{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001019","ANALYSIS_ID":"AN001667","VERSION":"1","CREATED_ON":"July 18, 2018, 2:27 pm"},

"PROJECT":{"PROJECT_TITLE":"Lipidomic profiles of the heart and circulation in response to exercise versus cardiac pathology: A resource of potential biomarkers and drug targets","PROJECT_SUMMARY":"Lipid profiling was performed on hearts and plasma from mice subjected to a physiological stimulus (4 weeks of swim exercise training) or pathological stimulus (4 weeks of pressure overload – transverse aortic constriction; TAC)","INSTITUTE":"Baker Heart and Diabetes Institute","LABORATORY":"Cardiac Hypertrophy","LAST_NAME":"Tham","FIRST_NAME":"Yow Keat","ADDRESS":"75 Commercial Road, Melbourne 3004","EMAIL":"yowkeat.tham@baker.edu.au","PHONE":"+65385321266","FUNDING_SOURCE":"National Health and Medical Research Council"},

"STUDY":{"STUDY_TITLE":"Lipidomic profiling of heart and plasma of mice following swim training versus pressure overload","STUDY_SUMMARY":"Lipid profiling was performed on hearts and plasma from mice subjected to a physiological stimulus (4 weeks of swim exercise training) or pathological stimulus (4 weeks of pressure overload – transverse aortic constriction; TAC)","INSTITUTE":"Baker Heart and Diabetes Institute","LABORATORY":"Cardiac Hypertrophy","LAST_NAME":"Tham","FIRST_NAME":"Yow Keat","ADDRESS":"75 Commercial Rd, Melbourne 3004","EMAIL":"yowkeat.tham@baker.edu.au","PHONE":"+65385321266"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"FVB/N","AGE_OR_AGE_RANGE":"16.5 to 18.5 weeks old","WEIGHT_OR_WEIGHT_RANGE":"25 to 30 grams","GENDER":"Male","ANIMAL_ANIMAL_SUPPLIER":"in house breeding","ANIMAL_FEED":"Standard chow"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"#B10_0014 YKT_11/8/16_#5",
"Factors":{"Tissue":"Heart","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0015 YKT_11/8/16_#6",
"Factors":{"Tissue":"Heart","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0016 YKT_11/8/16_#7",
"Factors":{"Tissue":"Heart","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0017 YKT_11/8/16_#8",
"Factors":{"Tissue":"Heart","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0018 YKT_11/8/16_#9",
"Factors":{"Tissue":"Heart","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0019 YKT_11/8/16_#10",
"Factors":{"Tissue":"Heart","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0022 YKT_11/8/16_#13",
"Factors":{"Tissue":"Heart","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0023 YKT_11/8/16_#14",
"Factors":{"Tissue":"Heart","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0024 YKT_11/8/16_#15",
"Factors":{"Tissue":"Heart","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0025 YKT_11/8/16_#16",
"Factors":{"Tissue":"Heart","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0026 YKT_11/8/16_#17",
"Factors":{"Tissue":"Heart","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0027 YKT_11/8/16_#18",
"Factors":{"Tissue":"Heart","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0030 YKT_11/8/16_#21",
"Factors":{"Tissue":"Heart","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0031 YKT_11/8/16_#22",
"Factors":{"Tissue":"Heart","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0032 YKT_11/8/16_#23",
"Factors":{"Tissue":"Heart","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0033 YKT_11/8/16_#24",
"Factors":{"Tissue":"Heart","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0035 YKT_11/8/16_#25",
"Factors":{"Tissue":"Heart","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0036 YKT_11/8/16_#26",
"Factors":{"Tissue":"Heart","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0039 YKT_11/8/16_#29",
"Factors":{"Tissue":"Heart","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0040 YKT_11/8/16_#30",
"Factors":{"Tissue":"Heart","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0041 YKT_11/8/16_#31",
"Factors":{"Tissue":"Heart","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0042 YKT_11/8/16_#32",
"Factors":{"Tissue":"Heart","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0043 YKT_11/8/16_#33",
"Factors":{"Tissue":"Heart","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0044 YKT_11/8/16_#34",
"Factors":{"Tissue":"Heart","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0047 YKT_11/8/16_#37",
"Factors":{"Tissue":"Heart","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0048 YKT_11/8/16_#38",
"Factors":{"Tissue":"Heart","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0049 YKT_11/8/16_#39",
"Factors":{"Tissue":"Heart","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0050 YKT_11/8/16_#40",
"Factors":{"Tissue":"Heart","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0051 YKT_11/8/16_#41",
"Factors":{"Tissue":"Heart","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0078 YKT_11/8/16_#67",
"Factors":{"Tissue":"Plasma","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0080 YKT_11/8/16_#68",
"Factors":{"Tissue":"Plasma","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0081 YKT_11/8/16_#69",
"Factors":{"Tissue":"Plasma","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0082 YKT_11/8/16_#70",
"Factors":{"Tissue":"Plasma","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0083 YKT_11/8/16_#71",
"Factors":{"Tissue":"Plasma","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0084 YKT_11/8/16_#72",
"Factors":{"Tissue":"Plasma","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0087 YKT_11/8/16_#75",
"Factors":{"Tissue":"Plasma","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0088 YKT_11/8/16_#76",
"Factors":{"Tissue":"Plasma","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0089 YKT_11/8/16_#77",
"Factors":{"Tissue":"Plasma","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0090 YKT_11/8/16_#78",
"Factors":{"Tissue":"Plasma","Intervention":"SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0091 YKT_11/8/16_#79",
"Factors":{"Tissue":"Plasma","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0092 YKT_11/8/16_#80",
"Factors":{"Tissue":"Plasma","Intervention":"NON SWIM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0095 YKT_11/8/16_#83",
"Factors":{"Tissue":"Plasma","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0096 YKT_11/8/16_#84",
"Factors":{"Tissue":"Plasma","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0097 YKT_11/8/16_#85",
"Factors":{"Tissue":"Plasma","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0098 YKT_11/8/16_#86",
"Factors":{"Tissue":"Plasma","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0099 YKT_11/8/16_#87",
"Factors":{"Tissue":"Plasma","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0100 YKT_11/8/16_#88",
"Factors":{"Tissue":"Plasma","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0104 YKT_11/8/16_#91",
"Factors":{"Tissue":"Plasma","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0105 YKT_11/8/16_#92",
"Factors":{"Tissue":"Plasma","Intervention":"TACMOD"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0106 YKT_11/8/16_#93",
"Factors":{"Tissue":"Plasma","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0107 YKT_11/8/16_#94",
"Factors":{"Tissue":"Plasma","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0108 YKT_11/8/16_#95",
"Factors":{"Tissue":"Plasma","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0109 YKT_11/8/16_#96",
"Factors":{"Tissue":"Plasma","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0112 YKT_11/8/16_#99",
"Factors":{"Tissue":"Plasma","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0113 YKT_11/8/16_#100",
"Factors":{"Tissue":"Plasma","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0114 YKT_11/8/16_#101",
"Factors":{"Tissue":"Plasma","Intervention":"TACSEV"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0115 YKT_11/8/16_#102",
"Factors":{"Tissue":"Plasma","Intervention":"SHAM"}
},
{
"Subject ID":"-",
"Sample ID":"#B10_0116 YKT_11/8/16_#103",
"Factors":{"Tissue":"Plasma","Intervention":"SHAM"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"At study endpoint, prior to tissue collection, mice were anesthetized with sodium pentobarbitone (Lethabarb, 80mg/kg). Once mice had reached an adequate level of anesthesia (assessed by testing the pedal reflex), blood was collected via cardiac puncture using a 25 G needle and 1 ml syringe. This procedure was followed by cervical dislocation. Collected blood was stored on ice in K2EDTA tubes (Beckton Dickinson), centrifuged at 4°C, 4000 g for 10 min, and plasma collected into fresh tubes and stored at -80°C.","SAMPLE_TYPE":"Blood (plasma)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"Swim training - Physiological hypertrophy in mice was achieved via swim training twice daily for 4 weeks, as previously described (McMullen et al., 2003). Swim training commenced in adult mice at approximately 3 months of age with 10 min sessions on the first day, with 10 min increments each day until the maximum of 90 min per session was reached. Mice were rested for at least 4 hours between each session, and water temperature was maintained between 30 to 32°C to avoid thermal stress. At the end of every session, mice were individually towel dried to prevent hypothermia. Plasma and hearts were collected 3 h after the last swim session. Transverse aortic constriction - Male mice were subjected to either sham or TAC surgery at approximately 3-3.5 months of age, as previously described (Xu et al., 2008). Two variations of TAC were performed, designated TAC-moderate (TAC-MOD) and TAC-severe (TAC-SEV). Aortic banding performed on the TAC-MOD mice used a blunt 25 G needle (outside diameter 0.5 mm) placed next to the aorta during ligation to obtain a consistent degree of banding (constriction of blood vessel from ~1 mm to 0.5 mm), while a 27 G needle (outside diameter 0.4 mm) was used for the TAC-SEV group (constriction of blood vessel from ~1 mm to 0.4 mm). Sham operated mice were subjected to the same procedure but no ligation was made."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Cardiac tissue samples were homogenized and sonicated in phosphate buffered saline (PBS) (pH 7.4). The Bicinchoninic acid (BCA) assay (Thermo Scientific) was used to determine protein concentration. A mixture of internal standards (10µl) in CHCl3: methanol (1:1) and 200µl of CHCl3/methanol (2:1) were added to each sample (ventricles- 50µg protein in 10µl; plasma- 10µl) before being briefly vortexed. Samples were first mixed (rotary mixer, 10 min), sonicated (water bath, 30 min at room temperature [RT]) then rested at RT (20 min). Samples were centrifuged (16,000 g, 10 min) and the supernatant was dried under a constant stream of nitrogen gas at 40°C. Extracted lipids were resuspended in 50µl H2O saturated butanol then sonicated (10 min), before 50 µl of methanol with 10mM ammonium was added. Resuspended extracts were centrifuged (3,350 g, 5 min), and the supernatant transferred to glass vials with Teflon insert caps and stored at -80°C until analyzed. Lipid extracts were thawed at RT for an hour, then sonicated in a water bath for 15 min at RT before analysis."},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 6490","COLUMN_NAME":"Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Agilent 6490 QQQ","INSTRUMENT_TYPE":"Triple quadrupole","MS_TYPE":"ESI","ION_MODE":"POSITIVE"},

"MS_METABOLITE_DATA":{
"Units":"pmol/umol PC",

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YKT_11/8/16_#103":"21"},{"Metabolite":"AcylCarnitine(13:0)","#B10_0014 YKT_11/8/16_#5":"10","#B10_0015 YKT_11/8/16_#6":"10","#B10_0016 YKT_11/8/16_#7":"8","#B10_0017 YKT_11/8/16_#8":"9","#B10_0018 YKT_11/8/16_#9":"9","#B10_0019 YKT_11/8/16_#10":"12","#B10_0022 YKT_11/8/16_#13":"9","#B10_0023 YKT_11/8/16_#14":"10","#B10_0024 YKT_11/8/16_#15":"12","#B10_0025 YKT_11/8/16_#16":"11","#B10_0026 YKT_11/8/16_#17":"18","#B10_0027 YKT_11/8/16_#18":"6","#B10_0030 YKT_11/8/16_#21":"13","#B10_0031 YKT_11/8/16_#22":"12","#B10_0032 YKT_11/8/16_#23":"11","#B10_0033 YKT_11/8/16_#24":"12","#B10_0035 YKT_11/8/16_#25":"10","#B10_0036 YKT_11/8/16_#26":"9","#B10_0039 YKT_11/8/16_#29":"21","#B10_0040 YKT_11/8/16_#30":"10","#B10_0041 YKT_11/8/16_#31":"10","#B10_0042 YKT_11/8/16_#32":"10","#B10_0043 YKT_11/8/16_#33":"10","#B10_0044 YKT_11/8/16_#34":"7","#B10_0047 YKT_11/8/16_#37":"9","#B10_0048 YKT_11/8/16_#38":"11","#B10_0049 YKT_11/8/16_#39":"9","#B10_0050 YKT_11/8/16_#40":"8","#B10_0051 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Ion":"369.3"},{"Metabolite":"CE(20:1)","Precursor Ion":"696.7","Product Ion":"369.3"},{"Metabolite":"CE(20:2)","Precursor Ion":"694.7","Product Ion":"369.3"},{"Metabolite":"CE(20:3)","Precursor Ion":"692.6","Product Ion":"369.3"},{"Metabolite":"CE(20:4)","Precursor Ion":"690.6","Product Ion":"369.3"},{"Metabolite":"CE(20:5)","Precursor Ion":"688.6","Product Ion":"369.3"},{"Metabolite":"CE(22:0)","Precursor Ion":"726.7","Product Ion":"369.3"},{"Metabolite":"CE(22:1)","Precursor Ion":"724.7","Product Ion":"369.3"},{"Metabolite":"CE(22:4)","Precursor Ion":"718.7","Product Ion":"369.3"},{"Metabolite":"CE(22:5) (a)","Precursor Ion":"716.6","Product Ion":"369.3"},{"Metabolite":"CE(22:5) (b)","Precursor Ion":"716.6","Product Ion":"369.3"},{"Metabolite":"CE(22:6)","Precursor Ion":"714.6","Product Ion":"369.3"},{"Metabolite":"CE(24:0)","Precursor Ion":"754.7","Product Ion":"369.3"},{"Metabolite":"CE(24:1)","Precursor Ion":"752.7","Product Ion":"369.3"},{"Metabolite":"CE(24:4)","Precursor Ion":"746.7","Product Ion":"369.3"},{"Metabolite":"CE(24:5)","Precursor Ion":"744.7","Product Ion":"369.3"},{"Metabolite":"CE(24:6)","Precursor Ion":"742.7","Product Ion":"369.3"},{"Metabolite":"Cer(d16:1/16:0)","Precursor Ion":"510.6","Product Ion":"236.3"},{"Metabolite":"Cer(d16:1/18:0)","Precursor Ion":"538.6","Product Ion":"236.3"},{"Metabolite":"Cer(d16:1/20:0)","Precursor Ion":"566.6","Product Ion":"236.3"},{"Metabolite":"Cer(d16:1/22:0)","Precursor Ion":"594.6","Product Ion":"236.3"},{"Metabolite":"Cer(d16:1/24:0)","Precursor Ion":"622.6","Product Ion":"236.3"},{"Metabolite":"Cer(d16:1/24:1)","Precursor Ion":"620.6","Product Ion":"236.3"},{"Metabolite":"Cer(d17:1/16:0)","Precursor Ion":"524.6","Product Ion":"250.3"},{"Metabolite":"Cer(d17:1/18:0)","Precursor Ion":"552.6","Product Ion":"250.3"},{"Metabolite":"Cer(d17:1/20:0)","Precursor Ion":"580.6","Product Ion":"250.3"},{"Metabolite":"Cer(d17:1/22:0)","Precursor Ion":"608.6","Product Ion":"250.3"},{"Metabolite":"Cer(d17:1/24:0)","Precursor Ion":"636.6","Product Ion":"250.3"},{"Metabolite":"Cer(d17:1/24:1)","Precursor Ion":"634.6","Product Ion":"250.3"},{"Metabolite":"Cer(d18:1/14:0)","Precursor Ion":"510.5","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/16:0)","Precursor Ion":"538.5","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/18:0)","Precursor Ion":"566.6","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/20:0)","Precursor Ion":"594.6","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/22:0)","Precursor Ion":"622.6","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/24:0)","Precursor Ion":"650.6","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/24:1)","Precursor Ion":"648.6","Product Ion":"264.3"},{"Metabolite":"Cer(d18:1/26:0)","Precursor Ion":"678.6","Product Ion":"264.3"},{"Metabolite":"Cer(d18:2/14:0)","Precursor Ion":"508.5","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/16:0)","Precursor Ion":"536.5","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/17:0)","Precursor Ion":"550.5","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/18:0)","Precursor Ion":"564.6","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/20:0)","Precursor Ion":"592.6","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/22:0)","Precursor Ion":"620.6","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/24:0)","Precursor Ion":"648.6","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/24:1)","Precursor Ion":"646.6","Product Ion":"262.3"},{"Metabolite":"Cer(d18:2/26:0)","Precursor Ion":"676.6","Product Ion":"262.3"},{"Metabolite":"Cer(d19:1/16:0)","Precursor Ion":"552.6","Product Ion":"278.3"},{"Metabolite":"Cer(d19:1/18:0)","Precursor Ion":"580.6","Product Ion":"278.3"},{"Metabolite":"Cer(d19:1/20:0)","Precursor Ion":"608.6","Product Ion":"278.3"},{"Metabolite":"Cer(d19:1/22:0)","Precursor Ion":"636.6","Product Ion":"278.3"},{"Metabolite":"Cer(d19:1/24:0)","Precursor Ion":"664.6","Product Ion":"278.3"},{"Metabolite":"Cer(d19:1/24:1)","Precursor Ion":"662.6","Product Ion":"278.3"},{"Metabolite":"Cer(d19:1/26:0)","Precursor Ion":"692.6","Product Ion":"278.3"},{"Metabolite":"Cer(d20:1/18:0)","Precursor Ion":"594.6","Product Ion":"292.3"},{"Metabolite":"Cer(d20:1/22:0)","Precursor Ion":"650.6","Product Ion":"292.3"},{"Metabolite":"Cer(d20:1/24:0)","Precursor Ion":"678.6","Product Ion":"292.3"},{"Metabolite":"Cer(d20:1/24:1)","Precursor Ion":"676.6","Product Ion":"292.3"},{"Metabolite":"Cer(d20:1/26:0)","Precursor Ion":"706.6","Product Ion":"292.3"},{"Metabolite":"COH","Precursor Ion":"369.4","Product Ion":"161.2"},{"Metabolite":"Desmosterol(16:0)","Precursor Ion":"640.8","Product Ion":"367.4"},{"Metabolite":"Desmosterol(18:1)","Precursor Ion":"666.8","Product Ion":"367.4"},{"Metabolite":"Desmosterol(18:2)","Precursor Ion":"664.8","Product Ion":"367.4"},{"Metabolite":"Desmosterol(20:4)","Precursor Ion":"688.8","Product Ion":"367.4"},{"Metabolite":"Desmosterol(20:5)","Precursor Ion":"686.8","Product Ion":"367.4"},{"Metabolite":"Desmosterol(22:6)","Precursor Ion":"712.8","Product Ion":"367.4"},{"Metabolite":"DG(14:0_16:0)","Precursor Ion":"558.5","Product Ion":"313.3"},{"Metabolite":"DG(16:0_16:0)","Precursor Ion":"586.5","Product Ion":"313.2"},{"Metabolite":"DG(16:0_16:1)","Precursor Ion":"584.5","Product Ion":"313.2"},{"Metabolite":"DG(14:0_18:2)","Precursor Ion":"582.5","Product Ion":"285.2"},{"Metabolite":"DG(16:0_18:1)","Precursor Ion":"612.6","Product Ion":"313.3"},{"Metabolite":"DG(16:1_18:1)","Precursor Ion":"610.5","Product Ion":"339.2"},{"Metabolite":"DG(16:0_18:2)","Precursor Ion":"610.5","Product Ion":"313.2"},{"Metabolite":"DG(18:0_18:1)","Precursor Ion":"640.6","Product Ion":"341.3"},{"Metabolite":"DG(18:1_18:1)","Precursor Ion":"638.6","Product Ion":"339.3"},{"Metabolite":"DG(18:0_18:2)","Precursor Ion":"638.6","Product Ion":"341.3"},{"Metabolite":"DG(18:1_18:2)","Precursor Ion":"636.6","Product Ion":"339.3"},{"Metabolite":"DG(18:2_18:2)","Precursor Ion":"634.5","Product Ion":"337.2"},{"Metabolite":"DG(18:1_18:3)","Precursor Ion":"634.5","Product Ion":"339.2"},{"Metabolite":"DG(16:0_20:4)","Precursor Ion":"634.5","Product Ion":"313.2"},{"Metabolite":"DG(18:1_20:3)","Precursor Ion":"662.6","Product Ion":"339.3"},{"Metabolite":"DG(18:0_20:4)","Precursor Ion":"662.6","Product Ion":"341.3"},{"Metabolite":"DG(18:1_20:4)","Precursor Ion":"660.6","Product Ion":"339.3"},{"Metabolite":"DG(16:0_22:5)","Precursor Ion":"660.6","Product Ion":"313.3"},{"Metabolite":"DG(18:2_20:4)","Precursor Ion":"658.5","Product Ion":"337.2"},{"Metabolite":"DG(16:0_22:6)","Precursor Ion":"658.5","Product Ion":"313.2"},{"Metabolite":"Hex2Cer(d18:1/16:0)","Precursor Ion":"862.6","Product Ion":"264.3"},{"Metabolite":"Hex2Cer(d18:1/18:0)","Precursor Ion":"890.7","Product Ion":"264.3"},{"Metabolite":"Hex2Cer(d18:1/20:0)","Precursor Ion":"918.7","Product Ion":"264.3"},{"Metabolite":"Hex2Cer(d18:1/22:0)","Precursor Ion":"946.7","Product Ion":"264.3"},{"Metabolite":"Hex2Cer(d18:1/24:0)","Precursor Ion":"974.8","Product Ion":"264.3"},{"Metabolite":"Hex2Cer(d18:1/24:1)","Precursor Ion":"972.7","Product Ion":"264.3"},{"Metabolite":"Cer(d18:0/16:0)","Precursor Ion":"540.5","Product Ion":"284.3"},{"Metabolite":"Cer(d18:0/18:0)","Precursor Ion":"568.6","Product Ion":"284.3"},{"Metabolite":"Cer(d18:0/20:0)","Precursor Ion":"596.6","Product Ion":"284.3"},{"Metabolite":"Cer(d18:0/22:0)","Precursor Ion":"624.6","Product Ion":"284.3"},{"Metabolite":"Cer(d18:0/24:0)","Precursor Ion":"652.7","Product Ion":"284.3"},{"Metabolite":"Cer(d18:0/24:1)","Precursor Ion":"650.6","Product Ion":"284.3"},{"Metabolite":"GM1(d18:1/16:0)","Precursor Ion":"760.1","Product Ion":"366.2"},{"Metabolite":"GM3(d18:1/16:0)","Precursor Ion":"1153.7","Product Ion":"264.3"},{"Metabolite":"GM3(d18:1/18:0)","Precursor Ion":"1181.8","Product Ion":"264.3"},{"Metabolite":"GM3(d18:1/20:0)","Precursor Ion":"1209.8","Product Ion":"264.3"},{"Metabolite":"GM3(d18:1/22:0)","Precursor Ion":"1237.8","Product Ion":"264.3"},{"Metabolite":"GM3(d18:1/24:0)","Precursor Ion":"1265.8","Product Ion":"264.3"},{"Metabolite":"GM3(d18:1/24:1)","Precursor Ion":"1263.8","Product Ion":"264.3"},{"Metabolite":"LPC(14:0) [sn2]","Precursor Ion":"468.309","Product Ion":"184.1"},{"Metabolite":"LPC(14:0) [sn1]","Precursor Ion":"468.309","Product Ion":"184.1"},{"Metabolite":"LPC(15:0) [sn2]","Precursor Ion":"482.33","Product Ion":"184.1"},{"Metabolite":"LPC(15:0) [sn1]","Precursor Ion":"482.33","Product Ion":"184.1"},{"Metabolite":"LPC(16:0) [sn2]","Precursor Ion":"496.34","Product Ion":"184.1"},{"Metabolite":"LPC(16:0) [sn1]","Precursor Ion":"496.34","Product Ion":"184.1"},{"Metabolite":"LPC(16:1) [sn2]","Precursor Ion":"494.325","Product Ion":"184.1"},{"Metabolite":"LPC(16:1) [sn1]","Precursor Ion":"494.325","Product Ion":"184.1"},{"Metabolite":"LPC(15-MHDA) [sn2]","Precursor Ion":"510.356","Product Ion":"184.1"},{"Metabolite":"LPC(15-MHDA) [sn1] / LPC(17:0) [sn2]","Precursor Ion":"510.356","Product Ion":"184.1"},{"Metabolite":"LPC(15-MHDA) [104_sn1]","Precursor Ion":"510.356","Product Ion":"104.1"},{"Metabolite":"LPC(17:0) [sn1]","Precursor Ion":"510.356","Product Ion":"184.1"},{"Metabolite":"LPC(17:1 ) [sn2] (a)","Precursor Ion":"508.35","Product Ion":"184.1"},{"Metabolite":"LPC(17:1) [sn1] (a) / LPC(17:1) [sn2] (b)","Precursor Ion":"508.35","Product Ion":"184.1"},{"Metabolite":"LPC 17:1(b) [104_sn1]","Precursor Ion":"508.35","Product Ion":"104.1"},{"Metabolite":"LPC(17:1) [sn1] (b)","Precursor Ion":"508.35","Product Ion":"184.1"},{"Metabolite":"LPC(18:0) [sn2]","Precursor Ion":"524.372","Product Ion":"184.1"},{"Metabolite":"LPC(18:0) [sn1]","Precursor Ion":"524.372","Product Ion":"184.1"},{"Metabolite":"LPC(18:1) [sn2]","Precursor Ion":"522.37","Product Ion":"184.1"},{"Metabolite":"LPC(18:1) [sn1]","Precursor Ion":"522.37","Product Ion":"184.1"},{"Metabolite":"LPC(18:2) [sn2]","Precursor Ion":"520.34","Product Ion":"184.1"},{"Metabolite":"LPC(18:2) [sn1]","Precursor Ion":"520.34","Product Ion":"184.1"},{"Metabolite":"LPC(18:3) [sn2] (a)","Precursor Ion":"518.33","Product Ion":"184.1"},{"Metabolite":"LPC(18:3) [sn1] (a)/LPC(18:3) [sn2] (b)","Precursor Ion":"518.33","Product Ion":"184.1"},{"Metabolite":"LPC(18:3) [sn1] (b)","Precursor Ion":"518.33","Product Ion":"184.1"},{"Metabolite":"LPC(20:0) [sn2]","Precursor Ion":"552.403","Product Ion":"184.1"},{"Metabolite":"LPC(20:0) [sn1]","Precursor Ion":"552.403","Product Ion":"184.1"},{"Metabolite":"LPC(20:1) [sn2]","Precursor Ion":"550.387","Product Ion":"184.1"},{"Metabolite":"LPC(20:1) [sn1]","Precursor Ion":"550.387","Product Ion":"184.1"},{"Metabolite":"LPC(20:2) [sn2]","Precursor Ion":"548.372","Product Ion":"184.1"},{"Metabolite":"LPC(20:2) [sn1]","Precursor Ion":"548.372","Product Ion":"184.1"},{"Metabolite":"LPC(20:3) 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Ion":"570.37","Product Ion":"184.1"},{"Metabolite":"LPC(22:5) [sn1] (a)/LPC(22:5) [sn2] (b)","Precursor Ion":"570.37","Product Ion":"184.1"},{"Metabolite":"LPC(22:5) [sn1] (b)","Precursor Ion":"570.37","Product Ion":"184.1"},{"Metabolite":"LPC(22:6) [sn2]","Precursor Ion":"568.34","Product Ion":"184.1"},{"Metabolite":"LPC(22:6) [sn1]","Precursor Ion":"568.34","Product Ion":"184.1"},{"Metabolite":"LPC(24:0) [sn2]","Precursor Ion":"608.5","Product Ion":"184.1"},{"Metabolite":"LPC(24:0) [sn1]","Precursor Ion":"608.5","Product Ion":"184.1"},{"Metabolite":"LPC(26:0) [sn2]","Precursor Ion":"636.497","Product Ion":"184.1"},{"Metabolite":"LPC(26:0) [sn1]","Precursor Ion":"636.497","Product Ion":"184.1"},{"Metabolite":"LPC(P-16:0)","Precursor Ion":"480.3","Product Ion":"104.1"},{"Metabolite":"LPC(P-18:0)","Precursor Ion":"508.3","Product Ion":"104.1"},{"Metabolite":"LPC(P-18:1)","Precursor Ion":"506.3","Product Ion":"104.1"},{"Metabolite":"LPC(P-20:0)","Precursor Ion":"536.3","Product 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}

}