{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001105","ANALYSIS_ID":"AN001798","VERSION":"1","CREATED_ON":"December 4, 2018, 5:05 pm"},

"PROJECT":{"PROJECT_TITLE":"Retinal ganglion cells lipid profiling","PROJECT_SUMMARY":"The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology, Bascom Palmer Eye Institute,","LABORATORY":"Sanjoy K. Bhattacharya","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy","ADDRESS":"900 NW 17th St, Miami, FL 33136","EMAIL":"sbhattacharya@med.miami.edu","PHONE":"+1 (305) 4824103","FUNDING_SOURCE":"NEI/NIH U01EY027257","CONTRIBUTORS":"Kevin (Kyung) Park, Sanjoy K. Bhattacharya, Ana Carolina Ayupe Oliveira Beckedorff, Anna Trzeciecka"},

"STUDY":{"STUDY_TITLE":"Retinal ganglion cells lipid profiling","STUDY_SUMMARY":"The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology, Bascom Palmer Eye Institute","LABORATORY":"Sanjoy K. Bhattacharya","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy","ADDRESS":"1638 NW 10th Ave, Miami, FL 33136","EMAIL":"sbhattacharya@med.miami.edu","PHONE":"+1 (305) 4824103"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"KPF_1",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_2",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_3",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_4",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_5",
"Factors":{"Treatment":"GFP +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_6",
"Factors":{"Treatment":"GFP +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_7",
"Factors":{"Treatment":"WT"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"-","Sorting":"7/23/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_8",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_9",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_10",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_11",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_12",
"Factors":{"Treatment":"GFP +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_13",
"Factors":{"Treatment":"GFP +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_14",
"Factors":{"Treatment":"WT"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_15",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_16",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_17",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_18",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_19",
"Factors":{"Treatment":"GFP +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_20",
"Factors":{"Treatment":"GFP +ONC"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_21",
"Factors":{"Treatment":"WT"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"-","Sorting":"10/5/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_22",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT+","Sorting":"9/24/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_23",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"OPN4 Cre tdT","Cells":"tdT-","Sorting":"9/24/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_24",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_25",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_26",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_27",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_28",
"Factors":{"Treatment":"tdT +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_29",
"Factors":{"Treatment":"tdT +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_30",
"Factors":{"Treatment":"WT"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"-","Sorting":"7/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A1",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A2",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A3",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A4",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A5",
"Factors":{"Treatment":"tdT +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A6",
"Factors":{"Treatment":"tdT +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A7",
"Factors":{"Treatment":"WT"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"-","Sorting":"4/9/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A8",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A9",
"Factors":{"Treatment":"Intact"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A10",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A11",
"Factors":{"Treatment":"CNTF +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A12",
"Factors":{"Treatment":"tdT +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP -","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A13",
"Factors":{"Treatment":"tdT +ONC"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"CFP +","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KP_A14",
"Factors":{"Treatment":"WT"},
"Additional sample data":{"Genotype":"Thy1-CFP","Cells":"-","Sorting":"4/13/2018","Number of cells":"7,000","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_QC1",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Genotype":"-","Cells":"-","Sorting":"-","Number of cells":"-","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_QC2",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Genotype":"-","Cells":"-","Sorting":"-","Number of cells":"-","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_QC3",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Genotype":"-","Cells":"-","Sorting":"-","Number of cells":"-","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_QC4",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Genotype":"-","Cells":"-","Sorting":"-","Number of cells":"-","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_QC5",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Genotype":"-","Cells":"-","Sorting":"-","Number of cells":"-","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"KPF_QC6",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Genotype":"-","Cells":"-","Sorting":"-","Number of cells":"-","ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"As described in attached method file.","SAMPLE_TYPE":"retinal ganglion cells","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"As described in attached method file.","TREATMENT_PROTOCOL_FILENAME":"Methods.docx"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"As described in attached method file.","SAMPLEPREP_PROTOCOL_FILENAME":"Methods.docx","EXTRACT_STORAGE":"-20℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"As described in attached method file.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Accela 600","COLUMN_NAME":"Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo, Waltham, MA)","METHODS_FILENAME":"Methods.docx"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED"},

"MS_METABOLITE_DATA":{
"Units":"main area",

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}