"122.1919","C44":"153.5189","C45":"140.3683","C47":"196.1930","C48":"140.6318","C5":"199.5369","C51":"190.3457","C6":"135.5862","D+14":"153.1214","D+21":"238.6221","D+23":"195.4221","D+24":"243.4459","D+32":"248.0229","D+52":"250.1856","D+53":"279.1965","D+54":"267.2188","D+55":"225.8604","D+56":"315.1545","D+57":"199.7275","D+58":"211.2388","D+70":"244.8166","D-33":"339.4013","D-36":"365.4142","D-39":"397.6767","D-40":"348.1690","D-41":"316.9819","D-42":"317.8112","D-43":"397.5645","D-60":"303.3289","D-63":"382.1000","D-64":"330.7714","D-67":"300.7500","D-68":"331.9967","D-69":"369.8437"},{"Metabolite":"CoA(C18:2)","C2":"23.8905","C35":"18.5148","C38":"20.9020","C4":"24.6729","C44":"20.1013","C45":"18.3353","C47":"20.9066","C48":"19.6199","C5":"23.1227","C51":"23.6154","C6":"21.2328","D+14":"26.4506","D+21":"25.0762","D+23":"24.6886","D+24":"26.1773","D+32":"27.5672","D+52":"26.7162","D+53":"24.8892","D+54":"21.3818","D+55":"22.8363","D+56":"23.8613","D+57":"28.8370","D+58":"23.2404","D+70":"26.9156","D-33":"38.5613","D-36":"36.8439","D-39":"36.4021","D-40":"30.8827","D-41":"38.4368","D-42":"32.4936","D-43":"27.6908","D-60":"29.5947","D-63":"34.6330","D-64":"29.0255","D-67":"29.5366","D-68":"31.5836","D-69":"35.8978"},{"Metabolite":"CoA(C20:0)","C2":"8.3788","C35":"9.2092","C38":"8.9167","C4":"9.8753","C44":"7.6880","C45":"9.2592","C47":"8.4641","C48":"9.3432","C5":"7.0205","C51":"8.4396","C6":"8.0056","D+14":"8.3038","D+21":"9.4741","D+23":"7.6048","D+24":"7.5877","D+32":"10.2779","D+52":"8.8250","D+53":"11.0171","D+54":"8.9912","D+55":"9.8948","D+56":"10.9749","D+57":"10.2920","D+58":"9.1123","D+70":"11.8782","D-33":"12.1054","D-36":"13.4570","D-39":"15.2562","D-40":"9.5571","D-41":"10.4248","D-42":"15.2788","D-43":"9.5028","D-60":"9.1944","D-63":"19.7083","D-64":"12.5794","D-67":"15.4695","D-68":"12.3302","D-69":"13.1275"}],

"Metabolites":[{"Metabolite":"CoA(C14:0)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"},{"Metabolite":"CoA(C16:0)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"},{"Metabolite":"CoA(C16:1)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"},{"Metabolite":"CoA(C18:0)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"},{"Metabolite":"CoA(C18:1)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"},{"Metabolite":"CoA(C18:2)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"},{"Metabolite":"CoA(C20:0)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"MAYO_ID"}]
}

}