{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000845","ANALYSIS_ID":"AN001368","VERSION":"1","CREATED_ON":"August 10, 2017, 2:52 pm"},

"PROJECT":{"PROJECT_TITLE":"Statin Immuno-Metabolomics in Asthma","PROJECT_TYPE":"Placebo-controled trial","PROJECT_SUMMARY":"Innovative and novel therapies are urgently needed for the treatment of patients with severe asthma, especially those who are refractory to standard-of-care bronchodilators and inhaled corticosteroids. The Zeki lab is investigating the role of the mevalonate (MA) pathway, in the pathogenesis of airway inflammation and remodeling. Although statins all inhibit HMGCR in the same manner in terms of enzyme binding site, the statins’ varied physiochemical properties with respect to their polarity (i.e. lipophilicity) result in very different immune and lipid effects. The major significance of this work is to advance a new class of inhaler therapies for asthma; the statins which work by an entirely different mechanism than current ICS/LABA mainstays. Evidence suggests that statins may have an additive benefit to corticosteroids in asthma, thereby confirming a unique mechanism, namely via MVA pathway inhibition. This becomes particularly important in the severe asthma population which is highly corticosteroid-resistant, is poorly controlled with high exacerbation rates and hospitalizations, and has the highest healthcare costs of all asthma phenotypes. In essence, the potential public health impact of even an incremental improvement in asthma symptom control cannot be underestimated. Even the prevention of 1 asthma attack preserves lung function and reduces the adverse personal and financial impact. This study aimed to determine if statin polarity affects airway drug concentration and systemic drug absorption and to determine the effect of inhaled statins on naïve airway immune cell populations and alveolar-capillary membrane and epithelial barrier integrity in healthy rhesus monkeys. In this particular component of the study, we investigated the metabolic effects resulting from the use of statins in these healthy rhesus monkeys. Specifically, the Newman lab analyzed for lipid mediator (oxylipin, endocannabinoid, fatty acid, and nitro lipid) in lung and trachea tissue, plasma, and BAL and bile acid changes in the lung and trachea tissue and plasma.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Internal Medicine","LAST_NAME":"Zeki","FIRST_NAME":"Amir","ADDRESS":"2825 J St. Suite 400 Sacramento, CA 95816","EMAIL":"aazeki@ucdavis.edu","PHONE":"(916) 734-8230"},

"STUDY":{"STUDY_TITLE":"Statin Immuno-Metabolomics in Asthma (part III)","STUDY_TYPE":"Placebo-controled trial","STUDY_SUMMARY":"Innovative and novel therapies are urgently needed for the treatment of patients with severe asthma, especially those who are refractory to standard-of-care bronchodilators and inhaled corticosteroids. The Zeki lab is investigating the role of the mevalonate (MA) pathway, in the pathogenesis of airway inflammation and remodeling. Although statins all inhibit HMGCR in the same manner in terms of enzyme binding site, the statins’ varied physiochemical properties with respect to their polarity (i.e. lipophilicity) result in very different immune and lipid effects. The major significance of this work is to advance a new class of inhaler therapies for asthma; the statins which work by an entirely different mechanism than current ICS/LABA mainstays. Evidence suggests that statins may have an additive benefit to corticosteroids in asthma, thereby confirming a unique mechanism, namely via MVA pathway inhibition. This becomes particularly important in the severe asthma population which is highly corticosteroid-resistant, is poorly controlled with high exacerbation rates and hospitalizations, and has the highest healthcare costs of all asthma phenotypes. In essence, the potential public health impact of even an incremental improvement in asthma symptom control cannot be underestimated. Even the prevention of 1 asthma attack preserves lung function and reduces the adverse personal and financial impact. This study aimed to determine if statin polarity affects airway drug concentration and systemic drug absorption and to determine the effect of inhaled statins on naïve airway immune cell populations and alveolar-capillary membrane and epithelial barrier integrity in healthy rhesus monkeys. In this particular component of the study, we investigated the metabolic effects resulting from the use of statins in these healthy rhesus monkeys. Specifically, the Newman lab analyzed for lipid mediator (oxylipin, endocannabinoid, fatty acid, and nitro lipid) in lung and trachea tissue, plasma, and BAL and bile acid changes in the lung and trachea tissue and plasma.","INSTITUTE":"USDA","DEPARTMENT":"Obesity and metabolism research unit","LAST_NAME":"Newman","FIRST_NAME":"John","ADDRESS":"430 West Health Sciences Dr. Davis, Ca, 95616","EMAIL":"John.Newman@ars.usda.gov","PHONE":"(530) 752-1009"},

"SUBJECT":{"SUBJECT_TYPE":"Animal","SUBJECT_SPECIES":"Macaca mulatta","TAXONOMY_ID":"9544"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"PLAZ-14",
"Factors":{"Treatment":"Control","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-32",
"Factors":{"Treatment":"Control","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-23",
"Factors":{"Treatment":"Control","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-09",
"Factors":{"Treatment":"Simvastatin","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-19",
"Factors":{"Treatment":"Simvastatin","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-26",
"Factors":{"Treatment":"Simvastatin","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-04",
"Factors":{"Treatment":"Control","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-34",
"Factors":{"Treatment":"Control","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-08",
"Factors":{"Treatment":"Control","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-25",
"Factors":{"Treatment":"Simvastatin","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-13",
"Factors":{"Treatment":"Simvastatin","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-06 Rep Avg.",
"Factors":{"Treatment":"Simvastatin","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-29",
"Factors":{"Treatment":"Control","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-11",
"Factors":{"Treatment":"Control","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-05",
"Factors":{"Treatment":"Control","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-10",
"Factors":{"Treatment":"Simvastatin","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-21",
"Factors":{"Treatment":"Simvastatin","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-35",
"Factors":{"Treatment":"Simvastatin","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-33",
"Factors":{"Treatment":"Control","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-31",
"Factors":{"Treatment":"Control","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-20",
"Factors":{"Treatment":"Control","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-24",
"Factors":{"Treatment":"Pravastatin","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-36",
"Factors":{"Treatment":"Pravastatin","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-22",
"Factors":{"Treatment":"Pravastatin","Day":"Day 0"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-18",
"Factors":{"Treatment":"Control","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-15",
"Factors":{"Treatment":"Control","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-17",
"Factors":{"Treatment":"Control","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-27",
"Factors":{"Treatment":"Pravastatin","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-02",
"Factors":{"Treatment":"Pravastatin","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-07 Rep Avg.",
"Factors":{"Treatment":"Pravastatin","Day":"Day 8"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-12",
"Factors":{"Treatment":"Control","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-01",
"Factors":{"Treatment":"Control","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-30",
"Factors":{"Treatment":"Control","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-28",
"Factors":{"Treatment":"Pravastatin","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-16",
"Factors":{"Treatment":"Pravastatin","Day":"Day 12"}
},
{
"Subject ID":"-",
"Sample ID":"PLAZ-03",
"Factors":{"Treatment":"Pravastatin","Day":"Day 12"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Monkeys were treated with placebo or Provastatin for 12 days. Further, after the wash out period animals were treated with Simvastatin for 12 days. Plasma was collected at day 0, 8 and 12 of each treatment.","SAMPLE_TYPE":"Blood","BLOOD_SERUM_OR_PLASMA":"Plasma"},

"TREATMENT":{"TREATMENT_SUMMARY":"Monkeys were treated (by inhalation) with placebo or Provastatin for 12 days. Further, after the wash out period animals were treated with Simvastatin for 12 days. Plasma was collected at day 0, 8 and 12 of each treatment."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Oxylipins, endocannabinoids, bile acids and fatty acids were isolated from an aliquot of 20μl plasma in Eppendorf tubes. The tube was spiked was 5ul of OxyEndo Fusion, 10μl spike of Bile Acid SSTD Solutions, and 5μl of BHT/EDTA solution. A total of 100μl CUDA/PHAU in methanol was added to the tube, before it was capped, vortexed, and centrifuged (3 min, 10,000 RCF, room temp). It was then placed on wet ice for 5 min. Next it was spin filtered with Millapore spin filters (0.1 µm, Millipore, Billerica, MA) at 8 ºC, 4500 RPM for 3 min, before being transferred to 2 mL LC-MS amber vials. Extracts were stored at -20ºC until analysis by UPLC-MS/MS. The internal standard was used to quantify the recovery of surrogate standards."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Aquity C18 BEH 1.7µm 100mm x 2.1mm column","FLOW_GRADIENT":"See protocol/methods file","FLOW_RATE":"0.4 mL/min","COLUMN_TEMPERATURE":"60 °C","SOLVENT_A":"0.1% Formic Acid","SOLVENT_B":"0.1% Formic Acid in Acetonitrile","INTERNAL_STANDARD":"See protocol/methods file","RETENTION_TIME":"See protocol/methods file","SAMPLE_INJECTION":"5 µL","ANALYTICAL_TIME":"16 min","WEAK_WASH_SOLVENT_NAME":"20% methanol, 10% isopropanol","WEAK_WASH_VOLUME":"600 µL","STRONG_WASH_SOLVENT_NAME":"50:50 Acetonitrile:Methanol","STRONG_WASH_VOLUME":"600 µL"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS","LABORATORY_NAME":"Newman","OPERATOR_NAME":"Kamil Borkowski","DETECTOR_TYPE":"API 6500 QTrap (AB Sciex, Framingham, MA, USA)","SOFTWARE_VERSION":"AB Sciex MultiQuant version 3.0.2","DATA_FORMAT":".mzML"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"ABI Sciex 6500 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"NEGATIVE"},

"MS_METABOLITE_DATA":{
"Units":"Concentration (nM)",

"Data":[{"Metabolite":"CA","PLAZ-14":"32.9","PLAZ-32":"103","PLAZ-23":"ND","PLAZ-09":"549","PLAZ-19":"156","PLAZ-26":"43.3","PLAZ-04":"60.6","PLAZ-34":"81.2","PLAZ-08":"39.9","PLAZ-25":"722","PLAZ-13":"76","PLAZ-06 Rep Avg.":"27.1","PLAZ-29":"108","PLAZ-11":"257","PLAZ-05":"2.8","PLAZ-10":"119","PLAZ-21":"34.7","PLAZ-35":"15.3","PLAZ-33":"443","PLAZ-31":"59.7","PLAZ-20":"33.2","PLAZ-24":"98.2","PLAZ-36":"6580","PLAZ-22":"15.7","PLAZ-18":"57.3","PLAZ-15":"1660","PLAZ-17":"1590","PLAZ-27":"182","PLAZ-02":"139","PLAZ-07 Rep Avg.":"42.8","PLAZ-12":"13.7","PLAZ-01":"64.1","PLAZ-30":"1.51","PLAZ-28":"57.9","PLAZ-16":"24.2","PLAZ-03":"9.36"},{"Metabolite":"CDCA","PLAZ-14":"115","PLAZ-32":"576","PLAZ-23":"34.4","PLAZ-09":"344","PLAZ-19":"769","PLAZ-26":"135","PLAZ-04":"461","PLAZ-34":"389","PLAZ-08":"741","PLAZ-25":"763","PLAZ-13":"2120","PLAZ-06 Rep Avg.":"153","PLAZ-29":"1560","PLAZ-11":"1190","PLAZ-05":"66.2","PLAZ-10":"221","PLAZ-21":"143","PLAZ-35":"103","PLAZ-33":"426","PLAZ-31":"294","PLAZ-20":"182","PLAZ-24":"163","PLAZ-36":"11900","PLAZ-22":"ND","PLAZ-18":"320","PLAZ-15":"2410","PLAZ-17":"3330","PLAZ-27":"791","PLAZ-02":"2320","PLAZ-07 Rep Avg.":"166","PLAZ-12":"58","PLAZ-01":"237","PLAZ-30":"66.7","PLAZ-28":"162","PLAZ-16":"284","PLAZ-03":"103"},{"Metabolite":"TCA","PLAZ-14":"335","PLAZ-32":"278","PLAZ-23":"4.35","PLAZ-09":"325","PLAZ-19":"80.4","PLAZ-26":"4690","PLAZ-04":"12.8","PLAZ-34":"68.4","PLAZ-08":"0.458","PLAZ-25":"36.9","PLAZ-13":"ND","PLAZ-06 Rep Avg.":"37.45","PLAZ-29":"241","PLAZ-11":"97.5","PLAZ-05":"57.1","PLAZ-10":"23.6","PLAZ-21":"ND","PLAZ-35":"125","PLAZ-33":"80.2","PLAZ-31":"238","PLAZ-20":"34.7","PLAZ-24":"175","PLAZ-36":"1140","PLAZ-22":"632","PLAZ-18":"11.8","PLAZ-15":"168","PLAZ-17":"5.43","PLAZ-27":"19.3","PLAZ-02":"57","PLAZ-07 Rep Avg.":"249.5","PLAZ-12":"75.8","PLAZ-01":"367","PLAZ-30":"8.04","PLAZ-28":"44.3","PLAZ-16":"25.2","PLAZ-03":"309"},{"Metabolite":"TCDCA","PLAZ-14":"487","PLAZ-32":"546","PLAZ-23":"25.5","PLAZ-09":"535","PLAZ-19":"276","PLAZ-26":"4180","PLAZ-04":"83.4","PLAZ-34":"388","PLAZ-08":"33.7","PLAZ-25":"135","PLAZ-13":"41","PLAZ-06 Rep Avg.":"112.5","PLAZ-29":"613","PLAZ-11":"281","PLAZ-05":"219","PLAZ-10":"71.8","PLAZ-21":"5.1","PLAZ-35":"117","PLAZ-33":"128","PLAZ-31":"530","PLAZ-20":"131","PLAZ-24":"444","PLAZ-36":"2360","PLAZ-22":"995","PLAZ-18":"78.8","PLAZ-15":"899","PLAZ-17":"31","PLAZ-27":"132","PLAZ-02":"546","PLAZ-07 Rep Avg.":"421.5","PLAZ-12":"97.5","PLAZ-01":"889","PLAZ-30":"67.3","PLAZ-28":"217","PLAZ-16":"113","PLAZ-03":"651"},{"Metabolite":"GCA","PLAZ-14":"174","PLAZ-32":"105","PLAZ-23":"ND","PLAZ-09":"410","PLAZ-19":"187","PLAZ-26":"2320","PLAZ-04":"41","PLAZ-34":"317","PLAZ-08":"45.6","PLAZ-25":"114","PLAZ-13":"ND","PLAZ-06 Rep Avg.":"183","PLAZ-29":"226","PLAZ-11":"42.1","PLAZ-05":"60.6","PLAZ-10":"18","PLAZ-21":"ND","PLAZ-35":"113","PLAZ-33":"22.6","PLAZ-31":"213","PLAZ-20":"8.75","PLAZ-24":"66.8","PLAZ-36":"1430","PLAZ-22":"730","PLAZ-18":"39.6","PLAZ-15":"562","PLAZ-17":"ND","PLAZ-27":"53.6","PLAZ-02":"368","PLAZ-07 Rep Avg.":"1345","PLAZ-12":"35.8","PLAZ-01":"184","PLAZ-30":"ND","PLAZ-28":"38.6","PLAZ-16":"6.73","PLAZ-03":"519"},{"Metabolite":"GCDCA","PLAZ-14":"404","PLAZ-32":"250","PLAZ-23":"24.8","PLAZ-09":"604","PLAZ-19":"463","PLAZ-26":"1540","PLAZ-04":"194","PLAZ-34":"928","PLAZ-08":"327","PLAZ-25":"356","PLAZ-13":"430","PLAZ-06 Rep Avg.":"498","PLAZ-29":"442","PLAZ-11":"163","PLAZ-05":"333","PLAZ-10":"107","PLAZ-21":"27.5","PLAZ-35":"140","PLAZ-33":"98.9","PLAZ-31":"338","PLAZ-20":"103","PLAZ-24":"184","PLAZ-36":"1370","PLAZ-22":"842","PLAZ-18":"416","PLAZ-15":"1600","PLAZ-17":"90.8","PLAZ-27":"435","PLAZ-02":"2660","PLAZ-07 Rep Avg.":"1840","PLAZ-12":"98.7","PLAZ-01":"442","PLAZ-30":"79.5","PLAZ-28":"312","PLAZ-16":"127","PLAZ-03":"935"},{"Metabolite":"DCA","PLAZ-14":"197","PLAZ-32":"598","PLAZ-23":"253","PLAZ-09":"162","PLAZ-19":"274","PLAZ-26":"875","PLAZ-04":"121","PLAZ-34":"250","PLAZ-08":"594","PLAZ-25":"328","PLAZ-13":"1210","PLAZ-06 Rep Avg.":"1375","PLAZ-29":"755","PLAZ-11":"1490","PLAZ-05":"679","PLAZ-10":"375","PLAZ-21":"1130","PLAZ-35":"1130","PLAZ-33":"256","PLAZ-31":"970","PLAZ-20":"858","PLAZ-24":"291","PLAZ-36":"2860","PLAZ-22":"677","PLAZ-18":"241","PLAZ-15":"678","PLAZ-17":"1470","PLAZ-27":"1030","PLAZ-02":"1150","PLAZ-07 Rep Avg.":"1620","PLAZ-12":"363","PLAZ-01":"171","PLAZ-30":"248","PLAZ-28":"172","PLAZ-16":"651","PLAZ-03":"537"},{"Metabolite":"LCA","PLAZ-14":"230","PLAZ-32":"299","PLAZ-23":"314","PLAZ-09":"197","PLAZ-19":"748","PLAZ-26":"417","PLAZ-04":"154","PLAZ-34":"167","PLAZ-08":"718","PLAZ-25":"296","PLAZ-13":"1110","PLAZ-06 Rep Avg.":"855.5","PLAZ-29":"523","PLAZ-11":"747","PLAZ-05":"743","PLAZ-10":"468","PLAZ-21":"1690","PLAZ-35":"758","PLAZ-33":"474","PLAZ-31":"729","PLAZ-20":"599","PLAZ-24":"366","PLAZ-36":"1430","PLAZ-22":"449","PLAZ-18":"396","PLAZ-15":"293","PLAZ-17":"963","PLAZ-27":"369","PLAZ-02":"1090","PLAZ-07 Rep Avg.":"556","PLAZ-12":"416","PLAZ-01":"278","PLAZ-30":"346","PLAZ-28":"215","PLAZ-16":"1010","PLAZ-03":"385"},{"Metabolite":"TDCA","PLAZ-14":"313","PLAZ-32":"161","PLAZ-23":"21.8","PLAZ-09":"358","PLAZ-19":"25.5","PLAZ-26":"4880","PLAZ-04":"49.1","PLAZ-34":"205","PLAZ-08":"37.1","PLAZ-25":"89.2","PLAZ-13":"12.1","PLAZ-06 Rep Avg.":"233","PLAZ-29":"410","PLAZ-11":"206","PLAZ-05":"420","PLAZ-10":"39.5","PLAZ-21":"15.4","PLAZ-35":"678","PLAZ-33":"35.9","PLAZ-31":"341","PLAZ-20":"99.6","PLAZ-24":"411","PLAZ-36":"717","PLAZ-22":"2060","PLAZ-18":"55.2","PLAZ-15":"631","PLAZ-17":"33.2","PLAZ-27":"186","PLAZ-02":"358","PLAZ-07 Rep Avg.":"1990","PLAZ-12":"63","PLAZ-01":"457","PLAZ-30":"56.1","PLAZ-28":"340","PLAZ-16":"97.6","PLAZ-03":"2680"},{"Metabolite":"GDCA","PLAZ-14":"176","PLAZ-32":"53.6","PLAZ-23":"22","PLAZ-09":"289","PLAZ-19":"56.6","PLAZ-26":"1910","PLAZ-04":"70.8","PLAZ-34":"416","PLAZ-08":"224","PLAZ-25":"162","PLAZ-13":"327","PLAZ-06 Rep Avg.":"888","PLAZ-29":"199","PLAZ-11":"104","PLAZ-05":"354","PLAZ-10":"86.4","PLAZ-21":"67.2","PLAZ-35":"437","PLAZ-33":"19.8","PLAZ-31":"160","PLAZ-20":"63.4","PLAZ-24":"125","PLAZ-36":"335","PLAZ-22":"1710","PLAZ-18":"177","PLAZ-15":"624","PLAZ-17":"56.5","PLAZ-27":"349","PLAZ-02":"2210","PLAZ-07 Rep Avg.":"6295","PLAZ-12":"58.1","PLAZ-01":"117","PLAZ-30":"33.3","PLAZ-28":"327","PLAZ-16":"86.2","PLAZ-03":"2600"},{"Metabolite":"GLCA","PLAZ-14":"143","PLAZ-32":"51.4","PLAZ-23":"115","PLAZ-09":"158","PLAZ-19":"79.7","PLAZ-26":"267","PLAZ-04":"52.1","PLAZ-34":"158","PLAZ-08":"250","PLAZ-25":"139","PLAZ-13":"618","PLAZ-06 Rep Avg.":"376.5","PLAZ-29":"108","PLAZ-11":"127","PLAZ-05":"288","PLAZ-10":"416","PLAZ-21":"386","PLAZ-35":"185","PLAZ-33":"130","PLAZ-31":"138","PLAZ-20":"51.3","PLAZ-24":"236","PLAZ-36":"262","PLAZ-22":"444","PLAZ-18":"223","PLAZ-15":"104","PLAZ-17":"137","PLAZ-27":"292","PLAZ-02":"659","PLAZ-07 Rep Avg.":"829.5","PLAZ-12":"53.5","PLAZ-01":"69.6","PLAZ-30":"104","PLAZ-28":"245","PLAZ-16":"153","PLAZ-03":"160"},{"Metabolite":"GHDCA","PLAZ-14":"12.7","PLAZ-32":"11.5","PLAZ-23":"12.2","PLAZ-09":"25","PLAZ-19":"12.4","PLAZ-26":"16.4","PLAZ-04":"12.1","PLAZ-34":"13.8","PLAZ-08":"14.5","PLAZ-25":"13","PLAZ-13":"16","PLAZ-06 Rep Avg.":"19.8","PLAZ-29":"13.2","PLAZ-11":"11.9","PLAZ-05":"13.8","PLAZ-10":"12.3","PLAZ-21":"13.9","PLAZ-35":"14.6","PLAZ-33":"11.8","PLAZ-31":"13.8","PLAZ-20":"12.2","PLAZ-24":"14.1","PLAZ-36":"11.4","PLAZ-22":"23.5","PLAZ-18":"13.2","PLAZ-15":"15.7","PLAZ-17":"11.8","PLAZ-27":"16.2","PLAZ-02":"20.6","PLAZ-07 Rep Avg.":"32.2","PLAZ-12":"11.6","PLAZ-01":"12.8","PLAZ-30":"11.9","PLAZ-28":"20.2","PLAZ-16":"12.5","PLAZ-03":"15.7"},{"Metabolite":"UDCA","PLAZ-14":"1.24","PLAZ-32":"39.7","PLAZ-23":"3.05","PLAZ-09":"4.43","PLAZ-19":"12.3","PLAZ-26":"3.63","PLAZ-04":"0.78","PLAZ-34":"3.24","PLAZ-08":"7.59","PLAZ-25":"2.62","PLAZ-13":"29.1","PLAZ-06 Rep Avg.":"7.43","PLAZ-29":"20.2","PLAZ-11":"39.3","PLAZ-05":"1.95","PLAZ-10":"10.6","PLAZ-21":"4.9","PLAZ-35":"1.67","PLAZ-33":"5.25","PLAZ-31":"7.63","PLAZ-20":"2.68","PLAZ-24":"1.28","PLAZ-36":"98.6","PLAZ-22":"0.378","PLAZ-18":"3.07","PLAZ-15":"17.7","PLAZ-17":"36.3","PLAZ-27":"49.3","PLAZ-02":"26.7","PLAZ-07 Rep Avg.":"5.755","PLAZ-12":"7.8","PLAZ-01":"2.78","PLAZ-30":"0.895","PLAZ-28":"3.29","PLAZ-16":"16.3","PLAZ-03":"2.68"},{"Metabolite":"TUDCA","PLAZ-14":"5.13","PLAZ-32":"11.5","PLAZ-23":"2.56","PLAZ-09":"64.8","PLAZ-19":"5.29","PLAZ-26":"39.8","PLAZ-04":"1.68","PLAZ-34":"8.04","PLAZ-08":"2.03","PLAZ-25":"5.81","PLAZ-13":"1.96","PLAZ-06 Rep Avg.":"4.565","PLAZ-29":"6.18","PLAZ-11":"7.07","PLAZ-05":"3.86","PLAZ-10":"3.94","PLAZ-21":"1.91","PLAZ-35":"4.4","PLAZ-33":"2.44","PLAZ-31":"8.97","PLAZ-20":"2.35","PLAZ-24":"24.3","PLAZ-36":"20.3","PLAZ-22":"16.9","PLAZ-18":"1.73","PLAZ-15":"9.74","PLAZ-17":"1.79","PLAZ-27":"7.12","PLAZ-02":"6.24","PLAZ-07 Rep Avg.":"13.6","PLAZ-12":"2.65","PLAZ-01":"16.5","PLAZ-30":"2.09","PLAZ-28":"12.4","PLAZ-16":"3.79","PLAZ-03":"10.8"}],

"Metabolites":[{"metabolite_name":"CA"},{"metabolite_name":"CDCA"},{"metabolite_name":"TCA"},{"metabolite_name":"TCDCA"},{"metabolite_name":"GCA"},{"metabolite_name":"GCDCA"},{"metabolite_name":"DCA"},{"metabolite_name":"LCA"},{"metabolite_name":"TDCA"},{"metabolite_name":"GDCA"},{"metabolite_name":"GLCA"},{"metabolite_name":"GHDCA"},{"metabolite_name":"UDCA"},{"metabolite_name":"TUDCA"}]
}

}