{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000687","ANALYSIS_ID":"AN001060","VERSION":"1","CREATED_ON":"12-15-2022"},
"PROJECT":{"PROJECT_TITLE":"Metabolic changes driven by Adenylate Kinase 4","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Identify metabolic changed driven by Adenylate Kinase 4","INSTITUTE":"Van Andel Research Institute","DEPARTMENT":"Center for Cancer and Cell Biology","LABORATORY":"MacKeigan Lab","LAST_NAME":"Lanning","FIRST_NAME":"Nate","ADDRESS":"333 Bostwick Ave N.E. Grand Rapids, MI 49503","EMAIL":"nate.lanning@vai.org","PHONE":"000-000-0000","DOI":"http://dx.doi.org/10.21228/M87G8X"},
"STUDY":{"STUDY_TITLE":"Nucleotide Analysis following Adenylate Kinase 4 knockdown","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"Nucleotide Analysis following Adenylate Kinase 4 knockdown","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","SUBMIT_DATE":"2017-06-19"},
"SUBJECT":{"SUBJECT_TYPE":"HUMAN","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","SPECIES_GROUP":"Human"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0002932",
"Sample ID":"S00012745",
"Factors":{"TYPE":"AK4"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012746",
"Factors":{"TYPE":"AK4"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012747",
"Factors":{"TYPE":"AK4"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012748",
"Factors":{"TYPE":"AK4"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012749",
"Factors":{"TYPE":"AK4"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012740",
"Factors":{"TYPE":"Control"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012741",
"Factors":{"TYPE":"Control"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012742",
"Factors":{"TYPE":"Control"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012743",
"Factors":{"TYPE":"Control"}
},
{
"Subject ID":"SU0002932",
"Sample ID":"S00012744",
"Factors":{"TYPE":"Control"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},
"TREATMENT":{"TREATMENT_SUMMARY":"-"},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A012_-_NAD-Related_Metabolites.pdf"},
"CHROMATOGRAPHY":{"INSTRUMENT_NAME":"Agilent","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm,3um)","FLOW_GRADIENT":"0min, 80%B, 15min, 0%B, 20min, 0%B, 35min, 80%B","FLOW_RATE":"75ul/min","SOLVENT_A":"100% water; 5mM ammonium acetate, pH 9.9","SOLVENT_B":"100% acetonitrile","CHROMATOGRAPHY_TYPE":"HILIC"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A012_-_NAD-Related_Metabolites.pdf"},
"MS":{"INSTRUMENT_NAME":"Agilent 6520B QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},
"MS_METABOLITE_DATA":{
"Units":"nmol/plate",
"Data":[{"Metabolite":"6-PHOSPHOGLUCONIC ACID","S00012745":"18.8825","S00012746":"22.3426","S00012747":"22.0474","S00012748":"18.6260","S00012749":"18.1497","S00012740":"26.1800","S00012741":"30.0971","S00012742":"19.7105","S00012743":"17.3406","S00012744":"19.7924"},{"Metabolite":"ACETOACETYL-COA","S00012745":"0.4968","S00012746":"0.5525","S00012747":"0.3976","S00012748":"0.2693","S00012749":"0.1459","S00012740":"0.5866","S00012741":"0.1759","S00012742":"0.3547","S00012743":"0.5356","S00012744":"0.4958"},{"Metabolite":"ADENOSINE 5^-DIPHOSPHATE","S00012745":"27.1465","S00012746":"26.0922","S00012747":"25.7087","S00012748":"24.4617","S00012749":"17.9655","S00012740":"45.9232","S00012741":"25.0778","S00012742":"16.2704","S00012743":"20.0361","S00012744":"34.6100"},{"Metabolite":"ADENOSINE 5^-MONOPHOSPHATE","S00012745":"3.7618","S00012746":"3.6164","S00012747":"3.5399","S00012748":"3.2844","S00012749":"2.0193","S00012740":"5.8099","S00012741":"3.0707","S00012742":"1.7410","S00012743":"2.7435","S00012744":"4.7229"},{"Metabolite":"ADENOSINE TRIPHOSPHATE","S00012745":"225.2333","S00012746":"239.8696","S00012747":"194.4392","S00012748":"259.5864","S00012749":"158.7814","S00012740":"180.4708","S00012741":"163.7025","S00012742":"186.2094","S00012743":"192.9822","S00012744":"225.1487"},{"Metabolite":"BETA-NAD+","S00012745":"14.6542","S00012746":"13.7282","S00012747":"18.4139","S00012748":"17.6925","S00012749":"15.4709","S00012740":"10.4543","S00012741":"12.0048","S00012742":"11.3392","S00012743":"14.1718","S00012744":"16.4510"},{"Metabolite":"CITRIC ACID","S00012745":"34.7718","S00012746":"44.1374","S00012747":"35.7117","S00012748":"46.0862","S00012749":"34.5927","S00012740":"44.8852","S00012741":"40.4716","S00012742":"40.6755","S00012743":"37.0812","S00012744":"42.8388"},{"Metabolite":"COENZYME II","S00012745":"1.4856","S00012746":"1.2530","S00012747":"1.0413","S00012748":"1.5383","S00012749":"0.8205","S00012740":"1.7662","S00012741":"1.0340","S00012742":"0.6220","S00012743":"0.5172","S00012744":"1.4784"},{"Metabolite":"D-FRUCTOSE_ 1_6-BIS(DIHYDROGEN PHOSPHATE)","S00012745":"32.0547","S00012746":"26.1498","S00012747":"35.4372","S00012748":"63.8442","S00012749":"46.2798","S00012740":"27.6266","S00012741":"34.0275","S00012742":"27.3182","S00012743":"21.1420","S00012744":"25.7175"},{"Metabolite":"FAD","S00012745":"0.5699","S00012746":"0.4570","S00012747":"0.6434","S00012748":"0.5433","S00012749":"0.5818","S00012740":"0.3653","S00012741":"0.4568","S00012742":"0.3858","S00012743":"0.5678","S00012744":"0.6282"},{"Metabolite":"GLYCERATE 3-PHOSPHATE","S00012745":"4.2525","S00012746":"3.5463","S00012747":"4.7835","S00012748":"5.3230","S00012749":"4.2295","S00012740":"4.6118","S00012741":"7.3726","S00012742":"4.2772","S00012743":"2.4509","S00012744":"4.5104"},{"Metabolite":"MALIC ACID","S00012745":"39.6053","S00012746":"38.0695","S00012747":"40.1153","S00012748":"47.7029","S00012749":"43.8176","S00012740":"37.4222","S00012741":"40.5989","S00012742":"37.2119","S00012743":"35.4745","S00012744":"41.7797"},{"Metabolite":"NADH","S00012745":"8.9246","S00012746":"9.6242","S00012747":"4.8381","S00012748":"3.6268","S00012749":"3.5262","S00012740":"17.1543","S00012741":"4.1087","S00012742":"3.8190","S00012743":"7.2403","S00012744":"13.1037"},{"Metabolite":"NADP-REDUCED","S00012745":"24.2535","S00012746":"31.9854","S00012747":"28.1294","S00012748":"26.6772","S00012749":"30.3010","S00012740":"44.2991","S00012741":"25.3814","S00012742":"24.6708","S00012743":"29.3079","S00012744":"31.7589"},{"Metabolite":"PHOSPHOENOLPYRUVATE","S00012745":"1.3078","S00012746":"0.8061","S00012747":"1.0918","S00012748":"0.9472","S00012749":"1.1922","S00012740":"2.7811","S00012741":"3.2109","S00012742":"2.1936","S00012743":"2.5256","S00012744":"1.6415"},{"Metabolite":"ROBISON ESTER","S00012745":"12.4237","S00012746":"12.0723","S00012747":"11.7056","S00012748":"16.2958","S00012749":"7.3525","S00012740":"9.4888","S00012741":"5.8337","S00012742":"8.3514","S00012743":"11.7879","S00012744":"12.9364"},{"Metabolite":"SUCROSE","S00012745":"9.2341","S00012746":"13.8915","S00012747":"7.9517","S00012748":"13.4576","S00012749":"8.0705","S00012740":"21.3511","S00012741":"8.0664","S00012742":"8.1463","S00012743":"7.7638","S00012744":"12.2450"}],
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}
}