{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000833","ANALYSIS_ID":"AN001842","VERSION":"1","CREATED_ON":"January 16, 2019, 9:11 am"},

"PROJECT":{"PROJECT_TITLE":"Influence of murine norovirus on cell metabolism","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"C6 effects in RAW","INSTITUTE":"University of Michigan","DEPARTMENT":"Microbiology & Immunology","LABORATORY":"Passalacqua Lab","LAST_NAME":"Passalacqua","FIRST_NAME":"Karla","ADDRESS":"Ann Arbor, MI","EMAIL":"kpassal@umich.edu","PHONE":"734-763-5639"},

"STUDY":{"STUDY_TITLE":"Influence of murine norovirus on cell metabolism","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"6.5 X 10^6 cells were plated in 10 cm Tissue Culture dishes and allowed to recover overnight at 37 degrees and 5% CO2. In the am, supernatant was removed, and 12 mL of medium containing either Murine Norovirus-1 (MNV) at an MOI=5 or medium containing a v/v match of mock lysate was added to the cells. Plates were rocked on ice for 1 hour, then cells were washed 3X with cold DPBS++, and plain medium as added to cells. Plates were then incubated for 7.5 hours at 37 degrees and 5% CO2. Cells were washed with 12 mL of 150 mM Ammonium Acetate, swirled 8 times, and immediately quenched with liquid nitrogen. Cells were then frozen at -80 degrees.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"2","TOTAL_SUBJECTS":"1","STUDY_COMMENTS":"BIOHAZARD - MURINE NOROVIRUS - SHOULD BE ENTIRELY NON-INFECTIOUS DUE TO THE LIQUID NITROGEN, BUT PLEASE HANDLE AND DISPOSE OF IN BIOHAZARD WASTE. IMPORTANT NOTE: I request that the metabolites of the TCA+ assay that are to be assessed quantitatively please be sure to include ATP, ADP, AMP, NAD, NADH, NADP, NADPH, E4P, S7P, 6PG, G3P H6P and Lactate. Also, I would request that both reduced and oxidized Glutathione as well as Fructose 1,6-bisphosphate be included in the non-quantitative data."},

"SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0015382",
"Sample ID":"S00023719",
"Factors":{"Medium":"mock lysate"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023720",
"Factors":{"Medium":"mock lysate"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023721",
"Factors":{"Medium":"mock lysate"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023722",
"Factors":{"Medium":"MNV infected"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023723",
"Factors":{"Medium":"MNV infected"}
},
{
"Subject ID":"SU0015382",
"Sample ID":"S00023724",
"Factors":{"Medium":"MNV infected"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011_Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent GC_7890N","COLUMN_NAME":"Agilent HP5-MS (15m × 0.25mm, 0.25 um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011_Glycolysis-TCA-nucleotides.pdf","ACQUISITION_PARAMETERS_FILE":"HEIDIFAMES.M"},

"MS":{"INSTRUMENT_NAME":"Agilent MS_5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"none"},

"MS_METABOLITE_DATA":{
"Units":"pmol/ug protein",

"Data":[{"Metabolite":"PYRUVIC ACID","S00023719":"9.301541105","S00023720":"14.90496856","S00023721":"14.99708502","S00023722":"11.80735452","S00023723":"9.116197265","S00023724":"5.77155209"},{"Metabolite":"FUMARIC ACID","S00023719":"36.17005854","S00023720":"38.38719496","S00023721":"26.84813761","S00023722":"53.70050442","S00023723":"33.11044567","S00023724":"19.60659917"},{"Metabolite":"LACTIC ACID","S00023719":"2435.484483","S00023720":"2288.154623","S00023721":"1724.146814","S00023722":"2678.46717","S00023723":"2016.551207","S00023724":"1280.271163"}],

"Metabolites":[{"Metabolite":"PYRUVIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1060","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"FUMARIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"444972","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"LACTIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"612","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}]
}

}