{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000249","ANALYSIS_ID":"AN000393","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Myc-directed suppression of autophagy provides therapeutic vulnerabilities amino acid homeostasis","PROJECT_TYPE":"Targeted LCMS Metabolomics","PROJECT_SUMMARY":"Hypothesis:MYC is suppressing the autophagy pathway which leads to impaired of proteins and reduced free AA in the cells.","INSTITUTE":"Moffitt Cancer Center","DEPARTMENT":"Tumor Biology","LABORATORY":"John Cleveland","LAST_NAME":"Cleveland","FIRST_NAME":"John","ADDRESS":"12902 Magnolia Dr","EMAIL":"Tampa, FL 33612","PHONE":"813 745 3888","FUNDING_SOURCE":"NIH"},

"STUDY":{"STUDY_TITLE":"Measurement of free amino acid (AA) in response to MYC","STUDY_TYPE":"treatment","STUDY_SUMMARY":"The proposed study will provide us information how the different AAs are by MYC. MYC is known to cause increased levels of glutamin due to import and but we hypothesize that the overall pool of free AA in the cells is reduced, when cells are starved.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LAST_NAME":"Schaub","FIRST_NAME":"Franz","ADDRESS":"-","EMAIL":"Franz.Schaub@moffitt.org","PHONE":"-","NUM_GROUPS":"2","TOTAL_SUBJECTS":"6"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"48hr_TET_1",
"Factors":{"Treatment":"48 hour Tet treatment"},
"Additional sample data":{"sample type":"Cell pellet from P493-6 B Cells"}
},
{
"Subject ID":"-",
"Sample ID":"48hr_TET_2",
"Factors":{"Treatment":"48 hour Tet treatment"},
"Additional sample data":{"sample type":"Cell pellet from P493-6 B Cells"}
},
{
"Subject ID":"-",
"Sample ID":"48hr_TET_3",
"Factors":{"Treatment":"48 hour Tet treatment"},
"Additional sample data":{"sample type":"Cell pellet from P493-6 B Cells"}
},
{
"Subject ID":"-",
"Sample ID":"48hr_NoTET_1",
"Factors":{"Treatment":"No Tet treatment"},
"Additional sample data":{"sample type":"Cell pellet from P493-6 B Cells"}
},
{
"Subject ID":"-",
"Sample ID":"48hr_NoTET_2",
"Factors":{"Treatment":"No Tet treatment"},
"Additional sample data":{"sample type":"Cell pellet from P493-6 B Cells"}
},
{
"Subject ID":"-",
"Sample ID":"48hr_NoTET_3",
"Factors":{"Treatment":"No Tet treatment"},
"Additional sample data":{"sample type":"Cell pellet from P493-6 B Cells"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were counted, washed with cold PBS and then flash-frozen in liquid N2","SAMPLE_TYPE":"Cells","VOLUMEORAMOUNT_COLLECTED":"1.00E7"},

"TREATMENT":{"TREATMENT_SUMMARY":"Cells were treated with TET for 48 hrs (TET samples) and then washed and without TET for 48hrs (noTET samples)"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Amino acid","SAMPLEPREP_PROTOCOL_FILENAME":"SB_AA_Assay.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Amino Acid","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 6490 QQQ","COLUMN_NAME":"Waters AccQ Tag (100 x 2mm, 1.7um)","METHODS_FILENAME":"SB_AA_Assay.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Sanford Burnham","DETECTOR_TYPE":"Triple Quadrupole"},

"MS":{"INSTRUMENT_NAME":"Agilent 6490 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Amino Acids"},

"MS_METABOLITE_DATA":{
"Units":"nmol/million cells",

"Data":[{"Metabolite":"Alanine","48hr_TET_1":"0.3090","48hr_TET_2":"0.2532","48hr_TET_3":"0.3071","48hr_NoTET_1":"0.3312","48hr_NoTET_2":"0.4396","48hr_NoTET_3":"0.4614"},{"Metabolite":"Arginine","48hr_TET_1":"0.1119","48hr_TET_2":"0.0880","48hr_TET_3":"0.0752","48hr_NoTET_1":"0.1316","48hr_NoTET_2":"0.1519","48hr_NoTET_3":"0.1505"},{"Metabolite":"Asparagine","48hr_TET_1":"0.3839","48hr_TET_2":"0.3703","48hr_TET_3":"0.3897","48hr_NoTET_1":"0.6176","48hr_NoTET_2":"0.7081","48hr_NoTET_3":"0.7002"},{"Metabolite":"Aspartic Acid","48hr_TET_1":"1.8494","48hr_TET_2":"1.6285","48hr_TET_3":"1.6821","48hr_NoTET_1":"1.3932","48hr_NoTET_2":"1.7418","48hr_NoTET_3":"1.6675"},{"Metabolite":"Citrulline","48hr_TET_1":"","48hr_TET_2":"","48hr_TET_3":"","48hr_NoTET_1":"","48hr_NoTET_2":"","48hr_NoTET_3":""},{"Metabolite":"Glutamic Acid","48hr_TET_1":"9.7901","48hr_TET_2":"8.9728","48hr_TET_3":"9.5972","48hr_NoTET_1":"9.8469","48hr_NoTET_2":"11.9768","48hr_NoTET_3":"10.2894"},{"Metabolite":"Glutamine","48hr_TET_1":"2.3819","48hr_TET_2":"2.0457","48hr_TET_3":"2.1411","48hr_NoTET_1":"2.5200","48hr_NoTET_2":"3.3155","48hr_NoTET_3":"3.4919"},{"Metabolite":"Glycine","48hr_TET_1":"1.4412","48hr_TET_2":"1.2484","48hr_TET_3":"1.1886","48hr_NoTET_1":"0.7270","48hr_NoTET_2":"1.0621","48hr_NoTET_3":"0.9695"},{"Metabolite":"Histidine","48hr_TET_1":"0.0695","48hr_TET_2":"0.0579","48hr_TET_3":"0.0615","48hr_NoTET_1":"0.0496","48hr_NoTET_2":"0.0784","48hr_NoTET_3":"0.0662"},{"Metabolite":"Isoleucine","48hr_TET_1":"0.1405","48hr_TET_2":"0.1207","48hr_TET_3":"0.1479","48hr_NoTET_1":"0.1329","48hr_NoTET_2":"0.1471","48hr_NoTET_3":"0.1665"},{"Metabolite":"Leucine","48hr_TET_1":"0.1531","48hr_TET_2":"0.1285","48hr_TET_3":"0.1525","48hr_NoTET_1":"0.1254","48hr_NoTET_2":"0.1441","48hr_NoTET_3":"0.1486"},{"Metabolite":"Lysine","48hr_TET_1":"","48hr_TET_2":"","48hr_TET_3":"","48hr_NoTET_1":"","48hr_NoTET_2":"","48hr_NoTET_3":""},{"Metabolite":"Methionine","48hr_TET_1":"0.0790","48hr_TET_2":"0.0780","48hr_TET_3":"0.0770","48hr_NoTET_1":"0.0695","48hr_NoTET_2":"0.0801","48hr_NoTET_3":"0.0772"},{"Metabolite":"Ornithine","48hr_TET_1":"","48hr_TET_2":"","48hr_TET_3":"","48hr_NoTET_1":"","48hr_NoTET_2":"","48hr_NoTET_3":""},{"Metabolite":"Phenylalanine","48hr_TET_1":"0.0557","48hr_TET_2":"0.0480","48hr_TET_3":"0.0552","48hr_NoTET_1":"0.0466","48hr_NoTET_2":"0.0598","48hr_NoTET_3":"0.0532"},{"Metabolite":"Proline","48hr_TET_1":"0.4964","48hr_TET_2":"0.4306","48hr_TET_3":"0.4714","48hr_NoTET_1":"1.0962","48hr_NoTET_2":"1.3621","48hr_NoTET_3":"1.4309"},{"Metabolite":"Serine","48hr_TET_1":"0.1952","48hr_TET_2":"0.1563","48hr_TET_3":"0.1813","48hr_NoTET_1":"0.1547","48hr_NoTET_2":"0.2017","48hr_NoTET_3":"0.2162"},{"Metabolite":"Threonine","48hr_TET_1":"0.1525","48hr_TET_2":"0.1243","48hr_TET_3":"0.1413","48hr_NoTET_1":"0.1462","48hr_NoTET_2":"0.2107","48hr_NoTET_3":"0.1861"},{"Metabolite":"Tryptophan","48hr_TET_1":"0.0120","48hr_TET_2":"0.0098","48hr_TET_3":"0.0129","48hr_NoTET_1":"0.0092","48hr_NoTET_2":"0.0127","48hr_NoTET_3":"0.0124"},{"Metabolite":"Tyrosine","48hr_TET_1":"0.0672","48hr_TET_2":"0.0652","48hr_TET_3":"0.0697","48hr_NoTET_1":"0.0670","48hr_NoTET_2":"0.0753","48hr_NoTET_3":"0.0756"},{"Metabolite":"Valine","48hr_TET_1":"0.0840","48hr_TET_2":"0.0796","48hr_TET_3":"0.0787","48hr_NoTET_1":"0.0536","48hr_NoTET_2":"0.0692","48hr_NoTET_3":"0.0650"}],

"Metabolites":[{"Metabolite":"Alanine","moverz_quant":"260.17, 171","ri":"4.16","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5950","inchi_key":"","kegg_id":"C00041","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Arginine","moverz_quant":"345.21, 170.9","ri":"2.68","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6322","inchi_key":"","kegg_id":"C00062","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Asparagine","moverz_quant":"303.13, 170.9","ri":"2.25","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6267","inchi_key":"","kegg_id":"C00152","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Aspartic Acid","moverz_quant":"304.11, 171","ri":"2.99","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5960","inchi_key":"","kegg_id":"C00049","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Citrulline","moverz_quant":"346.21, 171","ri":"3.33","ri_type":"UFLORIDA_MS_RI","pubchem_id":"9750","inchi_key":"","kegg_id":"C00327","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Glutamic Acid","moverz_quant":"318.11, 171.1","ri":"3.35","ri_type":"UFLORIDA_MS_RI","pubchem_id":"33032","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Glutamine","moverz_quant":"317.21, 171","ri":"2.65","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5961","inchi_key":"","kegg_id":"C00064","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Glycine","moverz_quant":"246.08, 170.9","ri":"2.81","ri_type":"UFLORIDA_MS_RI","pubchem_id":"750","inchi_key":"","kegg_id":"C00037","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Histidine","moverz_quant":"326.21, 110","ri":"2.07","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6274","inchi_key":"","kegg_id":"C00135","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Isoleucine","moverz_quant":"302.21, 170.9","ri":"8.15","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6306","inchi_key":"","kegg_id":"C00407","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Leucine","moverz_quant":"302.21, 170.9","ri":"8.26","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6106","inchi_key":"","kegg_id":"C00123","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Lysine","moverz_quant":"487.21, 170.9","ri":"6.66","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5962","inchi_key":"","kegg_id":"C00047","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Methionine","moverz_quant":"320.21, 170.9","ri":"6.87","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6137","inchi_key":"","kegg_id":"C00073","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Ornithine","moverz_quant":"473.22, 170.9","ri":"5.93","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6262","inchi_key":"","kegg_id":"C00077","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Phenylalanine","moverz_quant":"336.21, 170.9","ri":"8.40","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6140","inchi_key":"","kegg_id":"C00079","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Proline","moverz_quant":"286.16, 171","ri":"4.84","ri_type":"UFLORIDA_MS_RI","pubchem_id":"145742","inchi_key":"","kegg_id":"C00148","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Serine","moverz_quant":"276.11, 170.9","ri":"2.60","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5951","inchi_key":"","kegg_id":"C00065","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Threonine","moverz_quant":"290.11, 170.9","ri":"3.74","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6288","inchi_key":"","kegg_id":"C00188","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Tryptophan","moverz_quant":"375.21, 171","ri":"8.54","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6305","inchi_key":"","kegg_id":"C00078","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Tyrosine","moverz_quant":"352.21, 170.9","ri":"6.66","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6057","inchi_key":"","kegg_id":"C00082","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Valine","moverz_quant":"288.21, 171","ri":"7.09","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6287","inchi_key":"","kegg_id":"C00183","other_id":"","other_id_type":"UFLORIDA_FEATURE"}]
}

}