{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000290","ANALYSIS_ID":"AN000463","VERSION":"1","CREATED_ON":"December 27, 2015, 7:49 am"},

"PROJECT":{"PROJECT_TITLE":"Climate Change and Starch Yield in Maize","PROJECT_TYPE":"MS study","PROJECT_SUMMARY":"Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.","INSTITUTE":"University of Florida","DEPARTMENT":"Chemistry","LABORATORY":"Stewart","LAST_NAME":"Stewart","FIRST_NAME":"Jon","ADDRESS":"126 Sisler Hall","EMAIL":"jds2@chem.ufl.edu","PHONE":"846-0743","FUNDING_SOURCE":"USDA"},

"STUDY":{"STUDY_TITLE":"2014 Biotron Experiment Metabolites","STUDY_TYPE":"Time course","STUDY_SUMMARY":"Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.","INSTITUTE":"University of Florida","DEPARTMENT":"SECIM","LABORATORY":"Stewart","LAST_NAME":"Stewart","FIRST_NAME":"Jon D.","ADDRESS":"102 Leigh Hall","EMAIL":"jds2@chem.ufl.edu","PHONE":"352-846-0743","NUM_GROUPS":"3","TOTAL_SUBJECTS":"47","STUDY_COMMENTS":"Normal day / normal night (17 samples); Hot day / normal night (14 samples); Hot day / hot night (17 samples)"},

"SUBJECT":{"SUBJECT_TYPE":"Plant","SUBJECT_SPECIES":"Zea mays"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"N_N_plant4_14DAP",
"Factors":{"Temperature":"Control","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant10_14DAP",
"Factors":{"Temperature":"Control","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant20_14DAP",
"Factors":{"Temperature":"Control","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant1_18DAP",
"Factors":{"Temperature":"Control","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant5_18DAP",
"Factors":{"Temperature":"Control","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant17_18DAP",
"Factors":{"Temperature":"Control","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant14_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant21_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant22_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant24_22DAP",
"Factors":{"Temperature":"Control","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant6_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant8_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant15_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant16_26DAP",
"Factors":{"Temperature":"Control","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant3_30DAP",
"Factors":{"Temperature":"Control","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant7_30DAP",
"Factors":{"Temperature":"Control","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"N_N_plant12_30DAP",
"Factors":{"Temperature":"Control","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant1_14DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant2_14DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant22_14DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant6_18DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant20_18DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant23_18DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant13_22DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant21_22DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant4_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant5_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant9_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant17_26DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant3_30DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_N_plant8_30DAP",
"Factors":{"Temperature":"Hot day","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant7_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant8_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant16_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant18_14DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"14"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant10_18DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant17_18DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant22_18DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"18"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant2_22DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant15_22DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant24_22DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"22"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant4_26DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant14_26DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant16_26DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"26"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant3_30DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant21_30DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"30"}
},
{
"Subject ID":"-",
"Sample ID":"H_H_plant23_30DAP",
"Factors":{"Temperature":"Both hot day and hot night","Days after pollination":"30"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"n/a","SAMPLE_TYPE":"Maize kernel","COLLECTION_METHOD":"manual removal from maize ear","COLLECTION_LOCATION":"University of Wisconsin Biotron","STORAGE_CONDITIONS":"Frozen at -80C"},

"TREATMENT":{"TREATMENT_SUMMARY":"n/a","PLANT_GROWTH_LOCATION":"Wisconsin Biotron Facility","PLANT_PLOT_DESIGN":"Random placement in growth facility","PLANT_LIGHT_PERIOD":"16 h per day","PLANT_TEMP":"Normal day / normal night (28C day / 17C night; Hot day / normal night (38C / 17C); Hot day / hot night (38C / 28C)","PLANT_WATERING_REGIME":"Fully watered","PLANT_GROWTH_STAGE":"Varied","PLANT_METAB_QUENCH_METHOD":"Frozen at -80C"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"n/a","SAMPLEPREP_PROTOCOL_FILENAME":"Maize_Tissue_sample_prep.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Scientific-Dionex Ultimate 3000","COLUMN_NAME":"ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)","METHODS_FILENAME":"Instrument Parameters.pdf","INTERNAL_STANDARD":"Appendix A - Internal Standard Prep GLCMS.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"Instrument Parameters.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap Thermo Scientific- Q Exactive"},

"MS_METABOLITE_DATA":{
"Units":"Peak area",

"Data":[{"Metabolite":"2,3-DIHYDROXYBENZOATE_1","N_N_plant6_26DAP":"6.06E+05","N_N_plant8_26DAP":"7.25E+05","N_N_plant15_26DAP":"8.43E+05","N_N_plant16_26DAP":"9.69E+05","N_N_plant7_30DAP":"6.73E+05","N_N_plant24_22DAP":"6.5E+05","N_N_plant12_30DAP":"5.44E+05","N_N_plant3_30DAP":"6.33E+05","H_N_plant23_18DAP":"4.51E+05","H_N_plant6_18DAP":"2.8E+05","H_N_plant13_22DAP":"7.11E+05","H_N_plant1_14DAP":"3.39E+05","H_N_plant22_14DAP":"2.4E+05","H_N_plant20_18DAP":"5.02E+05","N_N_plant4_14DAP":"3.26E+05","H_N_plant2_14DAP":"4.3E+05","H_N_plant21_22DAP":"6.94E+05","H_N_plant8_30DAP":"9.14E+05","H_N_plant5_26DAP":"1.02E+06","H_N_plant4_26DAP":"1.12E+06","H_N_plant3_30DAP":"5.54E+05","H_N_plant9_26DAP":"1.37E+06","H_N_plant17_26DAP":"1.13E+06","H_H_plant22_18DAP":"5.58E+05","H_H_plant15_22DAP":"9.13E+05","H_H_plant17_18DAP":"3.48E+05","H_H_plant10_18DAP":"5.2E+05","H_H_plant18_14DAP":"3.91E+05","N_N_plant10_14DAP":"3.12E+05","H_H_plant16_14DAP":"2.79E+05","H_H_plant8_14DAP":"2.57E+05","H_H_plant7_14DAP":"5.51E+05","H_H_plant4_26DAP":"9.99E+05","H_H_plant14_26DAP":"1.42E+06","H_H_plant24_22DAP":"1.06E+06","H_H_plant16_26DAP":"1.16E+06","N_N_plant20_14DAP":"4.22E+05","H_H_plant2_22DAP":"1.24E+06","H_H_plant3_30DAP":"1.31E+06","H_H_plant21_30DAP":"1.57E+06","H_H_plant23_30DAP":"6.97E+05","N_N_plant1_18DAP":"4.1E+05","N_N_plant22_22DAP":"8.89E+05","N_N_plant14_22DAP":"1.07E+06","N_N_plant17_18DAP":"3.31E+05","N_N_plant5_18DAP":"2.6E+05","N_N_plant21_22DAP":"9.51E+05"},{"Metabolite":"2,3-DIHYDROXYBENZOATE_2","N_N_plant6_26DAP":"8.58E+04","N_N_plant8_26DAP":"3.31E+04","N_N_plant15_26DAP":"9.6E+04","N_N_plant16_26DAP":"1.15E+05","N_N_plant7_30DAP":"8.52E+04","N_N_plant24_22DAP":"1.11E+05","N_N_plant12_30DAP":"5.66E+04","N_N_plant3_30DAP":"1.15E+05","H_N_plant23_18DAP":"1.64E+05","H_N_plant6_18DAP":"2.18E+05","H_N_plant13_22DAP":"2E+05","H_N_plant1_14DAP":"2.36E+05","H_N_plant22_14DAP":"2.54E+05","H_N_plant20_18DAP":"2.48E+05","N_N_plant4_14DAP":"1.76E+05","H_N_plant2_14DAP":"2.34E+05","H_N_plant21_22DAP":"9.82E+04","H_N_plant8_30DAP":"1.43E+05","H_N_plant5_26DAP":"2.09E+05","H_N_plant4_26DAP":"1.04E+05","H_N_plant3_30DAP":"6.18E+04","H_N_plant9_26DAP":"1.86E+05","H_N_plant17_26DAP":"1.51E+05","H_H_plant22_18DAP":"3.08E+05","H_H_plant15_22DAP":"3.82E+05","H_H_plant17_18DAP":"4.2E+05","H_H_plant10_18DAP":"4.51E+05","H_H_plant18_14DAP":"2.89E+05","N_N_plant10_14DAP":"2.28E+05","H_H_plant16_14DAP":"3.28E+05","H_H_plant8_14DAP":"3.25E+05","H_H_plant7_14DAP":"2.46E+05","H_H_plant4_26DAP":"4.62E+05","H_H_plant14_26DAP":"3.57E+05","H_H_plant24_22DAP":"4.52E+05","H_H_plant16_26DAP":"4.08E+05","N_N_plant20_14DAP":"1.39E+05","H_H_plant2_22DAP":"6.98E+05","H_H_plant3_30DAP":"3.49E+05","H_H_plant21_30DAP":"3.6E+05","H_H_plant23_30DAP":"1.46E+05","N_N_plant1_18DAP":"2.44E+05","N_N_plant22_22DAP":"1.44E+05","N_N_plant14_22DAP":"1.22E+05","N_N_plant17_18DAP":"1.82E+05","N_N_plant5_18DAP":"1.58E+05","N_N_plant21_22DAP":"1.65E+05"},{"Metabolite":"3-(2-HYDROXYPHENYL)PROPANOATE","N_N_plant6_26DAP":"6.16E+04","N_N_plant8_26DAP":"7.89E+04","N_N_plant15_26DAP":"5.28E+04","N_N_plant16_26DAP":"1.27E+05","N_N_plant7_30DAP":"5.44E+04","N_N_plant24_22DAP":"2.48E+05","N_N_plant12_30DAP":"4.55E+04","N_N_plant3_30DAP":"5.72E+04","H_N_plant23_18DAP":"6.31E+05","H_N_plant6_18DAP":"5.35E+05","H_N_plant13_22DAP":"1.8E+05","H_N_plant1_14DAP":"1.08E+06","H_N_plant22_14DAP":"1.33E+06","H_N_plant20_18DAP":"9.95E+05","N_N_plant4_14DAP":"1.6E+06","H_N_plant2_14DAP":"9.55E+05","H_N_plant21_22DAP":"8.19E+04","H_N_plant8_30DAP":"8.35E+04","H_N_plant5_26DAP":"7.48E+04","H_N_plant4_26DAP":"6.51E+04","H_N_plant3_30DAP":"8.62E+04","H_N_plant9_26DAP":"1.08E+05","H_N_plant17_26DAP":"1.04E+05","H_H_plant22_18DAP":"4.84E+05","H_H_plant15_22DAP":"1.51E+05","H_H_plant17_18DAP":"4.14E+05","H_H_plant10_18DAP":"9.36E+05","H_H_plant18_14DAP":"1.92E+06","N_N_plant10_14DAP":"1.45E+06","H_H_plant16_14DAP":"2.18E+06","H_H_plant8_14DAP":"1.67E+06","H_H_plant7_14DAP":"2.62E+06","H_H_plant4_26DAP":"1.27E+05","H_H_plant14_26DAP":"1.45E+05","H_H_plant24_22DAP":"2.07E+05","H_H_plant16_26DAP":"2.64E+05","N_N_plant20_14DAP":"1.53E+06","H_H_plant2_22DAP":"1.79E+05","H_H_plant3_30DAP":"2.1E+05","H_H_plant21_30DAP":"1.69E+05","H_H_plant23_30DAP":"1.8E+05","N_N_plant1_18DAP":"5.23E+05","N_N_plant22_22DAP":"1.87E+05","N_N_plant14_22DAP":"1.15E+05","N_N_plant17_18DAP":"5.35E+05","N_N_plant5_18DAP":"9.02E+05","N_N_plant21_22DAP":"1.95E+05"},{"Metabolite":"3-METHYL-2-OXOVALERIC 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}

}