{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000226","ANALYSIS_ID":"AN000336","VERSION":"1","CREATED_ON":"10-02-2015"},

"PROJECT":{"PROJECT_TITLE":"Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice","INSTITUTE":"Pennsylvania State University","DEPARTMENT":"Molecular Toxicology","LAST_NAME":"Andrew","FIRST_NAME":"Patterson","EMAIL":"adp117@PSU.EDU, RGN5011@psu.edu","PROJECT_SUMMARY":"-","PHONE":"-","ADDRESS":"-"},

"STUDY":{"STUDY_TITLE":"Metabolomics Reveals that Aryl Hydrocarbon Receptor Activation by Environmental Chemicals Induces Systemic Metabolic Dysfunction in Mice (Part I)","STUDY_TYPE":"drug dosage","STUDY_SUMMARY":"12 mice were acclimatized for a week, taught to eat dough pills then half were treated with TCDF per pill per day for 5 days Also 10 AHR -/- mice were used and treated the same way","INSTITUTE":"Pennsylvania State University","DEPARTMENT":"Molecular Toxicology","LAST_NAME":"Patterson","FIRST_NAME":"Andrew","EMAIL":"adp117@PSU.EDU","PHONE":"-","ADDRESS":"-"},

"SUBJECT":{"SUBJECT_TYPE":"mouse","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J; Wild Type and Ahr-/-","AGE_OR_AGE_RANGE":"6 weeks old","GENDER":"Males"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"Treated serum_Ahr-",
"Factors":{"Gender":"Male","Genotype":"Ahr -/-","Treatment":"TCDF"}
},
{
"Subject ID":"-",
"Sample ID":"Treated Liver",
"Factors":{"Gender":"Male","Genotype":"Ahr +/+","Treatment":"TCDF"}
},
{
"Subject ID":"-",
"Sample ID":"Treated serum_Ahr+",
"Factors":{"Gender":"Male","Genotype":"Ahr +/+","Treatment":"TCDF"}
},
{
"Subject ID":"-",
"Sample ID":"Control serum_Ahr-",
"Factors":{"Gender":"Male","Genotype":"Ahr -/-","Treatment":"Vehicle"}
},
{
"Subject ID":"-",
"Sample ID":"Control Liver",
"Factors":{"Gender":"Male","Genotype":"Ahr +/+","Treatment":"Vehicle"}
},
{
"Subject ID":"-",
"Sample ID":"Control serum_Ahr+",
"Factors":{"Gender":"Male","Genotype":"Ahr +/+","Treatment":"Vehicle"}
}
],
"COLLECTION":{"SAMPLE_TYPE":"Feces, liver, cecal contents, intestine and blood (only liver and blood tested with NRM)","COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"Wild type and AHR knock out mice (12 wild types and 10 AHR knock out) were treated with TCDF for 5 days","TREATMENT":"Dietary","TREATMENT_COMPOUND":"TCDF","TREATMENT_ROUTE":"Oral","TREATMENT_DOSE":"24 µg/kg body weight for AHR and 5 µg/kg body weight  for wild type"},

"SAMPLEPREP":{"SAMPLEPREP_PROTOCOL_FILENAME":"NMR_metabolomics_protocol.docx","PROCESSING_METHOD":"Homogenization","PROCESSING_STORAGE_CONDITIONS":"-20","EXTRACTION_METHOD":"Methanol/Water","SAMPLE_RESUSPENSION":"D2O","SAMPLE_SPIKING":"TSP-d4","ORGAN":"Liver, small and large intestine","SAMPLEPREP_SUMMARY":"-"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR"},

"NM":{"INSTRUMENT_TYPE":"Bruker Avance III 600 MHz spectrometer","NMR_EXPERIMENT_TYPE":"NOESY PR1D","SPECTROMETER_FREQUENCY":"600 MHz","NMR_SOLVENT":"D2O","INSTRUMENT_NAME":"Bruker Avance III"}

}