{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000314","ANALYSIS_ID":"AN000500","VERSION":"1","CREATED_ON":"January 19, 2016, 8:28 am"},

"PROJECT":{"PROJECT_TITLE":"NSAID treatment alters the metabolomics profile of liver, kidney, lung, and heart in an experimental mouse model of heat stroke","PROJECT_SUMMARY":"Heat stroke (HS) is a significant medical threat to civilians and those serving in the U.S. Armed forces. The physiological and molecular mechanisms that are critical in HS morbidity and mortality, both during and after HS onset, remain yet to be elucidated. Current clinical biomarkers lack specificity and sensitivity to accurately diagnose HS severity, and there is a critical need for effective pharmacologic interventions and treatments that address this life-threatening disease. The systemic inflammatory response (SIR) is one target for such pharmacological interventions, as it is thought to mediate much of HS etiology. For this reason, anti-inflammatories have been directed at the SIR in an effort to treat and prevent HS. Due to their anti-inflammatory actions, non-steroidal anti-inflammatory drugs (NSAIDS) have been suggested as a treatment candidate for HS. Currently, NSAIDs are one of the most widely used medications across the world, with hundreds of millions of doses prescribed yearly. Of special concern, NSAID use is prolific throughout the U.S. Armed Forces. We recently examin4ed the effect of using NSAIDs to treat HS, and found that NSAIDs actually increase HS mortality and exacerbate the systemic organ damage (e.g., gut, kidneys) found in HS recovery in mice. Our finding suggest the use of NSAIDs by civilians and military populations may increase the risk of HS morbidity and mortality. The objective of this proposal is to exploit broad spectrum metabolomic analysis to identify new biomarkers of multi-organ damage that will improve HS diagnosis and treatment.","INSTITUTE":"US Army Research Institute","DEPARTMENT":"Environmental Medicine","LAST_NAME":"Audet; Leon","FIRST_NAME":"Gerald; Lisa","ADDRESS":"Building 42, Kansas Street, Natick, MA 01760","EMAIL":"gerald.n.audet.ctr@mail.mil","PHONE":"508.233.5959"},

"STUDY":{"STUDY_TITLE":"NSAID treatment alters the metabolomics profile of liver, kidney, lung, and heart in an experimental mouse model of heat stroke","STUDY_TYPE":"Metabolomics","STUDY_SUMMARY":"The objective of this study is to exploit broad spectrum metabolomic analysis to identify new biomarkers of multi-organ damage that will improve heat stroke (HS) diagnosis and treatment. The central hypothesis is that HS will lead to significant alterations in multi-organ metabolomics profiles that will serve as markers of HS severity, which will be shifted and intensified further by the acute use of NSAIDs. To test this hypothesis, we will be performing broad spectrum metabolomics to identify alternations in the metabolic signatures of key organs (heart, liver, kidney, and lung) in a highly validated rodent HS model leveraging implantable radiotelemetry. We will then compare these results with already completed histological gene/protein expression analysis to determine the best metabolic markers of HS induced organ damage. The results from this study will aid in the identification of preventative measures to reduce HS risk, as well as in developing therapeutics to treat multi-organ damage and facilitate recovery. The proposed study will provide the first metabolic assessment of HS severity and NSAID use, which will support future studies in HS patients to validate novel biomarkers that will improve clinical assessment of organ damage and recovery.","INSTITUTE":"RTI International","DEPARTMENT":"Systems and Translational Sciences","LABORATORY":"NIH Eastern Regional Comphrehensive Metabolomics Resource Core","LAST_NAME":"Sumner","FIRST_NAME":"Susan","ADDRESS":"3040 E. Cornwallis Road","EMAIL":"ssumner@rti.org","PHONE":"919-541-7479","NUM_GROUPS":"83"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57/BL-6","GENDER":"Male"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"L_10_299",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"260","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_10_300",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"280","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_10_303",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"290","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_10_304",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"225","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_11_422",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"200","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_11_424",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"235","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_062",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"160","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_064",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"220","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_065",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"330","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_067",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"260","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_069",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"220","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_070",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"440","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_071",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"270","sex":"Male"}
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{
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"Sample ID":"L_12_072",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"320","sex":"Male"}
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"Sample ID":"L_12_075",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"270","sex":"Male"}
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"Subject ID":"-",
"Sample ID":"L_12_076",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"200","sex":"Male"}
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{
"Subject ID":"-",
"Sample ID":"L_12_078",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"310","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_079",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"105","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_084",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"360","sex":"Male"}
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{
"Subject ID":"-",
"Sample ID":"L_12_218",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"390","sex":"Male"}
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{
"Subject ID":"-",
"Sample ID":"L_12_220",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
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{
"Subject ID":"-",
"Sample ID":"L_12_221",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"170","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_230",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"185","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_234",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"330","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_238",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"270","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_249",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"135","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_250",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"195","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_255",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_256",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"300","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_258",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"355","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_259",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"205","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_264",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"220","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_265",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_266",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"120","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_267",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"200","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_268",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"170","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_269",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"190","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_270",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"100","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_271",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"150","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_272",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"300","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_273",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"135","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_275",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"285","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_276",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"125","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_278",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"170","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_280",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"210","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_281",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"170","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_283",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"175","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_285",
"Factors":{"heat_group":"Control","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"210","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_287",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"150","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_289",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"220","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_290",
"Factors":{"heat_group":"Heat Stroke","time":"1d","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"275","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_291",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"160","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_292",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"190","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_293",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"95","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_295",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"215","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_296",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"150","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_298",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"115","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_300",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"210","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_305",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"130","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_307",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_309",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"130","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_310",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"150","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_311",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"120","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_316",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"150","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_317",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"220","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_318",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"180","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_319",
"Factors":{"heat_group":"Control","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_12_322",
"Factors":{"heat_group":"Heat Stroke","time":"T2","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"115","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_066",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"225","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_067",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_069",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"275","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_071",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"340","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_075",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"410","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_076",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"130","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_092",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"165","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_093",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"255","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_100",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"115","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_110",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"350","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_115",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"190","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_134",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"265","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_137",
"Factors":{"heat_group":"Heat Stroke","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"120","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_139",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"No Drug"},
"Additional sample data":{"approx_weight":"250","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_13_140",
"Factors":{"heat_group":"Control","time":"T3b","drug_group":"125 ug INDO"},
"Additional sample data":{"approx_weight":"80","sex":"Male"}
},
{
"Subject ID":"-",
"Sample ID":"L_CTRL_POOL_1",
"Factors":{"heat_group":"Control Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_CTRL_POOL_2",
"Factors":{"heat_group":"Control Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_CTRL_POOL_3",
"Factors":{"heat_group":"Control Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_HS_POOL_1",
"Factors":{"heat_group":"Heat Stroke Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_HS_POOL_2",
"Factors":{"heat_group":"Heat Stroke Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_HS_POOL_3",
"Factors":{"heat_group":"Heat Stroke Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_TOT_POOL_1",
"Factors":{"heat_group":"Total Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_TOT_POOL_2",
"Factors":{"heat_group":"Total Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
},
{
"Subject ID":"-",
"Sample ID":"L_TOT_POOL_3",
"Factors":{"heat_group":"Total Pool","time":"N/A","drug_group":"N/A"},
"Additional sample data":{"approx_weight":"-","sex":"-"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"None","SAMPLE_TYPE":"liver tissue","STORAGE_CONDITIONS":"-70C"},

"TREATMENT":{"TREATMENT_SUMMARY":"Mouse heat stroke model accurately simulates the thermoregulatory, inflammatory, and organ damage responses observed in HS patients. Mice were Surgically implanted with radiotelemetry devices for the continual recording of core temperatrue during heat expsoure and 24 hours of HS recorvery. Mice were orally trated with vehicle and no drug (bacon-flavored treat) or indomethacin (5 mg/kg contained in a bacon-flavored treat) immediately prior to expsoure to a heated chamger (Model 3950, Therma Forma, Marietta, OH; ambient temperature; = 39.5 degrees celsius). Mice remained in the heated chamber, in the absence of food and water, until a maximum of 42.4 degrees celsius was reached. At the maximum temperature mice were removed from the heat and either sacrificed or provided ad libitum food and water until sacrifice at ~3 hours (when mice displayed maximum hypothermia depth; ~30 degrees C) or 24 hours of recovery (24 hrs after the start of heat exposure) when ~1.0 C fever was displayed. Heart, liver, lung, and kidney were rapidly excised, sliced into transverse or longitudinal sections, and fixed in 10% neutral-buffered formalin for histological analysis (Carson Millonig Formulation, Fisher Scientific, Springfield, MA) or stored at -80 degrees celcius for molecular and metabolomics analysis.","TREATMENT_PROTOCOL_ID":"USARIEM Protocol A1002 - NSAIDS (INDO)","TREATMENT_COMPOUND":"Indomethacin","TREATMENT_ROUTE":"Oral","TREATMENT_DOSE":"5 mg/kg","TREATMENT_VEHICLE":"Bacon-flavored treat","ANIMAL_ENDP_TISSUE_COLL_LIST":"Heart, Liver, lungs and Kidneys"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Aliquots of liver sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -70 °C after being logged in for metabolomics analysis. Frozen tissue samples were transferred to labeled tubes containing stainless steel homogenization beads on dry ice to confirm weights. A total of 83 study samples were thawed on ice for sample preparation. Total tissue contents were extracted during homogenization with a 50:50 acetonitrile:water solution, to generate 2.0-2.5 mg/µl sample homogenates. Samples were centrifuged and an 80 mg equivalent volume (400/320 µl) of homogenized liver supernatants were transferred to new 2.0 mL tubes for the experimental samples. Analytical quality control (QC) phenotypic pool samples were generated by transferring a 50/40 µL aliquot of each supernatant from each respective phenotypic group based on HS-exposure or Controls into different 5 mL tubes. The two phenotypic QC pooled samples were vortexed to mix and a total study QC pool was generated by transferring 650 µL aliquots of each phenotypic pool sample into a new 5 mL tube for mixing. All pooled samples were aliquoted into labeled 2.0 mL tubes like the experimental samples, three for each QC group for an additional 9 samples. The samples were frozen for 2 hr at -70 °C and lyophilized to dryness overnight. Samples were reconstituted in 700 µl of D2O master mix containing 0.2 M Phosphate buffer, pH 7.4 + Chenomx ISTD with 6 mM Imidazole, 9:1 v/v. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"-","INSTRUMENT_NAME":"-","COLUMN_NAME":"-"},

"ANALYSIS":{"ANALYSIS_TYPE":"NMR","LABORATORY_NAME":"David H. Murdock Research Institute.","OPERATOR_NAME":"Kevin Knagge","SOFTWARE_VERSION":"TopSpin 3.2","DATA_FORMAT":"fid, 1r"},

"NM":{"INSTRUMENT_NAME":"Bruker","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"Other","FIELD_FREQUENCY_LOCK":"Deuterium","STANDARD_CONCENTRATION":"0.5 mM","SPECTROMETER_FREQUENCY":"700 MHz","NMR_PROBE":"5 mm ATMA Cryoprobe","NMR_SOLVENT":"D2O","NMR_TUBE_SIZE":"5mm, 4 inch","SHIMMING_METHOD":"Topshim","PULSE_SEQUENCE":"noesypr1d","WATER_SUPPRESSION":"yes","RECEIVER_GAIN":"4.5","OFFSET_FREQUENCY":"3299.5","CHEMICAL_SHIFT_REF_CPD":"DSS","TEMPERATURE":"298.1 K","NUMBER_OF_SCANS":"128","DUMMY_SCANS":"4","ACQUISITION_TIME":"3.893","SPECTRAL_WIDTH":"12.0227 ppm, 8.417 Hz","NUM_DATA_POINTS_ACQUIRED":"65536","REAL_DATA_POINTS":"65536","LINE_BROADENING":"0.5 Hz","ZERO_FILLING":"yes","APODIZATION":"Lorentzian","BASELINE_CORRECTION_METHOD":"Polynomial","CHEMICAL_SHIFT_REF_STD":"DSS-D6","BINNED_INCREMENT":"0.04","BINNED_DATA_EXCLUDED_RANGE":"4.755 - 4.855 (water); 7.20 - 7.35 (Imidazole)"},

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