{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001004","ANALYSIS_ID":"AN001714","VERSION":"1","CREATED_ON":"December 17, 2018, 10:01 am"},

"PROJECT":{"PROJECT_TITLE":"Denver Asthma Panel Study-CHEAR Ancillary Study","PROJECT_TYPE":"NIH/NIEHS 1U2CES026560-01","PROJECT_SUMMARY":"Urban environments remain a poorly understood toxic environment for children with asthma, where improved exposure characterization and estimation of exposurehealth outcome relationships are clearly needed. The goal of this project is to investigate the interactions between relevant environmental exposures and asthma severity in a year-long longitudinal study of urban children with asthma. Environmental and clinical samples are being collected at 3 seasonal visits. Using these samples, we will measure the effects of multiple relevant exposures (environmental tobacco smoke (ETS), polycyclic aromatic hydrocarbons (PAHs), phthalates, and volatile organic compounds (VOCs)) on biological responses (metabolomics, oxidative stress, inflammatory markers, and endocannabinoids) and asthma outcomes. Our overall hypothesis is that relevant environmental exposures and their interactions drive disease severity in urban children with asthma. We will test this hypothesis by investigating the following aims:  Aim 1: To investigate how environmental exposures (ETS, PAHs, phthalates, and VOCs) and their interactions contribute to asthma severity in urban children.  Aim 2: To determine if environmental exposures in children with asthma are associated with changes in in biological responses (metabolomics, oxidative stress, inflammatory markers, and endocannabinoids).  Aim 3: To determine which biological responses mediate the relationships between environmental exposures and asthma severity.  Aim 4: To compare environmental exposures and biological responses in asthmatic and non-asthmatic children","INSTITUTE":"Emory University","DEPARTMENT":"School of Medicine","LABORATORY":"Clinical Biomarkers Laboratory","LAST_NAME":"Uppal","FIRST_NAME":"Karan","ADDRESS":"615 Michael St. Ste 225, Atlanta, GA, 30322, USA","EMAIL":"kuppal2@emory.edu","PHONE":"(404) 727 5027","FUNDING_SOURCE":"NIEHS ES026560","CONTRIBUTORS":"Andrew Lui (University of Colorado Denver), Tasha Fingerlin ( University of Colorado Denver), Jonathon Thornburg (University of Colorado Denver), and Dean P. Jones (Emory University)"},

"STUDY":{"STUDY_TITLE":"Denver Asthma Panel Study-CHEAR Ancillary Study","STUDY_TYPE":"Untargeted high-resolution mass spectrometry profiling","STUDY_SUMMARY":"Urban environments remain a poorly understood toxic environment for children with asthma, where improved exposure characterization and estimation of exposurehealth outcome relationships are clearly needed. The goal of this project is to investigate the interactions between relevant environmental exposures and asthma severity in a year-long longitudinal study of urban children with asthma. Environmental and clinical samples are being collected at 3 seasonal visits. Using these samples, we will measure the effects of multiple relevant exposures (environmental tobacco smoke (ETS), polycyclic aromatic hydrocarbons (PAHs), phthalates, and volatile organic compounds (VOCs)) on biological responses (metabolomics, oxidative stress, inflammatory markers, and endocannabinoids) and asthma outcomes. Our overall hypothesis is that relevant environmental exposures and their interactions drive disease severity in urban children with asthma. We will test this hypothesis by investigating the following aims:  Aim 1: To investigate how environmental exposures (ETS, PAHs, phthalates, and VOCs) and their interactions contribute to asthma severity in urban children.  Aim 2: To determine if environmental exposures in children with asthma are associated with changes in in biological responses (metabolomics, oxidative stress, inflammatory markers, and endocannabinoids).  Aim 3: To determine which biological responses mediate the relationships between environmental exposures and asthma severity.  Aim 4: To compare environmental exposures and biological responses in asthmatic and non-asthmatic children","INSTITUTE":"Emory University","DEPARTMENT":"School of Medicine","LABORATORY":"Clincal Biomarkers Laboratory","LAST_NAME":"Uppal","FIRST_NAME":"Karan","ADDRESS":"615 Michael St. Ste 225, Atlanta, GA, 30322, USA","EMAIL":"kuppal2@emory.edu","PHONE":"(404) 727 5027","TOTAL_SUBJECTS":"66","STUDY_COMMENTS":"Both CHEAR and Clinical Biomarker Laboratory pooled plasma samples were used for   quality control. Study specific sample pools were not created"},

"SUBJECT":{"SUBJECT_TYPE":"Plasma samples","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"Teens (12 – 17 yr)"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"NIST SRM 19501_1",
"Sample ID":"nist1_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_001,VT_180424_M342_003,VT_180424_M342_005"}
},
{
"Subject ID":"Q-Std Plasma pool",
"Sample ID":"q3June2014_1a_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_007,VT_180424_M342_009,VT_180424_M342_011"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1a_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_013,VT_180424_M342_015,VT_180424_M342_017"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1b_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_019,VT_180424_M342_021,VT_180424_M342_023"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G4M2-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_025,VT_180424_M342_027,VT_180424_M342_029"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FYU9-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_031,VT_180424_M342_033,VT_180424_M342_035"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G9V7-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_037,VT_180424_M342_039,VT_180424_M342_041"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G1G8-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_043,VT_180424_M342_045,VT_180424_M342_047"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G850-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_049,VT_180424_M342_051,VT_180424_M342_053"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G8A4-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_055,VT_180424_M342_057,VT_180424_M342_059"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G108-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_061,VT_180424_M342_063,VT_180424_M342_065"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G306-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_067,VT_180424_M342_069,VT_180424_M342_071"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G9J3-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_073,VT_180424_M342_075,VT_180424_M342_077"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G900-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_079,VT_180424_M342_081,VT_180424_M342_083"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6J6-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_085,VT_180424_M342_087,VT_180424_M342_089"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FYH8-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_091,VT_180424_M342_093,VT_180424_M342_095"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G652-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_097,VT_180424_M342_099,VT_180424_M342_101"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G967-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_103,VT_180424_M342_105,VT_180424_M342_107"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G181-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_109,VT_180424_M342_111,VT_180424_M342_113"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G0G9-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_115,VT_180424_M342_117,VT_180424_M342_119"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1c_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_121,VT_180424_M342_123,VT_180424_M342_125"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1d_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_127,VT_180424_M342_129,VT_180424_M342_131"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FZY9-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_133,VT_180424_M342_135,VT_180424_M342_137"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G5P4-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_139,VT_180424_M342_141,VT_180424_M342_143"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FXC0-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_145,VT_180424_M342_147,VT_180424_M342_149"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6A6-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_151,VT_180424_M342_153,VT_180424_M342_155"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G7U1-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_157,VT_180424_M342_159,VT_180424_M342_161"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G033-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_163,VT_180424_M342_165,VT_180424_M342_167"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G3C5-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_169,VT_180424_M342_171,VT_180424_M342_173"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1GB35-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_175,VT_180424_M342_177,VT_180424_M342_179"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G0N3-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_181,VT_180424_M342_183,VT_180424_M342_185"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G579-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_187,VT_180424_M342_189,VT_180424_M342_191"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G470-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_193,VT_180424_M342_195,VT_180424_M342_197"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FX76-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_199,VT_180424_M342_201,VT_180424_M342_203"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G8Q9-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_205,VT_180424_M342_207,VT_180424_M342_209"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G1P8-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_211,VT_180424_M342_213,VT_180424_M342_215"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G1R4-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_217,VT_180424_M342_219,VT_180424_M342_221"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6T4-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_223,VT_180424_M342_225,VT_180424_M342_227"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FXR7-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_229,VT_180424_M342_231,VT_180424_M342_233"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1e_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_235,VT_180424_M342_237,VT_180424_M342_239"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1f_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_241,VT_180424_M342_243,VT_180424_M342_245"}
},
{
"Subject ID":"Q-Std Plasma pool",
"Sample ID":"q3June2014_1b_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_247,VT_180424_M342_249,VT_180424_M342_251"}
},
{
"Subject ID":"Q-Std Plasma pool",
"Sample ID":"q3June2014_2a_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_001,VT_180425_M342_003,VT_180425_M342_005"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2a_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_007,VT_180425_M342_009,VT_180425_M342_011"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2b_1",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_013,VT_180425_M342_015,VT_180425_M342_017"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G397-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_019,VT_180425_M342_021,VT_180425_M342_023"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G199-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_025,VT_180425_M342_027,VT_180425_M342_029"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G8P1-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_031,VT_180425_M342_033,VT_180425_M342_035"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G9E4-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_037,VT_180425_M342_039,VT_180425_M342_041"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FY00-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_043,VT_180425_M342_045,VT_180425_M342_047"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FYK1-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_049,VT_180425_M342_051,VT_180425_M342_053"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6M0-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_055,VT_180425_M342_057,VT_180425_M342_059"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G2W2-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_061,VT_180425_M342_063,VT_180425_M342_065"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G124-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_067,VT_180425_M342_069,VT_180425_M342_071"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G363-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_073,VT_180425_M342_075,VT_180425_M342_077"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1GAV9-P-00_1",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_079,VT_180425_M342_081,VT_180425_M342_083"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G4F7-P-00_1",
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{
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{
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"Sample ID":"C-1G6T4-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_224,VT_180424_M342_226,VT_180424_M342_228"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FXR7-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_230,VT_180424_M342_232,VT_180424_M342_234"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1e_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_236,VT_180424_M342_238,VT_180424_M342_240"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_1f_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_242,VT_180424_M342_244,VT_180424_M342_246"}
},
{
"Subject ID":"Q-Std Plasma pool",
"Sample ID":"q3June2014_1b_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180424","Batch":"1","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180424_M342_248,VT_180424_M342_250,VT_180424_M342_252"}
},
{
"Subject ID":"Q-Std Plasma pool",
"Sample ID":"q3June2014_2a_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_002,VT_180425_M342_004,VT_180425_M342_006"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2a_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_008,VT_180425_M342_010,VT_180425_M342_012"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2b_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_014,VT_180425_M342_016,VT_180425_M342_018"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G397-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_020,VT_180425_M342_022,VT_180425_M342_024"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G199-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_026,VT_180425_M342_028,VT_180425_M342_030"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G8P1-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_032,VT_180425_M342_034,VT_180425_M342_036"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G9E4-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_038,VT_180425_M342_040,VT_180425_M342_042"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FY00-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_044,VT_180425_M342_046,VT_180425_M342_048"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FYK1-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_050,VT_180425_M342_052,VT_180425_M342_054"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6M0-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_056,VT_180425_M342_058,VT_180425_M342_060"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G2W2-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_062,VT_180425_M342_064,VT_180425_M342_066"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G124-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_068,VT_180425_M342_070,VT_180425_M342_072"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G363-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_074,VT_180425_M342_076,VT_180425_M342_078"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1GAV9-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_080,VT_180425_M342_082,VT_180425_M342_084"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G4F7-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_086,VT_180425_M342_088,VT_180425_M342_090"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6L2-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_092,VT_180425_M342_094,VT_180425_M342_096"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FYC9-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_098,VT_180425_M342_100,VT_180425_M342_102"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G3D2-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_104,VT_180425_M342_106,VT_180425_M342_108"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G1N2-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_110,VT_180425_M342_112,VT_180425_M342_114"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2c_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_116,VT_180425_M342_118,VT_180425_M342_120"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2d_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_122,VT_180425_M342_124,VT_180425_M342_126"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FYY0-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_128,VT_180425_M342_130,VT_180425_M342_132"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G785-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_134,VT_180425_M342_136,VT_180425_M342_138"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1GB19-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_140,VT_180425_M342_142,VT_180425_M342_144"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G405-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_146,VT_180425_M342_148,VT_180425_M342_150"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G678-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_152,VT_180425_M342_154,VT_180425_M342_156"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G9L9-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_158,VT_180425_M342_160,VT_180425_M342_162"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FZL8-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_164,VT_180425_M342_166,VT_180425_M342_168"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G330-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_170,VT_180425_M342_172,VT_180425_M342_174"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G041-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_176,VT_180425_M342_178,VT_180425_M342_180"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G4E9-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_182,VT_180425_M342_184,VT_180425_M342_186"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G7H0-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_188,VT_180425_M342_190,VT_180425_M342_192"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G8X3-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_194,VT_180425_M342_196,VT_180425_M342_198"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FXX3-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_200,VT_180425_M342_202,VT_180425_M342_204"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G5S7-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_206,VT_180425_M342_208,VT_180425_M342_210"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G6V0-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_212,VT_180425_M342_214,VT_180425_M342_216"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1G876-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_218,VT_180425_M342_220,VT_180425_M342_222"}
},
{
"Subject ID":"Plasma Study Sample",
"Sample ID":"C-1FXD7-P-00_2",
"Factors":{"HRM_Sample_Type":"Study"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_224,VT_180425_M342_226,VT_180425_M342_228"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2e_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_230,VT_180425_M342_232,VT_180425_M342_234"}
},
{
"Subject ID":"CHEAR PlasmaRef_20160726",
"Sample ID":"chearplasma_2f_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_236,VT_180425_M342_238,VT_180425_M342_240"}
},
{
"Subject ID":"Q-Std Plasma pool",
"Sample ID":"q3June2014_2b_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_242,VT_180425_M342_244,VT_180425_M342_246"}
},
{
"Subject ID":"NIST SRM 19502_1",
"Sample ID":"nist2_2",
"Factors":{"HRM_Sample_Type":"Quality Control"},
"Additional sample data":{"Date Analyzed":"180425","Batch":"2","CHEAR_Project_ID":"2016-1450","Sample.Matrix":"Plasma","Raw files":"VT_180425_M342_248,VT_180425_M342_250,VT_180425_M342_252"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Blood plasma samples were collected from nonfasting participants in the Denver Asthma Panel Study at enrollment (baseline). Blood samples were collected in tubes containing EDTA and after centrifugation aliquots of plasma were transferred to 1.8 ml freezer tubes and stored in a -70 degrees C freezer.","SAMPLE_TYPE":"Plasma - EDTA","STORAGE_CONDITIONS":"Described in summary"},

"TREATMENT":{"TREATMENT_SUMMARY":"Samples were received frozen in aliquouts of <250uL. Prior to analysis, samples were thawed and prepared for HRM analysis using the standard protocols described in the Sample Preparation section."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile (Sigma Aldrich) containing 2.5 microliters of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, urine was equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000 rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h).","SAMPLEPREP_PROTOCOL_ID":"HRM_SP_082016_01","SAMPLEPREP_PROTOCOL_FILENAME":"EmoryUniversity_HRM_SP_082016_01.pdf","SAMPLEPREP_PROTOCOL_COMMENTS":"Date effective: 30 July 2016","EXTRACTION_METHOD":"2:1 acetonitrile: sample followed by vortexing and centrifugation"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The HILIC column is operated parallel to reverse phase column for simultaneous analytical separation and column flushing through the use of a dual head HPLC pump equipped with 10-port and 6-port switching valves. During operation of HILIC separation method, the MS is operated in positive ion mode and 10 microliters of sample is injected onto the HILIC column while the reverse phase column is flushing with wash solution. Flow rate is maintained at 0.35 mL/min until 1.5 min, increased to 0.4 mL/min at 4 min and held for 1 min. Solvent A is 100% LC-MS grade water, solvent B is 100% LC-MS grade acetonitrile and solvent C is 2% formic acid (v/v) in LC-MS grade water. Initial mobile phase conditions are 22.5% A, 75% B, 2.5% C hold for 1.5 min, with linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min, resulting in a total analytical run time of 5 min. During the flushing phase (reverse phase analytical separation), the HILIC column is equilibrated with a wash solution of 77.5% A, 20% B, 2.5% C.","CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","COLUMN_NAME":"Waters XBridge BEH Amide XP HILIC column. 2.1mm x 50mm x 2.5µm particle size with Thermo Accucore HILIC guard column with Thermo Accucore HILIC guard column","FLOW_GRADIENT":"A= water, B= acetontrile, C= 2% formic acid in water; 22.5% A, 75% B, 2.5% C hold for 1.5 min, linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min","FLOW_RATE":"0.35 mL/min for 1.5 min; linear increase to 0.4 mL/min at 4 min, hold for 1 min","COLUMN_TEMPERATURE":"60C","METHODS_FILENAME":"20160920_posHILIC120kres5min_ESI_c18negwash.meth","SOLVENT_A":"LC-MS grade water","SOLVENT_B":"LC-MS grade acetonitrile","METHODS_ID":"2% formic acid in LC-MS grade water","SAMPLE_INJECTION":"10 uL","ANALYTICAL_TIME":"5 min","SAMPLE_LOOP_SIZE":"15 uL","SAMPLE_SYRINGE_SIZE":"100 uL","CHROMATOGRAPHY_COMMENTS":"Triplicate injections for each chromatography mode"},

"ANALYSIS":{"LABORATORY_NAME":"Clinical Biomarkers Laboratory","OPERATOR_NAME":"Vilinh Tran","DETECTOR_TYPE":"Orbitrap","SOFTWARE_VERSION":"Xcalibur 3.0.63","ACQUISITION_DATE":"4/25/2018","ANALYSIS_PROTOCOL_FILE":"EmoryUniversity_HRM_QEHF-MS_092017_v1.pdf","DATA_FORMAT":"Profile","ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"POSITIVE","ACQUISITION_DATE":"4/25/2018","CAPILLARY_TEMPERATURE":"250C","COLLISION_GAS":"N2","DRY_GAS_FLOW":"45","DRY_GAS_TEMP":"150C","MASS_ACCURACY":"< 3ppm","SPRAY_VOLTAGE":"3500","ACTIVATION_PARAMETER":"5.00E+05","ACTIVATION_TIME":"118ms","INTERFACE_VOLTAGE":"S-Lens RF level= 55","RESOLUTION_SETTING":"120,000","SCANNING_RANGE":"85-1275","MS_RESULTS_FILE":"ST001004_AN001714_Results.txt UNITS:Peak Area"},

"MS_METABOLITE_DATA":{
"Units":"Peak Area",

"Data":[{"Metabolite":"1-Aminocyclopropanecarboxylic 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m/z":"409.2941","rtimes":"26.9","MSIconfidence(1-4)":"1"},{"Metabolite":"Glycocholic acid","chemical_ID":"HMDB00138","Adduct":"M+H","InChiKey":"InChIKey=RFDAIACWWDREDC-MZMBZMQMSA-N","quantified m/z":"466.3161","rtimes":"37.5","MSIconfidence(1-4)":"1"},{"Metabolite":"Biliverdin","chemical_ID":"HMDB01008","Adduct":"M+H","InChiKey":"InChIKey=RCNSAJSGRJSBKK-YKSNQIBWSA-N","quantified m/z":"583.2547","rtimes":"28","MSIconfidence(1-4)":"1"},{"Metabolite":"Bilirubin","chemical_ID":"HMDB00054","Adduct":"M+H","InChiKey":"InChIKey=BPYKTIZUTYGOLE-IFADSCNNSA-N","quantified m/z":"585.2721","rtimes":"26.7","MSIconfidence(1-4)":"1"},{"Metabolite":"Stachyose","chemical_ID":"HMDB03553","Adduct":"M+H","InChiKey":"InChIKey=UQZIYBXSHAGNOE-XNSRJBNMSA-N","quantified m/z":"667.2282","rtimes":"228.5","MSIconfidence(1-4)":"1"},{"Metabolite":"Palmitoylcarnitine","chemical_ID":"HMDB00222","Adduct":"M+H","InChiKey":"InChIKey=XOMRRQXKHMYMOC-OAQYLSRUSA-N","quantified m/z":"400.3416","rtimes":"26","MSIconfidence(1-4)":"1"}]
}

}