{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000376","ANALYSIS_ID":"AN000610","VERSION":"1","CREATED_ON":"April 19, 2016, 3:19 pm"},

"PROJECT":{"PROJECT_TITLE":"Role of kidney acetylcholine in protection from kidney injury","PROJECT_SUMMARY":"ChAT was induced in a model of kidney inflammation to determine its role in kidney protection","INSTITUTE":"University of Colorado, Denver","DEPARTMENT":"Medicine","LABORATORY":"Garcia Lab","LAST_NAME":"Garcia","FIRST_NAME":"Gabriela","ADDRESS":"Denver, CO","EMAIL":"Gabriela.garcia@ucdenver.edu","PHONE":"303-724-4897"},

"STUDY":{"STUDY_TITLE":"Kidney choline acetyltransferase (ChAT)-3","STUDY_TYPE":"Regular","STUDY_SUMMARY":"We use ChAT(BAC)-EGFP mice, which express enhanced green fluorescent protein (EGFP) under the control of transcriptional regulatory elements for choline acetyltransferase (ChAT), the sole enzyme that catalyzes the biosynthesis of acetylcholine (ACh). These mice were treated with inducers of ChAT. Kidney were rapidly removed, frozen on liquid nitrogen and stored at -80C","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"7","TOTAL_SUBJECTS":"25"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0012166",
"Sample ID":"S00018085",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018086",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018087",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018088",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018089",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018090",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018091",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018092",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018093",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018094",
"Factors":{"Treated":"Vehicle","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018095",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018096",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018097",
"Factors":{"Treated":"Vehicle","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018098",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018099",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018100",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018101",
"Factors":{"Treated":"Control","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018102",
"Factors":{"Treated":"Control","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012167",
"Sample ID":"S00018103",
"Factors":{"Treated":"Control","Gender":"Female","Strain":"WT"}
},
{
"Subject ID":"SU0012167",
"Sample ID":"S00018104",
"Factors":{"Treated":"Control","Gender":"Female","Strain":"WT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018105",
"Factors":{"Treated":"Treated","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018106",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018107",
"Factors":{"Treated":"Treated","Gender":"Female","Strain":"ChAT"}
},
{
"Subject ID":"SU0012166",
"Sample ID":"S00018108",
"Factors":{"Treated":"Control","Gender":"Male","Strain":"ChAT"}
},
{
"Subject ID":"SU0012167",
"Sample ID":"S00018109",
"Factors":{"Treated":"Control","Gender":"Male","Strain":"WT"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"none","SAMPLE_TYPE":"Kidney"},

"TREATMENT":{"TREATMENT_SUMMARY":"none"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"none","SAMPLEPREP_PROTOCOL_FILENAME":"2014-10-07-Neurotransmitter.docx"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"None","INSTRUMENT_NAME":"Unspecified","COLUMN_NAME":"Cogent Diamond Hydride","METHODS_FILENAME":"QM009_2mm_DH_Neurotransmitter_MRM-ESP-1.m.zip","METHODS_ID":"DQM0150"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6410A QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/mg sample",

"Data":[{"Metabolite":"5-Hydroxyindoleacetic acid","S00018085":"54.35","S00018086":"60.32","S00018087":"11.86","S00018088":"137.95","S00018089":"41.64","S00018090":"15.35","S00018091":"32.93","S00018092":"78.95","S00018093":"17.52","S00018094":"21.21","S00018095":"39.39","S00018096":"22.83","S00018097":"37.21","S00018098":"87.97","S00018099":"117.58","S00018100":"13.79","S00018101":"23.65","S00018102":"8.53","S00018103":"21.56","S00018104":"16.70","S00018105":"24.86","S00018106":"158.95","S00018107":"10.69","S00018108":"229.03"},{"Metabolite":"Adenosine","S00018085":"215.39","S00018086":"177.95","S00018087":"211.85","S00018088":"208.78","S00018089":"227.71","S00018090":"24.84","S00018091":"148.61","S00018092":"201.35","S00018093":"169.80","S00018094":"154.60","S00018095":"227.84","S00018096":"158.49","S00018097":"433.38","S00018098":"383.69","S00018099":"350.31","S00018100":"283.57","S00018101":"290.94","S00018102":"175.44","S00018103":"232.07","S00018104":"161.29","S00018105":"243.21","S00018106":"358.18","S00018107":"222.07","S00018108":"322.47"},{"Metabolite":"Serotonin","S00018085":"1.59","S00018086":"1.45","S00018087":"1.51","S00018088":"1.51","S00018089":"1.50","S00018090":"1.30","S00018091":"1.58","S00018092":"1.54","S00018093":"1.64","S00018094":"0.00","S00018095":"1.53","S00018096":"1.39","S00018097":"1.62","S00018098":"1.45","S00018099":"1.43","S00018100":"1.33","S00018101":"1.32","S00018102":"1.28","S00018103":"1.30","S00018104":"1.32","S00018105":"1.51","S00018106":"1.54","S00018107":"1.61","S00018108":"1.65"},{"Metabolite":"3-Methoxytyramine","S00018085":"0.76","S00018086":"0.77","S00018087":"0.77","S00018088":"0.76","S00018089":"0.76","S00018090":"0.79","S00018091":"0.76","S00018092":"0.78","S00018093":"0.82","S00018094":"0.00","S00018095":"0.78","S00018096":"0.80","S00018097":"0.77","S00018098":"0.77","S00018099":"0.77","S00018100":"0.77","S00018101":"0.76","S00018102":"0.76","S00018103":"0.76","S00018104":"0.76","S00018105":"0.78","S00018106":"0.76","S00018107":"0.78","S00018108":"0.77"},{"Metabolite":"Acetylcholine","S00018085":"2.77","S00018086":"2.48","S00018087":"6.08","S00018088":"1.93","S00018089":"2.95","S00018090":"5.77","S00018091":"3.55","S00018092":"3.26","S00018093":"2.72","S00018094":"1.36","S00018095":"15.67","S00018096":"2.01","S00018097":"2.35","S00018098":"19.49","S00018099":"10.77","S00018100":"2.73","S00018101":"2.96","S00018102":"1.50","S00018103":"0.00","S00018104":"0.00","S00018105":"2.47","S00018106":"3.36","S00018107":"13.08","S00018108":"6.68"}],

"Metabolites":[{"Metabolite":"5-Hydroxyindoleacetic acid","retention index":"","quantified m/z":"","PubChem ID":"1826","KEGG ID":""},{"Metabolite":"Adenosine","retention index":"","quantified m/z":"","PubChem ID":"60961","KEGG ID":""},{"Metabolite":"Serotonin","retention index":"","quantified m/z":"","PubChem ID":"5202","KEGG ID":""},{"Metabolite":"3-Methoxytyramine","retention index":"","quantified m/z":"","PubChem ID":"1669","KEGG ID":""},{"Metabolite":"Acetylcholine","retention index":"","quantified m/z":"","PubChem ID":"187","KEGG ID":""}]
}

}