{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000116","ANALYSIS_ID":"AN000197","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Comparative metabolomics analysis of the key metabolic nodes in propionic acid in Propionibacterium acidipropionici","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"-","INSTITUTE":"Jiangnan University","DEPARTMENT":"Key Laboratory of Carbohydrate Chemistry and Biotechnology","LAST_NAME":"Guan","FIRST_NAME":"Ningzi","ADDRESS":"1800 Lihu Ave, Binhu, Wuxi, Jiangsu, China","EMAIL":"caimo-zi@163.com","PHONE":"-"},

"STUDY":{"STUDY_TITLE":"Comparative metabolomics analysis of the key metabolic nodes in propionic acid in Propionibacterium acidipropionici","STUDY_TYPE":"strains comparison","STUDY_SUMMARY":"The parental P. acidipropionici and its genome-shuffled mutant were compared metabolomics to find the key metabolic nodes influencing PA production.","INSTITUTE":"Jiangnan University","DEPARTMENT":"Key Laboratory of Carbohydrate Chemistry and Biotechnology","LAST_NAME":"Guan","FIRST_NAME":"Ningzi","ADDRESS":"1800 Lihu Ave, Binhu, Wuxi, Jiangsu, China","EMAIL":"caimo-zi@163.com","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"Cells","SUBJECT_SPECIES":"Propionibacterium acidipropionici","TAXONOMY_ID":"GCMCC1.2230 | WSH1105","GENOTYPE_STRAIN":"wild type mutant","SPECIES_GROUP":"Microorganism"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Late exponential phase-P1",
"Factors":{"Growth phase":"Late exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Late exponential phase-P2",
"Factors":{"Growth phase":"Late exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Late exponential phase-P3",
"Factors":{"Growth phase":"Late exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Late exponential phase-S1",
"Factors":{"Growth phase":"Late exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Late exponential phase-S2",
"Factors":{"Growth phase":"Late exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Late exponential phase-S3",
"Factors":{"Growth phase":"Late exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Middle exponential phase-P1",
"Factors":{"Growth phase":"Middle exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Middle exponential phase-P2",
"Factors":{"Growth phase":"Middle exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Middle exponential phase-P3",
"Factors":{"Growth phase":"Middle exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Middle exponential phase-S1",
"Factors":{"Growth phase":"Middle exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Middle exponential phase-S2",
"Factors":{"Growth phase":"Middle exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Middle exponential phase-S3",
"Factors":{"Growth phase":"Middle exponential phase"}
},
{
"Subject ID":"-",
"Sample ID":"Stationary phase-P1",
"Factors":{"Growth phase":"Stationary phase"}
},
{
"Subject ID":"-",
"Sample ID":"Stationary phase-P2",
"Factors":{"Growth phase":"Stationary phase"}
},
{
"Subject ID":"-",
"Sample ID":"Stationary phase-P3",
"Factors":{"Growth phase":"Stationary phase"}
},
{
"Subject ID":"-",
"Sample ID":"Stationary phase-S1",
"Factors":{"Growth phase":"Stationary phase"}
},
{
"Subject ID":"-",
"Sample ID":"Stationary phase-S2",
"Factors":{"Growth phase":"Stationary phase"}
},
{
"Subject ID":"-",
"Sample ID":"Stationary phase-S3",
"Factors":{"Growth phase":"Stationary phase"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"The cells were quenched by immediately adding a threefold volume of pre-chilled (v/v) methanol solution containing 70 mM 4-(2-hydroxyethyl)-1-piperazine acid, and pelleted in a centrifuge (4,000 × g, ?4°C, 10 min)","COLLECTION_PROTOCOL_FILENAME":"Protocol_Methods-G.docx","SAMPLE_TYPE":"cell","COLLECTION_TIME":"Samples were collected at the middle exponential phase, late exponential phase, the end of fermentation","STORAGE_CONDITIONS":"-80°C","COLLECTION_VIALS":"50mL centrifuge tube","STORAGE_VIALS":"50mL centrifuge tube","COLLECTION_TUBE_TEMP":"pre-chilled at -80°C","ADDITIVES":"pre-chilled 60% (v/v) methanol solution containing 70 mM ethanesulphonic acid"},

"TREATMENT":{"TREATMENT_SUMMARY":"-","CELL_STORAGE":"-80°C","CELL_GROWTH_CONTAINER":"250 mL anaerobic jars","CELL_INOC_PROC":"inoculated at 1%","CELL_MEDIA":"10 g/L yeast extract, 5 g/L tryptic soy broth, 1.5 g/L KH2PO4, 2.5 g/L K2HPO4","CELL_ENVIR_COND":"incubated at 32°C, anaerobic"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"After quenching at –40°C, the cells were pelleted in a centrifuge (4,000 × ?4°C, 10 min). The pellets were washed with methanol solution and resuspended 1 mL 35% perchloric acid to extract the metabolites from the cells. The mixture frozen in liquid nitrogen and thawed three times. The supernatant was by adding K2CO3 solution with an initial concentration of 5 M, and an extract all metabolites was collected via centrifugation at 10,000 × g for 5 min at","PROCESSING_METHOD":"Homogenization and solvent removal","PROCESSING_STORAGE_CONDITIONS":"on ice","EXTRACTION_METHOD":"35% perchloric acid and 5 M K2CO3","EXTRACT_STORAGE":"-80°C","CELL_TYPE":"wildtype cells, genome shffled cells"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"LC-MS","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"LCMS-IT-TOF","COLUMN_NAME":"Shim-Pack VP-ODS 150 L 2.0","COLUMN_PRESSURE":"18 MPa","COLUMN_TEMPERATURE":"40C","FLOW_GRADIENT":"2% to 60% B for 15 min, 60% to 76% B for 15 min, and 76% to 2% B for 5 min (A:1 ammonium formate; B: 80% methanol (v/v) containing 1 mM ammonium formate)","FLOW_RATE":"0.2 mL/min","RETENTION_TIME":"35 min","SAMPLE_INJECTION":"10 ?L","SOLVENT_A":"1 mM ammonium formate","SOLVENT_B":"80% methanol (v/v) containing 1 mM ammonium formate"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Key Laboratory of Industrial Biotechnology","ACQUISITION_PARAMETERS_FILE":"Protocol_Methods-G.docx","SOFTWARE_VERSION":"LCMSsolution","DETECTOR_TYPE":"UV"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Shimadzu LCMS-IT-TOF","INSTRUMENT_TYPE":"IT-TOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE/NEGATIVE","COLLISION_ENERGY":"0.4","COLLISION_GAS":"N2","DRY_GAS_FLOW":"1.5 L/min","DRY_GAS_TEMP":"350°C","MASS_ACCURACY":"5ppm"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"1-Aminocyclopropane-1-carboxylate","Late exponential phase-P1":"24000000.0000","Late exponential phase-P2":"22000000.0000","Late exponential phase-P3":"23000000.0000","Late exponential phase-S1":"21000000.0000","Late exponential phase-S2":"19000000.0000","Late exponential phase-S3":"22000000.0000","Middle exponential phase-P1":"90000000.0000","Middle exponential phase-P2":"96000000.0000","Middle exponential phase-P3":"92000000.0000","Middle exponential phase-S1":"60000000.0000","Middle exponential phase-S2":"61000000.0000","Middle exponential phase-S3":"63000000.0000","Stationary phase-P1":"40000000.0000","Stationary phase-P2":"44000000.0000","Stationary phase-P3":"40000000.0000","Stationary phase-S1":"17000000.0000","Stationary phase-S2":"21000000.0000","Stationary phase-S3":"19000000.0000"},{"Metabolite":"2_6-Diaminoheptanedioate","Late exponential phase-P1":"4000000.0000","Late exponential phase-P2":"3900000.0000","Late exponential phase-P3":"4100000.0000","Late exponential phase-S1":"3200000.0000","Late exponential phase-S2":"3500000.0000","Late exponential phase-S3":"3000000.0000","Middle exponential phase-P1":"2700000.0000","Middle exponential phase-P2":"3100000.0000","Middle exponential phase-P3":"2200000.0000","Middle exponential phase-S1":"17000000.0000","Middle exponential phase-S2":"11000000.0000","Middle exponential phase-S3":"11000000.0000","Stationary phase-P1":"4300000.0000","Stationary phase-P2":"4900000.0000","Stationary phase-P3":"4300000.0000","Stationary phase-S1":"2200000.0000","Stationary phase-S2":"2500000.0000","Stationary phase-S3":"2100000.0000"},{"Metabolite":"2-Aminomuconate","Late exponential phase-P1":"2400000.0000","Late exponential phase-P2":"2200000.0000","Late exponential phase-P3":"2200000.0000","Late exponential phase-S1":"1000000.0000","Late exponential phase-S2":"1000000.0000","Late exponential phase-S3":"1300000.0000","Middle exponential phase-P1":"1200000.0000","Middle exponential phase-P2":"1400000.0000","Middle exponential phase-P3":"1500000.0000","Middle exponential phase-S1":"1800000.0000","Middle exponential phase-S2":"2300000.0000","Middle exponential phase-S3":"2200000.0000","Stationary phase-P1":"2200000.0000","Stationary phase-P2":"2200000.0000","Stationary phase-P3":"2100000.0000","Stationary phase-S1":"2300000.0000","Stationary phase-S2":"2200000.0000","Stationary phase-S3":"2400000.0000"},{"Metabolite":"2-Aminophenol","Late exponential phase-P1":"3600000.0000","Late exponential phase-P2":"3600000.0000","Late exponential phase-P3":"3500000.0000","Late exponential phase-S1":"5100000.0000","Late exponential phase-S2":"5300000.0000","Late exponential phase-S3":"5000000.0000","Middle exponential phase-P1":"1500000.0000","Middle exponential phase-P2":"1700000.0000","Middle exponential phase-P3":"1400000.0000","Middle exponential phase-S1":"4700000.0000","Middle exponential phase-S2":"4300000.0000","Middle exponential phase-S3":"4700000.0000","Stationary phase-P1":"2300000.0000","Stationary phase-P2":"2000000.0000","Stationary phase-P3":"2300000.0000","Stationary phase-S1":"4900000.0000","Stationary phase-S2":"4700000.0000","Stationary phase-S3":"4700000.0000"},{"Metabolite":"2-Deoxy-ribose","Late exponential phase-P1":"3400000.0000","Late exponential phase-P2":"5400000.0000","Late exponential phase-P3":"2600000.0000","Late exponential phase-S1":"16000000.0000","Late exponential phase-S2":"31000000.0000","Late exponential phase-S3":"15000000.0000","Middle exponential phase-P1":"12000000.0000","Middle exponential phase-P2":"9000000.0000","Middle exponential phase-P3":"11000000.0000","Middle exponential phase-S1":"12000000.0000","Middle exponential phase-S2":"11000000.0000","Middle exponential phase-S3":"12000000.0000","Stationary phase-P1":"27000000.0000","Stationary phase-P2":"25000000.0000","Stationary phase-P3":"27000000.0000","Stationary phase-S1":"8800000.0000","Stationary phase-S2":"8600000.0000","Stationary phase-S3":"8900000.0000"},{"Metabolite":"2-Oxoadipate","Late exponential phase-P1":"19000000.0000","Late exponential phase-P2":"20000000.0000","Late exponential phase-P3":"20000000.0000","Late exponential phase-S1":"48000000.0000","Late exponential phase-S2":"47000000.0000","Late exponential phase-S3":"45000000.0000","Middle exponential phase-P1":"3400000.0000","Middle exponential phase-P2":"1600000.0000","Middle exponential phase-P3":"2700000.0000","Middle exponential phase-S1":"14000000.0000","Middle exponential phase-S2":"13000000.0000","Middle exponential phase-S3":"11000000.0000","Stationary phase-P1":"50000000.0000","Stationary phase-P2":"55000000.0000","Stationary phase-P3":"52000000.0000","Stationary phase-S1":"30000000.0000","Stationary phase-S2":"44000000.0000","Stationary phase-S3":"41000000.0000"},{"Metabolite":"2-Oxobutanoate","Late exponential phase-P1":"700000.0000","Late exponential phase-P2":"710000.0000","Late exponential phase-P3":"700000.0000","Late exponential phase-S1":"510000.0000","Late exponential phase-S2":"530000.0000","Late exponential phase-S3":"560000.0000","Middle exponential phase-P1":"770000.0000","Middle exponential phase-P2":"680000.0000","Middle exponential phase-P3":"510000.0000","Middle exponential phase-S1":"1200000.0000","Middle exponential phase-S2":"1100000.0000","Middle exponential phase-S3":"1100000.0000","Stationary phase-P1":"1200000.0000","Stationary phase-P2":"1100000.0000","Stationary phase-P3":"1400000.0000","Stationary phase-S1":"1000000.0000","Stationary phase-S2":"1000000.0000","Stationary phase-S3":"1300000.0000"},{"Metabolite":"2-Oxoglutarate","Late exponential phase-P1":"2200000.0000","Late exponential phase-P2":"2400000.0000","Late exponential phase-P3":"2200000.0000","Late exponential phase-S1":"3700000.0000","Late exponential phase-S2":"3400000.0000","Late exponential phase-S3":"3600000.0000","Middle exponential phase-P1":"680000.0000","Middle exponential phase-P2":"670000.0000","Middle exponential phase-P3":"710000.0000","Middle exponential phase-S1":"2500000.0000","Middle exponential phase-S2":"2300000.0000","Middle exponential phase-S3":"2100000.0000","Stationary phase-P1":"3000000.0000","Stationary phase-P2":"2900000.0000","Stationary phase-P3":"2700000.0000","Stationary phase-S1":"3200000.0000","Stationary phase-S2":"3600000.0000","Stationary phase-S3":"3500000.0000"},{"Metabolite":"3-Phospho-glycerate","Late exponential phase-P1":"570000.0000","Late exponential phase-P2":"700000.0000","Late exponential phase-P3":"770000.0000","Late exponential phase-S1":"8300000.0000","Late exponential phase-S2":"7500000.0000","Late exponential phase-S3":"6100000.0000","Middle exponential phase-P1":"720000.0000","Middle exponential phase-P2":"710000.0000","Middle exponential phase-P3":"770000.0000","Middle exponential phase-S1":"1700000.0000","Middle exponential phase-S2":"980000.0000","Middle exponential phase-S3":"1700000.0000","Stationary phase-P1":"2500000.0000","Stationary phase-P2":"1700000.0000","Stationary phase-P3":"2100000.0000","Stationary phase-S1":"1400000.0000","Stationary phase-S2":"1700000.0000","Stationary phase-S3":"1500000.0000"},{"Metabolite":"3-Phospho-glyceroyl phosphate","Late exponential phase-P1":"34000000.0000","Late exponential phase-P2":"35000000.0000","Late exponential phase-P3":"32000000.0000","Late exponential phase-S1":"3800000.0000","Late exponential phase-S2":"3400000.0000","Late exponential phase-S3":"3800000.0000","Middle exponential phase-P1":"75000000.0000","Middle exponential phase-P2":"74000000.0000","Middle exponential phase-P3":"67000000.0000","Middle exponential phase-S1":"3100000.0000","Middle exponential phase-S2":"3800000.0000","Middle exponential phase-S3":"2300000.0000","Stationary phase-P1":"22000000.0000","Stationary phase-P2":"24000000.0000","Stationary phase-P3":"25000000.0000","Stationary phase-S1":"18000000.0000","Stationary phase-S2":"17000000.0000","Stationary phase-S3":"17000000.0000"},{"Metabolite":"4-Aminobutyraldehyde","Late exponential phase-P1":"46000000.0000","Late exponential phase-P2":"44000000.0000","Late exponential phase-P3":"46000000.0000","Late exponential phase-S1":"38000000.0000","Late exponential phase-S2":"38000000.0000","Late exponential phase-S3":"36000000.0000","Middle exponential phase-P1":"28000000.0000","Middle exponential phase-P2":"27000000.0000","Middle exponential phase-P3":"28000000.0000","Middle exponential phase-S1":"21000000.0000","Middle exponential phase-S2":"22000000.0000","Middle exponential phase-S3":"19000000.0000","Stationary phase-P1":"57000000.0000","Stationary phase-P2":"55000000.0000","Stationary phase-P3":"58000000.0000","Stationary phase-S1":"48000000.0000","Stationary phase-S2":"46000000.0000","Stationary phase-S3":"48000000.0000"},{"Metabolite":"4-Hydroxybenzoate","Late exponential phase-P1":"13000000.0000","Late exponential phase-P2":"9600000.0000","Late exponential phase-P3":"8300000.0000","Late exponential phase-S1":"40000000.0000","Late exponential phase-S2":"38000000.0000","Late exponential phase-S3":"37000000.0000","Middle exponential phase-P1":"40000000.0000","Middle exponential phase-P2":"43000000.0000","Middle exponential phase-P3":"36000000.0000","Middle exponential phase-S1":"40000000.0000","Middle exponential phase-S2":"46000000.0000","Middle exponential phase-S3":"51000000.0000","Stationary phase-P1":"3200000.0000","Stationary phase-P2":"2200000.0000","Stationary phase-P3":"3000000.0000","Stationary phase-S1":"29000000.0000","Stationary phase-S2":"17000000.0000","Stationary phase-S3":"14000000.0000"},{"Metabolite":"5_6-Dihydrouracil","Late exponential phase-P1":"780000.0000","Late exponential phase-P2":"790000.0000","Late exponential phase-P3":"780000.0000","Late exponential phase-S1":"2000000.0000","Late exponential phase-S2":"1900000.0000","Late exponential phase-S3":"2000000.0000","Middle exponential phase-P1":"730000.0000","Middle exponential phase-P2":"710000.0000","Middle exponential phase-P3":"730000.0000","Middle exponential phase-S1":"1100000.0000","Middle exponential phase-S2":"1200000.0000","Middle exponential phase-S3":"1200000.0000","Stationary phase-P1":"730000.0000","Stationary phase-P2":"750000.0000","Stationary phase-P3":"750000.0000","Stationary phase-S1":"2100000.0000","Stationary phase-S2":"2300000.0000","Stationary phase-S3":"2100000.0000"},{"Metabolite":"5-Aminoimidazole","Late exponential phase-P1":"13000000.0000","Late exponential phase-P2":"13000000.0000","Late exponential phase-P3":"9000000.0000","Late exponential phase-S1":"13000000.0000","Late exponential phase-S2":"17000000.0000","Late exponential phase-S3":"16000000.0000","Middle exponential phase-P1":"1200000.0000","Middle exponential phase-P2":"2100000.0000","Middle exponential phase-P3":"1400000.0000","Middle exponential phase-S1":"8900000.0000","Middle exponential phase-S2":"5100000.0000","Middle exponential phase-S3":"7200000.0000","Stationary phase-P1":"9900000.0000","Stationary phase-P2":"12000000.0000","Stationary phase-P3":"7200000.0000","Stationary phase-S1":"9900000.0000","Stationary phase-S2":"8900000.0000","Stationary phase-S3":"11000000.0000"},{"Metabolite":"5-Hydroxy-tryptophan","Late exponential phase-P1":"3200000.0000","Late exponential phase-P2":"3000000.0000","Late exponential phase-P3":"3600000.0000","Late exponential phase-S1":"9000000.0000","Late exponential phase-S2":"9200000.0000","Late exponential phase-S3":"7300000.0000","Middle exponential phase-P1":"45000000.0000","Middle exponential phase-P2":"49000000.0000","Middle exponential phase-P3":"60000000.0000","Middle exponential phase-S1":"9700000.0000","Middle exponential phase-S2":"17000000.0000","Middle exponential phase-S3":"17000000.0000","Stationary phase-P1":"26000000.0000","Stationary phase-P2":"31000000.0000","Stationary phase-P3":"25000000.0000","Stationary phase-S1":"83000000.0000","Stationary phase-S2":"78000000.0000","Stationary phase-S3":"75000000.0000"},{"Metabolite":"5^-Methylthioadenosine","Late exponential phase-P1":"9100000.0000","Late exponential phase-P2":"13000000.0000","Late exponential phase-P3":"14000000.0000","Late exponential phase-S1":"8300000.0000","Late exponential phase-S2":"6600000.0000","Late exponential phase-S3":"8300000.0000","Middle exponential phase-P1":"4200000.0000","Middle exponential phase-P2":"1700000.0000","Middle exponential phase-P3":"2700000.0000","Middle exponential phase-S1":"11000000.0000","Middle exponential phase-S2":"17000000.0000","Middle exponential phase-S3":"16000000.0000","Stationary phase-P1":"21000000.0000","Stationary phase-P2":"22000000.0000","Stationary phase-P3":"19000000.0000","Stationary phase-S1":"14000000.0000","Stationary phase-S2":"11000000.0000","Stationary phase-S3":"11000000.0000"},{"Metabolite":"5-Phosphoribosylamine","Late exponential phase-P1":"1200000.0000","Late exponential phase-P2":"1500000.0000","Late exponential phase-P3":"1200000.0000","Late exponential phase-S1":"1800000.0000","Late exponential phase-S2":"1600000.0000","Late exponential phase-S3":"1900000.0000","Middle exponential phase-P1":"780000.0000","Middle exponential phase-P2":"790000.0000","Middle exponential phase-P3":"790000.0000","Middle exponential phase-S1":"1200000.0000","Middle exponential phase-S2":"990000.0000","Middle exponential phase-S3":"1000000.0000","Stationary phase-P1":"870000.0000","Stationary phase-P2":"860000.0000","Stationary phase-P3":"860000.0000","Stationary phase-S1":"1600000.0000","Stationary phase-S2":"1900000.0000","Stationary phase-S3":"1800000.0000"},{"Metabolite":"6-Phospho-gluconate","Late exponential phase-P1":"16000000.0000","Late exponential phase-P2":"13000000.0000","Late exponential phase-P3":"13000000.0000","Late exponential phase-S1":"7500000.0000","Late exponential phase-S2":"7300000.0000","Late exponential phase-S3":"7400000.0000","Middle exponential phase-P1":"9500000.0000","Middle exponential phase-P2":"9500000.0000","Middle exponential phase-P3":"9700000.0000","Middle exponential phase-S1":"5400000.0000","Middle exponential phase-S2":"5300000.0000","Middle exponential phase-S3":"5300000.0000","Stationary phase-P1":"29000000.0000","Stationary phase-P2":"34000000.0000","Stationary phase-P3":"29000000.0000","Stationary phase-S1":"3300000.0000","Stationary phase-S2":"3400000.0000","Stationary phase-S3":"3700000.0000"},{"Metabolite":"7_8-Diaminononanoate","Late exponential phase-P1":"2200000.0000","Late exponential phase-P2":"2000000.0000","Late exponential phase-P3":"1700000.0000","Late exponential phase-S1":"980000.0000","Late exponential phase-S2":"1100000.0000","Late exponential phase-S3":"1200000.0000","Middle exponential phase-P1":"4400000.0000","Middle exponential phase-P2":"4700000.0000","Middle exponential phase-P3":"4500000.0000","Middle exponential phase-S1":"76000000.0000","Middle exponential phase-S2":"74000000.0000","Middle exponential phase-S3":"80000000.0000","Stationary phase-P1":"79000000.0000","Stationary phase-P2":"75000000.0000","Stationary phase-P3":"72000000.0000","Stationary phase-S1":"45000000.0000","Stationary phase-S2":"56000000.0000","Stationary phase-S3":"49000000.0000"},{"Metabolite":"Acetate","Late exponential phase-P1":"18000000.0000","Late exponential phase-P2":"22000000.0000","Late exponential phase-P3":"18000000.0000","Late exponential phase-S1":"19000000.0000","Late exponential phase-S2":"11000000.0000","Late exponential phase-S3":"8400000.0000","Middle exponential phase-P1":"11000000.0000","Middle exponential phase-P2":"15000000.0000","Middle exponential phase-P3":"13000000.0000","Middle exponential phase-S1":"5400000.0000","Middle exponential phase-S2":"6000000.0000","Middle exponential phase-S3":"6700000.0000","Stationary phase-P1":"34000000.0000","Stationary phase-P2":"32000000.0000","Stationary phase-P3":"22000000.0000","Stationary phase-S1":"15000000.0000","Stationary phase-S2":"26000000.0000","Stationary phase-S3":"12000000.0000"},{"Metabolite":"Acetyl phosphate","Late exponential phase-P1":"1300000.0000","Late exponential phase-P2":"1600000.0000","Late exponential phase-P3":"1200000.0000","Late exponential phase-S1":"920000.0000","Late exponential phase-S2":"930000.0000","Late exponential phase-S3":"930000.0000","Middle exponential phase-P1":"2300000.0000","Middle exponential phase-P2":"2300000.0000","Middle exponential phase-P3":"2100000.0000","Middle exponential phase-S1":"810000.0000","Middle exponential phase-S2":"810000.0000","Middle exponential phase-S3":"840000.0000","Stationary phase-P1":"1600000.0000","Stationary phase-P2":"1800000.0000","Stationary phase-P3":"1900000.0000","Stationary phase-S1":"1200000.0000","Stationary phase-S2":"1300000.0000","Stationary phase-S3":"1200000.0000"},{"Metabolite":"Acrylamide","Late exponential phase-P1":"16000000.0000","Late exponential phase-P2":"14000000.0000","Late exponential phase-P3":"11000000.0000","Late exponential phase-S1":"1500000.0000","Late exponential phase-S2":"1700000.0000","Late exponential phase-S3":"1600000.0000","Middle exponential phase-P1":"11000000.0000","Middle exponential phase-P2":"13000000.0000","Middle exponential phase-P3":"15000000.0000","Middle exponential phase-S1":"18000000.0000","Middle exponential phase-S2":"14000000.0000","Middle exponential phase-S3":"8500000.0000","Stationary phase-P1":"24000000.0000","Stationary phase-P2":"32000000.0000","Stationary phase-P3":"26000000.0000","Stationary phase-S1":"15000000.0000","Stationary phase-S2":"16000000.0000","Stationary phase-S3":"9000000.0000"},{"Metabolite":"Acrylate","Late exponential phase-P1":"810000.0000","Late exponential phase-P2":"820000.0000","Late exponential phase-P3":"810000.0000","Late exponential phase-S1":"1100000.0000","Late exponential phase-S2":"1100000.0000","Late exponential phase-S3":"1300000.0000","Middle 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}