{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000549","ANALYSIS_ID":"AN000837","VERSION":"1","CREATED_ON":"February 9, 2017, 9:21 am"},
"PROJECT":{"PROJECT_TITLE":"Mayo Metabolomics Pilot and Feasibility Award: Role of muscle insulin and IGF-1 signaling on serum and muscle metabolite profiles","PROJECT_SUMMARY":"Skeletal muscle insulin resistance is a cardinal feature of the pathogenesis of type 2 diabetes. Insulin and IGF-1 signal through their highly related receptors to impact on many aspects of muscle physiology including glucose homeostasis, protein metabolism, and mitochondrial function. Early physiological studies, as well as recent large scale metabolomic studies, have shown that changes in specific pools of circulating amino acid metabolites, such as branched chain amino acids (BCAAs), are associated with insulin resistance and can predict future diabetes, but the source and impact of these changes in amino acids are not fully understood. We have recently generated mice which lack insulin receptors (IR) or IGF-1 receptors (IGF1R) or both in muscle using Cre lox recombination. We find that mice which lack only IR or only IGF1R in muscle show minimal changes in muscle mass, but do display increases in proteasomal activity and autophagy in muscle. On the other hand, mice with combined loss of both IR and IGF1R display markedly decreased muscle mass and enhanced degradation pathways, associated with increased protein synthesis, and display changes in mitochondrial gene regulation, indicating that both receptors can compensate to some extent for loss of the other. We hypothesize that IR and IGF1R signaling in muscle coordinate amino acid metabolite turnover and fuel substrate/mitochondrial metabolism, and that in insulin resistant states, changes in protein metabolism and mitochondrial function disrupt relative proportions of amino acid metabolites, which in turn contribute to diabetes risk and/or muscle pathology. We propose to test this hypothesis by performing large scale metabolomics on serum and muscle from mice lacking IR, IGF1R or both in muscle, and we will compare these changes to both insulin deficient streptozotocin-treated and insulin resistant diet-induced obese mouse models. To gain insight into which pathways are critical for metabolite changes, we will also treat mice with specific inhibitors of mTOR, a common protein synthesis pathway, as well as inhibitors of autophagy or proteasomal degradation and determine metabolite concentrations in muscle and serum. These studies will identify specific pathways that impact amino acid and mitochondrial metabolite flux which are perturbed in insulin resistant states, and potentially provide insights into how changes in amino acid metabolites contribute to diabetes risk.","INSTITUTE":"Mayo Clinic","LAST_NAME":"O'Neill","FIRST_NAME":"Brian","ADDRESS":"One Joslin Place, Boston, MA 02215","EMAIL":"brian.o'neill@joslin.harvard.edu","PHONE":"617-309-2400"},
"STUDY":{"STUDY_TITLE":"Investigating large scale metabolomics in mice serum lacking insulin receptors and IGF-1 receptors","STUDY_SUMMARY":"Large scale metabolomics from control, M-IR-/-, M-IGF1R-/- , and MIGIRKO mice serum. Also compare mice on a chow diet to mice on a high fat diet (HFD).","INSTITUTE":"Mayo Clinic","LAST_NAME":"O'Neill","FIRST_NAME":"Brian","ADDRESS":"One Joslin Place, Boston, MA 02215","EMAIL":"brian.o'neill@joslin.harvard.edu","PHONE":"617-309-2400"},
"SUBJECT":{"SUBJECT_TYPE":"Mouse","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10900"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"867",
"Sample ID":"19jan15_44",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"NHILIC"}
},
{
"Subject ID":"740",
"Sample ID":"19jan15_45",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1191",
"Sample ID":"19jan15_46",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1227",
"Sample ID":"19jan15_47",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1192",
"Sample ID":"19jan15_48",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"783",
"Sample ID":"19jan15_49",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"869",
"Sample ID":"19jan15_50",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"NHILIC"}
},
{
"Subject ID":"865",
"Sample ID":"19jan15_51",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"NHILIC"}
},
{
"Subject ID":"780",
"Sample ID":"19jan15_52",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"743",
"Sample ID":"19jan15_53",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"741",
"Sample ID":"19jan15_54",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"189",
"Sample ID":"19jan15_55",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1236",
"Sample ID":"19jan15_56",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"817",
"Sample ID":"19jan15_57",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"546",
"Sample ID":"19jan15_58",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"723",
"Sample ID":"19jan15_59",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"721",
"Sample ID":"19jan15_60",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"813",
"Sample ID":"19jan15_61",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"814",
"Sample ID":"19jan15_62",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"742",
"Sample ID":"19jan15_63",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"193",
"Sample ID":"19jan15_64",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1195",
"Sample ID":"19jan15_65",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"722",
"Sample ID":"19jan15_66",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1226",
"Sample ID":"19jan15_67",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"719",
"Sample ID":"19jan15_68",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1228",
"Sample ID":"19jan15_69",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"185",
"Sample ID":"19jan15_70",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"747",
"Sample ID":"19jan15_71",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"191",
"Sample ID":"19jan15_72",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1238",
"Sample ID":"19jan15_73",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"781",
"Sample ID":"19jan15_74",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1193",
"Sample ID":"19jan15_75",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"1221",
"Sample ID":"19jan15_76",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"549",
"Sample ID":"19jan15_77",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"850",
"Sample ID":"19jan15_78",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"NHILIC"}
},
{
"Subject ID":"864",
"Sample ID":"19jan15_79",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"NHILIC"}
},
{
"Subject ID":"551",
"Sample ID":"19jan15_80",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"NHILIC"}
},
{
"Subject ID":"867",
"Sample ID":"19jan15_04-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PHILIC"}
},
{
"Subject ID":"740",
"Sample ID":"19jan15_05",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1191",
"Sample ID":"19jan15_06",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1227",
"Sample ID":"19jan15_07",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1192",
"Sample ID":"19jan15_08",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"783",
"Sample ID":"19jan15_09",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"869",
"Sample ID":"19jan15_10",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PHILIC"}
},
{
"Subject ID":"865",
"Sample ID":"19jan15_11",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PHILIC"}
},
{
"Subject ID":"780",
"Sample ID":"19jan15_12",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"743",
"Sample ID":"19jan15_13",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"741",
"Sample ID":"19jan15_14",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"189",
"Sample ID":"19jan15_15",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1236",
"Sample ID":"19jan15_16",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"817",
"Sample ID":"19jan15_17",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"546",
"Sample ID":"19jan15_18",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"723",
"Sample ID":"19jan15_19",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"721",
"Sample ID":"19jan15_20",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"813",
"Sample ID":"19jan15_21",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"814",
"Sample ID":"19jan15_22",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"742",
"Sample ID":"19jan15_23",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"193",
"Sample ID":"19jan15_24",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1195",
"Sample ID":"19jan15_25",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"722",
"Sample ID":"19jan15_26",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1226",
"Sample ID":"19jan15_27",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"719",
"Sample ID":"19jan15_28",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1228",
"Sample ID":"19jan15_29",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"185",
"Sample ID":"19jan15_30",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"747",
"Sample ID":"19jan15_31",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"191",
"Sample ID":"19jan15_32",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1238",
"Sample ID":"19jan15_33",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"781",
"Sample ID":"19jan15_34",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1193",
"Sample ID":"19jan15_35",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"1221",
"Sample ID":"19jan15_36",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"549",
"Sample ID":"19jan15_37",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"850",
"Sample ID":"19jan15_38",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PHILIC"}
},
{
"Subject ID":"864",
"Sample ID":"19jan15_39",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PHILIC"}
},
{
"Subject ID":"551",
"Sample ID":"19jan15_40",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PHILIC"}
},
{
"Subject ID":"867",
"Sample ID":"05jan15_04-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"867",
"Sample ID":"05jan15_04-r002",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"740",
"Sample ID":"05jan15_05-r001",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"740",
"Sample ID":"05jan15_05-r002",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1191",
"Sample ID":"05jan15_06-r001",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1191",
"Sample ID":"05jan15_06-r002",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1227",
"Sample ID":"05jan15_07-r001",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1227",
"Sample ID":"05jan15_07-r002",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1192",
"Sample ID":"05jan15_08-r001",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1192",
"Sample ID":"05jan15_08-r002",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"783",
"Sample ID":"05jan15_09-r001",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"783",
"Sample ID":"05jan15_09-r002",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"869",
"Sample ID":"05jan15_10-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"869",
"Sample ID":"05jan15_10-r002",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"865",
"Sample ID":"05jan15_11-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"865",
"Sample ID":"05jan15_11-r002",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"780",
"Sample ID":"05jan15_12-r001",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"780",
"Sample ID":"05jan15_12-r002",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"743",
"Sample ID":"05jan15_13-r001",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"743",
"Sample ID":"05jan15_13-r002",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"741",
"Sample ID":"05jan15_14-r001",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"741",
"Sample ID":"05jan15_14-r002",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"189",
"Sample ID":"05jan15_15-r001",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"189",
"Sample ID":"05jan15_15-r002",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1236",
"Sample ID":"05jan15_16-r001",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1236",
"Sample ID":"05jan15_16-r002",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"817",
"Sample ID":"05jan15_17-r001",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"817",
"Sample ID":"05jan15_17-r002",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"546",
"Sample ID":"05jan15_18-r001",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"546",
"Sample ID":"05jan15_18-r002",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"723",
"Sample ID":"05jan15_19-r001",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"723",
"Sample ID":"05jan15_19-r002",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"721",
"Sample ID":"05jan15_20-r001",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"721",
"Sample ID":"05jan15_20-r002",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"813",
"Sample ID":"05jan15_21-r001",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"813",
"Sample ID":"05jan15_21-r002",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"814",
"Sample ID":"05jan15_22-r001",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"814",
"Sample ID":"05jan15_22-r002",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"742",
"Sample ID":"05jan15_23-r001",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"742",
"Sample ID":"05jan15_23-r002",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"193",
"Sample ID":"05jan15_24-r001",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"193",
"Sample ID":"05jan15_24-r002",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1195",
"Sample ID":"05jan15_25-r001",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1195",
"Sample ID":"05jan15_25-r002",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"722",
"Sample ID":"05jan15_26-r001",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"722",
"Sample ID":"05jan15_26-r002",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1226",
"Sample ID":"05jan15_27-r001",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1226",
"Sample ID":"05jan15_27-r002",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"719",
"Sample ID":"05jan15_28-r001",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"719",
"Sample ID":"05jan15_28-r002",
"Factors":{"Group":"Control","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1228",
"Sample ID":"05jan15_29-r001",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1228",
"Sample ID":"05jan15_29-r002",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"185",
"Sample ID":"05jan15_30-r001",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"185",
"Sample ID":"05jan15_30-r002",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"747",
"Sample ID":"05jan15_31-r001",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"747",
"Sample ID":"05jan15_31-r002",
"Factors":{"Group":"Control","Genotype":"IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"191",
"Sample ID":"05jan15_32-r001",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"191",
"Sample ID":"05jan15_32-r002",
"Factors":{"Group":"Control","Genotype":"IRlox IGFR lox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1238",
"Sample ID":"05jan15_33-r001",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1238",
"Sample ID":"05jan15_33-r002",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"781",
"Sample ID":"05jan15_34-r001",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"781",
"Sample ID":"05jan15_34-r002",
"Factors":{"Group":"IGFRKO","Genotype":"IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1193",
"Sample ID":"05jan15_35-r001",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1193",
"Sample ID":"05jan15_35-r002",
"Factors":{"Group":"CD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1221",
"Sample ID":"05jan15_36-r001",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"1221",
"Sample ID":"05jan15_36-r002",
"Factors":{"Group":"MIGIRKO","Genotype":"IR -/- IGFR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"549",
"Sample ID":"05jan15_37-r001",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"1","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"549",
"Sample ID":"05jan15_37-r002",
"Factors":{"Group":"IRKO","Genotype":"IR -/-"},
"Additional sample data":{"replicate":"2","Diet":"Chow","chromatography":"PC18"}
},
{
"Subject ID":"850",
"Sample ID":"05jan15_38-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"850",
"Sample ID":"05jan15_38-r002",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"864",
"Sample ID":"05jan15_39-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"864",
"Sample ID":"05jan15_39-r002",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"-",
"Sample ID":"05jan15_40-r001",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"1","Diet":"High Fat Diet","chromatography":"PC18"}
},
{
"Subject ID":"-",
"Sample ID":"05jan15_40-r002",
"Factors":{"Group":"HFD","Genotype":"Irlox"},
"Additional sample data":{"replicate":"2","Diet":"High Fat Diet","chromatography":"PC18"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"mouse serum"},
"TREATMENT":{"TREATMENT_SUMMARY":"Mice lacking insulin receptors (IR -/- genotype, IRKO group), or IGF-1 receptors (ICF-1 -/- genotype, IGFRKO group), or both (MIGIRKO group) were generated using Cre lox recombination. Controls (group control and CD) were IR lox/lox, IGF-1 lox/lox, or both genotypes. Additional, 10 mice were included that were fed different diets for 8 weeks, chow or high fat diet (group HFD)."},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"large scale metabolomics in mouse serum"},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent 1290 Infinity","COLUMN_NAME":"Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS"},
"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6550 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},
"MS_METABOLITE_DATA":{
"Units":"intensity",
"Data":[{"Metabolite":"C18 H10 N6 O10 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