{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000185","ANALYSIS_ID":"AN000284","VERSION":"1","CREATED_ON":"01-15-2019"},

"PROJECT":{"PROJECT_TITLE":"LEC metabolomics","PROJECT_SUMMARY":"Comparison of metabolomic profile in LECs derived from normal and shunt animals","INSTITUTE":"University of California, San Francisco","DEPARTMENT":"Pediatrics","LABORATORY":"Fineman Lab","LAST_NAME":"Fineman","FIRST_NAME":"Jeffrey","ADDRESS":"San Francisco, CA","EMAIL":"jeff.fineman@ucsf.edu","PHONE":"415-502-6390","DOI":"http://dx.doi.org/10.21228/M8T01Q"},

"STUDY":{"STUDY_TITLE":"Fetal Lambs vascular graft Normal v Shunt LECs","STUDY_TYPE":"Glycolysis/TCA/Nucleotide analysis (tissue/cells)","STUDY_SUMMARY":"We recently reported superior right ventricle (RV) performance in response to acute afterload challenge in lambs with a model of congenital heart disease with chronic left-to-right cardiac shunts. Compared with control animals, shunt lambs demonstrated increased contractility because of an enhanced Anrep effect (the slow increase in contractility following myocyte stretch). This advantageous physiological response may reflect preservation of a fetal phenotype, since the RV of shunt lambs remains exposed to increased pressure postnatally. Nitric oxide (NO) production by NO synthase (NOS) is activated by myocyte stretch and is a necessary intermediary of the Anrep response. The purpose of this study was to test the hypothesis that NO signaling is increased in the RV of fetal lambs compared with controls and shunt lambs have persistence of this fetal pattern. An 8-mm graft was placed between the pulmonary artery and aorta in fetal lambs (shunt). NOS isoform expression, activity, and association with activating cofactors were determined in fetal tissue obtained during late-gestation and in 4-wk-old juvenile shunt and control lambs. We demonstrated increased RNA and protein expression of NOS isoforms and increased total NOS activity in the RV of both shunt and fetal lambs compared with control. We also found increased NOS activation and association with cofactors in shunt and fetal RV compared with control. These data demonstrate preserved fetal NOS phenotype and NO signaling in shunt RV, which may partially explain the mechanism underlying the adaptive response to increased afterload seen in the RV of shunt lambs. Research is published, core data not used but project description is relevant: http://ajpheart.physiology.org/content/309/1/H157.long","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@umich.edu","PHONE":"-"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Ovis aries","TAXONOMY_ID":"9940","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0011546",
"Sample ID":"S00017020",
"Factors":{"Type":"increased flow"}
},
{
"Subject ID":"SU0011547",
"Sample ID":"S00017021",
"Factors":{"Type":"increased flow"}
},
{
"Subject ID":"SU0011548",
"Sample ID":"S00017022",
"Factors":{"Type":"increased flow"}
},
{
"Subject ID":"SU0011543",
"Sample ID":"S00017017",
"Factors":{"Type":"normal control"}
},
{
"Subject ID":"SU0011544",
"Sample ID":"S00017018",
"Factors":{"Type":"normal control"}
},
{
"Subject ID":"SU0011545",
"Sample ID":"S00017019",
"Factors":{"Type":"normal control"}
}
],
"COLLECTION":{"SAMPLE_TYPE":"Endothelial Cells","COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_PROTOCOL_FILENAME":"Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx","SAMPLEPREP_SUMMARY":"-"},

"CHROMATOGRAPHY":{"METHODS_ID":"AQM020","METHODS_FILENAME":"QTOF-002-HILIC-35min-1mm-No_Insert.m.zip","INSTRUMENT_NAME":"Agilent 1260","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)","CHROMATOGRAPHY_TYPE":"HILIC"},

"ANALYSIS":{"LABORATORY_NAME":"MRC2 (University of Michigan)","ANALYSIS_TYPE":"MS","ACQUISITION_PARAMETERS_FILE":"QTOF-002-HILIC-35min-1mm-No_Insert.m.zip"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6530 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/µg protein",

"Data":[{"Metabolite":"2 or 3-phosphoglycerate_2PG/3PG","S00017020":"0.8978","S00017021":"0.3447","S00017022":"0.2680","S00017017":"6.4525","S00017018":"2.9002","S00017019":"6.3058"},{"Metabolite":"6-Phosphogluconate","S00017020":"0.6816","S00017021":"0.5188","S00017022":"0.3125","S00017017":"1.9113","S00017018":"1.4771","S00017019":"3.7586"},{"Metabolite":"Acetyl-CoA","S00017020":"0.1221","S00017021":"","S00017022":"","S00017017":"0.7683","S00017018":"0.5710","S00017019":"0.8624"},{"Metabolite":"ADP","S00017020":"21.4299","S00017021":"16.3787","S00017022":"15.1622","S00017017":"67.1431","S00017018":"40.6175","S00017019":"51.8677"},{"Metabolite":"AMP","S00017020":"9.3438","S00017021":"7.0078","S00017022":"7.4143","S00017017":"178.2130","S00017018":"68.4896","S00017019":"122.5572"},{"Metabolite":"ATP","S00017020":"66.8491","S00017021":"45.4450","S00017022":"39.1587","S00017017":"81.5229","S00017018":"90.1356","S00017019":"68.5431"},{"Metabolite":"Citrate or Isocitrate","S00017020":"30.3007","S00017021":"77.3516","S00017022":"33.7218","S00017017":"208.7459","S00017018":"129.0972","S00017019":"71.8109"},{"Metabolite":"flavin adenine dinucleotide","S00017020":"0.0975","S00017021":"0.1405","S00017022":"0.1166","S00017017":"0.3954","S00017018":"0.2555","S00017019":"0.3425"},{"Metabolite":"Fructose 1_6-bisphosphate","S00017020":"3.4687","S00017021":"1.1181","S00017022":"1.1003","S00017017":"16.3895","S00017018":"9.1119","S00017019":"15.0493"},{"Metabolite":"Glucose 6-phosphate or fructose-6-phosphate","S00017020":"16.2806","S00017021":"11.1167","S00017022":"7.8941","S00017017":"137.8086","S00017018":"33.0055","S00017019":"107.1428"},{"Metabolite":"Malate","S00017020":"42.5975","S00017021":"95.4928","S00017022":"36.2395","S00017017":"44.2528","S00017018":"36.1339","S00017019":""},{"Metabolite":"nicotinamide adenine dinucleotide phosphate oxidized","S00017020":"1.2664","S00017021":"1.1893","S00017022":"1.0154","S00017017":"3.6640","S00017018":"2.3189","S00017019":"3.4695"},{"Metabolite":"Phosphoenolpyruvate","S00017020":"1.7034","S00017021":"3.3100","S00017022":"0.9094","S00017017":"6.3348","S00017018":"7.7797","S00017019":"4.6110"},{"Metabolite":"Ribose-5-phosphate or xylulose 5-phosphate","S00017020":"4.0956","S00017021":"1.1797","S00017022":"1.5413","S00017017":"11.6761","S00017018":"4.3669","S00017019":"8.9582"}],

"Metabolites":[{"Metabolite":"2 or 3-phosphoglycerate_2PG/3PG","pubchem_id":"724","inchi_key":"","kegg_id":"C00597","other_id":"2PG/3PG","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"6-Phosphogluconate","pubchem_id":"91493","inchi_key":"","kegg_id":"C00345","other_id":"6PG","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Acetyl-CoA","pubchem_id":"444493","inchi_key":"","kegg_id":"C00024","other_id":"aCoA","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"ADP","pubchem_id":"6022","inchi_key":"","kegg_id":"C00008","other_id":"ADP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"AMP","pubchem_id":"6083","inchi_key":"","kegg_id":"C00020","other_id":"AMP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"ATP","pubchem_id":"5957","inchi_key":"","kegg_id":"C00002","other_id":"ATP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Citrate or Isocitrate","pubchem_id":"311","inchi_key":"","kegg_id":"C00158","other_id":"CIT/ICIT","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"flavin adenine dinucleotide","pubchem_id":"643975","inchi_key":"","kegg_id":"C00016","other_id":"FAD","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Fructose 1,6-bisphosphate","pubchem_id":"172313","inchi_key":"","kegg_id":"C00354","other_id":"FBP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Glucose 6-phosphate or fructose-6-phosphate","pubchem_id":"5958","inchi_key":"","kegg_id":"C00092","other_id":"G6P/F6P","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Malate","pubchem_id":"222656","inchi_key":"","kegg_id":"C00149","other_id":"MAL","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"nicotinamide adenine dinucleotide phosphate oxidized","pubchem_id":"5886","inchi_key":"","kegg_id":"C00006","other_id":"NADP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Phosphoenolpyruvate","pubchem_id":"1005","inchi_key":"","kegg_id":"C00074","other_id":"PEP","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""},{"Metabolite":"Ribose-5-phosphate or xylulose 5-phosphate","pubchem_id":"77982","inchi_key":"","kegg_id":"C00117","other_id":"R5P/X5P","other_id_type":"UM_Target_ID","ri":"","ri_type":"","moverz_quant":"","":""}]
}

}