{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000290","ANALYSIS_ID":"AN000462","VERSION":"1","CREATED_ON":"December 27, 2015, 7:49 am"},

"PROJECT":{"PROJECT_TITLE":"Climate Change and Starch Yield in Maize","PROJECT_TYPE":"MS study","PROJECT_SUMMARY":"Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.","INSTITUTE":"University of Florida","DEPARTMENT":"Chemistry","LABORATORY":"Stewart","LAST_NAME":"Stewart","FIRST_NAME":"Jon","ADDRESS":"126 Sisler Hall","EMAIL":"jds2@chem.ufl.edu","PHONE":"846-0743","FUNDING_SOURCE":"USDA"},

"STUDY":{"STUDY_TITLE":"2014 Biotron Experiment Metabolites","STUDY_TYPE":"Time course","STUDY_SUMMARY":"Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LABORATORY":"Stewart","LAST_NAME":"Stewart","FIRST_NAME":"Jon D.","ADDRESS":"102 Leigh Hall","EMAIL":"jds2@chem.ufl.edu","PHONE":"352-846-0743","NUM_GROUPS":"3","TOTAL_SUBJECTS":"47","STUDY_COMMENTS":"Normal day / normal night (17 samples); Hot day / normal night (14 samples); Hot day / hot night (17 samples)"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Zea mays"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"N_N_plant4_14DAP",
"Factors":{"Temperature":"Control","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant10_14DAP",
"Factors":{"Temperature":"Control","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant20_14DAP",
"Factors":{"Temperature":"Control","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant1_18DAP",
"Factors":{"Temperature":"Control","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant5_18DAP",
"Factors":{"Temperature":"Control","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant17_18DAP",
"Factors":{"Temperature":"Control","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant14_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant21_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant22_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant24_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant6_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant8_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant15_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant16_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant3_30DAP",
"Factors":{"Temperature":"Control","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant7_30DAP",
"Factors":{"Temperature":"Control","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant12_30DAP",
"Factors":{"Temperature":"Control","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant1_14DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant2_14DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant22_14DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant6_18DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant20_18DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant23_18DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant13_22DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant21_22DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant4_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant5_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant9_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant17_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant3_30DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant8_30DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant7_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant8_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant16_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant18_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant10_18DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant17_18DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant22_18DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant2_22DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant15_22DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant24_22DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant4_26DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant14_26DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant16_26DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant3_30DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant21_30DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant23_30DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"30"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"n/a","SAMPLE_TYPE":"Maize kernel","COLLECTION_METHOD":"manual removal from maize ear","COLLECTION_LOCATION":"University of Wisconsin Biotron","STORAGE_CONDITIONS":"Frozen at -80C"},

"TREATMENT":{"TREATMENT_SUMMARY":"n/a","PLANT_GROWTH_LOCATION":"Wisconsin Biotron Facility","PLANT_PLOT_DESIGN":"Random placement in growth facility","PLANT_LIGHT_PERIOD":"16 h per day","PLANT_TEMP":"Normal day / normal night (28C day / 17C night; Hot day / normal night (38C / 17C); Hot day / hot night (38C / 28C)","PLANT_WATERING_REGIME":"Fully watered","PLANT_GROWTH_STAGE":"Varied","PLANT_METAB_QUENCH_METHOD":"Frozen at -80C"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"n/a","SAMPLEPREP_PROTOCOL_FILENAME":"Maize_Tissue_sample_prep.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Scientific-Dionex Ultimate 3000","COLUMN_NAME":"ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)","METHODS_FILENAME":"Instrument Parameters.pdf","INTERNAL_STANDARD":"Appendix A - Internal Standard Prep GLCMS.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"Instrument Parameters.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap Thermo Scientific- Q Exactive"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"(R)-MALATE","N_N_plant3_30DAP":"5.82E+07","N_N_plant12_30DAP":"3.78E+07","N_N_plant16_26DAP":"5.72E+07","N_N_plant8_26DAP":"5.56E+07","N_N_plant15_26DAP":"5.54E+07","N_N_plant24_22DAP":"8.22E+07","N_N_plant7_30DAP":"4.36E+07","N_N_plant6_26DAP":"4.86E+07","H_N_plant21_22DAP":"4.87E+07","H_N_plant5_26DAP":"4.2E+07","H_N_plant4_26DAP":"4.56E+07","H_N_plant20_18DAP":"1.04E+08","H_N_plant17_26DAP":"5.14E+07","H_N_plant23_18DAP":"9.58E+07","H_N_plant1_14DAP":"1.5E+08","H_N_plant22_14DAP":"1.35E+08","H_N_plant3_30DAP":"4.27E+07","H_N_plant13_22DAP":"6.89E+07","H_N_plant9_26DAP":"6.06E+07","H_N_plant6_18DAP":"1.13E+08","H_H_plant2_22DAP":"5.02E+07","N_N_plant4_14DAP":"1.65E+08","H_N_plant8_30DAP":"3.27E+07","H_H_plant22_18DAP":"7.34E+07","H_H_plant17_18DAP":"1.05E+08","H_H_plant10_18DAP":"9.35E+07","H_H_plant16_14DAP":"1.53E+08","H_N_plant2_14DAP":"1.5E+08","H_H_plant7_14DAP":"1.53E+08","N_N_plant10_14DAP":"1.51E+08","H_H_plant4_26DAP":"3.99E+07","H_H_plant8_14DAP":"1.39E+08","H_H_plant23_30DAP":"2.21E+07","H_H_plant21_30DAP":"2.28E+07","H_H_plant3_30DAP":"1.98E+07","H_H_plant15_22DAP":"4.35E+07","H_H_plant16_26DAP":"3.64E+07","H_H_plant24_22DAP":"4.75E+07","H_H_plant18_14DAP":"1.42E+08","N_N_plant20_14DAP":"1.46E+08","H_H_plant14_26DAP":"3.3E+07","N_N_plant1_18DAP":"1.33E+08","N_N_plant14_22DAP":"7.42E+07","N_N_plant22_22DAP":"7.45E+07","N_N_plant5_18DAP":"1.2E+08","N_N_plant21_22DAP":"7.66E+07","N_N_plant17_18DAP":"1.18E+08"},{"Metabolite":"1-AMINOCYCLOPROPANE-1-CARBOXYLATE","N_N_plant3_30DAP":"6.67E+07","N_N_plant12_30DAP":"5.39E+07","N_N_plant16_26DAP":"7.82E+07","N_N_plant8_26DAP":"7.06E+07","N_N_plant15_26DAP":"6.3E+07","N_N_plant24_22DAP":"7.99E+07","N_N_plant7_30DAP":"5.46E+07","N_N_plant6_26DAP":"7.16E+07","H_N_plant21_22DAP":"6.73E+07","H_N_plant5_26DAP":"5.93E+07","H_N_plant4_26DAP":"6.42E+07","H_N_plant20_18DAP":"1.21E+08","H_N_plant17_26DAP":"9.36E+07","H_N_plant23_18DAP":"8.97E+07","H_N_plant1_14DAP":"1.48E+08","H_N_plant22_14DAP":"1.24E+08","H_N_plant3_30DAP":"6.62E+07","H_N_plant13_22DAP":"9.63E+07","H_N_plant9_26DAP":"8.19E+07","H_N_plant6_18DAP":"9.95E+07","H_H_plant2_22DAP":"1E+08","N_N_plant4_14DAP":"1.36E+08","H_N_plant8_30DAP":"5.58E+07","H_H_plant22_18DAP":"1.22E+08","H_H_plant17_18DAP":"1.2E+08","H_H_plant10_18DAP":"1.51E+08","H_H_plant16_14DAP":"1.52E+08","H_N_plant2_14DAP":"1.27E+08","H_H_plant7_14DAP":"1.66E+08","N_N_plant10_14DAP":"1.48E+08","H_H_plant4_26DAP":"7.07E+07","H_H_plant8_14DAP":"1.47E+08","H_H_plant23_30DAP":"2.6E+07","H_H_plant21_30DAP":"3.65E+07","H_H_plant3_30DAP":"3.2E+07","H_H_plant15_22DAP":"1.13E+08","H_H_plant16_26DAP":"6.37E+07","H_H_plant24_22DAP":"8.88E+07","H_H_plant18_14DAP":"1.54E+08","N_N_plant20_14DAP":"1.82E+08","H_H_plant14_26DAP":"6.39E+07","N_N_plant1_18DAP":"1.2E+08","N_N_plant14_22DAP":"7.73E+07","N_N_plant22_22DAP":"8.67E+07","N_N_plant5_18DAP":"1.14E+08","N_N_plant21_22DAP":"9.27E+07","N_N_plant17_18DAP":"9.64E+07"},{"Metabolite":"3'-CMP","N_N_plant3_30DAP":"1.16E+05","N_N_plant12_30DAP":"3.21E+05","N_N_plant16_26DAP":"7.29E+05","N_N_plant8_26DAP":"5.76E+05","N_N_plant15_26DAP":"6.35E+05","N_N_plant24_22DAP":"6.11E+03","N_N_plant7_30DAP":"5.22E+05","N_N_plant6_26DAP":"7.49E+05","H_N_plant21_22DAP":"6.86E+05","H_N_plant5_26DAP":"9.56E+04","H_N_plant4_26DAP":"6.16E+05","H_N_plant20_18DAP":"997","H_N_plant17_26DAP":"6.15E+05","H_N_plant23_18DAP":"3.4E+03","H_N_plant1_14DAP":"1.04E+03","H_N_plant22_14DAP":"1.2E+03","H_N_plant3_30DAP":"1.12E+05","H_N_plant13_22DAP":"2.75E+05","H_N_plant9_26DAP":"1.47E+05","H_N_plant6_18DAP":"1.95E+03","H_H_plant2_22DAP":"1.12E+06","N_N_plant4_14DAP":"774","H_N_plant8_30DAP":"6.37E+05","H_H_plant22_18DAP":"2.3E+04","H_H_plant17_18DAP":"3.02E+03","H_H_plant10_18DAP":"2.47E+04","H_H_plant16_14DAP":"1.26E+03","H_N_plant2_14DAP":"1.25E+03","H_H_plant7_14DAP":"1.65E+03","N_N_plant10_14DAP":"1.28E+03","H_H_plant4_26DAP":"5.8E+05","H_H_plant8_14DAP":"683","H_H_plant23_30DAP":"9.75E+04","H_H_plant21_30DAP":"1.71E+05","H_H_plant3_30DAP":"2.22E+05","H_H_plant15_22DAP":"8.66E+05","H_H_plant16_26DAP":"2.7E+05","H_H_plant24_22DAP":"7.35E+05","H_H_plant18_14DAP":"1E+03","N_N_plant20_14DAP":"1.55E+03","H_H_plant14_26DAP":"6.2E+04","N_N_plant1_18DAP":"1.12E+03","N_N_plant14_22DAP":"4.41E+05","N_N_plant22_22DAP":"1.45E+04","N_N_plant5_18DAP":"0","N_N_plant21_22DAP":"1.03E+05","N_N_plant17_18DAP":"946"},{"Metabolite":"4-AMINOBUTANOATE","N_N_plant3_30DAP":"1.88E+08","N_N_plant12_30DAP":"1.34E+08","N_N_plant16_26DAP":"1.26E+08","N_N_plant8_26DAP":"1.62E+08","N_N_plant15_26DAP":"1.24E+08","N_N_plant24_22DAP":"1.21E+08","N_N_plant7_30DAP":"1.43E+08","N_N_plant6_26DAP":"1.38E+08","H_N_plant21_22DAP":"1.66E+08","H_N_plant5_26DAP":"1.74E+08","H_N_plant4_26DAP":"1.93E+08","H_N_plant20_18DAP":"2.29E+08","H_N_plant17_26DAP":"2.68E+08","H_N_plant23_18DAP":"1.43E+08","H_N_plant1_14DAP":"2.27E+08","H_N_plant22_14DAP":"1.38E+08","H_N_plant3_30DAP":"2.56E+08","H_N_plant13_22DAP":"2.58E+08","H_N_plant9_26DAP":"2.38E+08","H_N_plant6_18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}

}