{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000767","ANALYSIS_ID":"AN001213","VERSION":"1","CREATED_ON":"July 5, 2017, 12:57 pm"},

"PROJECT":{"PROJECT_TITLE":"Metabolic uniqueness of neural stem/progenitor cells Studies","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Cell-intrinsic metabolic uniqueness of regional neural stem/progenitor cells","INSTITUTE":"Cincinnati Children's Hospital Medical Center","DEPARTMENT":"Oncology","LABORATORY":"Dasgupta Lab","LAST_NAME":"Dasgupta","FIRST_NAME":"Biplab","ADDRESS":"Cincinnati, OH","EMAIL":"biplab.dasgupta@cchmc.org","PHONE":"513-803-1370"},

"STUDY":{"STUDY_TITLE":"Metabolite analysis of regional neural stem/progenitor cells","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"The developing mammalian brain generates a variety of Neural Progenitor Cells (NPCs). Primary NPCs throughout the neuraxis are derived from the ventricular zone. Intermediate progenitor cells (IPCs) are produced uniquely in the telencephalon and contribute extensively to the neurons that comprise the cerebral cortex and basal ganglia. It is known that the fate of the diverse NPC populations is determined by the interplay of transcription factors and regulation by regional humoral cues. However, despite our recent appreciation that nutrient-regulated intracellular metabolic milieu (pO2, energy, and redox state) significantly influence cell fate, an unexplored area is whether NPCs have intrinsic metabolic identity, and if so, the mechanism by which molecular metabolism contributes to brain development.Little is known however, if intrinsic differences in cellular metabolism of regional NPCs make certain NPCs susceptible while others resistant to genetic and environmental insults. We conjectured that regional (fore-and hindbrain) NPCs are metabolically distinct.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"2","TOTAL_SUBJECTS":"4"},

"SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0016334",
"Sample ID":"S00026132",
"Factors":{"NPC source":"forebrain"}
},
{
"Subject ID":"SU0016335",
"Sample ID":"S00026133",
"Factors":{"NPC source":"forebrain"}
},
{
"Subject ID":"SU0016336",
"Sample ID":"S00026134",
"Factors":{"NPC source":"hindbrain"}
},
{
"Subject ID":"SU0016337",
"Sample ID":"S00026135",
"Factors":{"NPC source":"hindbrain"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011 - Glycolysis-TCA-nucleotides.pdf"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6520B QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/mg protein",

"Data":[{"Metabolite":"PHOSPHOENOLPYRUVATE","S00026132":"3.886997781","S00026133":"5.398145468","S00026134":"4.059950455","S00026135":"3.092003803"},{"Metabolite":"D-ERYTHROSE 4-PHOSPHATE","S00026132":"20.34995022","S00026133":"14.79423862","S00026134":"25.53561269","S00026135":"18.74621727"},{"Metabolite":"BETA-NAD+","S00026132":".299008893","S00026133":".263429119","S00026134":".804146524","S00026135":".678213187"},{"Metabolite":"COENZYME II","S00026132":".637049112","S00026133":".215437899","S00026134":".178526499","S00026135":".267162046"},{"Metabolite":"SEDOHEPTULOSE 7-PHOSPHATE","S00026132":"3.904039503","S00026133":"2.224604138","S00026134":"4.123699279","S00026135":"3.869276462"},{"Metabolite":"D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE)","S00026132":"6.085621569","S00026133":"1.854668387","S00026134":"1.871040565","S00026135":"3.606827328"},{"Metabolite":"NADP-REDUCED","S00026132":"0","S00026133":"0","S00026134":"0","S00026135":"0"},{"Metabolite":"CITRIC ACID","S00026132":"48.5491184","S00026133":"11.58314121","S00026134":"23.44760015","S00026135":"20.52089241"},{"Metabolite":"NADH","S00026132":"19.23263829","S00026133":".193968941","S00026134":".553692434","S00026135":".644093146"},{"Metabolite":"FAD","S00026132":".175350183","S00026133":".169438275","S00026134":".404843723","S00026135":".398166614"},{"Metabolite":"MALIC ACID","S00026132":"10.70852479","S00026133":"16.72993796","S00026134":"43.66932609","S00026135":"48.47435831"},{"Metabolite":"ADENOSINE TRIPHOSPHATE","S00026132":"3.402329405","S00026133":"1.44722611","S00026134":"12.0012695","S00026135":"11.03019019"},{"Metabolite":"ROBISON ESTER","S00026132":"23.75964361","S00026133":"22.19374424","S00026134":"62.04572981","S00026135":"41.69657121"},{"Metabolite":"SUCROSE","S00026132":"4.800300233","S00026133":"4.142997676","S00026134":"5.892170226","S00026135":"9.778024279"},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","S00026132":"3.766189115","S00026133":"1.433392119","S00026134":"19.00124464","S00026135":"24.66291966"},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","S00026132":"19.26190717","S00026133":"20.31379598","S00026134":"51.76080582","S00026135":"48.27363017"},{"Metabolite":"GLYCERATE 3-PHOSPHATE","S00026132":"47.59116421","S00026133":"41.81434839","S00026134":"44.03546965","S00026135":"41.88394658"},{"Metabolite":"RIBOSE PHOSPHATE","S00026132":"5.96044819","S00026133":"4.993082081","S00026134":"10.70262238","S00026135":"10.4523611"},{"Metabolite":"6-PHOSPHOGLUCONIC ACID","S00026132":"7.98731687","S00026133":"5.637812928","S00026134":"4.349344647","S00026135":"5.072449283"},{"Metabolite":"ACETOACETYL-COA","S00026132":"0","S00026133":"0","S00026134":"0","S00026135":"0"}],

"Metabolites":[{"Metabolite":"PHOSPHOENOLPYRUVATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1005","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"D-ERYTHROSE 4-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"122357","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"BETA-NAD+","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"15938971","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"COENZYME II","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"15938972","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"SEDOHEPTULOSE 7-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"165007","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"21125049","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"NADP-REDUCED","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"22833512","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"CITRIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"311","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"NADH","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"439153","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"FAD","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"46906035","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"MALIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"525","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE TRIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5957","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ROBISON ESTER","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5958","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"SUCROSE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5988","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6022","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6083","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"GLYCERATE 3-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"724","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"RIBOSE PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"77982","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"6-PHOSPHOGLUCONIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"91493","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ACETOACETYL-COA","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"92153","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}]
}

}