{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000623","ANALYSIS_ID":"AN001820","VERSION":"1","CREATED_ON":"January 10, 2019, 8:40 am"},

"PROJECT":{"PROJECT_TITLE":"Non-targeted Metabolomics Analysis of Golden Retriever Muscular Dystrophy-Affected Muscles Dog","PROJECT_TYPE":"GC-MS non targeted analysis","PROJECT_SUMMARY":"Non-targeted Metabolomics Analysis of Golden Retriever Muscular Dystrophy-Affected Muscles Dog","INSTITUTE":"University of North Carolina","DEPARTMENT":"McAllister heart Institute, Department of Internal medicine","LABORATORY":"Multiple Centers","LAST_NAME":"Willis","FIRST_NAME":"Monte","ADDRESS":"111 Mason Farm road, Chapel Hill, North Carolina, 27599-7126, USA","EMAIL":"monte_willis@med.unc.edu","PHONE":"(984) 999-5431","FUNDING_SOURCE":"NIH, Fondation Leducq"},

"STUDY":{"STUDY_TITLE":"Non-targeted Metabolomics Analysis of Golden Retriever Muscular Dystrophy-Affected Muscles Reveals Alterations in Arginine and Proline Metabolism, and Elevations in Glutamic and Oleic Acid In Vivo","STUDY_SUMMARY":"Like Duchenne muscular dystrophy (DMD), the Golden Retriever Muscular Dystrophy (GRMD) dog model of DMD exhibits is characterized by muscle necrosis, progressive paralysis, and pseudohypertrophy in specific skeletal muscles. This severe GRMD phenotype included atrophy of the biceps femoris (BF) and compared to unaffected normal dogs, while the long digital extensor (LDE) of the pelvic limb, serving as a hip flex and stifle extensor, is unaffected. A recent microarray analysis of GRMD identified alterations in genes associated with lipid metabolism and energy production. We, therefore, undertook a non-targeted metabolomics analysis of the GRMD BF (affected) and LDE (unaffected) using GC-MS to identify underlying metabolic defects specific for affected GRMD skeletal muscle. Of the 134 metabolites identified in BF, eight were significantly altered in GRMD BF compared to control BF (Glutamic Acid (2.48 fold vs. controls); Oleic Acid (1.76 fold vs. controls); Proline (1.73 fold vs. controls); Myoinositol-2- Phosphate (0.44 fold vs. controls); Fumaric Acid (0.40 fold vs. controls); Carnosine (0.40 fold vs. controls); Lactamide (0.33 fold vs. controls); and Stearamide (0.23 fold vs. controls). Pathway analysis of the T-test significant metabolites identified BF muscle metabolites significantly enriched for Arginine and proline metabolism (p=5.8E-4, FDR=0.04) and Alanine, aspartate, and glutamate metabolism (p=1.3E-3, FDR=0.05). The GRMD LDE previously reported to be unaffected, in contrast, had only one significantly altered metabolite (3-Phosphoglyceric Acid (0.35 Fold vs. controls)).The identification of elevated BF Oleic acid (a long-chain fatty acid) is consistent with recent microarray studies identifying altered lipid metabolism genes, while alterations in Arginine and Proline metabolism are consistent with recent studies identifying elevated L-arginine in DMD patient sera as a biomarker of disease (alterations in DMD or GRMD muscle itself have not previously been reported).Together, these studies demonstrate muscle-specific alterations in GRMD-affected muscle, which illustrate previously unidentified metabolic changes.","INSTITUTE":"University of North Carolina","DEPARTMENT":"McAllister heart Institute, Department of Internal medicine, Department of Pathology & Laboratory, Department of Pharmacology","LABORATORY":"Multiple Centers","LAST_NAME":"Willis","FIRST_NAME":"Monte","ADDRESS":"111 Mason Farm Road, MBRB 2336, Chapel Hill, North Carolina, 27599-7126, USA","EMAIL":"monte_willis@med.unc.edu","PHONE":"(984) 999-5431","STUDY_COMMENTS":"Skeletal Muscle (Biceps femoris (BF), long digital extensor(LDE))"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Canis lupus familiaris","TAXONOMY_ID":"9615"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"K 8",
"Factors":{"Gentotype":"Control BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 10",
"Factors":{"Gentotype":"Control BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 7",
"Factors":{"Gentotype":"Control BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 9",
"Factors":{"Gentotype":"Control BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 2",
"Factors":{"Gentotype":"GRMD BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 3",
"Factors":{"Gentotype":"GRMD BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 4",
"Factors":{"Gentotype":"GRMD BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 5",
"Factors":{"Gentotype":"GRMD BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 6",
"Factors":{"Gentotype":"GRMD BF"}
},
{
"Subject ID":"-",
"Sample ID":"K 1",
"Factors":{"Gentotype":"GRMD BF"}
},
{
"Subject ID":"-",
"Sample ID":"K17",
"Factors":{"Gentotype":"Control LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 19",
"Factors":{"Gentotype":"Control LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 18",
"Factors":{"Gentotype":"Control LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 20",
"Factors":{"Gentotype":"Control LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 12",
"Factors":{"Gentotype":"GRMD LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 13",
"Factors":{"Gentotype":"GRMD LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 14",
"Factors":{"Gentotype":"GRMD LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 16",
"Factors":{"Gentotype":"GRMD LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 11",
"Factors":{"Gentotype":"GRMD LDE"}
},
{
"Subject ID":"-",
"Sample ID":"K 15",
"Factors":{"Gentotype":"GRMD LDE"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Skeletal muscle (BF, LDE) was surgically biosied and flash frozen in liquid nitrogen.","SAMPLE_TYPE":"Muscle"},

"TREATMENT":{"TREATMENT_SUMMARY":"Fraction of cardiac tissue weighed (25-50 mg wet weight), then the finely cut up tissue quickly added to fresh pre-made buffer (50 % acetyl-nitrile, 50% water, 0.3% formic acid) at a standard concentration of 25 mg/475 mcl buffer then fully homogenized on ice for 20-25 s and placed on dry ice/stored at - 80C"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"The samples were crash deprotonized by methanol precipitation and spiked with D27-deuterated myristic acid (D27-C14:0) as an internal standard for retention-time locking and dried. The trimethylsilyl-D27-C14:0 standard retention time (RT) was set at 16.727 min. Reactive carbonyls were stabilized at 50C with methoxyamine hydrochloride in dry pyridine. Metabolites were made volatile with TMS groups using N-methyl-N-(trimethylsilyl) trifluoroacetamide or MSTFA with catalytic trimethylchlorosilane at 50C."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"GC/MS methods follow previous studies using a 6890 N GC connected to a 5975 Inert single quadrupole MS (Agilent Technologies, Santa Clara, CA) (Bonikos et al. 1975; Fiehn 2008; Kind et al. 2009). The two wall-coated, open-tubular GC columns connected in series are both from J&W/Agilent (part 122-5512), DB5-MS, 15 meters in length, 0.25 mm in diameter, with an 0.25-l m luminal film. Positive ions generated with conventional electron-ionization at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time.","CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent 6890N","COLUMN_NAME":"Agilent DB5-MS (15m × 0.25mm, 0.25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 5975 MS","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"none"},

"MS_METABOLITE_DATA":{
"Units":"Peak values (Log transformed)",

"Data":[{"Metabolite":"1,2-Propanediol","K 19":"18.36","K 18":"19.48","K 20":"18.62","K 12":"17.98","K 13":"18.24","K 14":"17.48","K 16":"18.46","K 11":"18.73","K 15":"18.82","K 8":"19.91","K 10":"14.2","K 7":"17.85","K 9":"20.51","K 2":"18.84","K 3":"18.67","K 4":"18.82","K 5":"16.53","K 6":"19.95","K 1":"16.52"},{"Metabolite":"1,5-Anhydroglucitol","K 19":"16.92","K 18":"18.17","K 20":"17.58","K 12":"15.83","K 13":"17.54","K 14":"18.11","K 16":"15.83","K 11":"","K 15":"16.63","K 8":"","K 10":"17.21","K 7":"17.94","K 9":"17.74","K 2":"18.16","K 3":"16.7","K 4":"17.34","K 5":"18.1","K 6":"18.03","K 1":"17.73"},{"Metabolite":"2-Aminoadipic Acid","K 19":"21.96","K 18":"20.93","K 20":"22.2","K 12":"21.8","K 13":"21.6","K 14":"21.5","K 16":"20.33","K 11":"19.56","K 15":"21.37","K 8":"22.02","K 10":"22.12","K 7":"21.78","K 9":"22.02","K 2":"22.44","K 3":"20.93","K 4":"21.62","K 5":"21.32","K 6":"21.63","K 1":"21.64"},{"Metabolite":"2-Hydroxybutyric Acid","K 19":"15.85","K 18":"14.4","K 20":"15.65","K 12":"14.23","K 13":"15.24","K 14":"16.2","K 16":"15.82","K 11":"","K 15":"14.76","K 8":"16.21","K 10":"","K 7":"","K 9":"15.61","K 2":"15.92","K 3":"16.28","K 4":"13.35","K 5":"","K 6":"16.15","K 1":"14.68"},{"Metabolite":"2-Hydroxyglutaric Acid","K 19":"18.06","K 18":"17.34","K 20":"18.05","K 12":"18.41","K 13":"17.4","K 14":"17.9","K 16":"17.07","K 11":"16.94","K 15":"17.66","K 8":"19.3","K 10":"17.61","K 7":"","K 9":"18.82","K 2":"","K 3":"18.33","K 4":"17.97","K 5":"","K 6":"18.29","K 1":"18.44"},{"Metabolite":"2-Hydroxypyridine","K 19":"17.4","K 18":"17.75","K 20":"17.45","K 12":"16.82","K 13":"17.45","K 14":"17.31","K 16":"17.23","K 11":"17.13","K 15":"17.4","K 8":"17.59","K 10":"17.43","K 7":"17.48","K 9":"17.51","K 2":"17.09","K 3":"17.61","K 4":"17.2","K 5":"17.54","K 6":"17.46","K 1":"17.93"},{"Metabolite":"3-Phosphoglyceric Acid","K 19":"17.58","K 18":"17.24","K 20":"17.34","K 12":"16.6","K 13":"16.67","K 14":"17.22","K 16":"","K 11":"14.62","K 15":"15.37","K 8":"15.08","K 10":"15.73","K 7":"14.64","K 9":"14.74","K 2":"","K 3":"13.69","K 4":"18.56","K 5":"17.28","K 6":"18.08","K 1":"15.62"},{"Metabolite":"4-Hydroxymandelic Acid","K 19":"14.47","K 18":"15.71","K 20":"15.06","K 12":"14.3","K 13":"14.45","K 14":"14.79","K 16":"15.34","K 11":"14.43","K 15":"14.89","K 8":"14.92","K 10":"15.21","K 7":"15.16","K 9":"14.97","K 2":"14.81","K 3":"15.26","K 4":"13.65","K 5":"","K 6":"14.74","K 1":"15.12"},{"Metabolite":"Adenine","K 19":"14.94","K 18":"","K 20":"14.88","K 12":"","K 13":"15.52","K 14":"15.45","K 16":"14.6","K 11":"15.78","K 15":"14.84","K 8":"16.38","K 10":"15.2","K 7":"15.11","K 9":"15.42","K 2":"15.29","K 3":"15.05","K 4":"15.55","K 5":"15.07","K 6":"15.31","K 1":"15.72"},{"Metabolite":"Adenosine","K 19":"17.66","K 18":"19.25","K 20":"18.35","K 12":"17.34","K 13":"17.53","K 14":"18.06","K 16":"18.88","K 11":"17.35","K 15":"17.96","K 8":"18.02","K 10":"18.77","K 7":"18.08","K 9":"18.84","K 2":"17.82","K 3":"18.61","K 4":"17.77","K 5":"16.02","K 6":"17.56","K 1":"17.87"},{"Metabolite":"Adenosine-5-Monophosphate","K 19":"20.35","K 18":"21.79","K 20":"20.43","K 12":"20.69","K 13":"18.58","K 14":"19.48","K 16":"20.1","K 11":"18.53","K 15":"21.6","K 8":"21.14","K 10":"18.55","K 7":"19","K 9":"22.02","K 2":"","K 3":"13.72","K 4":"19.21","K 5":"20.81","K 6":"20.38","K 1":"17.45"},{"Metabolite":"Alanine","K 19":"20.9","K 18":"21.07","K 20":"20.78","K 12":"20.26","K 13":"20.98","K 14":"20.76","K 16":"20.73","K 11":"20.01","K 15":"20.76","K 8":"21.13","K 10":"20.88","K 7":"21.04","K 9":"21.21","K 2":"21.25","K 3":"20.64","K 4":"20.87","K 5":"20.23","K 6":"20.64","K 1":"21.6"},{"Metabolite":"Alpha-Tocopherol","K 19":"16.9","K 18":"17.15","K 20":"16.62","K 12":"16.81","K 13":"16.52","K 14":"17.03","K 16":"16.49","K 11":"15.24","K 15":"16.25","K 8":"17.09","K 10":"15.85","K 7":"17.19","K 9":"16.14","K 2":"16.16","K 3":"16","K 4":"17.19","K 5":"16.76","K 6":"17.17","K 1":"16.46"},{"Metabolite":"Aminomalonic Acid","K 19":"17.97","K 18":"17.93","K 20":"17.01","K 12":"18.16","K 13":"17.86","K 14":"17.33","K 16":"17.61","K 11":"16.24","K 15":"17.04","K 8":"17.14","K 10":"17.4","K 7":"17.97","K 9":"17.36","K 2":"17.42","K 3":"17.3","K 4":"17.28","K 5":"17.55","K 6":"16.97","K 1":"17.95"},{"Metabolite":"Lactamide","K 19":"15.73","K 18":"15.34","K 20":"15.95","K 12":"","K 13":"15.48","K 14":"15.24","K 16":"","K 11":"14.24","K 15":"15.65","K 8":"13.98","K 10":"15.71","K 7":"14.26","K 9":"16.11","K 2":"","K 3":"11.95","K 4":"14.81","K 5":"12.61","K 6":"13.93","K 1":""},{"Metabolite":"Arachidonic Acid (Also Eicosapentaenoic Acid)","K 19":"19.18","K 18":"19.39","K 20":"19.76","K 12":"17.83","K 13":"19.52","K 14":"19.89","K 16":"18.93","K 11":"18.37","K 15":"19.43","K 8":"19.39","K 10":"19.56","K 7":"18.8","K 9":"19.43","K 2":"19.88","K 3":"20.63","K 4":"19.78","K 5":"16.82","K 6":"19.13","K 1":"20.52"},{"Metabolite":"Asparagine","K 19":"18.25","K 18":"18.25","K 20":"18.04","K 12":"18.83","K 13":"18.74","K 14":"18.39","K 16":"19.19","K 11":"17.86","K 15":"17.91","K 8":"19.18","K 10":"18.2","K 7":"19.15","K 9":"19.25","K 2":"18.77","K 3":"19.49","K 4":"18.3","K 5":"19.04","K 6":"18.62","K 1":"19.22"},{"Metabolite":"Aspartic Acid","K 19":"19.96","K 18":"20.07","K 20":"19.37","K 12":"19.48","K 13":"18.77","K 14":"20.14","K 16":"19.42","K 11":"18.19","K 15":"18.7","K 8":"20.06","K 10":"18.49","K 7":"20.57","K 9":"19.79","K 2":"19.55","K 3":"18.04","K 4":"18.97","K 5":"19.24","K 6":"19.71","K 1":"19.69"},{"Metabolite":"Beta-Alanine","K 19":"15.5","K 18":"16.52","K 20":"16.22","K 12":"20.13","K 13":"15.27","K 14":"15.12","K 16":"16.59","K 11":"16.4","K 15":"16.35","K 8":"15.84","K 10":"16.58","K 7":"15.69","K 9":"16.27","K 2":"15.36","K 3":"16.3","K 4":"14.36","K 5":"19.99","K 6":"14.88","K 1":"15.48"},{"Metabolite":"Beta-Hydroxybutyric Acid","K 19":"16.04","K 18":"16.48","K 20":"17.53","K 12":"16.69","K 13":"17.39","K 14":"16.46","K 16":"17.87","K 11":"16.06","K 15":"17.48","K 8":"16.58","K 10":"17.58","K 7":"15.4","K 9":"17.64","K 2":"16.54","K 3":"18.64","K 4":"17.05","K 5":"16.22","K 6":"16.28","K 1":"17.77"},{"Metabolite":"Campesterol","K 19":"15.05","K 18":"13.22","K 20":"14.4","K 12":"","K 13":"14.56","K 14":"13.61","K 16":"13.28","K 11":"","K 15":"13.91","K 8":"13.78","K 10":"13.9","K 7":"14.06","K 9":"14","K 2":"13.69","K 3":"","K 4":"14.7","K 5":"","K 6":"13.86","K 1":"11.53"},{"Metabolite":"Carnosine","K 19":"22.08","K 18":"23.04","K 20":"23.02","K 12":"22.16","K 13":"21.56","K 14":"21.44","K 16":"23.21","K 11":"23.12","K 15":"22.56","K 8":"22.77","K 10":"23.35","K 7":"22.14","K 9":"22.56","K 2":"20.57","K 3":"22.26","K 4":"21.28","K 5":"19.96","K 6":"21.41","K 1":"22.09"},{"Metabolite":"Cholesterol","K 19":"24.78","K 18":"24.35","K 20":"24.01","K 12":"24.69","K 13":"24.85","K 14":"24.94","K 16":"24.29","K 11":"22.85","K 15":"24","K 8":"24.96","K 10":"23.98","K 7":"24.16","K 9":"24.07","K 2":"24.53","K 3":"24.37","K 4":"24.42","K 5":"23.65","K 6":"24.86","K 1":"24.19"},{"Metabolite":"Citric Acid/Isocitric Acid","K 19":"19.96","K 18":"20.47","K 20":"20.07","K 12":"19.9","K 13":"20.11","K 14":"20.01","K 16":"19.18","K 11":"21.16","K 15":"19.95","K 8":"20.82","K 10":"19.2","K 7":"21.27","K 9":"21.16","K 2":"17.98","K 3":"17.47","K 4":"20.74","K 5":"19.99","K 6":"21.57","K 1":"20.18"},{"Metabolite":"Creatinine","K 19":"24.16","K 18":"24.89","K 20":"24.76","K 12":"24.9","K 13":"24.38","K 14":"24.24","K 16":"24.45","K 11":"24","K 15":"24.44","K 8":"24.23","K 10":"24.54","K 7":"23.96","K 9":"24.34","K 2":"24.42","K 3":"23.41","K 4":"24.25","K 5":"24.35","K 6":"23.66","K 1":"25.2"},{"Metabolite":"Dehydroascorbic Acid","K 19":"19.47","K 18":"17.16","K 20":"16.7","K 12":"18.59","K 13":"19.52","K 14":"19.66","K 16":"19.11","K 11":"16.73","K 15":"17.08","K 8":"18.8","K 10":"16.82","K 7":"16.24","K 9":"16.1","K 2":"18.56","K 3":"17.8","K 4":"19.11","K 5":"17.17","K 6":"18.67","K 1":"15.86"},{"Metabolite":"Disaccharide","K 19":"20.03","K 18":"19.11","K 20":"19.21","K 12":"15.83","K 13":"20.15","K 14":"19.83","K 16":"20.1","K 11":"20.34","K 15":"17.89","K 8":"18.47","K 10":"20.1","K 7":"16.44","K 9":"17.26","K 2":"16.68","K 3":"16.05","K 4":"19.24","K 5":"16.49","K 6":"18.13","K 1":"18.27"},{"Metabolite":"Ethanolamine","K 19":"16.49","K 18":"16.98","K 20":"17.29","K 12":"15.51","K 13":"16.9","K 14":"16.98","K 16":"16.38","K 11":"15.55","K 15":"16.5","K 8":"16.9","K 10":"17.15","K 7":"15.7","K 9":"16.49","K 2":"16.35","K 3":"17.71","K 4":"17.55","K 5":"15.31","K 6":"16.21","K 1":"16.45"},{"Metabolite":"Fructose Or Similar Ketohexose","K 19":"15.52","K 18":"13.73","K 20":"15.41","K 12":"14.2","K 13":"14.82","K 14":"14.8","K 16":"14.43","K 11":"13.79","K 15":"14.77","K 8":"13.31","K 10":"17.64","K 7":"12.12","K 9":"17.11","K 2":"","K 3":"","K 4":"13.36","K 5":"","K 6":"13.88","K 1":"16.07"},{"Metabolite":"Fructose-6-Phosphate","K 19":"20.65","K 18":"20.73","K 20":"19.9","K 12":"18.92","K 13":"19.27","K 14":"19.58","K 16":"19.64","K 11":"19.43","K 15":"21.48","K 8":"18.66","K 10":"22.01","K 7":"18.15","K 9":"20.6","K 2":"17.45","K 3":"18.11","K 4":"19.32","K 5":"17.09","K 6":"18.11","K 1":"22.77"},{"Metabolite":"Fumaric Acid","K 19":"18.76","K 18":"19.98","K 20":"19.66","K 12":"18.46","K 13":"18.47","K 14":"18.7","K 16":"19.61","K 11":"18.31","K 15":"19.52","K 8":"19.12","K 10":"19.27","K 7":"19.31","K 9":"20.16","K 2":"18.42","K 3":"18.34","K 4":"18.03","K 5":"17.74","K 6":"17.98","K 1":"18.55"},{"Metabolite":"Gamma-Hydroxybutyric Acid/Ethylmalonic Acid","K 19":"17.58","K 18":"15.6","K 20":"18.28","K 12":"15.39","K 13":"16.75","K 14":"17.25","K 16":"16.67","K 11":"15.49","K 15":"18","K 8":"15.32","K 10":"18.74","K 7":"13.59","K 9":"17.96","K 2":"15.6","K 3":"18.53","K 4":"16.78","K 5":"15.07","K 6":"14.66","K 1":"19.77"},{"Metabolite":"Glucose And Other Aldohexoses","K 19":"23.92","K 18":"23.38","K 20":"21.74","K 12":"21.38","K 13":"22.2","K 14":"23.67","K 16":"23.05","K 11":"23.13","K 15":"23.48","K 8":"21.64","K 10":"22.79","K 7":"19.89","K 9":"22.96","K 2":"22.5","K 3":"21.05","K 4":"22.61","K 5":"22.37","K 6":"23.44","K 1":"23.12"},{"Metabolite":"Glucose-6-Phosphate","K 19":"22.39","K 18":"22.12","K 20":"21.62","K 12":"20.51","K 13":"20.49","K 14":"21.29","K 16":"21.33","K 11":"20.67","K 15":"23.18","K 8":"20.09","K 10":"23.7","K 7":"19.61","K 9":"22.25","K 2":"18.55","K 3":"19.03","K 4":"20.57","K 5":"18.13","K 6":"19.75","K 1":"21"},{"Metabolite":"Glutamic Acid","K 19":"21.98","K 18":"21.97","K 20":"20.33","K 12":"21.07","K 13":"21.91","K 14":"22.05","K 16":"20.6","K 11":"19.57","K 15":"21.32","K 8":"20.86","K 10":"19.48","K 7":"21.71","K 9":"20.44","K 2":"21.77","K 3":"23.29","K 4":"22.11","K 5":"21.74","K 6":"21.62","K 1":"21.41"},{"Metabolite":"Glutamine","K 19":"22.91","K 18":"22.74","K 20":"22.74","K 12":"22.12","K 13":"22.73","K 14":"22.84","K 16":"22.46","K 11":"21.3","K 15":"22.02","K 8":"22.89","K 10":"21.82","K 7":"22.22","K 9":"22.59","K 2":"21.38","K 3":"23.53","K 4":"22.48","K 5":"21.52","K 6":"22.52","K 1":"21.61"},{"Metabolite":"Glycerol 1-Phosphate","K 19":"22.42","K 18":"23.42","K 20":"23.2","K 12":"21.89","K 13":"22.13","K 14":"21.91","K 16":"22.79","K 11":"21.37","K 15":"22.44","K 8":"20.75","K 10":"22.95","K 7":"20.45","K 9":"22.76","K 2":"20.53","K 3":"23.3","K 4":"22.62","K 5":"21.24","K 6":"19.84","K 1":"21.48"},{"Metabolite":"Glycine","K 19":"22.41","K 18":"22.07","K 20":"20.99","K 12":"22.66","K 13":"22.44","K 14":"21.98","K 16":"21.99","K 11":"20.93","K 15":"21.34","K 8":"23.15","K 10":"21.72","K 7":"22.5","K 9":"21.96","K 2":"22.08","K 3":"21.6","K 4":"22.1","K 5":"21.81","K 6":"21.86","K 1":"22.27"},{"Metabolite":"Hydroxyprolines","K 19":"17.16","K 18":"16.87","K 20":"15.56","K 12":"15.94","K 13":"18.01","K 14":"16.89","K 16":"15.77","K 11":"15.29","K 15":"16.71","K 8":"17.33","K 10":"16.9","K 7":"18.41","K 9":"17.86","K 2":"17.88","K 3":"17.23","K 4":"17.85","K 5":"14.59","K 6":"16.55","K 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"Metabolites":[{"metabolite_name":"1,2-Propanediol"},{"metabolite_name":"1,5-Anhydroglucitol"},{"metabolite_name":"2-Aminoadipic Acid"},{"metabolite_name":"2-Hydroxybutyric Acid"},{"metabolite_name":"2-Hydroxyglutaric Acid"},{"metabolite_name":"2-Hydroxypyridine"},{"metabolite_name":"3-Phosphoglyceric Acid"},{"metabolite_name":"4-Hydroxymandelic Acid"},{"metabolite_name":"Adenine"},{"metabolite_name":"Adenosine"},{"metabolite_name":"Adenosine-5-Monophosphate"},{"metabolite_name":"Alanine"},{"metabolite_name":"Alpha-Tocopherol"},{"metabolite_name":"Aminomalonic Acid"},{"metabolite_name":"Lactamide"},{"metabolite_name":"Arachidonic Acid (Also Eicosapentaenoic Acid)"},{"metabolite_name":"Asparagine"},{"metabolite_name":"Aspartic Acid"},{"metabolite_name":"Beta-Alanine"},{"metabolite_name":"Beta-Hydroxybutyric Acid"},{"metabolite_name":"Campesterol"},{"metabolite_name":"Carnosine"},{"metabolite_name":"Cholesterol"},{"metabolite_name":"Citric Acid/Isocitric Acid"},{"metabolite_name":"Creatinine"},{"metabolite_name":"Dehydroascorbic Acid"},{"metabolite_name":"Disaccharide"},{"metabolite_name":"Ethanolamine"},{"metabolite_name":"Fructose Or Similar Ketohexose"},{"metabolite_name":"Fructose-6-Phosphate"},{"metabolite_name":"Fumaric Acid"},{"metabolite_name":"Gamma-Hydroxybutyric Acid/Ethylmalonic Acid"},{"metabolite_name":"Glucose And Other Aldohexoses"},{"metabolite_name":"Glucose-6-Phosphate"},{"metabolite_name":"Glutamic Acid"},{"metabolite_name":"Glutamine"},{"metabolite_name":"Glycerol 1-Phosphate"},{"metabolite_name":"Glycine"},{"metabolite_name":"Hydroxyprolines"},{"metabolite_name":"Hypotaurine"},{"metabolite_name":"Hypoxanthine"},{"metabolite_name":"Inosine"},{"metabolite_name":"Isoleucine/Leucine"},{"metabolite_name":"Ketopentose-5-Phosphate"},{"metabolite_name":"Lactic Acid"},{"metabolite_name":"Linoleic Acid"},{"metabolite_name":"Lysine"},{"metabolite_name":"Malic Acid"},{"metabolite_name":"Maltose Or Similar Disaccharide"},{"metabolite_name":"Myoinositol"},{"metabolite_name":"Myoinositol-2-Phosphate"},{"metabolite_name":"N-Acetylneuraminic Acid"},{"metabolite_name":"N-Methylalanine/2-Aminobutanoic Acid/N-Ethylglycine"},{"metabolite_name":"O-Methylphosphate"},{"metabolite_name":"O-Phosphocolamine"},{"metabolite_name":"Oleic Acid"},{"metabolite_name":"Pantothenic Acid"},{"metabolite_name":"Phenylalanine"},{"metabolite_name":"Phosphoric Acid"},{"metabolite_name":"Proline"},{"metabolite_name":"Pyrophosphate"},{"metabolite_name":"Serine"},{"metabolite_name":"Siloxanes_R1"},{"metabolite_name":"Siloxanes_R2"},{"metabolite_name":"Siloxanes_R3"},{"metabolite_name":"Spermidine"},{"metabolite_name":"Spermine"},{"metabolite_name":"Squalene"},{"metabolite_name":"Stearamide"},{"metabolite_name":"Succinic Acid"},{"metabolite_name":"Threonine"},{"metabolite_name":"Trisaccharide"},{"metabolite_name":"Tyrosine"},{"metabolite_name":"Uracil"},{"metabolite_name":"Urea"},{"metabolite_name":"Uric Acid"},{"metabolite_name":"Valine"},{"metabolite_name":"Xanthine"}]
}

}