{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000833","ANALYSIS_ID":"AN001841","VERSION":"1","CREATED_ON":"January 16, 2019, 9:11 am"},

"PROJECT":{"PROJECT_TITLE":"Influence of murine norovirus on cell metabolism","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"C6 effects in RAW","INSTITUTE":"University of Michigan","DEPARTMENT":"Microbiology & Immunology","LABORATORY":"Passalacqua Lab","LAST_NAME":"Passalacqua","FIRST_NAME":"Karla","ADDRESS":"Ann Arbor, MI","EMAIL":"kpassal@umich.edu","PHONE":"734-763-5639"},

"STUDY":{"STUDY_TITLE":"Influence of murine norovirus on cell metabolism","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"6.5 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of medium containing either Murine Norovirus-1 (MNV) at an MOI=5 or medium containing a v/v match of mock lysate was added to the cells. Plates were rocked on ice for 1 hour, then cells were washed 3X with cold DPBS++, and plain medium as added to cells. Plates were then incubated for 7.5 hours at 37 degrees and 5% CO2. Cells were washed with 12 mL of 150 mM Ammonium Acetate, swirled 8 times, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"2","TOTAL_SUBJECTS":"1","STUDY_COMMENTS":"BIOHAZARD - MURINE NOROVIRUS - SHOULD BE ENTIRELY NON-INFECTIOUS DUE TO THE LIQUID NITROGEN, BUT PLEASE HANDLE AND DISPOSE OF IN BIOHAZARD WASTE. IMPORTANT NOTE: I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P H6P and Lactate. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data."},

"SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0015382",
"Sample ID":"S00023719",
"Factors":{"Medium":"mock lysate"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023720",
"Factors":{"Medium":"mock lysate"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023721",
"Factors":{"Medium":"mock lysate"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023722",
"Factors":{"Medium":"MNV infected"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023723",
"Factors":{"Medium":"MNV infected"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023724",
"Factors":{"Medium":"MNV infected"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011_Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent","COLUMN_NAME":"Phenomenex Luna NH2 (150 x 1mm, 3um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011_Glycolysis-TCA-nucleotides.pdf","ACQUISITION_PARAMETERS_FILE":"QTOF-002-HILIC-35min-1mm.m"},

"MS":{"INSTRUMENT_NAME":"Agilent 6520B QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"none"},

"MS_METABOLITE_DATA":{
"Units":"uM",

"Data":[{"Metabolite":"D-ERYTHROSE 4-PHOSPHATE","S00023719":"2.613316001","S00023720":"9.39460765","S00023721":"6.617425743","S00023722":"2.686075161","S00023723":"3.637145004","S00023724":"2.671098354"},{"Metabolite":"BETA-NAD+","S00023719":".8411","S00023720":".6508","S00023721":".9522","S00023722":".3537","S00023723":".3948","S00023724":".8944"},{"Metabolite":"COENZYME II","S00023719":".35353663","S00023720":".270743602","S00023721":".209430055","S00023722":".34205688","S00023723":".254861566","S00023724":".220849974"},{"Metabolite":"SEDOHEPTULOSE 7-PHOSPHATE","S00023719":".294101064","S00023720":".150612803","S00023721":".200439074","S00023722":".340670815","S00023723":".258101705","S00023724":".220061428"},{"Metabolite":"D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE)","S00023719":".537716579","S00023720":".215974879","S00023721":".240757235","S00023722":".28408073","S00023723":".211737232","S00023724":".215282397"},{"Metabolite":"CITRIC ACID","S00023719":"8.839184579","S00023720":"9.457720082","S00023721":"7.454542888","S00023722":"9.680821855","S00023723":"8.786287367","S00023724":"7.032517805"},{"Metabolite":"FAD","S00023719":".05256291","S00023720":".032642277","S00023721":".018653194","S00023722":".042580693","S00023723":".029364375","S00023724":".029519873"},{"Metabolite":"MALIC ACID","S00023719":"5.345311821","S00023720":"1.637526539","S00023721":".817815478","S00023722":"4.482918214","S00023723":"1.81515342","S00023724":"2.179012735"},{"Metabolite":"ADENOSINE TRIPHOSPHATE","S00023719":"70.52576481","S00023720":"23.12110245","S00023721":"100.1014","S00023722":"66.1705","S00023723":"57.4472","S00023724":"74.8022"},{"Metabolite":"ROBISON ESTER","S00023719":"2.964311528","S00023720":"1.002351981","S00023721":"1.497850283","S00023722":"2.893330557","S00023723":"1.86805967","S00023724":"1.897242409"},{"Metabolite":"SUCROSE","S00023719":"4.553443839","S00023720":"5.817576358","S00023721":"4.736623481","S00023722":"5.671434936","S00023723":"5.745789359","S00023724":"3.81054713"},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","S00023719":"5.347603573","S00023720":"9.441332401","S00023721":"7.818744872","S00023722":"8.256676598","S00023723":"8.088915322","S00023724":"6.294614524"},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","S00023719":"1.378915024","S00023720":"6.550610103","S00023721":"5.286045776","S00023722":"1.709110261","S00023723":"2.03119849","S00023724":"1.695720049"},{"Metabolite":"6-PHOSPHOGLUCONIC ACID","S00023719":".217027862","S00023720":".209660448","S00023721":".20478464","S00023722":".21731253","S00023723":".206793637","S00023724":".206596261"}],

"Metabolites":[{"Metabolite":"D-ERYTHROSE 4-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"122357","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"BETA-NAD+","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"15938971","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"COENZYME II","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"15938972","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"SEDOHEPTULOSE 7-PHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"165007","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"D-FRUCTOSE, 1,6-BIS(DIHYDROGEN PHOSPHATE)","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"21125049","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"CITRIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"311","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"FAD","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"46906035","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"MALIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"525","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE TRIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5957","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ROBISON ESTER","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5958","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"SUCROSE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"5988","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-DIPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6022","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"ADENOSINE 5'-MONOPHOSPHATE","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"6083","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"6-PHOSPHOGLUCONIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"91493","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}]
}

}