{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000840","ANALYSIS_ID":"AN001845","VERSION":"1","CREATED_ON":"January 16, 2019, 9:33 am"},

"PROJECT":{"PROJECT_TITLE":"Diabetic Heart","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Diabetic heart adrenergic signaling and metabolism","INSTITUTE":"Oklahoma Medical Research Foundation","DEPARTMENT":"Aging & Metabolism","LABORATORY":"Humphries Lab","LAST_NAME":"Humphries","FIRST_NAME":"Kenneth","ADDRESS":"Oklahoma City, OK","EMAIL":"Kenneth-Humphries@omrf.org","PHONE":"405-271-7584"},

"STUDY":{"STUDY_TITLE":"Diabetic heart adrenergic signaling and metabolism (STZ & Isoproterenol, High Fat)","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"We are examing the effect of both diabetes and adrenergic activation on the metabolomic profile. STZ treatment was initiated 5 months before hearts were harvested. High fat/low fat diets were given for 13 weeks. All hearts were excised in the morning. For the STZ/isoproterenol experiment, mouse anesthesia was initiated with 4% isoflurane, and maintained with 2% and a homeothermic controller. After 5 min of equilibration, control and fasted mice were injected intraperitoneally with either saline (S) or 25ug/kg isoproterenol (I). After an additional 5 min, the whole heart was quickly excised, washed with ice-cold distilled water, dried off with a chem whipe, and flash frozen in liquid nitrogen. Hearts were stored at -80, and shipped using dry ice. The hearts from the high fat diet experiement were prepared in the same way, but no injection was given and total anesthesia time was 5min instead of 10min.","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714","EMAIL":"mkachman@med.umich.edu","PHONE":"(734) 232-8175","NUM_GROUPS":"7","TOTAL_SUBJECTS":"29"},

"SUBJECT":{"SUBJECT_TYPE":"MOUSE","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"SU0014674",
"Sample ID":"S00022376",
"Factors":{"Treatment":"Saline","Type":"Control"}
},
{
"Subject ID":"SU0014675",
"Sample ID":"S00022377",
"Factors":{"Treatment":"Saline","Type":"Control"}
},
{
"Subject ID":"SU0014676",
"Sample ID":"S00022378",
"Factors":{"Treatment":"Saline","Type":"Control"}
},
{
"Subject ID":"SU0014677",
"Sample ID":"S00022379",
"Factors":{"Treatment":"Saline","Type":"Control"}
},
{
"Subject ID":"SU0014678",
"Sample ID":"S00022380",
"Factors":{"Treatment":"Saline","Type":"Control"}
},
{
"Subject ID":"SU0014679",
"Sample ID":"S00022381",
"Factors":{"Treatment":"Saline","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014680",
"Sample ID":"S00022382",
"Factors":{"Treatment":"Saline","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014681",
"Sample ID":"S00022383",
"Factors":{"Treatment":"Saline","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014682",
"Sample ID":"S00022384",
"Factors":{"Treatment":"Saline","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014683",
"Sample ID":"S00022385",
"Factors":{"Treatment":"Saline","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014684",
"Sample ID":"S00022386",
"Factors":{"Treatment":"Isoproterenol","Type":"Control"}
},
{
"Subject ID":"SU0014685",
"Sample ID":"S00022387",
"Factors":{"Treatment":"Isoproterenol","Type":"Control"}
},
{
"Subject ID":"SU0014686",
"Sample ID":"S00022388",
"Factors":{"Treatment":"Isoproterenol","Type":"Control"}
},
{
"Subject ID":"SU0014687",
"Sample ID":"S00022389",
"Factors":{"Treatment":"Isoproterenol","Type":"Control"}
},
{
"Subject ID":"SU0014688",
"Sample ID":"S00022390",
"Factors":{"Treatment":"Isoproterenol","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014689",
"Sample ID":"S00022391",
"Factors":{"Treatment":"Isoproterenol","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014690",
"Sample ID":"S00022392",
"Factors":{"Treatment":"Isoproterenol","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014691",
"Sample ID":"S00022393",
"Factors":{"Treatment":"Isoproterenol","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014692",
"Sample ID":"S00022394",
"Factors":{"Treatment":"Isoproterenol","Type":"STZ diabetic"}
},
{
"Subject ID":"SU0014693",
"Sample ID":"S00022395",
"Factors":{"Treatment":"none","Type":"Low fat diet"}
},
{
"Subject ID":"SU0014694",
"Sample ID":"S00022396",
"Factors":{"Treatment":"none","Type":"Low fat diet"}
},
{
"Subject ID":"SU0014695",
"Sample ID":"S00022397",
"Factors":{"Treatment":"none","Type":"Low fat diet"}
},
{
"Subject ID":"SU0014696",
"Sample ID":"S00022398",
"Factors":{"Treatment":"none","Type":"Low fat diet"}
},
{
"Subject ID":"SU0014697",
"Sample ID":"S00022399",
"Factors":{"Treatment":"none","Type":"Low fat diet"}
},
{
"Subject ID":"SU0014698",
"Sample ID":"S00022400",
"Factors":{"Treatment":"none","Type":"High fat diet"}
},
{
"Subject ID":"SU0014699",
"Sample ID":"S00022401",
"Factors":{"Treatment":"none","Type":"High fat diet"}
},
{
"Subject ID":"SU0014700",
"Sample ID":"S00022402",
"Factors":{"Treatment":"none","Type":"High fat diet"}
},
{
"Subject ID":"SU0014701",
"Sample ID":"S00022403",
"Factors":{"Treatment":"none","Type":"High fat diet"}
},
{
"Subject ID":"SU0014702",
"Sample ID":"S00022404",
"Factors":{"Treatment":"none","Type":"High fat diet"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"-","SAMPLE_TYPE":"Heart"},

"TREATMENT":{"TREATMENT_SUMMARY":"-"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"-","SAMPLEPREP_PROTOCOL_FILENAME":"A011_Glycolysis-TCA-nucleotides.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"GC","INSTRUMENT_NAME":"Agilent GC_7890N","COLUMN_NAME":"Agilent DB5-MS (30m × 0.25mm, 0.25um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"A011_Glycolysis-TCA-nucleotides.pdf","ACQUISITION_PARAMETERS_FILE":"ALPHA KETO ACIDS-FULL.M"},

"MS":{"INSTRUMENT_NAME":"Agilent MS_5975","INSTRUMENT_TYPE":"Single quadrupole","MS_TYPE":"EI","ION_MODE":"POSITIVE","MS_COMMENTS":"none"},

"MS_METABOLITE_DATA":{
"Units":"nmol/mg",

"Data":[{"Metabolite":"PYRUVIC ACID","S00022376":".002690792","S00022377":".002306379","S00022378":".002399577","S00022379":".002241308","S00022380":".002073886","S00022381":".00236583","S00022382":".003178321","S00022383":".002318982","S00022384":".002672803","S00022385":".002911504","S00022386":".001839684","S00022387":".002116572","S00022388":".001888965","S00022389":".002122809","S00022390":".002448401","S00022391":".002093815","S00022392":".002622569","S00022393":".002181108","S00022394":".002878324","S00022395":".001423068","S00022396":".001812885","S00022397":".001955237","S00022398":".002398084","S00022399":".002230441","S00022400":".002897453","S00022401":".003293048","S00022402":".002876439","S00022403":".003012973","S00022404":".002457201"},{"Metabolite":"FUMARIC ACID","S00022376":".008144423","S00022377":".00592678","S00022378":".007117805","S00022379":".007297742","S00022380":".006704043","S00022381":".01205887","S00022382":".01053015","S00022383":".012073034","S00022384":".01051359","S00022385":".011093094","S00022386":".009155805","S00022387":".009055551","S00022388":".012555645","S00022389":".011036272","S00022390":".017066227","S00022391":".025268475","S00022392":".023782575","S00022393":".021773673","S00022394":".018682154","S00022395":".010813554","S00022396":".009892856","S00022397":".016325485","S00022398":".013554695","S00022399":".012155283","S00022400":".008546986","S00022401":".009133208","S00022402":".008051552","S00022403":".010220977","S00022404":".014689737"},{"Metabolite":"LACTIC ACID","S00022376":"2.205714186","S00022377":"2.262276034","S00022378":"1.984009675","S00022379":"2.551883202","S00022380":"1.942897671","S00022381":"2.206353908","S00022382":"3.200220849","S00022383":"2.376348998","S00022384":"2.678360616","S00022385":"2.332013786","S00022386":"1.58746671","S00022387":"2.030229717","S00022388":"1.706405406","S00022389":"2.075274799","S00022390":"1.739396761","S00022391":"1.789420107","S00022392":"2.082853441","S00022393":"2.146282546","S00022394":"2.643452685","S00022395":"2.300591919","S00022396":"2.152605693","S00022397":".667909297","S00022398":"2.581113608","S00022399":"3.122829957","S00022400":"4.270708918","S00022401":"4.565542431","S00022402":"4.113164025","S00022403":"3.534402309","S00022404":"2.963567661"}],

"Metabolites":[{"Metabolite":"PYRUVIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"1060","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"FUMARIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"444972","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""},{"Metabolite":"LACTIC ACID","moverz_quant":"","ri":"","ri_type":"","pubchem_id":"612","inchi_key":"","kegg_id":"","other_id":"","other_id_type":""}]
}

}