{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000170","ANALYSIS_ID":"AN000447","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Leukemia stem cells studies_dup","PROJECT_TYPE":"Glycolysis/TCA/Nucleotide analysis (tissue/cells)","PROJECT_SUMMARY":"Define the function of AMPK metabolic sensor in leukemia stem cells","INSTITUTE":"Baylor College of Medicine","DEPARTMENT":"Molecular and Human genetics","LABORATORY":"Nakada Lab","LAST_NAME":"Nakada","FIRST_NAME":"Daisuke","ADDRESS":"Houston, TX","EMAIL":"nakada@bcm.edu","PHONE":"713-798-1175"},

"STUDY":{"STUDY_TITLE":"13C mass isotopomer analysis (LCMS flux studies) MLL-AF9 (part III)","STUDY_TYPE":"Glycolysis/TCA/Nucleotide analysis (tissue/cells)","STUDY_SUMMARY":"How cancer cells adapt to metabolically adverse conditions in patients and to proliferate is a fundamental question in cancer biology. Here we show that protein kinase (AMPK), a metabolic checkpoint kinase, confers metabolic stress to leukemia-initiating cells (LICs) and promotes leukemogenesis. Upon dietary MLL-AF9-induced murine acute myeloid leukemia (AML) activated AMPK and leukemogenic potential. AMPK deletion significantly delayed leukemogenesis and LICs by reducing the expression of glucose transporter 1 (Glut1), compromising flux, and increasing oxidative stress and DNA damage. LICs were particularly on AMPK to suppress oxidative stress in the hypoglycemic bone marrow Strikingly, AMPK inhibition synergized with physiological metabolic stress by dietary restriction and profoundly suppressed leukemogenesis. Our results that AMPK protects LICs from metabolic stress and that combining AMPK with physiological metabolic stress potently suppresses AML by inducing stress and DNA damage.","INSTITUTE":"University of Michigan","DEPARTMENT":"Molecular and Human genetics (Baylor College of Medicine)","LABORATORY":"Nakada Lab","LAST_NAME":"Saitoh","FIRST_NAME":"Yusuke","ADDRESS":"Houston, TX","EMAIL":"araskind@med.umich.edu>","PHONE":"713-798-1175","NUM_GROUPS":"1","TOTAL_SUBJECTS":"9"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"S00017008",
"Factors":{"GENOTYPE":"AMPKαfl/fl","GROUP":"Bone marrow"}
},
{
"Subject ID":"-",
"Sample ID":"S00017010",
"Factors":{"GENOTYPE":"AMPKαfl/fl","GROUP":"Bone marrow"}
},
{
"Subject ID":"-",
"Sample ID":"S00017012",
"Factors":{"GENOTYPE":"AMPKαfl/fl","GROUP":"Bone marrow"}
},
{
"Subject ID":"-",
"Sample ID":"S00017009",
"Factors":{"GENOTYPE":"AMPKαfl/fl","GROUP":"Spleen"}
},
{
"Subject ID":"-",
"Sample ID":"S00017011",
"Factors":{"GENOTYPE":"AMPKαfl/fl","GROUP":"Spleen"}
},
{
"Subject ID":"-",
"Sample ID":"S00017013",
"Factors":{"GENOTYPE":"AMPKαfl/fl","GROUP":"Spleen"}
},
{
"Subject ID":"-",
"Sample ID":"S00017014",
"Factors":{"GENOTYPE":"AMPKαΔ/Δ","GROUP":"Bone marrow"}
},
{
"Subject ID":"-",
"Sample ID":"S00017015",
"Factors":{"GENOTYPE":"AMPKαΔ/Δ","GROUP":"Bone marrow"}
},
{
"Subject ID":"-",
"Sample ID":"S00017016",
"Factors":{"GENOTYPE":"AMPKαΔ/Δ","GROUP":"Bone marrow"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent 1260","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)","METHODS_ID":"AQM020","METHODS_FILENAME":"QTOF-002-HILIC-35min-1mm.m"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"MRC2 (University of Michigan)","ACQUISITION_PARAMETERS_FILE":"QTOF-002-HILIC-35min-1mm.m","PROCESSING_PARAMETERS_FILE":"EX00389-Flux-Quant-method.m"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6520 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"mM",

"Data":[{"Metabolite":"2 or 3-phosphoglycerate","S00017008":"0.2749","S00017010":"0.2178","S00017012":"0.1060","S00017009":"0.0784","S00017011":"","S00017013":"","S00017014":"0.0791","S00017015":"","S00017016":"0.0442"},{"Metabolite":"6-Phosphogluconate","S00017008":"0.0355","S00017010":"0.0303","S00017012":"0.0504","S00017009":"0.0209","S00017011":"0.0412","S00017013":"0.0522","S00017014":"0.0721","S00017015":"0.0286","S00017016":"0.0356"},{"Metabolite":"Acetyl-CoA","S00017008":"0.0540","S00017010":"0.0442","S00017012":"0.1001","S00017009":"0.0116","S00017011":"","S00017013":"0.0125","S00017014":"","S00017015":"","S00017016":""},{"Metabolite":"Adenosine diphosphate","S00017008":"3.2166","S00017010":"2.9132","S00017012":"3.3073","S00017009":"4.8869","S00017011":"2.6028","S00017013":"3.9940","S00017014":"1.9633","S00017015":"0.6554","S00017016":"1.0599"},{"Metabolite":"Adenosine monophosphate","S00017008":"0.9395","S00017010":"0.5836","S00017012":"1.2922","S00017009":"4.9193","S00017011":"5.0201","S00017013":"10.4192","S00017014":"2.8176","S00017015":"1.4996","S00017016":"0.9127"},{"Metabolite":"Adenosine triphosphate","S00017008":"1.7826","S00017010":"1.6929","S00017012":"1.6539","S00017009":"2.0781","S00017011":"1.2834","S00017013":"1.3987","S00017014":"1.7462","S00017015":"1.1473","S00017016":"1.2682"},{"Metabolite":"Citrate or Isocitrate","S00017008":"1.1254","S00017010":"0.9106","S00017012":"2.5543","S00017009":"0.7856","S00017011":"1.6035","S00017013":"2.7287","S00017014":"1.6892","S00017015":"1.0634","S00017016":"2.4373"},{"Metabolite":"Erythrose 4-phosphate","S00017008":"1.2759","S00017010":"0.6095","S00017012":"1.9125","S00017009":"0.5430","S00017011":"1.1120","S00017013":"3.9477","S00017014":"1.6767","S00017015":"1.6723","S00017016":"1.4242"},{"Metabolite":"Fructose 1_6-bisphosphate","S00017008":"0.8975","S00017010":"0.7937","S00017012":"6.2013","S00017009":"1.6072","S00017011":"1.5003","S00017013":"10.4554","S00017014":"1.6042","S00017015":"0.5498","S00017016":"0.5502"},{"Metabolite":"Malate","S00017008":"4.2773","S00017010":"4.6436","S00017012":"6.8832","S00017009":"3.4428","S00017011":"3.0444","S00017013":"3.1205","S00017014":"2.8250","S00017015":"0.3788","S00017016":"1.9067"},{"Metabolite":"Nicotinamide Adenine Dinucleotide oxidized","S00017008":"0.1260","S00017010":"0.0450","S00017012":"0.0191","S00017009":"0.0429","S00017011":"0.0146","S00017013":"0.0113","S00017014":"0.0224","S00017015":"0.0101","S00017016":"0.1229"},{"Metabolite":"Nicotinamide Adenine Dinucleotide reduced","S00017008":"9.7775","S00017010":"10.8438","S00017012":"8.3556","S00017009":"9.1727","S00017011":"","S00017013":"3.4163","S00017014":"2.8091","S00017015":"","S00017016":"2.0664"},{"Metabolite":"Phosphoenolpyruvate","S00017008":"","S00017010":"","S00017012":"0.1169","S00017009":"","S00017011":"","S00017013":"0.1560","S00017014":"","S00017015":"","S00017016":""},{"Metabolite":"Ribose-5-phosphate or xylulose 5-phosphate","S00017008":"1.8613","S00017010":"0.9631","S00017012":"1.5465","S00017009":"0.9984","S00017011":"2.3023","S00017013":"2.8097","S00017014":"1.5578","S00017015":"1.2378","S00017016":"2.0549"},{"Metabolite":"Sedoheptulose 7-phosphate","S00017008":"0.2601","S00017010":"0.1196","S00017012":"0.4332","S00017009":"0.1314","S00017011":"0.2188","S00017013":"0.5454","S00017014":"0.2105","S00017015":"0.1866","S00017016":"0.1462"},{"Metabolite":"Sucrose","S00017008":"","S00017010":"","S00017012":"","S00017009":"","S00017011":"","S00017013":"","S00017014":"","S00017015":"0.0019","S00017016":""}],

"Metabolites":[{"Metabolite":"2 or 3-phosphoglycerate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"724","inchi_key":"","kegg_id":"C00597","other_id":"2PG/3PG","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"6-Phosphogluconate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"91493","inchi_key":"","kegg_id":"C00345","other_id":"6PG","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Acetyl-CoA","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"444493","inchi_key":"","kegg_id":"C00024","other_id":"aCoA","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Adenosine diphosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6022","inchi_key":"","kegg_id":"C00008","other_id":"ADP","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Adenosine monophosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6083","inchi_key":"","kegg_id":"C00020","other_id":"AMP","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Adenosine triphosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5957","inchi_key":"","kegg_id":"C00002","other_id":"ATP","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Citrate or Isocitrate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"311","inchi_key":"","kegg_id":"C00158","other_id":"CIT/ICIT","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Erythrose 4-phosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"122357","inchi_key":"","kegg_id":"C00279","other_id":"E4P","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Fructose 1,6-bisphosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"172313","inchi_key":"","kegg_id":"C00354","other_id":"FBP","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Malate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"222656","inchi_key":"","kegg_id":"C00149","other_id":"MAL","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Nicotinamide Adenine Dinucleotide oxidized","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"10897651","inchi_key":"","kegg_id":"C00003","other_id":"NAD","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Nicotinamide Adenine Dinucleotide reduced","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"439153","inchi_key":"","kegg_id":"C00004","other_id":"NADH","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Phosphoenolpyruvate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1005","inchi_key":"","kegg_id":"C00074","other_id":"PEP","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Ribose-5-phosphate or xylulose 5-phosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"77982","inchi_key":"","kegg_id":"C00117","other_id":"R5P/X5P","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Sedoheptulose 7-phosphate","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"165007","inchi_key":"","kegg_id":"C05382","other_id":"S7P","other_id_type":"Nakada_Lab_ID"},{"Metabolite":"Sucrose","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5988","inchi_key":"","kegg_id":"C00089","other_id":"SUC","other_id_type":"Nakada_Lab_ID"}]
}

}