{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000251","ANALYSIS_ID":"AN000397","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Intracellular Amino Acid levels in response to MYC","PROJECT_TYPE":"Targeted LC-MS Metabolomics","PROJECT_SUMMARY":"Hypothesis:MYC is suppressing the autophagy pathway which leads to impaired of proteins and reduced free Amino Acids in the cells.","INSTITUTE":"Moffitt Cancer Center","DEPARTMENT":"Tumor Biology","LAST_NAME":"Cleveland","FIRST_NAME":"John","ADDRESS":"12902 Magnolia Dr","EMAIL":"John.Cleveland@moffitt.org","PHONE":"813 745 3888","FUNDING_SOURCE":"NIH"},

"STUDY":{"STUDY_TITLE":"Amino Acid levels in preB cells under starvation","STUDY_TYPE":"treatment","STUDY_SUMMARY":"pre B cells from wt and EuMyc mice were grown in normal media and then placed 2hrs in EBSS.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LAST_NAME":"Schaub","FIRST_NAME":"Franz","ADDRESS":"R3-226 Academic Research Building, Department of Biochemistry and Molecular PO Box 100245, Gainesville, FL 32610-0245","EMAIL":"Franz.Schaub@moffitt.org","PHONE":"-","NUM_GROUPS":"4","TOTAL_SUBJECTS":"12"},

"SUBJECT":{"SUBJECT_TYPE":"Animal cells","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","ANIMAL_ANIMAL_SUPPLIER":"inhouse breeding","CELL_BIOSOURCE_OR_SUPPLIER":"BM derived B cells","CELL_STRAIN_DETAILS":"wt and EuMyc","CELL_PRIMARY_IMMORTALIZED":"primary","SPECIES_GROUP":"Mammal"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"CC268_EBSS1",
"Factors":{"Treatment":"EuMyc transgene","media":"EBSS"}
},
{
"Subject ID":"-",
"Sample ID":"CC268_EBSS2",
"Factors":{"Treatment":"EuMyc transgene","media":"EBSS"}
},
{
"Subject ID":"-",
"Sample ID":"CC268_EBSS3",
"Factors":{"Treatment":"EuMyc transgene","media":"EBSS"}
},
{
"Subject ID":"-",
"Sample ID":"CC268_N1",
"Factors":{"Treatment":"EuMyc transgene","media":"normal media"}
},
{
"Subject ID":"-",
"Sample ID":"CC268_N2",
"Factors":{"Treatment":"EuMyc transgene","media":"normal media"}
},
{
"Subject ID":"-",
"Sample ID":"CC268_N3",
"Factors":{"Treatment":"EuMyc transgene","media":"normal media"}
},
{
"Subject ID":"-",
"Sample ID":"CC267_EBSS1",
"Factors":{"Treatment":"wt","media":"EBSS"}
},
{
"Subject ID":"-",
"Sample ID":"CC267_EBSS2",
"Factors":{"Treatment":"wt","media":"EBSS"}
},
{
"Subject ID":"-",
"Sample ID":"CC267_EBSS3",
"Factors":{"Treatment":"wt","media":"EBSS"}
},
{
"Subject ID":"-",
"Sample ID":"CC267_N1",
"Factors":{"Treatment":"wt","media":"normal media"}
},
{
"Subject ID":"-",
"Sample ID":"CC267_N2",
"Factors":{"Treatment":"wt","media":"normal media"}
},
{
"Subject ID":"-",
"Sample ID":"CC267_N3",
"Factors":{"Treatment":"wt","media":"normal media"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cells were counted, washed with cold PBS and then flash-frozen in liquid N2","SAMPLE_TYPE":"cells","VOLUMEORAMOUNT_COLLECTED":"0.5.00E+07 cells","STORAGE_VIALS":"screw caps"},

"TREATMENT":{"TREATMENT_SUMMARY":"Cells were incubated for 2hrs in EBSS at 37C.","CELL_MEDIA":"45%DMEM, 45%IMDM, 10%FCS, Q, PS, Sodium Pyruvate, beta mercaptoethanol and EBSS 10% dialized FBS","CELL_HARVESTING":"washed, and pellet flashfroozen in liquid N2","CELL_PCT_CONFLUENCE":"2xE06 per ml"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Amino acid","SAMPLEPREP_PROTOCOL_FILENAME":"SB_AA_Assay.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Amino Acid","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 6490 QQQ","COLUMN_NAME":"Waters AccQ Tag (100 x 2mm, 1.7um)","METHODS_FILENAME":"SB_AA_Assay.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Sanford Burnham","DETECTOR_TYPE":"Triple Quadrupole"},

"MS":{"INSTRUMENT_NAME":"Agilent 6490 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"Amino Acids"},

"MS_METABOLITE_DATA":{
"Units":"pmol/million cells",

"Data":[{"Metabolite":"Alanine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"286.1697","CC268_N2":"325.3258","CC268_N3":"274.6649","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"150.3779","CC267_N2":"167.2620","CC267_N3":""},{"Metabolite":"Arginine","CC268_EBSS1":"6.4687","CC268_EBSS2":"7.0423","CC268_EBSS3":"7.3493","CC268_N1":"53.6907","CC268_N2":"53.4811","CC268_N3":"47.9826","CC267_EBSS1":"6.9324","CC267_EBSS2":"5.5625","CC267_EBSS3":"6.1154","CC267_N1":"","CC267_N2":"39.5995","CC267_N3":"37.8744"},{"Metabolite":"Asparagine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"55.3738","CC268_N2":"59.4364","CC268_N3":"56.9143","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"38.5385","CC267_N2":"46.7229","CC267_N3":"41.0002"},{"Metabolite":"Aspartic Acid","CC268_EBSS1":"144.5106","CC268_EBSS2":"149.8918","CC268_EBSS3":"134.1733","CC268_N1":"548.4718","CC268_N2":"505.6645","CC268_N3":"520.8316","CC267_EBSS1":"141.6219","CC267_EBSS2":"128.0513","CC267_EBSS3":"128.5390","CC267_N1":"358.8802","CC267_N2":"449.5085","CC267_N3":"431.9556"},{"Metabolite":"Citrulline","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"","CC268_N2":"","CC268_N3":"","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"","CC267_N2":"","CC267_N3":""},{"Metabolite":"Glutamic Acid","CC268_EBSS1":"505.8097","CC268_EBSS2":"452.5393","CC268_EBSS3":"522.7460","CC268_N1":"1618.2563","CC268_N2":"1831.7335","CC268_N3":"1844.1606","CC267_EBSS1":"365.9140","CC267_EBSS2":"348.5579","CC267_EBSS3":"377.9552","CC267_N1":"1113.5555","CC267_N2":"1185.7408","CC267_N3":"1178.8394"},{"Metabolite":"Glutamine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"2696.7081","CC268_N2":"2837.0207","CC268_N3":"2669.1879","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"2145.4604","CC267_N2":"2579.7559","CC267_N3":"2459.9461"},{"Metabolite":"Glycine","CC268_EBSS1":"289.8055","CC268_EBSS2":"330.7167","CC268_EBSS3":"324.9333","CC268_N1":"880.4140","CC268_N2":"1006.6005","CC268_N3":"897.9364","CC267_EBSS1":"173.7769","CC267_EBSS2":"181.0660","CC267_EBSS3":"152.0332","CC267_N1":"458.6096","CC267_N2":"522.0940","CC267_N3":"489.4995"},{"Metabolite":"Histidine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"30.6960","CC268_N2":"33.7524","CC268_N3":"29.6564","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"24.8340","CC267_N2":"28.7767","CC267_N3":"22.4751"},{"Metabolite":"Isoleucine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"95.8893","CC268_N2":"129.8472","CC268_N3":"95.3572","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"72.2414","CC267_N2":"87.9081","CC267_N3":"77.9505"},{"Metabolite":"Leucine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"94.8523","CC268_N2":"109.4593","CC268_N3":"109.8084","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"65.0017","CC267_N2":"81.7588","CC267_N3":"72.7164"},{"Metabolite":"Lysine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"","CC268_N2":"","CC268_N3":"","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"","CC267_N2":"","CC267_N3":""},{"Metabolite":"Methionine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"96.2030","CC268_N2":"101.7481","CC268_N3":"95.5282","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"88.1543","CC267_N2":"92.3585","CC267_N3":"89.1620"},{"Metabolite":"Ornithine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"","CC268_N2":"","CC268_N3":"","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"","CC267_N2":"","CC267_N3":""},{"Metabolite":"Phenylalanine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"55.9604","CC268_N2":"75.1085","CC268_N3":"51.9415","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"37.3831","CC267_N2":"39.7466","CC267_N3":"40.4254"},{"Metabolite":"Proline","CC268_EBSS1":"82.7617","CC268_EBSS2":"91.8553","CC268_EBSS3":"90.8409","CC268_N1":"359.3972","CC268_N2":"338.1663","CC268_N3":"344.0594","CC267_EBSS1":"67.9805","CC267_EBSS2":"57.6340","CC267_EBSS3":"57.9749","CC267_N1":"118.8846","CC267_N2":"144.4926","CC267_N3":"117.2358"},{"Metabolite":"Serine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"220.4652","CC268_N2":"251.8763","CC268_N3":"230.9355","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"202.7734","CC267_N2":"203.0658","CC267_N3":"205.8240"},{"Metabolite":"Threonine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"388.4981","CC268_N2":"434.5581","CC268_N3":"405.0570","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"302.6392","CC267_N2":"350.2393","CC267_N3":"287.6696"},{"Metabolite":"Tryptophan","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"12.4508","CC268_N2":"15.2310","CC268_N3":"11.2522","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"10.7284","CC267_N2":"10.8762","CC267_N3":"10.0154"},{"Metabolite":"Tyrosine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"65.5446","CC268_N2":"71.2255","CC268_N3":"60.9647","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"56.0753","CC267_N2":"59.8148","CC267_N3":"54.4807"},{"Metabolite":"Valine","CC268_EBSS1":"","CC268_EBSS2":"","CC268_EBSS3":"","CC268_N1":"93.9793","CC268_N2":"110.2788","CC268_N3":"96.4608","CC267_EBSS1":"","CC267_EBSS2":"","CC267_EBSS3":"","CC267_N1":"70.2507","CC267_N2":"86.6458","CC267_N3":"75.4704"}],

"Metabolites":[{"Metabolite":"Alanine","moverz_quant":"260.17, 171","ri":"4.16","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5950","inchi_key":"","kegg_id":"C00041","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Arginine","moverz_quant":"345.21, 170.9","ri":"2.68","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6322","inchi_key":"","kegg_id":"C00062","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Asparagine","moverz_quant":"303.13, 170.9","ri":"2.25","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6267","inchi_key":"","kegg_id":"C00152","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Aspartic Acid","moverz_quant":"304.11, 171","ri":"2.99","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5960","inchi_key":"","kegg_id":"C00049","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Citrulline","moverz_quant":"346.21, 171","ri":"3.33","ri_type":"UFLORIDA_MS_RI","pubchem_id":"9750","inchi_key":"","kegg_id":"C00327","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Glutamic Acid","moverz_quant":"318.11, 171.1","ri":"3.35","ri_type":"UFLORIDA_MS_RI","pubchem_id":"33032","inchi_key":"","kegg_id":"","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Glutamine","moverz_quant":"317.21, 171","ri":"2.65","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5961","inchi_key":"","kegg_id":"C00064","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Glycine","moverz_quant":"246.08, 170.9","ri":"2.81","ri_type":"UFLORIDA_MS_RI","pubchem_id":"750","inchi_key":"","kegg_id":"C00037","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Histidine","moverz_quant":"326.21, 110","ri":"2.07","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6274","inchi_key":"","kegg_id":"C00135","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Isoleucine","moverz_quant":"302.21, 170.9","ri":"8.15","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6306","inchi_key":"","kegg_id":"C00407","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Leucine","moverz_quant":"302.21, 170.9","ri":"8.26","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6106","inchi_key":"","kegg_id":"C00123","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Lysine","moverz_quant":"487.21, 170.9","ri":"6.66","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5962","inchi_key":"","kegg_id":"C00047","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Methionine","moverz_quant":"320.21, 170.9","ri":"6.87","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6137","inchi_key":"","kegg_id":"C00073","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Ornithine","moverz_quant":"473.22, 170.9","ri":"5.93","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6262","inchi_key":"","kegg_id":"C00077","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Phenylalanine","moverz_quant":"336.21, 170.9","ri":"8.40","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6140","inchi_key":"","kegg_id":"C00079","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Proline","moverz_quant":"286.16, 171","ri":"4.84","ri_type":"UFLORIDA_MS_RI","pubchem_id":"145742","inchi_key":"","kegg_id":"C00148","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Serine","moverz_quant":"276.11, 170.9","ri":"2.60","ri_type":"UFLORIDA_MS_RI","pubchem_id":"5951","inchi_key":"","kegg_id":"C00065","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Threonine","moverz_quant":"290.11, 170.9","ri":"3.74","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6288","inchi_key":"","kegg_id":"C00188","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Tryptophan","moverz_quant":"375.21, 171","ri":"8.54","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6305","inchi_key":"","kegg_id":"C00078","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Tyrosine","moverz_quant":"352.21, 170.9","ri":"6.66","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6057","inchi_key":"","kegg_id":"C00082","other_id":"","other_id_type":"UFLORIDA_FEATURE"},{"Metabolite":"Valine","moverz_quant":"288.21, 171","ri":"7.09","ri_type":"UFLORIDA_MS_RI","pubchem_id":"6287","inchi_key":"","kegg_id":"C00183","other_id":"","other_id_type":"UFLORIDA_FEATURE"}]
}

}