{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000449","ANALYSIS_ID":"AN000704","VERSION":"1","CREATED_ON":"August 18, 2016, 1:51 pm"},

"PROJECT":{"PROJECT_TITLE":"Molecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry","PROJECT_SUMMARY":"This study utilized laser ablation electrospray ionization mass spectrometry imaging to study the metabolism and antibiotic inhibition in bacterial colonies.","INSTITUTE":"The George Washington University","DEPARTMENT":"Chemistry","LABORATORY":"Vertes Research Lab","LAST_NAME":"Li","FIRST_NAME":"Hang","ADDRESS":"800 22nd St. NW, Suite 4000, Washington, DC 20052","EMAIL":"amy_li@gwmail.gwu.edu","PHONE":"202-994-6344"},

"STUDY":{"STUDY_TITLE":"Molecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry","STUDY_SUMMARY":"This study investigates the metabolism and antibiotic inhibition in Escherichia coli and Bacillus subtilis colonies.","INSTITUTE":"George Washington University","DEPARTMENT":"Chemistry","LABORATORY":"Vertes Research Lab","LAST_NAME":"Li","FIRST_NAME":"Hang","ADDRESS":"800 22nd St. NW, Suite 4000, Washington, DC 20052","EMAIL":"amy_li@gwmail.gwu.edu","PHONE":"202-994-6344"},

"SUBJECT":{"SUBJECT_TYPE":"Microbial (Escherichia coli, bacillus subtilis)","SUBJECT_SPECIES":"Bacillus subtilis","TAXONOMY_ID":"1423","GENOTYPE_STRAIN":"ATCC12435, ATCC6051"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"EC-1",
"Factors":{"Treatment":"Blank"}
},
{
"Subject ID":"-",
"Sample ID":"BS-1",
"Factors":{"Treatment":"Blank"}
},
{
"Subject ID":"-",
"Sample ID":"BS-2",
"Factors":{"Treatment":"Streptomycin-penicillin"}
},
{
"Subject ID":"-",
"Sample ID":"ECBS-1",
"Factors":{"Treatment":"Streptomycin"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"All the sample were analyzed using laser ablation electrospray ionization (LAESI) MS or MSI."},

"TREATMENT":{"TREATMENT_SUMMARY":"In the antibiotic susceptibility testing, the bacterial colonies were treated with streptomycin or streptomycin-penicillin."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The E. coli and B. subtilis colonies were inoculated and incubated following the standard protocol. The antibiotic inhibition tests were performed according to the conventional Kirby-Bauer antibiotic testing."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"No chromatography was used for this study","CHROMATOGRAPHY_TYPE":"None","INSTRUMENT_NAME":"Synapt G2S","COLUMN_NAME":"None"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Waters Synapt G2 S QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"m/z",

"Data":[{"Metabolite":"Acetylspermidine","EC-1":"188.176","BS-1":"188.177"},false,{"Metabolite":"Valine","EC-1":"118.085","BS-1":"118.087"},{"Metabolite":"Hypoxanthine","EC-1":"137.048","BS-1":"137.046"},{"Metabolite":"S-formylglutathione","EC-1":"374.039","BS-1":"374.037"}],

"Metabolites":[{"Metabolite":"Acetylspermidine","retention index":"","quantified m/z":"","PubChem ID":"","KEGG ID":"C00612"},{"Metabolite":"Glutathione","retention index":"","quantified m/z":"","PubChem ID":"","KEGG ID":"C00051"},{"Metabolite":"Valine","retention index":"","quantified m/z":"","PubChem ID":"","KEGG ID":"C00183"},{"Metabolite":"Hypoxanthine","retention index":"","quantified m/z":"","PubChem ID":"","KEGG ID":"C00262"},{"Metabolite":"S-formylglutathione","retention index":"","quantified m/z":"","PubChem ID":"","KEGG ID":"C01031"}]
}

}