{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000255","ANALYSIS_ID":"AN000403","VERSION":"1","CREATED_ON":"2016-09-17"},

"PROJECT":{"PROJECT_TITLE":"Metabolomics of Neonatal Pulmonary Hypertension (in Preterm Infants)","PROJECT_TYPE":"Case-control study","PROJECT_SUMMARY":"This study aimed to determine metabolic patterns in cord blood associated with development of pulmonary hypertension (PH) in human preterm infants. Umbilical blood was acquired from a nested case-control study of 20 premature age <37 weeks) infants with PH (cases) and 20 infants without PH (controls) from Prentice NICU birth cohort. Case and control infants were matched by gender, and gestational age. Archived cord blood plasma underwent metabolomics","INSTITUTE":"University of California, Davis","DEPARTMENT":"Department of Pediatrics, School of Medicine","LABORATORY":"Wedgwood lab","LAST_NAME":"Wedgwood","FIRST_NAME":"Stephen","ADDRESS":"1002B, Research II, 4625 2nd Ave., Sacramento, CA 95817","EMAIL":"stephen.wedwood@ucdmc.ucdavis.edu","PHONE":"+1-916-734-1518","FUNDING_SOURCE":"National Institute of Health U24 DK092993"},

"STUDY":{"STUDY_TITLE":"NIH WCMC Pilot & Feasibility Project: “Metabolomics of Neonatal Pulmonary in human","STUDY_TYPE":"Case-control study","STUDY_SUMMARY":"Targeted metabolomic analyses of oxylipins were performed on 40 human umbilical plasma samples collected from infants born prematurely (gestational age <37 Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring after negative mode electrospray ionization.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Nutrition","LABORATORY":"Newman Lab","LAST_NAME":"Newman","FIRST_NAME":"John","ADDRESS":"430 W. Health Sciences Dr., Davis, CA 95616","EMAIL":"john.newman@ars.usda.gov","PHONE":"+1-530-752-1009","NUM_GROUPS":"4","TOTAL_SUBJECTS":"16"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"Gestational age <37 weeks"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"BP009",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP028",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP090",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP122",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP169",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP191",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP253",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP332",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP333",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP342",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP369",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP388",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP406",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP408",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP476",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP496",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP588",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP700",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP751",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP819",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP875",
"Factors":{"Treatment":"no PH (no pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP010",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP121",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP132",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP158",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP185",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP289",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP295",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP312",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP385",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP407",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP497",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP515",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP516",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP522",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP541",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP732",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"BP832",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"P003",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"P288",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"P295",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
},
{
"Subject ID":"-",
"Sample ID":"P302",
"Factors":{"Treatment":"PH (pulmonary hypertension)"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","COLLECTION_PROTOCOL_FILENAME":"NewmanLab-FreeOxys-Protocol-P.pdf","SAMPLE_TYPE":"Umbilical cord plasma","STORAGE_CONDITIONS":"- 80°C","STORAGE_VIALS":"Eppendorf tubes"},

"TREATMENT":{"TREATMENT_SUMMARY":"Nested case-control study","TREATMENT_PROTOCOL_FILENAME":"NewmanLab-FreeOxys-Protocol-P.pdf"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Plasma was added directly to a Waters Ostro plate, mixed with the surrogates, and acetonitrile and the extract eluted through. The extracts were dried, in 100?L 50:50 methanol:acetonitrile with internal standards and filtered at 0.1 µm prior to analysis.","SAMPLEPREP_PROTOCOL_FILENAME":"NewmanLab-FreeOxys-Protocol-P.pdf","EXTRACTION_METHOD":"Ostro plate","EXTRACT_CLEANUP":"Ostro plate","EXTRACT_STORAGE":"- 20 °C","SAMPLE_RESUSPENSION":"100 µL","SAMPLE_SPIKING":"See sample prep protocol file","ORGAN":"Umbilical cord plasma"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Oxylipin analysis","CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"-","METHODS_FILENAME":"NewmanLab-FreeOxys-Protocol-P.pdf","COLUMN_TEMPERATURE":"60 C","FLOW_GRADIENT":"See protocol/methods file","FLOW_RATE":"0.25","INTERNAL_STANDARD":"See protocol/methods file","RETENTION_TIME":"See protocol/methods file","SAMPLE_INJECTION":"5 L","SOLVENT_A":"0.1% acetic acid","SOLVENT_B":"90% ACN / 10% IPA","ANALYTICAL_TIME":"16 min","WEAK_WASH_SOLVENT_NAME":"20% methanol, 10% isopropanol","WEAK_WASH_VOLUME":"600 L","STRONG_WASH_SOLVENT_NAME":"50:50 Acetonitrile:Methanol","STRONG_WASH_VOLUME":"600 L","SAMPLE_LOOP_SIZE":"17 L"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Newman","SOFTWARE_VERSION":"AB Sciex MultiQuant version 3.0.1","OPERATOR_NAME":"Michael La Frano","DETECTOR_TYPE":"API 4000 QTrap (AB Sciex, Framingham, MA, USA)","DATA_FORMAT":".mzML"},

"MS":{"INSTRUMENT_NAME":"ABI Sciex API 4000 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","CAPILLARY_TEMPERATURE":"See protocol file","CAPILLARY_VOLTAGE":"NEGATIVE","MS_COMMENTS":"Oxylipins analysis"},

"MS_METABOLITE_DATA":{
"Units":"% Area Ratio",

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