{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000450","ANALYSIS_ID":"AN000705","VERSION":"1","CREATED_ON":"August 19, 2016, 4:17 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolic features of chronic fatigue","PROJECT_TYPE":"Human plasma","PROJECT_SUMMARY":"More than 2 million people in the United States have myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). We performed targeted, broad-spectrum metabolomics to gain insights into the biology of CFS. We studied a total of 84 subjects using these methods. Forty-five subjects (n = 22 men and 23 women) met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria. We targeted 612 metabolites in plasma from 63 biochemical pathways by hydrophilic interaction liquid chromatography, electrospray ionization, and tandem mass spectrometry in a single-injection method. Our data show that despite the heterogeneity of factors leading to CFS, the cellular metabolic response in patients was homogeneous, statistically robust, and chemically similar to the evolutionarily conserved persistence response to environmental stress known as dauer.","INSTITUTE":"University of California, San Diego","DEPARTMENT":"The Mitochondrial and Metabolic Disease Center","LAST_NAME":"Naviaux","FIRST_NAME":"Robert","ADDRESS":"214 Dikinson Street, CTF-C102, San Diego, CA, 92103","EMAIL":"maviaux@ucsd.edu","PHONE":"(619) 993-2904","FUNDING_SOURCE":"UCSD Christini Fund,The Wright Family Foundation,The Lennox Foundation, The It Takes Guts Foundation, The UCSD Mitochondrial Disease Research Fund"},

"STUDY":{"STUDY_TITLE":"Metabolic features of chronic fatigue syndrome","STUDY_TYPE":"Plasma metabolomic profiling","STUDY_SUMMARY":"This targeted metabolomic analysis was performed on plasma samples from 39 normal controls (n=18 men and 21 women) and 45 subjects ((n = 22 men and 23 women) who met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria.","INSTITUTE":"University of California, San Diego","DEPARTMENT":"The Mitochondrial and Metabolic Disease Center","LAST_NAME":"Naviaux","FIRST_NAME":"Robert","ADDRESS":"214 Dikinson Street, CTF-C102, San Diego, CA, 92103","EMAIL":"maviaux@ucsd.edu","PHONE":"619-993-2904","NUM_GROUPS":"2 groups for men (control and CFS) and 2 groups for women (control and CFS)","TOTAL_SUBJECTS":"84"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"Males with CFS were 53 (±2.8) y old (mean±SEM; range, 21–67 y). Females were 52 (±2.5) y old (range, 20–67 y).","GENDER":"23 females and 22 males with CFS and 18 male and 21 female controls.","HUMAN_ETHNICITY":"White/non-Hispanic"},
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"Factors":{"Disease":"CFS","gender":"Female"}
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],
"COLLECTION":{"COLLECTION_SUMMARY":"None","SAMPLE_TYPE":"Blood","COLLECTION_METHOD":"Lithium-heparin vacutainer tubes","STORAGE_CONDITIONS":"-80C","BLOOD_SERUM_OR_PLASMA":"Plasma"},

"TREATMENT":{"TREATMENT_SUMMARY":"No treatment"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"None","EXTRACTION_METHOD":"Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis.","EXTRACT_STORAGE":"-80°C","SAMPLE_DERIVATIZATION":"No","SAMPLE_SPIKING":"25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"HILIC","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Shimadzu 20AD","COLUMN_NAME":"Shodex NH2P-40 2E (250 mm × 2.0 mm)","FLOW_GRADIENT":"0-3.5 min: 95%B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 13.5–35 min: 0% B, 36–46 min: 95% B, 46.1 min: end.","FLOW_RATE":"0.2 ml/min","COLUMN_TEMPERATURE":"25C","SOLVENT_A":"95% water with 20 mM (NH4)2CO3, 5% acetonitrile, and 38 mM NH4OH, final pH 9.75","SOLVENT_B":"100% acetonitrile","COLUMN_PRESSURE":"920~2600 psi","ANALYTICAL_TIME":"46.1 min","OVEN_TEMPERATURE":"25C","SAMPLE_LOOP_SIZE":"10 ul","SAMPLE_SYRINGE_SIZE":"100 ul"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","DETECTOR_TYPE":"MS/MS","SOFTWARE_VERSION":"Analyst 1.6.2"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"ABI Sciex 5500 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE","COLLISION_ENERGY":"Compound-specific","COLLISION_GAS":"High","ION_SOURCE_TEMPERATURE":"500C","ION_SPRAY_VOLTAGE":"5500V","IONIZATION":"ESI","MASS_ACCURACY":"0.1Da"},

"MS_METABOLITE_DATA":{
"Units":"AUC",

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