{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000873","ANALYSIS_ID":"AN001479","VERSION":"1","CREATED_ON":"January 3, 2018, 4:03 pm"},

"PROJECT":{"PROJECT_TITLE":"Maternal Hypoxemia and oxidative stress in the fetus, newborn, and adult.","PROJECT_SUMMARY":"Gestational hypoxia presents a significant stress to an unborn fetus that can lead to significant complications related to fetal growth restriction and resulting in diseases in the newborn as well as those manifesting later in life. Recent evidence indicates that inflammation and oxidative stress are contributing factors to hypoxia-related diseases. The Center for Perinatal Biology at Loma Linda University has studied gestational chronic hypoxia in a sheep model for over 20 years to study dysfunction of vascular and nonvascular tissues derived from mothers, fetuses and offspring. In this project we are attempting to use metabolomics to assess metabolic dysregulation in vascular tissues along with markers of oxidative stress and inflammation in the mother and offspring to determine the extent of dysregulation due to chronic hypoxia. Untargeted metabolomics analysis focused on sheep plasma and arteries from the lung, resistance arteries in the brain, uterine arteries, and cultured human myocytes will be used to explore markers of glucose and lipid metabolism disruption. Targeted analyses of oxylipins and endocannabinoids will be used on the same samples to explore markers of oxidative stress and inflammation, which should be increased during hypoxia. This study should delineate pathways and biomarkers that help explain how hypoxia leads to the development of neonatal as well as adult-onset diseases associated with chronic hypoxia that are inter-related with fetal growth restriction.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","FUNDING_SOURCE":"NIH U24DK097154"},

"STUDY":{"STUDY_TITLE":"Maternal Hypoxemia and oxidative stress in the fetus, newborn, and adult. exercise training for peripheral artery disease (part II)","STUDY_TYPE":"Disease model","STUDY_SUMMARY":"Gestational hypoxia presents a significant stress to an unborn fetus that can lead to significant complications related to fetal growth restriction and resulting in diseases in the newborn as well as those manifesting later in life. Recent evidence indicates that inflammation and oxidative stress are contributing factors to hypoxia-related diseases. The Center for Perinatal Biology at Loma Linda University has studied gestational chronic hypoxia in a sheep model for over 20 years to study dysfunction of vascular and nonvascular tissues derived from mothers, fetuses and offspring. In this project we are attempting to use metabolomics to assess metabolic dysregulation in vascular tissues along with markers of oxidative stress and inflammation in the mother and offspring to determine the extent of dysregulation due to chronic hypoxia. Untargeted metabolomics analysis focused on sheep plasma and arteries from the lung, resistance arteries in the brain, uterine arteries, and cultured human myocytes will be used to explore markers of glucose and lipid metabolism disruption. Targeted analyses of oxylipins and endocannabinoids will be used on the same samples to explore markers of oxidative stress and inflammation, which should be increased during hypoxia. This study should delineate pathways and biomarkers that help explain how hypoxia leads to the development of neonatal as well as adult-onset diseases associated with chronic hypoxia that are inter-related with fetal growth restriction.","INSTITUTE":"USDA","DEPARTMENT":"Obesity and metabolism research unit","LABORATORY":"Newman's Lab","LAST_NAME":"Newman","FIRST_NAME":"John","ADDRESS":"430 West Health Sciences Dr. Davis, Ca, 95616","EMAIL":"John.Newman@ars.usda.gov","PHONE":"(530) 752-1009"},

"SUBJECT":{"SUBJECT_TYPE":"Sheep","SUBJECT_SPECIES":"Ovis aries","TAXONOMY_ID":"9940","GENDER":"Both"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"S41",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S42",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S43",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S44",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S45",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S46",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S54",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S55",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S56",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S57",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S58",
"Factors":{"Tissue type":"Adipose","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S47",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S48",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S49",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S50",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S51",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S52",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S53",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S59",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S60",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S61",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S62",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S63",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S64",
"Factors":{"Tissue type":"Adipose","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S36",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S37",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S38",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S39",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S40",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S31",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S32",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S33",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S34",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S35",
"Factors":{"Tissue type":"Carotid","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S26",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S27",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S28",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S29",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S30",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S21",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S23",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S24",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S25",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S107",
"Factors":{"Tissue type":"Carotid","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S89",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S91",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S93",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S104",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S105",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S106",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S71",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S72",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S95",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S79",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S80",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S81",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S82",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S101",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S102",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S83",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S84",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S85",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S87",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S88",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S103",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Fetal"}
},
{
"Subject ID":"-",
"Sample ID":"S74",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S96",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S97",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"New Born"}
},
{
"Subject ID":"-",
"Sample ID":"S65",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S66",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S67",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S68",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S98",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S99",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S100",
"Factors":{"Tissue type":"Pulmonary Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S1",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S2",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S3",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S4",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S5",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S6",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S7",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S8",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S9",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S10",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Normoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S11",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S12",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S13",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S14",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S15",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult non-pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S16",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S17",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S18",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S19",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult pregnant"}
},
{
"Subject ID":"-",
"Sample ID":"S20",
"Factors":{"Tissue type":"Uterine Arteries","Treatment group":"Hypoxic","Life stage":"Adult pregnant"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Part of the routinely flash frozen samples from the Loma Linda University School of Medicine, Center for Perinatal Biology.","SAMPLE_TYPE":"Tissue","TISSUE_CELL_IDENTIFICATION":"Adipose tissue, Carotid, pulmonary arteries, uterine arteries"},

"TREATMENT":{"TREATMENT_SUMMARY":"To induce chronic hypoxia, pregnant and non-pregnant ewes were transported to the White Mountain Research Station that is owned and operated by the University of California. Animals were housed at the Barcroft Research Station (3800 m) for 100+ days prior to having the pregnant or non-pregnant ewes or 2 week old newborn lambs transported back to Loma Linda for study."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"After sample randomization, weighed tissue (around 10mg for adipose and 50mg for arteries) in 2 mL polypropylene Eppendorf tube was enriched with deuterated surrogates in 20µL methanol (Tables S1 and S2 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 400 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol/acetonitrile (v/v) was added, the sample ground with 3mm stainless steel beads (two for adipose tissue and 3 for blood vessel tissue) and agitation for 6 min at 1500RPM. Protein precipitate and debris were removed by centrifugation (10 min, 10,000 RCF, 6 °C). The supernatant was filtered by centrifugation through 1 µm PVDF membranes (Millipore, Billerica, MA) at 6 °C and 4,500 RCF for 3 min. The filtrate was stored in glass vials at -20 °C until UPLC-MS/MS analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Waters Acquity","COLUMN_NAME":"Aquity C18 BEH 1.7µm 100mm x 2.1mm column","FLOW_GRADIENT":"See protocol/methods file","FLOW_RATE":"0.25 mL/min","COLUMN_TEMPERATURE":"60 °C","SOLVENT_A":"0.1% acetic acid","SOLVENT_B":"90% ACN / 10% IPA","INTERNAL_STANDARD":"See protocol/methods file","RETENTION_TIME":"See protocol/methods file","SAMPLE_INJECTION":"5 µL","ANALYTICAL_TIME":"20 min","WEAK_WASH_SOLVENT_NAME":"20% methanol, 10% isopropanol","WEAK_WASH_VOLUME":"600 µL","STRONG_WASH_SOLVENT_NAME":"50:50 Acetonitrile:Methanol","STRONG_WASH_VOLUME":"600 µL"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"ABI Sciex 6500 QTrap","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"Concentration (nM)",

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