{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST000983","ANALYSIS_ID":"AN001609","VERSION":"1","CREATED_ON":"June 27, 2018, 1:40 pm"},

"PROJECT":{"PROJECT_TITLE":"Validating Quantitative Untargeted Lipidomics Across Nine Liquid Chromatography−High-Resolution Mass Spectrometry Platforms","PROJECT_SUMMARY":"Liquid chromatography−mass spectrometry (LC−MS) methods are most often used for untargeted metabolomics and lipidomics. However, methods have not been standardized as accepted “best practice” documents, and reports lack harmonization with respect to quantitative data that enable interstudy comparisons. Researchers use a wide variety of high-resolution mass spectrometers under different operating conditions, and it is unclear if results would yield different biological conclusions depending on the instrument performance. To this end, we used 126 identical human plasma samples and 29 quality control samples from a nutritional intervention study. We investigated lipidomic data acquisitions across nine different MS instruments (1 single TOF, 1 Q/orbital ion trap, and 7 QTOF instruments). Sample preparations, chromatography conditions, and data processing methods were kept identical. Single-point internal standard calibrations were used to estimate absolute concentrations for 307 unique lipids identified by accurate mass, MS/MS spectral match, and retention times. Quantitative results were highly comparable between the LC−MS platforms tested. Using partial least-squares discriminant analysis (PLS-DA) to compare results between platforms, a 92% overlap for the most discriminating lipids based on variable importance in projection (VIP) scores was achieved for all lipids that were detected by at least two instrument platforms. Importantly, even the relative positions of individual samples on the PLS-DA projections were identical. The key for success in harmonizing results was to avoid ion saturation by carefully evaluating linear dynamic ranges using serial dilutions and adjusting the resuspension volume and/or injection volume before running actual study samples.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"5307548258","PUBLICATIONS":"DOI: 10.1021/acs.analchem.7b03404"},

"STUDY":{"STUDY_TITLE":"Validating Quantitative Untargeted Lipidomics Across Nine Liquid Chromatography−High-Resolution Mass Spectrometry Platforms (Part I)","STUDY_SUMMARY":"Liquid chromatography−mass spectrometry (LC−MS) methods are most often used for untargeted metabolomics and lipidomics. However, methods have not been standardized as accepted “best practice” documents, and reports lack harmonization with respect to quantitative data that enable interstudy comparisons. Researchers use a wide variety of high-resolution mass spectrometers under different operating conditions, and it is unclear if results would yield different biological conclusions depending on the instrument performance. To this end, we used 126 identical human plasma samples and 29 quality control samples from a nutritional intervention study. We investigated lipidomic data acquisitions across nine different MS instruments (1 single TOF, 1 Q/orbital ion trap, and 7 QTOF instruments). Sample preparations, chromatography conditions, and data processing methods were kept identical. Single-point internal standard calibrations were used to estimate absolute concentrations for 307 unique lipids identified by accurate mass, MS/MS spectral match, and retention times. Quantitative results were highly comparable between the LC−MS platforms tested. Using partial least-squares discriminant analysis (PLS-DA) to compare results between platforms, a 92% overlap for the most discriminating lipids based on variable importance in projection (VIP) scores was achieved for all lipids that were detected by at least two instrument platforms. Importantly, even the relative positions of individual samples on the PLS-DA projections were identical. The key for success in harmonizing results was to avoid ion saturation by carefully evaluating linear dynamic ranges using serial dilutions and adjusting the resuspension volume and/or injection volume before running actual study samples.","INSTITUTE":"University of California, Davis","DEPARTMENT":"Genome and Biomedical Sciences Facility","LABORATORY":"WCMC Metabolomics Core","LAST_NAME":"Fiehn","FIRST_NAME":"Oliver","ADDRESS":"1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616","EMAIL":"ofiehn@ucdavis.edu","PHONE":"(530) 754-8258","PUBLICATIONS":"DOI: 10.1021/acs.analchem.7b03404"},

"SUBJECT":{"SUBJECT_TYPE":"Human","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"NIST1",
"Sample ID":"GLA_AG3_Lipids_NIST01",
"Factors":{"Collection Time (hours)":"NIST","Phenotype":"NIST"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"NIST"}
},
{
"Subject ID":"NIST2",
"Sample ID":"GLA_AG3_Lipids_NIST02",
"Factors":{"Collection Time (hours)":"NIST","Phenotype":"NIST"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"NIST"}
},
{
"Subject ID":"QC01",
"Sample ID":"GLA_AG3_Lipids_QC01",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC02",
"Sample ID":"GLA_AG3_Lipids_QC02",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC03",
"Sample ID":"GLA_AG3_Lipids_QC03",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC04",
"Sample ID":"GLA_AG3_Lipids_QC04",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC05",
"Sample ID":"GLA_AG3_Lipids_QC05",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC06",
"Sample ID":"GLA_AG3_Lipids_QC06",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC07",
"Sample ID":"GLA_AG3_Lipids_QC07",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC08",
"Sample ID":"GLA_AG3_Lipids_QC08",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC09",
"Sample ID":"GLA_AG3_Lipids_QC09",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC10",
"Sample ID":"GLA_AG3_Lipids_QC10",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC11",
"Sample ID":"GLA_AG3_Lipids_QC11",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC12",
"Sample ID":"GLA_AG3_Lipids_QC12",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC13",
"Sample ID":"GLA_AG3_Lipids_QC13",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC14",
"Sample ID":"GLA_AG3_Lipids_QC14",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC15",
"Sample ID":"GLA_AG3_Lipids_QC15",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"QC16",
"Sample ID":"GLA_AG3_Lipids_QC16",
"Factors":{"Collection Time (hours)":"QC","Phenotype":"QC"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"QC"}
},
{
"Subject ID":"Rep1",
"Sample ID":"GLA_AG3_Lipids_Rep1",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep10",
"Sample ID":"GLA_AG3_Lipids_Rep10",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep2",
"Sample ID":"GLA_AG3_Lipids_Rep2",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep3",
"Sample ID":"GLA_AG3_Lipids_Rep3",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep4",
"Sample ID":"GLA_AG3_Lipids_Rep4",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep5",
"Sample ID":"GLA_AG3_Lipids_Rep5",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep6",
"Sample ID":"GLA_AG3_Lipids_Rep6",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep7",
"Sample ID":"GLA_AG3_Lipids_Rep7",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep8",
"Sample ID":"GLA_AG3_Lipids_Rep8",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"Rep9",
"Sample ID":"GLA_AG3_Lipids_Rep9",
"Factors":{"Collection Time (hours)":"Rep","Phenotype":"Rep"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"Rep"}
},
{
"Subject ID":"1000aC1",
"Sample ID":"GLA_AG3_Lipids_SO001_SA042_1000aC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1000aC2",
"Sample ID":"GLA_AG3_Lipids_SO002_SA044_1000aC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1000aC3",
"Sample ID":"GLA_AG3_Lipids_SO003_SA011_1000aC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1000bG1",
"Sample ID":"GLA_AG3_Lipids_SO004_SA051_1000bG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1000bG2",
"Sample ID":"GLA_AG3_Lipids_SO005_SA002_1000bG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1000bG3",
"Sample ID":"GLA_AG3_Lipids_SO006_SA030_1000bG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1000cC1",
"Sample ID":"GLA_AG3_Lipids_SO007_SA037_1000cC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1000cC2",
"Sample ID":"GLA_AG3_Lipids_SO008_SA020_1000cC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1000cC3",
"Sample ID":"GLA_AG3_Lipids_SO009_SA049_1000cC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1000dG1",
"Sample ID":"GLA_AG3_Lipids_SO010_SA038_1000dG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1000dG2",
"Sample ID":"GLA_AG3_Lipids_SO011_SA041_1000dG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1000dG3",
"Sample ID":"GLA_AG3_Lipids_SO012_SA023_1000dG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1000eC1",
"Sample ID":"GLA_AG3_Lipids_SO013_SA007_1000eC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1000eC2",
"Sample ID":"GLA_AG3_Lipids_SO014_SA035_1000eC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1000eC3",
"Sample ID":"GLA_AG3_Lipids_SO015_SA004_1000eC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1000fG1",
"Sample ID":"GLA_AG3_Lipids_SO016_SA040_1000fG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1000fG2",
"Sample ID":"GLA_AG3_Lipids_SO017_SA018_1000fG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1000fG3",
"Sample ID":"GLA_AG3_Lipids_SO018_SA022_1000fG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1001aG1",
"Sample ID":"GLA_AG3_Lipids_SO019_SA014_1001aG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1001aG2",
"Sample ID":"GLA_AG3_Lipids_SO020_SA021_1001aG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1001aG3",
"Sample ID":"GLA_AG3_Lipids_SO021_SA034_1001aG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1001bC1",
"Sample ID":"GLA_AG3_Lipids_SO022_SA024_1001bC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1001bC2",
"Sample ID":"GLA_AG3_Lipids_SO023_SA012_1001bC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1001bC3",
"Sample ID":"GLA_AG3_Lipids_SO024_SA016_1001bC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1001dC1",
"Sample ID":"GLA_AG3_Lipids_SO025_SA036_1001dC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1001dC2",
"Sample ID":"GLA_AG3_Lipids_SO026_SA019_1001dC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1001dC3",
"Sample ID":"GLA_AG3_Lipids_SO027_SA001_1001dC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1001eG1",
"Sample ID":"GLA_AG3_Lipids_SO028_SA009_1001eG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1001eG2",
"Sample ID":"GLA_AG3_Lipids_SO029_SA053_1001eG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1001eG3",
"Sample ID":"GLA_AG3_Lipids_SO030_SA050_1001eG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1001fC1",
"Sample ID":"GLA_AG3_Lipids_SO031_SA043_1001fC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1001fC2",
"Sample ID":"GLA_AG3_Lipids_SO032_SA017_1001fC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1001fC3",
"Sample ID":"GLA_AG3_Lipids_SO033_SA045_1001fC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1001gG1",
"Sample ID":"GLA_AG3_Lipids_SO034_SA013_1001gG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1001gG2",
"Sample ID":"GLA_AG3_Lipids_SO035_SA047_1001gG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1001gG3",
"Sample ID":"GLA_AG3_Lipids_SO036_SA015_1001gG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002aG1",
"Sample ID":"GLA_AG3_Lipids_SO037_SA048_1002aG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1002aG2",
"Sample ID":"GLA_AG3_Lipids_SO038_SA008_1002aG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002aG3",
"Sample ID":"GLA_AG3_Lipids_SO039_SA046_1002aG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002bC1",
"Sample ID":"GLA_AG3_Lipids_SO040_SA029_1002bC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1002bC2",
"Sample ID":"GLA_AG3_Lipids_SO041_SA052_1002bC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1002bC3",
"Sample ID":"GLA_AG3_Lipids_SO042_SA031_1002bC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1002cG1",
"Sample ID":"GLA_AG3_Lipids_SO043_SA003_1002cG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1002cG2",
"Sample ID":"GLA_AG3_Lipids_SO044_SA025_1002cG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002cG3",
"Sample ID":"GLA_AG3_Lipids_SO045_SA127_1002cG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002dC1",
"Sample ID":"GLA_AG3_Lipids_SO046_SA005_1002dC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1002dC2",
"Sample ID":"GLA_AG3_Lipids_SO047_SA006_1002dC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1002dC3",
"Sample ID":"GLA_AG3_Lipids_SO048_SA128_1002dC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1002eG1",
"Sample ID":"GLA_AG3_Lipids_SO049_SA032_1002eG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1002eG2",
"Sample ID":"GLA_AG3_Lipids_SO050_SA027_1002eG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002eG3",
"Sample ID":"GLA_AG3_Lipids_SO051_SA054_1002eG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1002fC1",
"Sample ID":"GLA_AG3_Lipids_SO052_SA033_1002fC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1002fC2",
"Sample ID":"GLA_AG3_Lipids_SO053_SA028_1002fC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1002fC3",
"Sample ID":"GLA_AG3_Lipids_SO054_SA039_1002fC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003aC1",
"Sample ID":"GLA_AG3_Lipids_SO055_SA078_1003aC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1003aC2",
"Sample ID":"GLA_AG3_Lipids_SO056_SA057_1003aC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003aC3",
"Sample ID":"GLA_AG3_Lipids_SO057_SA071_1003aC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003bG1",
"Sample ID":"GLA_AG3_Lipids_SO058_SA099_1003bG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1003bG2",
"Sample ID":"GLA_AG3_Lipids_SO059_SA060_1003bG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1003bG3",
"Sample ID":"GLA_AG3_Lipids_SO060_SA072_1003bG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1003cC1",
"Sample ID":"GLA_AG3_Lipids_SO061_SA074_1003cC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1003cC2",
"Sample ID":"GLA_AG3_Lipids_SO062_SA055_1003cC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003cC3",
"Sample ID":"GLA_AG3_Lipids_SO063_SA083_1003cC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003dG1",
"Sample ID":"GLA_AG3_Lipids_SO064_SA064_1003dG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1003dG2",
"Sample ID":"GLA_AG3_Lipids_SO065_SA062_1003dG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1003dG3",
"Sample ID":"GLA_AG3_Lipids_SO066_SA090_1003dG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1003eC1",
"Sample ID":"GLA_AG3_Lipids_SO067_SA098_1003eC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1003eC2",
"Sample ID":"GLA_AG3_Lipids_SO068_SA094_1003eC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003eC3",
"Sample ID":"GLA_AG3_Lipids_SO069_SA068_1003eC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1003fG1",
"Sample ID":"GLA_AG3_Lipids_SO070_SA084_1003fG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1003fG2",
"Sample ID":"GLA_AG3_Lipids_SO071_SA097_1003fG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1003fG3",
"Sample ID":"GLA_AG3_Lipids_SO072_SA092_1003fG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1005aG1",
"Sample ID":"GLA_AG3_Lipids_SO073_SA066_1005aG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1005aG2",
"Sample ID":"GLA_AG3_Lipids_SO074_SA089_1005aG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1005aG3",
"Sample ID":"GLA_AG3_Lipids_SO075_SA088_1005aG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1005bC1",
"Sample ID":"GLA_AG3_Lipids_SO076_SA058_1005bC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1005bC2",
"Sample ID":"GLA_AG3_Lipids_SO077_SA082_1005bC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1005bC3",
"Sample ID":"GLA_AG3_Lipids_SO078_SA091_1005bC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1005cG1",
"Sample ID":"GLA_AG3_Lipids_SO079_SA070_1005cG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1005cG2",
"Sample ID":"GLA_AG3_Lipids_SO080_SA079_1005cG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1005cG3",
"Sample ID":"GLA_AG3_Lipids_SO081_SA067_1005cG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1005dC1",
"Sample ID":"GLA_AG3_Lipids_SO082_SA063_1005dC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1005dC2",
"Sample ID":"GLA_AG3_Lipids_SO083_SA069_1005dC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1005dC3",
"Sample ID":"GLA_AG3_Lipids_SO084_SA081_1005dC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1005fC1",
"Sample ID":"GLA_AG3_Lipids_SO085_SA076_1005fC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1005fC2",
"Sample ID":"GLA_AG3_Lipids_SO086_SA095_1005fC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1005fC3",
"Sample ID":"GLA_AG3_Lipids_SO087_SA056_1005fC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1005gG1",
"Sample ID":"GLA_AG3_Lipids_SO088_SA073_1005gG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1005gG2",
"Sample ID":"GLA_AG3_Lipids_SO089_SA080_1005gG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1005gG3",
"Sample ID":"GLA_AG3_Lipids_SO090_SA087_1005gG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006aC1",
"Sample ID":"GLA_AG3_Lipids_SO091_SA061_1006aC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1006aC2",
"Sample ID":"GLA_AG3_Lipids_SO092_SA065_1006aC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1006aC3",
"Sample ID":"GLA_AG3_Lipids_SO093_SA075_1006aC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1006bG1",
"Sample ID":"GLA_AG3_Lipids_SO094_SA085_1006bG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1006bG2",
"Sample ID":"GLA_AG3_Lipids_SO095_SA059_1006bG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006bG3",
"Sample ID":"GLA_AG3_Lipids_SO096_SA093_1006bG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006cC1",
"Sample ID":"GLA_AG3_Lipids_SO097_SA086_1006cC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1006cC2",
"Sample ID":"GLA_AG3_Lipids_SO098_SA077_1006cC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1006cC3",
"Sample ID":"GLA_AG3_Lipids_SO099_SA096_1006cC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1006dG1",
"Sample ID":"GLA_AG3_Lipids_SO100_SA118_1006dG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1006dG2",
"Sample ID":"GLA_AG3_Lipids_SO101_SA104_1006dG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006dG3",
"Sample ID":"GLA_AG3_Lipids_SO102_SA116_1006dG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006eG1",
"Sample ID":"GLA_AG3_Lipids_SO103_SA108_1006eG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1006eG2",
"Sample ID":"GLA_AG3_Lipids_SO104_SA112_1006eG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006eG3",
"Sample ID":"GLA_AG3_Lipids_SO105_SA125_1006eG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006fG1",
"Sample ID":"GLA_AG3_Lipids_SO106_SA123_1006fG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1006fG2",
"Sample ID":"GLA_AG3_Lipids_SO107_SA107_1006fG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1006fG3",
"Sample ID":"GLA_AG3_Lipids_SO108_SA124_1006fG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1007aC1",
"Sample ID":"GLA_AG3_Lipids_SO109_SA122_1007aC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1007aC2",
"Sample ID":"GLA_AG3_Lipids_SO110_SA100_1007aC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1007aC3",
"Sample ID":"GLA_AG3_Lipids_SO111_SA102_1007aC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1007bG1",
"Sample ID":"GLA_AG3_Lipids_SO112_SA103_1007bG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1007bG2",
"Sample ID":"GLA_AG3_Lipids_SO113_SA113_1007bG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1007bG3",
"Sample ID":"GLA_AG3_Lipids_SO114_SA119_1007bG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1007cC1",
"Sample ID":"GLA_AG3_Lipids_SO115_SA106_1007cC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1007cC2",
"Sample ID":"GLA_AG3_Lipids_SO116_SA111_1007cC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1007cC3",
"Sample ID":"GLA_AG3_Lipids_SO117_SA101_1007cC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1007dG1",
"Sample ID":"GLA_AG3_Lipids_SO118_SA110_1007dG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1007dG2",
"Sample ID":"GLA_AG3_Lipids_SO119_SA121_1007dG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1007dG3",
"Sample ID":"GLA_AG3_Lipids_SO120_SA114_1007dG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1007eC1",
"Sample ID":"GLA_AG3_Lipids_SO121_SA105_1007eC1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1007eC2",
"Sample ID":"GLA_AG3_Lipids_SO122_SA120_1007eC2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1007eC3",
"Sample ID":"GLA_AG3_Lipids_SO123_SA115_1007eC3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"CF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"CF"}
},
{
"Subject ID":"1007fG1",
"Sample ID":"GLA_AG3_Lipids_SO124_SA126_1007fG1",
"Factors":{"Collection Time (hours)":"0-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"C"}
},
{
"Subject ID":"1007fG2",
"Sample ID":"GLA_AG3_Lipids_SO125_SA117_1007fG2",
"Factors":{"Collection Time (hours)":"2-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
},
{
"Subject ID":"1007fG3",
"Sample ID":"GLA_AG3_Lipids_SO126_SA109_1007fG3",
"Factors":{"Collection Time (hours)":"4-h","Phenotype":"GF"},
"Additional sample data":{"Organ":"Plasma","Species":"Human","Class-4":"GF"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"We deliberately used samples from a recently published study in order to investigate if statistical and biological conclusions would differ from the published results, depending on the instrumentation used. In a blinded, placebo-controlled, crossover designed study, seven healthy subjects consumed a test meal containing high amounts of gamma-linolenic acid (GLA, 18:3n6) compared to a control meal. Each subject underwent the nutritional test on three separate test days for each test meal, and samples were taken each time over an 8 h period.","COLLECTION_PROTOCOL_FILENAME":"acs_analchem_7b03404.pdf","SAMPLE_TYPE":"Blood (plasma)","COLLECTION_FREQUENCY":"0, 2, and 4 hours"},

"TREATMENT":{"TREATMENT_SUMMARY":"For this study, we used a subset of samples from our recent study focused on nutritional phenotyping in response to a test meal containing gamma-linolenic acid. Briefly, in a single blind, placebo-controlled, crossover design, seven healthy subjects consumed a test meal that consisted of GLA fat (borage oil [denoted GF]) or a control fat (a mixture of corn, safflower, sunflower, and extra-virgin light olive oils [denoted CF]). Compared to the original study, where all subjects were fed on three separate test days for each test meal, a small modification was needed due to sample limitation. Thus, for this study, six subjects were fed on three separate test days for each test meal, while one subject was fed on two separate test days for a control fat meal and four test days for GLA fat (the fourth set was not used in the original study). Plasma samples collected at 0, 2, and 4h in response to the test meals were used for analysis. In total, 126 samples were analyzed out of which 42 were baseline samples (time 0 h), 40 were control fat samples (time 2 and 4 h), and 44 were GLA fat samples (time 2 and 4 h). For quality control, a pool sample consisted of a mixture of nonfasting blood plasma (both control and GLA fat) was used. Also, standard reference material SRM 1950 Metabolites in Frozen Human Plasma (NIST, Gaithersburg, MD) was used.","TREATMENT_PROTOCOL_FILENAME":"acs_analchem_7b03404.pdf"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Extraction of plasma lipids was carried out using a biphasic solvent system of cold methanol, methyl tertbutyl ether (MTBE), and water with some modifications. In more detail, 300 μL of cold methanol containing a mixture of odd chain and deuterated lipid internal standards [LPE(17:1), LPC(17:0), PC(12:0/13:0), PE(17:0/17:0), PG(17:0/17:0), d7-cholesterol, SM(d18:1/17:0), Cer(d18:1/17:0), sphingosine (d17:1), DG(12:0/12:0/0:0), DG(18:1/2:0/0:0), and d5-TG(17:0/17:1/17:0)] was added to a 40 μL blood plasma aliquot in a 2 mL Eppendorf tube and then vortexed (10 s). Then, 1000 μL of cold MTBE containing CE 22:1 (internal standard) was added, followed by vortexing (10 s) and shaking (6 min) at 4 °C. Phase separation was induced by adding 250 μL of LC−MS grade water followed by centrifugation at 14000 rpm for 2 min.","SAMPLEPREP_PROTOCOL_FILENAME":"acs_analchem_7b03404.pdf"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 6530","COLUMN_NAME":"Waters Acquity CSH C18 (100 x 2.1mm, 1.7um) 1.7um Pre-Column","FLOW_GRADIENT":"15% B to 99% B","FLOW_RATE":"0.6 mL/min","COLUMN_TEMPERATURE":"65 C","METHODS_FILENAME":"Data Dictionary Fiehn laboratory_CSH QTOF lipidomics_05-29-2014.pdf","SOLVENT_A":"60:40 Acetonitrile:Water +10mM Ammonium Formate +10mM Formic Acid","SOLVENT_B":"9:1 Isopropanol:Acetonitrile +10mM Ammonium Formate +10mM Formic Acid","COLUMN_PRESSURE":"450-850 bar","INJECTION_TEMPERATURE":"4 C","INTERNAL_STANDARD":"See data dictionary","RETENTION_TIME":"See data dictionary","SAMPLE_INJECTION":"1.67 uL","ANALYTICAL_TIME":"13 min","CAPILLARY_VOLTAGE":"3500 eV","OVEN_TEMPERATURE":"50°C for 1 min, then ramped at 20°C/min to 330°C, held constant for 5 min","TIME_PROGRAM":"15 min","WASHING_BUFFER":"Ethyl Acetate","WEAK_WASH_SOLVENT_NAME":"Isopropanol","STRONG_WASH_SOLVENT_NAME":"Isopropanol","TARGET_SAMPLE_TEMPERATURE":"Autosampler temp 4 C","SAMPLE_LOOP_SIZE":"30 m length x 0.25 mm internal diameter","RANDOMIZATION_ORDER":"Excel generated"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6530 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","CAPILLARY_VOLTAGE":"3500 eV","COLLISION_ENERGY":"25 eV","COLLISION_GAS":"Nitrogen","DRY_GAS_FLOW":"8L/min","DRY_GAS_TEMP":"325 C","FRAGMENT_VOLTAGE":"120 eV","FRAGMENTATION_METHOD":"Auto MS/MS","ION_SOURCE_TEMPERATURE":"325 C","ION_SPRAY_VOLTAGE":"1000","IONIZATION":"Pos","IONIZATION_ENERGY":"70eV","MASS_ACCURACY":"Accurate","REAGENT_GAS":"Nitrogen","SOURCE_TEMPERATURE":"325 C","DATAFORMAT":".d","DESOLVATION_GAS_FLOW":"11 L/min","DESOLVATION_TEMPERATURE":"350 C","NEBULIZER":"35 psig","OCTPOLE_VOLTAGE":"750 eV","RESOLUTION_SETTING":"Extended Dyamic Range","SCAN_RANGE_MOVERZ":"60-1700 Da","SCANNING_CYCLE":"2 Hz","SCANNING_RANGE":"60-1700 Da","SKIMMER_VOLTAGE":"65"},

"MS_METABOLITE_DATA":{
"Units":"nanograms (absolute)",

"Data":[{"Metabolite":"1_CE (22:1) [M+NH4]+ 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}

}