{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000548","ANALYSIS_ID":"AN000836","VERSION":"1","CREATED_ON":"February 8, 2017, 1:23 pm"},

"PROJECT":{"PROJECT_TITLE":"Reproducibility study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate","PROJECT_SUMMARY":"The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from “The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate” by Ward and colleagues, published in Cancer Cell in 2010 (Ward et al., 2010). The experiments that will be replicated are those reported in Figures 2, 3 and 5. Ward and colleagues demonstrate the mutations in isocitrate dehydrogenase 2 (IDH2), commonly found in acute myeloid leukemia (AML), abrogate the enzyme’s wild-type activity and confer to the mutant neomorphic activity that produces the oncometabolite 2-hydroxyglutarate (2-HG) (Figures 2 and 3). They then show that elevated levels of 2-HG are correlated with mutations in IDH1 and IDH2in AML patient samples (Figure 5). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published by eLife.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biolmedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"Health Sciences Drive, Davis, California, 95616, USA","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","FUNDING_SOURCE":"NIH U24DK097154"},

"STUDY":{"STUDY_TITLE":"Replication study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate","STUDY_SUMMARY":"The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (Errington et al., 2014). This Registered Report describes the proposed replication plan of key experiments from “The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate” by Ward and colleagues, published in Cancer Cell in 2010 (Ward et al., 2010). The experiments that will be replicated are those reported in Figures 2, 3 and 5. Ward and colleagues demonstrate the mutations in isocitrate dehydrogenase 2 (IDH2), commonly found in acute myeloid leukemia (AML), abrogate the enzyme’s wild-type activity and confer to the mutant neomorphic activity that produces the oncometabolite 2-hydroxyglutarate (2-HG) (Figures 2 and 3). They then show that elevated levels of 2-HG are correlated with mutations in IDH1 and IDH2in AML patient samples (Figure 5). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published by eLife.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"Health Sciences Drive, Davis, California, 95616, USA","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258"},

"SUBJECT":{"SUBJECT_TYPE":"Cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
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{
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Vector"}
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{
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"Sample ID":"MUT09",
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"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj11_CS17_MUT07_24.D",
"Sample ID":"MUT07",
"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj12_CS16_MUT06_24.D",
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
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{
"Subject ID":"Inj13_CS02_WT02_24.D",
"Sample ID":"WT02",
"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
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},
{
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"Sample ID":"WT07",
"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj15_CS05_WT05_24.D",
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
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{
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{
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{
"Subject ID":"Inj31_CS13_MUT03_24.D",
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{
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"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
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{
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
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{
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
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{
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"Factors":{"Time Point":"CS-24h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Vector"}
},
{
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"Factors":{"Time Point":"QC","Sample Source":"-"},
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},
{
"Subject ID":"Inj46_HR_11_IDH1.D",
"Sample ID":"HR_11_IDH1",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH1 Mutant (R132C)"}
},
{
"Subject ID":"Inj47_HR_09_IDH1.D",
"Sample ID":"HR_09_IDH1",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
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{
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"Sample ID":"HR_02_WT",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
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{
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"Sample ID":"HR_05_IDH2",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
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{
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"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
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},
{
"Subject ID":"Inj53_HR_08_IDH2.D",
"Sample ID":"HR_08_IDH2",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj54_HR_12_IDH1.D",
"Sample ID":"HR_12_IDH1",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH1 Mutant (R132C)"}
},
{
"Subject ID":"Inj55_HR_03_WT.D",
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"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH Wild Type"}
},
{
"Subject ID":"Inj56_HR_06_IDH2.D",
"Sample ID":"HR_06_IDH2",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj57_HR_04_WT.D",
"Sample ID":"HR_04_WT",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH Wild Type"}
},
{
"Subject ID":"Inj58_HR_01_WT.D",
"Sample ID":"HR_01_WT",
"Factors":{"Time Point":"HR","Sample Source":"Peripheral blood mono-nuclear cells"},
"Additional sample data":{"Sample Type":"IDH Wild Type"}
},
{
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},
{
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},
{
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},
{
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"Sample ID":"CS49_MUT09_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
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},
{
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"Sample ID":"CS43_MUT03_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj11_CS56_V06.D",
"Sample ID":"CS56_V06_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj12_CS53_V03.D",
"Sample ID":"CS53_V03_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj13_CS37_WT07.D",
"Sample ID":"CS37_WT07_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj14_CS47_MUT07.D",
"Sample ID":"CS47_MUT07_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj15_CS58_V08.D",
"Sample ID":"CS58_V08_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj16_CS51_V01.D",
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"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
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"Sample ID":"CS33_WT03_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj18_CS44_MUT04.D",
"Sample ID":"CS44_MUT04_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj19_STD6_liner3.D",
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"Factors":{"Time Point":"QC","Sample Source":"-"},
"Additional sample data":{"Sample Type":"Quality Control"}
},
{
"Subject ID":"Inj20_CS41_MUT01.D",
"Sample ID":"CS41_MUT01_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj21_CS39_WT09.D",
"Sample ID":"CS39_WT09_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj22_CS52_V02.D",
"Sample ID":"CS52_V02_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
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},
{
"Subject ID":"Inj23_CS48_MUT08.D",
"Sample ID":"CS48_MUT08_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj24_CS42_MUT02.D",
"Sample ID":"CS42_MUT02_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj25_CS57_V07.D",
"Sample ID":"CS57_V07_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj26_CS45_MUT05.D",
"Sample ID":"CS45_MUT05_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj27_CS34_WT04.D",
"Sample ID":"CS34_WT04_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj28_CS36_WT06.D",
"Sample ID":"CS36_WT06_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj29_CS50_MUT10.D",
"Sample ID":"CS50_MUT10_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj30_STD6_liner3.D",
"Sample ID":"QC010",
"Factors":{"Time Point":"QC","Sample Source":"-"},
"Additional sample data":{"Sample Type":"Quality Control"}
},
{
"Subject ID":"Inj31_CS40_WT10.D",
"Sample ID":"CS40_WT10_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj32_CS38_WT08.D",
"Sample ID":"CS38_WT08_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj33_CS55_V05.D",
"Sample ID":"CS55_V05_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj34_CS59_V09.D",
"Sample ID":"CS59_V09_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj35_CS54_V04.D",
"Sample ID":"CS54_V04_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj36_CS35_WT05.D",
"Sample ID":"CS35_WT05_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj37_CS46_MUT06.D",
"Sample ID":"CS46_MUT06_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Mutant (R172K)"}
},
{
"Subject ID":"Inj38_CS32_WT02.D",
"Sample ID":"CS32_WT02_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj39_CS60_V10.D",
"Sample ID":"CS60_V10_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"Empty Vector Control"}
},
{
"Subject ID":"Inj40_CS31_WT01.D",
"Sample ID":"CS31_WT01_48",
"Factors":{"Time Point":"CS-48h","Sample Source":"293T HEK cell line"},
"Additional sample data":{"Sample Type":"IDH2 Wild Type"}
},
{
"Subject ID":"Inj41_STD6_liner3.D",
"Sample ID":"QC011",
"Factors":{"Time Point":"QC","Sample Source":"-"},
"Additional sample data":{"Sample Type":"Quality Control"}
},
{
"Subject ID":"Inj44_MB_liner3.D",
"Sample ID":"Blank003",
"Factors":{"Time Point":"Blank","Sample Source":"-"},
"Additional sample data":{"Sample Type":"MTBSTFA/ACN (1:1,v/v)"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"293T Cells were harvested 24 and 48 hours after transfection by removal of media and rapid quenching with 1.5 mL per 10 cm plate of -80°C methanol. Cells were incubated at -80°C for 15 minutesmin, then scraped off the dish and centrifuged for 5 min at 2,000 x g at 4°C to pellet cellular debris. Pellet was re-extracted by addition of 500 µL of -80°C 80% methanol in water, vortexed, then incubated at 4°C for 15 minutes and centrifuged for 5 minutes at 2,000 x g at 4°C. Peripheral blood mono-nuclear cells were centrifuged at 2,000 x g, freeze media removed and extracted using same procedure as 293T cells.","COLLECTION_PROTOCOL_FILENAME":"The common feature of IDH1 and IDH2 mutations.pdf","SAMPLE_TYPE":"Cells","COLLECTION_LOCATION":"UC Davis Genome and Biomedical Sciences Facility"},

"TREATMENT":{"TREATMENT_SUMMARY":"239T cells (ATCC CRL-3216) were grown in DMEM (Invitrogen) with 10% FBS (Hyclone) at -37°C in 10% CO2 and transfected with pcDNA3.1, pcDNA3.1-IDH2WT, pcDNA3.1-IDH2R172K (Invitrogen and Origene) with Lipofectamine 2000 (Invitrogen) according to manufacturer instructions. 6x10^5 cells were seeded in 6 well plates for protocol 1 and 3.5x10^6 cells were seeded in 10 cm plates for protocol 2. Identity of all vectors was confirmed by sequencing and vector integrity with agarose gel electrophoresis.","TREATMENT_PROTOCOL_FILENAME":"The common feature of IDH1 and IDH2 mutations.pdf","CELL_ENVIR_COND":"239T cells (ATCC CRL-3216) were grown in DMEM (Invitrogen) with 10% FBS (Hyclone) at 37°C in 10% CO2 and transfected with pcDNA3.1, pcDNA3.1-IDH2WT, pcDNA3.1-IDH2R172K (Invitrogen and Origene) with Lipofectamine 2000 (Invitrogen) according to manufacturer instructions.","CELL_HARVESTING":"24-48 hours after transfection"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Supernatants were combined and evaporated to dryness. Samples were then re-suspended in 200 µL LC-MS-grade water. A 2 mL AG-1 X8 100-200 anion exchange resin (Bio-Rad) was washed with 5 column volumes (10 mL) of 3 N HCl followed by transfer of re-suspended extracts to resin column. Metabolites were eluted using 10 mL 3 N HCl. Samples were evaporated to dryness and re-suspended in 100 µL MTBSTFA/ACN (1:1, v/v) and shaken at 60°C for 1 hour. Derivatized extracts were further diluted (1:4) with a MTBSTFA/ACN (1:1, v/v) mixture and transferred to glass vials with micro-inserts and capped immediately.","SAMPLEPREP_PROTOCOL_FILENAME":"The common feature of IDH1 and IDH2 mutations.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 7890A","COLUMN_NAME":"Agilent HP5-MS (30m × 0.25mm, 0.25 um)","FLOW_RATE":"1mL/min","COLUMN_TEMPERATURE":"250°C","METHODS_FILENAME":"Data_Dictionary_Fiehn_laboratory_GCTOF_MS_primary_metabolism_10-15-2013_general.pdf","SAMPLE_INJECTION":"0.2µl","OVEN_TEMPERATURE":"100°C (3 min), 4°C/min to 230°C (hold 4 min), 30°C/min to 300°C (hold 5 min)","TRANSFERLINE_TEMPERATURE":"230°C","RANDOMIZATION_ORDER":"Excel generated"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"WCMC Metabolomics Center","DETECTOR_TYPE":"TOF","SOFTWARE_VERSION":"Chromatof","DATA_FORMAT":".d"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","ION_SOURCE_TEMPERATURE":"230°C","IONIZATION_ENERGY":"70eV","MASS_ACCURACY":"Nominal","SCAN_RANGE_MOVERZ":"50-600 Da","SCANNING_CYCLE":"2.71 scans/sec","SCANNING_RANGE":"50-600 Da"},

"MS_METABOLITE_DATA":{
"Units":"Counts",

"Data":[{"Metabolite":"Asp TIC Results","V09":"62067.0586","MUT09":"68997.33993","MUT07":"75371.38813","MUT06":"70801.55076","WT02":"57925.81691","WT07":"54193.40238","WT05":"70498.59874","MUT04":"75943.91992","V07":"64545.09647","MUT08":"85047.2726","QC002":"447866.2448","WT04":"48154.70257","WT06":"57527.10983","V02":"195115.1489","WT03":"109874.8587","MUT10":"24718.9052","WT10":"42896.08221","WT01":"65546.02794","MUT05":"66255.50981","WT08":"52567.21863","V05":"59880.8502","QC003":"517862.6181","MUT03":"184595.3644","WT09":"68731.63646","V10":"69226.80163","V06":"69381.23022","V04":"50766.08415","V01":"66367.8631","MUT02":"89576.99165","V03":"75550.29143","MUT01":"82785.95803","V08":"54247.98495","QC004":"429798.3593","Blank001":"5306.82436","QC005":"508958.2916","HR_11_IDH1":"609124.4604","HR_09_IDH1":"199059.426","HR_02_WT":"498470.7185","HR_05_IDH2":"114465.8525","HR_07_IDH2":"294486.5884","HR_10_IDH1":"148462.0829","QC06":"830592.7041","HR_08_IDH2":"1251014.126","HR_12_IDH1":"30251.68959","HR_03_WT":"135569.8079","HR_06_IDH2":"493269.6016","HR_04_WT":"3450604.433","HR_01_WT":"207144.2845","Blank002":"10665.68855","QC007":"788290.8294","QC008":"620218.6602","CS49_MUT09_48":"22127.07494","CS43_MUT03_48":"55695.28688","CS56_V06_48":"27501.0992","CS53_V03_48":"48423.55338","CS37_WT07_48":"21037.22373","CS47_MUT07_48":"22634.30231","CS58_V08_48":"24947.40435","CS51_V01_48":"24803.51163","CS33_WT03_48":"33477.10421","CS44_MUT04_48":"15504.29583","QC009":"586575.8508","CS41_MUT01_48":"23392.70818","CS39_WT09_48":"22287.02709","CS52_V02_48":"22427.61428","CS48_MUT08_48":"22521.52089","CS42_MUT02_48":"31661.43607","CS57_V07_48":"30064.90361","CS45_MUT05_48":"20797.25454","CS34_WT04_48":"20106.64763","CS36_WT06_48":"23075.75919","CS50_MUT10_48":"20365.98566","QC010":"513001.8396","CS40_WT10_48":"20638.83746","CS38_WT08_48":"23649.2834","CS55_V05_48":"29344.76887","CS59_V09_48":"26826.7331","CS54_V04_48":"23241.50115","CS35_WT05_48":"24558.9413","CS46_MUT06_48":"18890.38544","CS32_WT02_48":"31039.5341","CS60_V10_48":"22522.23797","CS31_WT01_48":"19136.39006","QC011":"498748.7566","Blank003":"14472.85897"},{"Metabolite":"Asp EIC 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TIC Results","V09":"2379.6125","MUT09":"929937.0141","MUT07":"998440.9202","MUT06":"1069168.264","WT02":"1766.103125","WT07":"1957.321316","WT05":"2171.235413","MUT04":"1099299.67","V07":"1892.113359","MUT08":"1145497.368","QC002":"1622118.682","WT04":"1975.7625","WT06":"1905.813369","V02":"1984.771547","WT03":"1546.773382","MUT10":"345366.8361","WT10":"1970.146877","WT01":"1600.292662","MUT05":"1295079.348","WT08":"1979.033494","V05":"1900.014468","QC003":"2014774.842","MUT03":"1280467.562","WT09":"3027.920939","V10":"2127.9625","V06":"2185.223102","V04":"1412.21632","V01":"1225.53659","MUT02":"793902.9575","V03":"2112.97766","MUT01":"1011826.018","V08":"1652.465949","QC004":"1557615.714","Blank001":"5827.246875","QC005":"2067240.808","HR_11_IDH1":"170291.5777","HR_09_IDH1":"3583177.525","HR_02_WT":"5628.459375","HR_05_IDH2":"110893.0043","HR_07_IDH2":"988477.7222","HR_10_IDH1":"62002.62615","QC06":"2617796.438","HR_08_IDH2":"3029049.197","HR_12_IDH1":"77559.86208","HR_03_WT":"7178.801639","HR_06_IDH2":"1450304.234","HR_04_WT":"11398.43176","HR_01_WT":"4855.188278","Blank002":"5736.79375","QC007":"2501630.235","QC008":"2308320.5","CS49_MUT09_48":"677076.7539","CS43_MUT03_48":"157407.1728","CS56_V06_48":"970.9440275","CS53_V03_48":"1329.561653","CS37_WT07_48":"1737.817176","CS47_MUT07_48":"767532.3147","CS58_V08_48":"3001.959482","CS51_V01_48":"1516.810698","CS33_WT03_48":"1431.522293","CS44_MUT04_48":"340394.3165","QC009":"2295711.772","CS41_MUT01_48":"410910.7765","CS39_WT09_48":"1573.514574","CS52_V02_48":"1086.680241","CS48_MUT08_48":"628545.7785","CS42_MUT02_48":"197697.5771","CS57_V07_48":"1267.5","CS45_MUT05_48":"352363.9151","CS34_WT04_48":"1232.087565","CS36_WT06_48":"1126.125","CS50_MUT10_48":"387689.2244","QC010":"2118710.26","CS40_WT10_48":"1059.402263","CS38_WT08_48":"939.9966076","CS55_V05_48":"1131.44375","CS59_V09_48":"1327.84375","CS54_V04_48":"1295.81875","CS35_WT05_48":"1672.782695","CS46_MUT06_48":"714603.4415","CS32_WT02_48":"2195.4","CS60_V10_48":"1634.450019","CS31_WT01_48":"2509.975414","QC011":"2056238.225","Blank003":"4077.5625"},{"Metabolite":"2HG EIC 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TIC 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"Metabolites":[{"metabolite_name":"Asp TIC Results"},{"metabolite_name":"Asp EIC Results"},{"metabolite_name":"2HG TIC Results"},{"metabolite_name":"2HG EIC Results"},{"metabolite_name":"Glu TIC Results"},{"metabolite_name":"Glu EIC Results"},{"metabolite_name":"Ratio of 2HG/Glu TIC"},{"metabolite_name":"Ratio of 2HG/Glu EIC"}]
}

}