{ "METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000428","ANALYSIS_ID":"AN000678","VERSION":"1","CREATED_ON":"July 25, 2016, 2:09 pm"}, "PROJECT":{"PROJECT_TITLE":"Lung injury-induced skeletal muscle wasting in aged mice is linked to alterations in long chain fatty acid metabolism","PROJECT_TYPE":"Metabolomics","PROJECT_SUMMARY":"Non targeted and targeted metabolomic analysis on gastrocnemius tissue samples obtained from skeletal muscle of adult and old mice after inducing lung injury","INSTITUTE":"University of North Carolina","DEPARTMENT":"McAllister Heart Institute, Department of Internal Medicine","LABORATORY":"Multiple Centers","LAST_NAME":"Willis, Ilaiwy","FIRST_NAME":"Monte, Amro","ADDRESS":"111 Mason Farm road, Chapel Hill, North Carolina, 27599-7126, USA","EMAIL":"monte_willis@med.unc.edu, amroilaiwy@gmail.com","PHONE":"210-596-0171","FUNDING_SOURCE":"NIH, Fondation Leducq, Claude D. Pepper Older Americans Independence Center, the American Thoracic Society Foundation"}, "STUDY":{"STUDY_TITLE":"Targeted metabolomics of gastrocnemius tissue samples obtained from 20 month old (old) mice- Both Sham and after inducing lung injury (part II)","STUDY_TYPE":"Targeted metabolomic analysis","STUDY_SUMMARY":"Introduction: Older patients are more likely to acquire and die from acute respiratory distress syndrome (ARDS) and muscle weakness may be more significant in older survivors. Recent data implicate muscle ring finger protein 1 (MuRF1) in lung injury-induced skeletal muscle atrophy in young mice and identify an alternative role for MuRF1 in cardiac metabolism regulation through inhibition of fatty acid oxidation. Objectives: To develop a model of lung injury-induced muscle wasting in old mice and to evaluate the skeletal muscle metabolomic profile of adult and old acute lung injury (ALI) mice. Methods: Young (2 month), adult (6 month) and old (20 month) male C57Bl6J mice underwent Sham (intratracheal H2O) or ALI [intratracheal E. coli lipopolysaccharide (i.t. LPS)] conditions and muscle functional testing. Metabolomic analysis on gastrocnemius muscle was performed using gas chromatography-mass spectrometry (GC-MS). Results: Old ALI mice had increased mortality and failed to recover skeletal muscle function compared to adult ALI mice. Muscle MuRF1 expression was increased in old ALI mice at day 3. Non-targeted muscle metabolomics revealed alterations in amino acid biosynthesis and fatty acid metabolism in old ALI mice. Targeted metabolomics of fatty acid intermediates (acyl-carnitines) and amino acids revealed a reduction in long chain acyl-carnitines in old ALI mice. Conclusion: This study demonstrates age-associated susceptibility to ALI-induced muscle wasting which parallels a metabolomic profile suggestive of altered muscle fatty acid metabolism. MuRF1 activation may contribute to both atrophy and impaired fatty acid oxidation, which may synergistically impair muscle function in old ALI mice.","INSTITUTE":"University of North Carolina, Duke University","DEPARTMENT":"UNC McAllister Heart Institute, Duke Molecular Physiology Institute","LABORATORY":"Multiple Centers","LAST_NAME":"Willis, Ilaiwy","FIRST_NAME":"Monte, Amro","ADDRESS":"111 Mason Farm road, Chapel Hill, North Carolina, 27599-7126, USA","EMAIL":"monte_willis@med.unc.edu, amroilaiwy@gmail.com","PHONE":"210-596-0171"}, "SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"}, "SUBJECT_SAMPLE_FACTORS":[ { "Subject ID":"-", "Sample ID":"A08", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A09", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A10", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A11", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A12", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A32", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A33", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A34", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A50", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A52", "Factors":{"Sample":"Sham 20mth"} }, { "Subject ID":"-", "Sample ID":"A05", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A106", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A108", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A109", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A110", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A111", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A45", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A46", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A47", "Factors":{"Sample":"ALI 20mth"} }, { "Subject ID":"-", "Sample ID":"A48", "Factors":{"Sample":"ALI 20mth"} } ], "COLLECTION":{"COLLECTION_SUMMARY":"Gastrocnemius tissue was harvested and then flash frozen in a liquid nitrogen cooled biopress"}, "TREATMENT":{"TREATMENT_SUMMARY":"Treatment Summary Fraction of gastrocnemius tissue weighed (25–50 mg wet weight), then the finely cut up tissue quickly added to fresh pre-made buffer (50 % acetyl-nitrile, 50 % water, 0.3% formic acid) at a standard concentration of 25 mg/475 mcl buffer then fully homogenized on ice for 10–25 s and placed on dry ice/stored at - 80C"}, "SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Amino acids and acyl-carnitines were analyzed using stable isotope dilution techniques. Amino acids and acyl-carnitine measurements were made by flow injection tandem mass spectrometry"}, "CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"ACQUITY UPLC 1.7 µm column"}, "ANALYSIS":{"ANALYSIS_TYPE":"MS"}, "MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE"}, "MS_METABOLITE_DATA":{ "Units":"uM", 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