{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000312","ANALYSIS_ID":"AN000498","VERSION":"1","CREATED_ON":"January 13, 2016, 4:17 pm"},

"PROJECT":{"PROJECT_TITLE":"IDH1 gliomas","PROJECT_SUMMARY":"Analyze IDH1R132H activity in glioma cell lines and tumnor tissue","INSTITUTE":"University of Michigan","DEPARTMENT":"Neurosurgery","LABORATORY":"Castro Lab","LAST_NAME":"Castro","FIRST_NAME":"Maria","ADDRESS":"Ann Arbor, MI","EMAIL":"mariacas@umich.edu","PHONE":"734-764-0850"},

"STUDY":{"STUDY_TITLE":"IDH1R132H activity in glioma cell lines and tumnor tissue (2HG)","STUDY_TYPE":"Regular","STUDY_SUMMARY":"We developed genetically engineered mice to generate brain tumors with especific genetic lessions. The animals were split in three groups: NRAS, P53 knockdown, IDH1-R132H and ATRX knock down (NPAID); NRAS, P53 knockdown, IDH1-R132H (NPI); NRAS, P53 knockdown, (NshP53). From these tumor we obtain and culture tumor cells growth like neurospheres and attached cells.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"4","TOTAL_SUBJECTS":"4"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"S00017646",
"Factors":{"Genetic makeup":"NPAID"}
},
{
"Subject ID":"-",
"Sample ID":"S00017647",
"Factors":{"Genetic makeup":"NPI"}
},
{
"Subject ID":"-",
"Sample ID":"S00017648",
"Factors":{"Genetic makeup":"NshP53"}
},
{
"Subject ID":"-",
"Sample ID":"S00020347",
"Factors":{"Genetic makeup":"Pooled cells"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"None provided.","SAMPLE_TYPE":"Cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"None provided."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"None provided.","SAMPLEPREP_PROTOCOL_FILENAME":"15DEC2015 2HG cells Castro.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 6410A QQQ","COLUMN_NAME":"Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)","METHODS_FILENAME":"2015-04-07-2HG.m.zip","METHODS_ID":"DQM0033"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6410A QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"µM/500µl extraction solvent",

"Data":[{"Metabolite":"D-2-hydroxyglutarate","S00017646":"10177.9815","S00017647":"3358.08205","S00017648":"2486.28377"},{"Metabolite":"L-2-hydroxyglutarate","S00017646":"182.37969","S00017647":"182.01126","S00017648":"454.50883"},{"Metabolite":"2-Phosphoglycerate/3-Phosphoglycerate","S00017646":"6.9806871","S00017647":"1.143851","S00017648":"4.5894202"},{"Metabolite":"6-phosphogluconic acid","S00017646":"37.638728","S00017647":"4.0799159","S00017648":"3.6714762"},{"Metabolite":"Acetyl-CoA","S00017646":"1.0653526","S00017647":"0.5927918","S00017648":"0.5995497"},{"Metabolite":"Adenosine diphosphate","S00017646":"33.130553","S00017647":"40.394093","S00017648":"41.9677"},{"Metabolite":"Adenosine monophosphate","S00017646":"153.44076","S00017647":"28.144997","S00017648":"44.537019"},{"Metabolite":"Adenosine triphosphate","S00017646":"11.329268","S00017647":"60.98457","S00017648":"67.54665"},{"Metabolite":"Citrate/iso-citrate","S00017646":"39.284676","S00017647":"7.8404032","S00017648":"3.760665"},{"Metabolite":"Fructose-6-Phosphate/glucose-6-phosphate","S00017646":"8.5134921","S00017647":"8.0508339","S00017648":"9.8884122"},{"Metabolite":"Flavin adenine dinucleotide","S00017646":"1.3357146","S00017647":"0.9016864","S00017648":"0.7557498"},{"Metabolite":"Fructose 1,6 Bisphosphate","S00017646":"40.714398","S00017647":"83.385701","S00017648":"182.38143"},{"Metabolite":"Malate","S00017646":"51.030168","S00017647":"16.612637","S00017648":"9.7442914"},{"Metabolite":"Nicotinamide adenine dinucleotide_1","S00017646":"0.6017222","S00017647":"0.076668","S00017648":"0.1132844"},{"Metabolite":"Nicotinamide adenine dinucleotide, reduced","S00017646":"","S00017647":"","S00017648":""},{"Metabolite":"Nicotinamide adenine dinucleotide phosphate","S00017646":"0.9081965","S00017647":"0.80392","S00017648":"0.6518834"},{"Metabolite":"Nicotinamide adenine dinucleotide phosphate, reduced","S00017646":"","S00017647":"","S00017648":""},{"Metabolite":"Phosphoenolpyruvate","S00017646":"0.8181748","S00017647":"0.5428246","S00017648":"1.0626774"},{"Metabolite":"D-ribulose-5-phosphate/D-xylulose-5-phosphate","S00017646":"41.767487","S00017647":"9.6581304","S00017648":"12.04663"},{"Metabolite":"Sedoheptulose-7P","S00017646":"128.23932","S00017647":"31.777984","S00017648":"31.560467"},{"Metabolite":"Succinate","S00017646":"50.19386","S00017647":"4.2907129","S00017648":"1.698174"},{"Metabolite":"Nicotinic acid","S00017646":"","S00017647":"","S00017648":""},{"Metabolite":"Nicotinamide adenine dinucleotide_2","S00017646":"0.4297534","S00017647":"0.1688857","S00017648":"0.2019378"},{"Metabolite":"Nicotinamide","S00017646":"5.6608956","S00017647":"20.430295","S00017648":"9.5880366"},{"Metabolite":"Nicotinic acid mononucleotide","S00017646":"0.1541794","S00017647":"0.155716","S00017648":"0.1544609"},{"Metabolite":"Nicotinamide mononucleotide","S00017646":"0.1721849","S00017647":"0.1247026","S00017648":"0.1338116"},{"Metabolite":"Quinolinic acid","S00017646":"0.1506504","S00017647":"0.1303641","S00017648":"0.1301834"}],

"Metabolites":[{"Metabolite":"D-2-hydroxyglutarate","retention index":"","quantified m/z":"","PubChem ID":"439391","KEGG ID":""},{"Metabolite":"L-2-hydroxyglutarate","retention index":"","quantified m/z":"","PubChem ID":"439939","KEGG ID":""},{"Metabolite":"2-Phosphoglycerate/3-Phosphoglycerate","retention index":"","quantified m/z":"","PubChem ID":"724","KEGG ID":""},{"Metabolite":"6-phosphogluconic acid","retention index":"","quantified m/z":"","PubChem ID":"91493","KEGG ID":""},{"Metabolite":"Acetyl-CoA","retention index":"","quantified m/z":"","PubChem ID":"444493","KEGG ID":""},{"Metabolite":"Adenosine diphosphate","retention index":"","quantified m/z":"","PubChem ID":"6022","KEGG ID":""},{"Metabolite":"Adenosine monophosphate","retention index":"","quantified m/z":"","PubChem ID":"6083","KEGG ID":""},{"Metabolite":"Adenosine triphosphate","retention index":"","quantified m/z":"","PubChem ID":"5957","KEGG ID":""},{"Metabolite":"Citrate/iso-citrate","retention index":"","quantified m/z":"","PubChem ID":"311","KEGG ID":""},{"Metabolite":"Fructose-6-Phosphate/glucose-6-phosphate","retention index":"","quantified m/z":"","PubChem ID":"5958","KEGG ID":""},{"Metabolite":"Flavin adenine dinucleotide","retention index":"","quantified m/z":"","PubChem ID":"46906035","KEGG ID":""},{"Metabolite":"Fructose 1,6 Bisphosphate","retention index":"","quantified m/z":"","PubChem ID":"21125049","KEGG ID":""},{"Metabolite":"Malate","retention index":"","quantified m/z":"","PubChem ID":"525","KEGG ID":""},{"Metabolite":"Nicotinamide adenine dinucleotide_1","retention index":"","quantified m/z":"","PubChem ID":"15938971","KEGG ID":""},{"Metabolite":"Nicotinamide adenine dinucleotide, reduced","retention index":"","quantified m/z":"","PubChem ID":"439153","KEGG ID":""},{"Metabolite":"Nicotinamide adenine dinucleotide phosphate","retention index":"","quantified m/z":"","PubChem ID":"15938972","KEGG ID":""},{"Metabolite":"Nicotinamide adenine dinucleotide phosphate, reduced","retention index":"","quantified m/z":"","PubChem ID":"22833512","KEGG ID":""},{"Metabolite":"Phosphoenolpyruvate","retention index":"","quantified m/z":"","PubChem ID":"1005","KEGG ID":""},{"Metabolite":"D-ribulose-5-phosphate/D-xylulose-5-phosphate","retention index":"","quantified m/z":"","PubChem ID":"77982","KEGG ID":""},{"Metabolite":"Sedoheptulose-7P","retention index":"","quantified m/z":"","PubChem ID":"165007","KEGG ID":""},{"Metabolite":"Succinate","retention index":"","quantified m/z":"","PubChem ID":"1110","KEGG ID":""},{"Metabolite":"Nicotinic acid","retention index":"","quantified m/z":"","PubChem ID":"938","KEGG ID":""},{"Metabolite":"Nicotinamide adenine dinucleotide_2","retention index":"","quantified m/z":"","PubChem ID":"439153","KEGG ID":""},{"Metabolite":"Nicotinamide","retention index":"","quantified m/z":"","PubChem ID":"936","KEGG ID":""},{"Metabolite":"Nicotinic acid mononucleotide","retention index":"","quantified m/z":"","PubChem ID":"941","KEGG ID":""},{"Metabolite":"Nicotinamide mononucleotide","retention index":"","quantified m/z":"","PubChem ID":"14180","KEGG ID":""},{"Metabolite":"Quinolinic acid","retention index":"","quantified m/z":"","PubChem ID":"1066","KEGG ID":""}]
}

}