{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000337","ANALYSIS_ID":"AN000546","VERSION":"1","CREATED_ON":"January 21, 2016, 11:48 am"},

"PROJECT":{"PROJECT_TITLE":"Metabolomics approach to allograft assessment in liver transplantation","PROJECT_TYPE":"Retrospective analysis of biobank samples","PROJECT_SUMMARY":"Organ shortage is the primary limiting factor in liver transplantation and has led to expanded utilization of livers from marginal donors. Assessment of liver allograft quality is currently based on clinical factors such as donor age, cause of death, liver function, routine clinical laboratory values and extent of graft steatosis (fat content). Expanded utilization necessitates a more detailed assessment of liver allograft quality to guide decisions regarding suitability for transplantation. Using a metabolomics approach, we plan to capture molecular snapshots of the physiologic state of the graft and to elucidate biomarkers of allograft quality and graft function post-transplant.","INSTITUTE":"Ochsner Multi-Organ Transplant Institute / SECIM","DEPARTMENT":"Abdominal Organ Transplantation","LABORATORY":"-","LAST_NAME":"Seal","FIRST_NAME":"John","ADDRESS":"1514 Jefferson Highway, New Orleans, LA 70121","EMAIL":"John.Seal@ochsner.org","PHONE":"504-232-4253","FUNDING_SOURCE":"SECIM Pilot and Feasibility Award"},

"STUDY":{"STUDY_TITLE":"Metabolomics Approach to Allograft Assessment in Liver Transplantation","STUDY_TYPE":"Retrospective analysis of biobanked liver tissue from organ donors","STUDY_SUMMARY":"This pilot study is designed to apply several types of metabolomic analysis for liver allograft assessment with the aim of identifying candidate biomarkers for allograft function and to develop a methodology that could be applied to larger scale studies. The three main categories of metabolomic analysis are reflected in each of the specific aims, including a targeted profiling of central carbon metabolism, open lipidomic and metabolomic profiling for hypothesis generation and MALDI-IMS for tissue-based spatial analysis. Each of these approaches offers potential benefits that could be optimized in a protocol for larger scale studies depending the results of the pilot investigations.","INSTITUTE":"Ochsner Multi-Organ Transplant Institute","DEPARTMENT":"SECIM","LABORATORY":"Abdominal Organ Transplantation","LAST_NAME":"Seal","FIRST_NAME":"John","ADDRESS":"1514 Jefferson Highway, New Orleans, LA 70121","EMAIL":"John.Seal@ochsner.org","PHONE":"504-232-4253","NUM_GROUPS":"3","TOTAL_SUBJECTS":"Targeted analysis and open profiling: Control group - standard criteria donor (N=15); Experimental group - donation-after-cardiac-death donors (N=10). MALDI-IMS - standard criteria donor (N=10)"},

"SUBJECT":{"SUBJECT_TYPE":"Human tissue (Decendent)","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENOTYPE_STRAIN":"Not applicable","AGE_OR_AGE_RANGE":"Variable","WEIGHT_OR_WEIGHT_RANGE":"Variable","HEIGHT_OR_HEIGHT_RANGE":"Variable","GENDER":"Variable","HUMAN_RACE":"Variable","HUMAN_ETHNICITY":"Variable","HUMAN_TRIAL_TYPE":"Not applicable","HUMAN_LIFESTYLE_FACTORS":"Not applicable","HUMAN_MEDICATIONS":"Not applicable","HUMAN_PRESCRIPTION_OTC":"Not applicable","HUMAN_SMOKING_STATUS":"Not applicable","HUMAN_ALCOHOL_DRUG_USE":"Not applicable","HUMAN_NUTRITION":"Not applicable","HUMAN_INCLUSION_CRITERIA":"Deceased organ donor with consent for use of tissue for research","HUMAN_EXCLUSION_CRITERIA":"None","ANIMAL_ANIMAL_SUPPLIER":"in house/in house","SUBJECT_COMMENTS":"Liver biopsy is routinely performed on livers used for solid organ transplantation. The tissue is from decedent subjects. Consent for use of tissue is obtained at the time of organ donation by the local organ procurement organization."},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"114291",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"1/20/2015","OPO":"AROR","ABO":"A","AGE (years)":"46","RACE":"CAU","SEX":"F","DONOR HEIGHT (cm)":"163","DONOR WEIGHT (kg)":"74.4","Hep C Positive Donor":"NO","Cold Ischemia (min)":"327","Cold Ischemia (Hrs)":"5.45","DCD DONOR":"YES","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"116707",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"2/7/2015","OPO":"LAOP","ABO":"A","AGE (years)":"52","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"175.26","DONOR WEIGHT (kg)":"96.79","Hep C Positive Donor":"NO","Cold Ischemia (min)":"278","Cold Ischemia (Hrs)":"4.63","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"115165",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"4/1/2015","OPO":"TXSB","ABO":"O","AGE (years)":"59","RACE":"CAU","SEX":"F","DONOR HEIGHT (cm)":"165","DONOR WEIGHT (kg)":"63","Hep C Positive Donor":"NO","Cold Ischemia (min)":"323","Cold Ischemia (Hrs)":"5.38","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"115333",
"Factors":{"DONOR CAUSE OF DEATH":"Cerebrovascular/Stroke"},
"Additional sample data":{"RECOVERY DATE":"4/10/2015","OPO":"PADV","ABO":"B","AGE (years)":"44","RACE":"BLK","SEX":"M","DONOR HEIGHT (cm)":"180.34","DONOR WEIGHT (kg)":"59","Hep C Positive Donor":"NO","Cold Ischemia (min)":"454","Cold Ischemia (Hrs)":"7.57","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"115342",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"4/10/2015","OPO":"LAOP","ABO":"B","AGE (years)":"46","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"180.34","DONOR WEIGHT (kg)":"103.78","Hep C Positive Donor":"NO","Cold Ischemia (min)":"216","Cold Ischemia (Hrs)":"3.60","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"117751",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"6/25/2015","OPO":"LAOP","ABO":"O","AGE (years)":"48","RACE":"BLK","SEX":"M","DONOR HEIGHT (cm)":"182.9","DONOR WEIGHT (kg)":"105.9","Hep C Positive Donor":"NO","Cold Ischemia (min)":"228","Cold Ischemia (Hrs)":"3.80","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121063",
"Factors":{"DONOR CAUSE OF DEATH":"Cerebrovascular/Stroke"},
"Additional sample data":{"RECOVERY DATE":"8/26/2015","OPO":"LAOP","ABO":"B","AGE (years)":"48","RACE":"BLK","SEX":"F","DONOR HEIGHT (cm)":"154.94","DONOR WEIGHT (kg)":"119.15","Hep C Positive Donor":"NO","Cold Ischemia (min)":"207","Cold Ischemia (Hrs)":"3.45","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"120237",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"8/5/2015","OPO":"LAOP","ABO":"O","AGE (years)":"40","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"177.8","DONOR WEIGHT (kg)":"82.8","Hep C Positive Donor":"NO","Cold Ischemia (min)":"320","Cold Ischemia (Hrs)":"5.33","DCD DONOR":"YES","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"120240",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"8/6/2015","OPO":"TNDS","ABO":"AB","AGE (years)":"27","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"183","DONOR WEIGHT (kg)":"93.3","Hep C Positive Donor":"NO","Cold Ischemia (min)":"293","Cold Ischemia (Hrs)":"4.88","DCD DONOR":"YES","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"120257",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"8/15/2015","OPO":"ALOB","ABO":"O","AGE (years)":"28","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"183","DONOR WEIGHT (kg)":"78","Hep C Positive Donor":"YES","Cold Ischemia (min)":"226","Cold Ischemia (Hrs)":"3.77","DCD DONOR":"YES","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121095",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"9/22/2015","OPO":"LAOP","ABO":"O","AGE (years)":"23","RACE":"BLK","SEX":"F","DONOR HEIGHT (cm)":"170.18","DONOR WEIGHT (kg)":"61.37","Hep C Positive Donor":"NO","Cold Ischemia (min)":"171","Cold Ischemia (Hrs)":"2.85","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"114300",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"1/31/2015","OPO":"LAOP","ABO":"A","AGE (years)":"25","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"182.88","DONOR WEIGHT (kg)":"66.22","Hep C Positive Donor":"NO","Cold Ischemia (min)":"377","Cold Ischemia (Hrs)":"6.28","DCD DONOR":"NO","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"116736",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"4/11/2015","OPO":"LAOP","ABO":"B","AGE (years)":"25","RACE":"BLK","SEX":"F","DONOR HEIGHT (cm)":"139.7","DONOR WEIGHT (kg)":"84.14","Hep C Positive Donor":"NO","Cold Ischemia (min)":"281","Cold Ischemia (Hrs)":"4.68","DCD DONOR":"NO","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121087",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"9/18/2015","OPO":"LAOP","ABO":"O","AGE (years)":"26","RACE":"BLK","SEX":"M","DONOR HEIGHT (cm)":"175.26","DONOR WEIGHT (kg)":"74.84","Hep C Positive Donor":"NO","Cold Ischemia (min)":"366","Cold Ischemia (Hrs)":"6.10","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"117323",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"6/8/2015","OPO":"LAOP","ABO":"O","AGE (years)":"27","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"177.8","DONOR WEIGHT (kg)":"67.8","Hep C Positive Donor":"NO","Cold Ischemia (min)":"262","Cold Ischemia (Hrs)":"4.37","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121091",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"9/20/2015","OPO":"LAOP","ABO":"O","AGE (years)":"31","RACE":"CAU","SEX":"F","DONOR HEIGHT (cm)":"172.72","DONOR WEIGHT (kg)":"77.65","Hep C Positive Donor":"NO","Cold Ischemia (min)":"414","Cold Ischemia (Hrs)":"6.90","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121089",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"9/19/2015","OPO":"LAOP","ABO":"O","AGE (years)":"34","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"182.88","DONOR WEIGHT (kg)":"95","Hep C Positive Donor":"NO","Cold Ischemia (min)":"46","Cold Ischemia (Hrs)":"0.77","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"116709",
"Factors":{"DONOR CAUSE OF DEATH":"Trauma"},
"Additional sample data":{"RECOVERY DATE":"2/17/2015","OPO":"LAOP","ABO":"O","AGE (years)":"35","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"182.88","DONOR WEIGHT (kg)":"72.57","Hep C Positive Donor":"NO","Cold Ischemia (min)":"367","Cold Ischemia (Hrs)":"6.12","DCD DONOR":"NO","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"114647",
"Factors":{"DONOR CAUSE OF DEATH":"Cerebrovascular/Stroke"},
"Additional sample data":{"RECOVERY DATE":"2/12/2015","OPO":"LAOP","ABO":"O","AGE (years)":"39","RACE":"BLK","SEX":"F","DONOR HEIGHT (cm)":"170.18","DONOR WEIGHT (kg)":"80.83","Hep C Positive Donor":"NO","Cold Ischemia (min)":"156","Cold Ischemia (Hrs)":"2.60","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"118601",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"7/19/2015","OPO":"LAOP","ABO":"A1","AGE (years)":"39","RACE":"CAU","SEX":"F","DONOR HEIGHT (cm)":"154.9","DONOR WEIGHT (kg)":"62.5","Hep C Positive Donor":"NO","Cold Ischemia (min)":"236","Cold Ischemia (Hrs)":"3.93","DCD DONOR":"NO","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121079",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"9/8/2015","OPO":"LAOP","ABO":"O","AGE (years)":"40","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"160","DONOR WEIGHT (kg)":"67.2","Hep C Positive Donor":"NO","Cold Ischemia (min)":"338","Cold Ischemia (Hrs)":"5.63","DCD DONOR":"NO","PHS Increased Risk Donor":"YES","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121364",
"Factors":{"DONOR CAUSE OF DEATH":"CVA"},
"Additional sample data":{"RECOVERY DATE":"10/7/2015","OPO":"LAOP","ABO":"O","AGE (years)":"43","RACE":"CAU","SEX":"M","DONOR HEIGHT (cm)":"172.72","DONOR WEIGHT (kg)":"82.9","Hep C Positive Donor":"NO","Cold Ischemia (min)":"54","Cold Ischemia (Hrs)":"0.90","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121358",
"Factors":{"DONOR CAUSE OF DEATH":"CVA"},
"Additional sample data":{"RECOVERY DATE":"10/4/2015","OPO":"LAOP","ABO":"O","AGE (years)":"44","RACE":"BLK","SEX":"F","DONOR HEIGHT (cm)":"162.6","DONOR WEIGHT (kg)":"53.9","Hep C Positive Donor":"NO","Cold Ischemia (min)":"246","Cold Ischemia (Hrs)":"4.10","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"121356",
"Factors":{"DONOR CAUSE OF DEATH":"CVA"},
"Additional sample data":{"RECOVERY DATE":"10/3/2015","OPO":"LAOP","ABO":"O","AGE (years)":"49","RACE":"BLK","SEX":"M","DONOR HEIGHT (cm)":"167.6","DONOR WEIGHT (kg)":"52.4","Hep C Positive Donor":"NO","Cold Ischemia (min)":"336","Cold Ischemia (Hrs)":"5.60","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
},
{
"Subject ID":"-",
"Sample ID":"115347",
"Factors":{"DONOR CAUSE OF DEATH":"Anoxia"},
"Additional sample data":{"RECOVERY DATE":"4/23/2015","OPO":"LAOP","ABO":"B","AGE (years)":"49","RACE":"BLK","SEX":"F","DONOR HEIGHT (cm)":"162.56","DONOR WEIGHT (kg)":"73.84","Hep C Positive Donor":"NO","Cold Ischemia (min)":"280","Cold Ischemia (Hrs)":"4.67","DCD DONOR":"NO","PHS Increased Risk Donor":"NO","Hepatits B Core Ab +":"NO"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"A wedge biopsy of the right lobe of the liver is routinely obtained prior to transplantation. The sample is immediately flash frozen in the operating room and subsequently transferred to Ochsner Biobank for sample storage.","SAMPLE_TYPE":"Liver Tissue","COLLECTION_METHOD":"Open biopsy during cold storage preservation of organ for transplant","COLLECTION_LOCATION":"Operating room of liver transplant during","COLLECTION_FREQUENCY":"Single sample prior to transplant","COLLECTION_DURATION":"Not applicable","COLLECTION_TIME":"Not applicable","VOLUMEORAMOUNT_COLLECTED":"~50mg","STORAGE_CONDITIONS":"-180 degree C, liquid nitrogen vapor phase","COLLECTION_VIALS":"ThermoScientific CryoTube vials, 1.8mL","STORAGE_VIALS":"ThermoScientific CryoTube vials, 1.8mL","COLLECTION_TUBE_TEMP":"-70 degree liguid nitrogen flash freezing","ADDITIVES":"None","BLOOD_SERUM_OR_PLASMA":"N/A","TISSUE_CELL_IDENTIFICATION":"N/A","TISSUE_CELL_QUANTITY_TAKEN":"N/A"},

"TREATMENT":{"TREATMENT_SUMMARY":"Not Applicable","TREATMENT_PROTOCOL_ID":"Not Applicable","TREATMENT_PROTOCOL_FILENAME":"Not Applicable","TREATMENT_PROTOCOL_COMMENTS":"Not Applicable","TREATMENT":"Not Applicable","TREATMENT_COMPOUND":"Not Applicable","TREATMENT_ROUTE":"Not Applicable","TREATMENT_DOSE":"Not Applicable","TREATMENT_DOSEVOLUME":"Not Applicable","TREATMENT_DOSEDURATION":"Not Applicable","TREATMENT_VEHICLE":"Not Applicable"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Amino acid assay|CoA assay|NAD-ATP assay|NADH assay|Organic acid assay","SAMPLEPREP_PROTOCOL_ID":"SB_AA_Assay.pdf;SB_CoA_Assay.pdf;SB_NAD-ATP_Assay_Agilent.pdf;SB_NADH_Assay_Agilent.pdf;SB_OA_Assay.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"NAD-ATP assay","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"Thermo Hypercarb (50 x 2.1mm, 3um)","METHODS_FILENAME":"SB_NAD-ATP_Assay_Agilent.pdf"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6490 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"NAD-ATP assay-ESI"},

"MS_METABOLITE_DATA":{
"Units":"nmol/mg dry powder",

"Data":[{"Metabolite":"AMP","114291":"2.29","116707":"7.17","115165":"0.51","115333":"9.06","115342":"2.38","117751":"5.06"},{"Metabolite":"ADP","114291":"2.62","116707":"2.43","115165":"0.33","115333":"2.50","115342":"1.62","117751":"2.57"},{"Metabolite":"ATP","114291":"0.39","116707":"0.81","115165":"","115333":"0.38","115342":"0.74","117751":"0.72"}],

"Metabolites":[{"Metabolite":"AMP","retention index":"1.51","quantified m/z":"348.1","PubChem ID":"6083","KEGG ID":""},{"Metabolite":"ADP","retention index":"1.51","quantified m/z":"428","PubChem ID":"6022","KEGG ID":""},{"Metabolite":"ATP","retention index":"1.5","quantified m/z":"508.1","PubChem ID":"5957","KEGG ID":""}]
}

}