{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000518","ANALYSIS_ID":"AN000792","VERSION":"1","CREATED_ON":"December 13, 2016, 12:24 pm"},

"PROJECT":{"PROJECT_TITLE":"Mayo Metabolomics Pilot and Feasibility Award: Role of muscle insulin and IGF-1 signaling on serum and muscle metabolite profiles","PROJECT_SUMMARY":"Skeletal muscle insulin resistance is a cardinal feature of the pathogenesis of type 2 diabetes. Insulin and IGF-1 signal through their highly related receptors to impact on many aspects of muscle physiology including glucose homeostasis, protein metabolism, and mitochondrial function. Early physiological studies, as well as recent large scale metabolomic studies, have shown that changes in specific pools of circulating amino acid metabolites, such as branched chain amino acids (BCAAs), are associated with insulin resistance and can predict future diabetes, but the source and impact of these changes in amino acids are not fully understood. We have recently generated mice which lack insulin receptors (IR) or IGF-1 receptors (IGF1R) or both in muscle using Cre lox recombination. We find that mice which lack only IR or only IGF1R in muscle show minimal changes in muscle mass, but do display increases in proteasomal activity and autophagy in muscle. On the other hand, mice with combined loss of both IR and IGF1R display markedly decreased muscle mass and enhanced degradation pathways, associated with increased protein synthesis, and display changes in mitochondrial gene regulation, indicating that both receptors can compensate to some extent for loss of the other. We hypothesize that IR and IGF1R signaling in muscle coordinate amino acid metabolite turnover and fuel substrate/mitochondrial metabolism, and that in insulin resistant states, changes in protein metabolism and mitochondrial function disrupt relative proportions of amino acid metabolites, which in turn contribute to diabetes risk and/or muscle pathology. We propose to test this hypothesis by performing large scale metabolomics on serum and muscle from mice lacking IR, IGF1R or both in muscle, and we will compare these changes to both insulin deficient streptozotocin-treated and insulin resistant diet-induced obese mouse models. To gain insight into which pathways are critical for metabolite changes, we will also treat mice with specific inhibitors of mTOR, a common protein synthesis pathway, as well as inhibitors of autophagy or proteasomal degradation and determine metabolite concentrations in muscle and serum. These studies will identify specific pathways that impact amino acid and mitochondrial metabolite flux which are perturbed in insulin resistant states, and potentially provide insights into how changes in amino acid metabolites contribute to diabetes risk.","INSTITUTE":"Mayo Clinic","LAST_NAME":"O'Neill","FIRST_NAME":"Brian","ADDRESS":"One Joslin Place, Boston, MA 02215","EMAIL":"brian.o'neill@joslin.harvard.edu","PHONE":"617-309-2400"},

"STUDY":{"STUDY_TITLE":"Measuring ceramide concentrations in insulin resistant and insulin deficient mouse tissue models","STUDY_SUMMARY":"To compare models of insulin resistance to a model of loss of insulin signaling, we will also determine ceramide concentration in muscle, using control and streptozotocin (STZ) treated mice as a model of insulin deficient diabetes.","INSTITUTE":"Mayo Clinic","LAST_NAME":"O'Neill","FIRST_NAME":"Brian","ADDRESS":"One Joslin Place, Boston, MA 02215","EMAIL":"brian.o'neill@joslin.harvard.edu","PHONE":"617-309-2400"},

"SUBJECT":{"SUBJECT_TYPE":"Mouse","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"F467",
"Sample ID":"ms5632-1",
"Factors":{"group":"control"},
"Additional sample data":{"age":"7.9","tissue weight (mg)":"32.51"}
},
{
"Subject ID":"F471",
"Sample ID":"ms5632-2",
"Factors":{"group":"control"},
"Additional sample data":{"age":"7.0","tissue weight (mg)":"38.4"}
},
{
"Subject ID":"2095",
"Sample ID":"ms5632-3",
"Factors":{"group":"control"},
"Additional sample data":{"age":"9.6","tissue weight (mg)":"49.05"}
},
{
"Subject ID":"2103",
"Sample ID":"ms5632-4",
"Factors":{"group":"control"},
"Additional sample data":{"age":"8.3","tissue weight (mg)":"53.96"}
},
{
"Subject ID":"MF1092",
"Sample ID":"ms5632-5",
"Factors":{"group":"control"},
"Additional sample data":{"age":"9.7","tissue weight (mg)":"47.92"}
},
{
"Subject ID":"MF1103",
"Sample ID":"ms5632-6",
"Factors":{"group":"control"},
"Additional sample data":{"age":"8.7","tissue weight (mg)":"54.03"}
},
{
"Subject ID":"2096",
"Sample ID":"ms5632-7",
"Factors":{"group":"MIGIRKO"},
"Additional sample data":{"age":"9.6","tissue weight (mg)":"46.6"}
},
{
"Subject ID":"2102",
"Sample ID":"ms5632-8",
"Factors":{"group":"MIGIRKO"},
"Additional sample data":{"age":"8.3","tissue weight (mg)":"48.1"}
},
{
"Subject ID":"2109",
"Sample ID":"ms5632-9",
"Factors":{"group":"MIGIRKO"},
"Additional sample data":{"age":"8.6","tissue weight (mg)":"40.8"}
},
{
"Subject ID":"2111",
"Sample ID":"ms5632-10",
"Factors":{"group":"MIGIRKO"},
"Additional sample data":{"age":"8.6","tissue weight (mg)":"45.7"}
},
{
"Subject ID":"2064",
"Sample ID":"ms5632-11",
"Factors":{"group":"MIGIRKO"},
"Additional sample data":{"age":"10.1","tissue weight (mg)":"29.4"}
},
{
"Subject ID":"MF991",
"Sample ID":"ms5632-12",
"Factors":{"group":"MIGIRKO"},
"Additional sample data":{"age":"9.4","tissue weight (mg)":"37.1"}
},
{
"Subject ID":"F453",
"Sample ID":"ms5632-13",
"Factors":{"group":"Fox0 TKO"},
"Additional sample data":{"age":"9.7","tissue weight (mg)":"44.14"}
},
{
"Subject ID":"F455",
"Sample ID":"ms5632-14",
"Factors":{"group":"Fox0 TKO"},
"Additional sample data":{"age":"9.7","tissue weight (mg)":"57.93"}
},
{
"Subject ID":"F472",
"Sample ID":"ms5632-15",
"Factors":{"group":"Fox0 TKO"},
"Additional sample data":{"age":"7.0","tissue weight (mg)":"47.52"}
},
{
"Subject ID":"F394",
"Sample ID":"ms5632-16",
"Factors":{"group":"Fox0 TKO"},
"Additional sample data":{"age":"13.1","tissue weight (mg)":"51.34"}
},
{
"Subject ID":"F323",
"Sample ID":"ms5632-17",
"Factors":{"group":"Fox0 TKO"},
"Additional sample data":{"age":"9.9","tissue weight (mg)":"41.06"}
},
{
"Subject ID":"MF496",
"Sample ID":"ms5632-18",
"Factors":{"group":"Fox0 TKO"},
"Additional sample data":{"age":"12.9","tissue weight (mg)":"52.07"}
},
{
"Subject ID":"MF1091",
"Sample ID":"ms5632-19",
"Factors":{"group":"QKO"},
"Additional sample data":{"age":"9.7","tissue weight (mg)":"47.77"}
},
{
"Subject ID":"MF1104",
"Sample ID":"ms5632-20",
"Factors":{"group":"QKO"},
"Additional sample data":{"age":"8.7","tissue weight (mg)":"60.11"}
},
{
"Subject ID":"MF1105",
"Sample ID":"ms5632-21",
"Factors":{"group":"QKO"},
"Additional sample data":{"age":"8.7","tissue weight (mg)":"58.32"}
},
{
"Subject ID":"MF1107",
"Sample ID":"ms5632-22",
"Factors":{"group":"QKO"},
"Additional sample data":{"age":"8.7","tissue weight (mg)":"56.07"}
},
{
"Subject ID":"MF1119",
"Sample ID":"ms5632-23",
"Factors":{"group":"QKO"},
"Additional sample data":{"age":"8.5","tissue weight (mg)":"66.63"}
},
{
"Subject ID":"MF1064",
"Sample ID":"ms5632-24",
"Factors":{"group":"QKO"},
"Additional sample data":{"age":"9.0","tissue weight (mg)":"65.87"}
},
{
"Subject ID":"F444",
"Sample ID":"ms5632-25",
"Factors":{"group":"STZ"},
"Additional sample data":{"age":"10.0","tissue weight (mg)":"54.4"}
},
{
"Subject ID":"F463",
"Sample ID":"ms5632-26",
"Factors":{"group":"STZ"},
"Additional sample data":{"age":"8.3","tissue weight (mg)":"39.02"}
},
{
"Subject ID":"F304",
"Sample ID":"ms5632-27",
"Factors":{"group":"STZ"},
"Additional sample data":{"age":"11.9","tissue weight (mg)":"41.38"}
},
{
"Subject ID":"F322",
"Sample ID":"ms5632-28",
"Factors":{"group":"STZ"},
"Additional sample data":{"age":"9.9","tissue weight (mg)":"43.23"}
},
{
"Subject ID":"MF569",
"Sample ID":"ms5632-29",
"Factors":{"group":"STZ"},
"Additional sample data":{"age":"11.4","tissue weight (mg)":"43.99"}
},
{
"Subject ID":"MF1046",
"Sample ID":"ms5632-30",
"Factors":{"group":"STZ"},
"Additional sample data":{"age":"8.9","tissue weight (mg)":"26.92"}
},
{
"Subject ID":"F454",
"Sample ID":"ms5632-31",
"Factors":{"group":"STZ FoxO TKO"},
"Additional sample data":{"age":"9.7","tissue weight (mg)":"48.86"}
},
{
"Subject ID":"F456",
"Sample ID":"ms5632-32",
"Factors":{"group":"STZ FoxO TKO"},
"Additional sample data":{"age":"9.7","tissue weight (mg)":"41.49"}
},
{
"Subject ID":"F462",
"Sample ID":"ms5632-33",
"Factors":{"group":"STZ FoxO TKO"},
"Additional sample data":{"age":"8.3","tissue weight (mg)":"32.12"}
},
{
"Subject ID":"F325",
"Sample ID":"ms5632-34",
"Factors":{"group":"STZ FoxO TKO"},
"Additional sample data":{"age":"9.9","tissue weight (mg)":"44.06"}
},
{
"Subject ID":"MF512",
"Sample ID":"ms5632-35",
"Factors":{"group":"STZ FoxO TKO"},
"Additional sample data":{"age":"12.1","tissue weight (mg)":"44.26"}
},
{
"Subject ID":"MF568",
"Sample ID":"ms5632-36",
"Factors":{"group":"STZ FoxO TKO"},
"Additional sample data":{"age":"11.4","tissue weight (mg)":"57.64"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"To determine the role of FoxO transcription factors in muscle atrophy and increased autophagy in MIGIRKO mice, we crossed MIGIRKO (lacking IR and IGF1R) mice with mice in which FoxO1, FoxO3, and FoxO4 genes were floxed to delete all the major isoforms of FoxO expressed in muscle. Mice in which 5 separate genes — IR, Igf1r, FoxO1, FoxO3, and FoxO4 — were specifically deleted in muscle (muscle quintuple-knockout mice, hereafter referred to as QKO mice); were born in normal Mendelian ratios, and appeared normal both on external inspection and following dissection compared with littermate controls and with muscle FoxO1/3/4 triple-knockout mice (FoxO TKO). Streptozotocin (STZ) treated mice were used as a model of insulin deficient diabetes."},

"TREATMENT":{"TREATMENT_SUMMARY":"To determine the relevance of the changes in M-IR-/-, M-IGF1R-/- , and MIGIRKO mice to insulin resistant states, we will perform large scale metabolomics and determine ceramide concentrations metabolites in muscle and serum from 5 mice fed chow diet or 5 fed a high fat diet (HFD) for 8 weeks. Lastly, to compare models of insulin resistance to a model of loss of insulin signaling, we will also determine muscle and serum metabolites in 5 control and 5 streptozotocin (STZ) treated mice as a model of insulin deficient diabetes."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"ceramide concentrations in muscle tissue"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Waters Acquity BEH C8 (150 x 2mm, 1.7um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo Quantiva QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"ng/mg",

"Data":[{"Metabolite":"Sph","ms5632-1":"0.03","ms5632-2":"0.04","ms5632-3":"0.03","ms5632-4":"0.03","ms5632-5":"0.03","ms5632-6":"0.04","ms5632-7":"0.07","ms5632-8":"0.09","ms5632-9":"0.08","ms5632-10":"0.06","ms5632-11":"0.11","ms5632-12":"0.09","ms5632-13":"0.03","ms5632-14":"0.08","ms5632-15":"0.08","ms5632-16":"0.03","ms5632-17":"0.04","ms5632-18":"0.04","ms5632-19":"0.05","ms5632-20":"0.04","ms5632-21":"0.04","ms5632-22":"0.08","ms5632-23":"0.04","ms5632-24":"0.06","ms5632-25":"0.05","ms5632-26":"0.10","ms5632-27":"0.05","ms5632-28":"0.03","ms5632-29":"0.05","ms5632-30":"0.10","ms5632-31":"0.08","ms5632-32":"0.10","ms5632-33":"0.08","ms5632-34":"0.07","ms5632-35":"0.05","ms5632-36":"0.04"},{"Metabolite":"Spa","ms5632-1":"0.01","ms5632-2":"0.01","ms5632-3":"0.01","ms5632-4":"0.01","ms5632-5":"0.01","ms5632-6":"0.01","ms5632-7":"0.03","ms5632-8":"0.04","ms5632-9":"0.03","ms5632-10":"0.03","ms5632-11":"0.05","ms5632-12":"0.04","ms5632-13":"0.01","ms5632-14":"0.02","ms5632-15":"0.04","ms5632-16":"0.01","ms5632-17":"0.02","ms5632-18":"0.01","ms5632-19":"0.01","ms5632-20":"0.01","ms5632-21":"0.01","ms5632-22":"0.03","ms5632-23":"0.01","ms5632-24":"0.02","ms5632-25":"0.01","ms5632-26":"0.03","ms5632-27":"0.01","ms5632-28":"0.01","ms5632-29":"0.01","ms5632-30":"0.03","ms5632-31":"0.03","ms5632-32":"0.02","ms5632-33":"0.02","ms5632-34":"0.02","ms5632-35":"0.01","ms5632-36":"0.01"},{"Metabolite":"S1P","ms5632-1":"0.01","ms5632-2":"0.01","ms5632-3":"0.01","ms5632-4":"0.01","ms5632-5":"0.01","ms5632-6":"0.01","ms5632-7":"0.02","ms5632-8":"0.02","ms5632-9":"0.02","ms5632-10":"0.01","ms5632-11":"0.04","ms5632-12":"0.04","ms5632-13":"0.01","ms5632-14":"0.01","ms5632-15":"0.01","ms5632-16":"0.01","ms5632-17":"0.01","ms5632-18":"0.01","ms5632-19":"0.00","ms5632-20":"0.00","ms5632-21":"0.00","ms5632-22":"0.01","ms5632-23":"0.00","ms5632-24":"0.01","ms5632-25":"0.01","ms5632-26":"0.01","ms5632-27":"0.01","ms5632-28":"0.01","ms5632-29":"0.01","ms5632-30":"0.01","ms5632-31":"0.01","ms5632-32":"0.01","ms5632-33":"0.01","ms5632-34":"0.01","ms5632-35":"0.01","ms5632-36":"0.00"},{"Metabolite":"C8","ms5632-1":"0.00","ms5632-2":"0.00","ms5632-3":"0.00","ms5632-4":"0.00","ms5632-5":"0.00","ms5632-6":"0.00","ms5632-7":"0.00","ms5632-8":"0.00","ms5632-9":"0.00","ms5632-10":"0.00","ms5632-11":"0.00","ms5632-12":"0.00","ms5632-13":"0.00","ms5632-14":"0.00","ms5632-15":"0.00","ms5632-16":"0.00","ms5632-17":"0.00","ms5632-18":"0.00","ms5632-19":"0.00","ms5632-20":"0.00","ms5632-21":"0.00","ms5632-22":"0.00","ms5632-23":"0.00","ms5632-24":"0.00","ms5632-25":"0.00","ms5632-26":"0.00","ms5632-27":"0.00","ms5632-28":"0.00","ms5632-29":"0.00","ms5632-30":"0.00","ms5632-31":"0.00","ms5632-32":"0.00","ms5632-33":"0.00","ms5632-34":"0.00","ms5632-35":"0.00","ms5632-36":"0.00"},{"Metabolite":"C14","ms5632-1":"0.01","ms5632-2":"0.00","ms5632-3":"0.00","ms5632-4":"0.01","ms5632-5":"0.00","ms5632-6":"0.00","ms5632-7":"0.01","ms5632-8":"0.01","ms5632-9":"0.02","ms5632-10":"0.01","ms5632-11":"0.02","ms5632-12":"0.02","ms5632-13":"0.01","ms5632-14":"0.07","ms5632-15":"0.01","ms5632-16":"0.00","ms5632-17":"0.01","ms5632-18":"0.00","ms5632-19":"0.01","ms5632-20":"0.01","ms5632-21":"0.00","ms5632-22":"0.02","ms5632-23":"0.00","ms5632-24":"0.01","ms5632-25":"0.00","ms5632-26":"0.01","ms5632-27":"0.00","ms5632-28":"0.00","ms5632-29":"0.01","ms5632-30":"0.01","ms5632-31":"0.01","ms5632-32":"0.01","ms5632-33":"0.01","ms5632-34":"0.00","ms5632-35":"0.00","ms5632-36":"0.00"},{"Metabolite":"C16","ms5632-1":"0.43","ms5632-2":"0.50","ms5632-3":"0.35","ms5632-4":"0.71","ms5632-5":"0.44","ms5632-6":"0.43","ms5632-7":"1.15","ms5632-8":"1.32","ms5632-9":"1.54","ms5632-10":"1.90","ms5632-11":"2.25","ms5632-12":"2.04","ms5632-13":"0.38","ms5632-14":"1.51","ms5632-15":"1.13","ms5632-16":"0.42","ms5632-17":"0.49","ms5632-18":"0.47","ms5632-19":"0.75","ms5632-20":"0.55","ms5632-21":"0.41","ms5632-22":"0.95","ms5632-23":"0.48","ms5632-24":"0.79","ms5632-25":"0.53","ms5632-26":"0.87","ms5632-27":"0.55","ms5632-28":"0.89","ms5632-29":"0.64","ms5632-30":"1.13","ms5632-31":"0.71","ms5632-32":"0.51","ms5632-33":"0.69","ms5632-34":"0.86","ms5632-35":"0.50","ms5632-36":"0.41"},{"Metabolite":"C18:1","ms5632-1":"0.04","ms5632-2":"0.02","ms5632-3":"0.02","ms5632-4":"0.02","ms5632-5":"0.03","ms5632-6":"0.04","ms5632-7":"0.05","ms5632-8":"0.06","ms5632-9":"0.06","ms5632-10":"0.05","ms5632-11":"0.07","ms5632-12":"0.08","ms5632-13":"0.02","ms5632-14":"0.07","ms5632-15":"0.05","ms5632-16":"0.04","ms5632-17":"0.04","ms5632-18":"0.03","ms5632-19":"0.04","ms5632-20":"0.02","ms5632-21":"0.04","ms5632-22":"0.03","ms5632-23":"0.03","ms5632-24":"0.01","ms5632-25":"0.10","ms5632-26":"0.12","ms5632-27":"0.05","ms5632-28":"0.08","ms5632-29":"0.13","ms5632-30":"0.12","ms5632-31":"0.10","ms5632-32":"0.08","ms5632-33":"0.22","ms5632-34":"0.04","ms5632-35":"0.04","ms5632-36":"0.06"},{"Metabolite":"C18","ms5632-1":"7.93","ms5632-2":"5.63","ms5632-3":"4.37","ms5632-4":"8.82","ms5632-5":"5.59","ms5632-6":"4.99","ms5632-7":"2.51","ms5632-8":"4.82","ms5632-9":"5.10","ms5632-10":"2.38","ms5632-11":"5.07","ms5632-12":"4.31","ms5632-13":"5.47","ms5632-14":"6.69","ms5632-15":"6.45","ms5632-16":"4.17","ms5632-17":"5.88","ms5632-18":"4.73","ms5632-19":"6.53","ms5632-20":"6.29","ms5632-21":"5.11","ms5632-22":"6.78","ms5632-23":"4.96","ms5632-24":"5.07","ms5632-25":"9.18","ms5632-26":"15.17","ms5632-27":"12.97","ms5632-28":"8.20","ms5632-29":"8.96","ms5632-30":"19.83","ms5632-31":"9.54","ms5632-32":"13.31","ms5632-33":"12.53","ms5632-34":"7.76","ms5632-35":"9.19","ms5632-36":"6.48"},{"Metabolite":"C20","ms5632-1":"0.09","ms5632-2":"0.08","ms5632-3":"0.07","ms5632-4":"0.15","ms5632-5":"0.07","ms5632-6":"0.07","ms5632-7":"0.18","ms5632-8":"0.40","ms5632-9":"0.35","ms5632-10":"0.24","ms5632-11":"0.36","ms5632-12":"0.51","ms5632-13":"0.07","ms5632-14":"0.18","ms5632-15":"0.15","ms5632-16":"0.05","ms5632-17":"0.10","ms5632-18":"0.06","ms5632-19":"0.21","ms5632-20":"0.14","ms5632-21":"0.10","ms5632-22":"0.34","ms5632-23":"0.10","ms5632-24":"0.15","ms5632-25":"0.13","ms5632-26":"0.32","ms5632-27":"0.24","ms5632-28":"0.14","ms5632-29":"0.31","ms5632-30":"0.70","ms5632-31":"0.41","ms5632-32":"0.41","ms5632-33":"0.41","ms5632-34":"0.24","ms5632-35":"0.15","ms5632-36":"0.12"},{"Metabolite":"C22","ms5632-1":"0.20","ms5632-2":"0.18","ms5632-3":"0.15","ms5632-4":"0.30","ms5632-5":"0.16","ms5632-6":"0.14","ms5632-7":"0.27","ms5632-8":"0.43","ms5632-9":"0.39","ms5632-10":"0.35","ms5632-11":"0.60","ms5632-12":"0.55","ms5632-13":"0.16","ms5632-14":"0.29","ms5632-15":"0.30","ms5632-16":"0.14","ms5632-17":"0.21","ms5632-18":"0.15","ms5632-19":"0.25","ms5632-20":"0.19","ms5632-21":"0.16","ms5632-22":"0.31","ms5632-23":"0.16","ms5632-24":"0.22","ms5632-25":"0.33","ms5632-26":"0.52","ms5632-27":"0.41","ms5632-28":"0.28","ms5632-29":"0.38","ms5632-30":"0.83","ms5632-31":"0.44","ms5632-32":"0.41","ms5632-33":"0.50","ms5632-34":"0.32","ms5632-35":"0.27","ms5632-36":"0.23"},{"Metabolite":"C24:1","ms5632-1":"1.27","ms5632-2":"1.35","ms5632-3":"0.98","ms5632-4":"1.85","ms5632-5":"1.41","ms5632-6":"1.37","ms5632-7":"2.68","ms5632-8":"3.74","ms5632-9":"4.29","ms5632-10":"2.95","ms5632-11":"6.07","ms5632-12":"5.67","ms5632-13":"1.01","ms5632-14":"1.57","ms5632-15":"2.07","ms5632-16":"1.19","ms5632-17":"1.35","ms5632-18":"1.07","ms5632-19":"1.67","ms5632-20":"1.36","ms5632-21":"1.15","ms5632-22":"2.69","ms5632-23":"1.21","ms5632-24":"1.49","ms5632-25":"1.32","ms5632-26":"2.32","ms5632-27":"1.58","ms5632-28":"1.45","ms5632-29":"1.63","ms5632-30":"3.36","ms5632-31":"2.12","ms5632-32":"1.84","ms5632-33":"2.56","ms5632-34":"1.45","ms5632-35":"1.31","ms5632-36":"0.92"},{"Metabolite":"C24","ms5632-1":"0.43","ms5632-2":"0.43","ms5632-3":"0.29","ms5632-4":"0.71","ms5632-5":"0.38","ms5632-6":"0.35","ms5632-7":"0.65","ms5632-8":"1.25","ms5632-9":"1.22","ms5632-10":"0.87","ms5632-11":"1.81","ms5632-12":"1.69","ms5632-13":"0.33","ms5632-14":"1.09","ms5632-15":"0.99","ms5632-16":"0.34","ms5632-17":"0.54","ms5632-18":"0.33","ms5632-19":"0.60","ms5632-20":"0.50","ms5632-21":"0.34","ms5632-22":"1.09","ms5632-23":"0.39","ms5632-24":"0.70","ms5632-25":"0.51","ms5632-26":"1.25","ms5632-27":"0.84","ms5632-28":"0.58","ms5632-29":"0.61","ms5632-30":"1.44","ms5632-31":"1.10","ms5632-32":"0.85","ms5632-33":"1.04","ms5632-34":"0.85","ms5632-35":"0.66","ms5632-36":"0.48"}],

"Metabolites":[{"metabolite_name":"Sph"},{"metabolite_name":"Spa"},{"metabolite_name":"S1P"},{"metabolite_name":"C8"},{"metabolite_name":"C14"},{"metabolite_name":"C16"},{"metabolite_name":"C18:1"},{"metabolite_name":"C18"},{"metabolite_name":"C20"},{"metabolite_name":"C22"},{"metabolite_name":"C24:1"},{"metabolite_name":"C24"}]
}

}