{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001115","ANALYSIS_ID":"AN001811","VERSION":"1","CREATED_ON":"December 18, 2018, 2:45 pm"},

"PROJECT":{"PROJECT_TITLE":"Growth cone-enriched lipidome of embryonic to early postnatal mouse brain","PROJECT_SUMMARY":"A growth cone (GC) is a part of a neuron considered a hub for axon growth, motility and guidance functions. Unraveling the molecular composition of GCs and events through which active GCs transition to terminal synapses is of importance to developmental and regenerative neuroscience research. Here, we present a dataset on the lipid profiling of the growth cone-enriched fractions derived from E18, P0, P3, P6 and P9 C57BL/6J mouse brains. For comparison, we analyzed non-growth cone membranes.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology, Bascom Palmer Eye Institute,","LABORATORY":"Sanjoy K. Bhattacharya","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy","ADDRESS":"1638 NW 10th Ave, Miami, FL 33136","EMAIL":"sbhattacharya@med.miami.edu","PHONE":"+1 (305) 4824103","CONTRIBUTORS":"Sanjoy K. Bhattacharya, Anna M. Trzeciecka"},

"STUDY":{"STUDY_TITLE":"Growth cone-enriched lipidome of embryonic to early postnatal mouse brain","STUDY_SUMMARY":"A growth cone (GC) is a part of a neuron considered a hub for axon growth, motility and guidance functions. Unraveling the molecular composition of GCs and events through which active GCs transition to terminal synapses is of importance to developmental and regenerative neuroscience research. Here, we present a dataset on the lipid profiling of the growth cone-enriched fractions derived from E18, P0, P3, P6 and P9 C57BL/6J mouse brains. For comparison, we analyzed non-growth cone membranes.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology, Bascom Palmer Eye Institute","LABORATORY":"Sanjoy K. Bhattacharya","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy","ADDRESS":"1638 NW 10th Ave, Miami, FL 33136","EMAIL":"sbhattacharya@med.miami.edu","PHONE":"+1 (305) 4824103"},

"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","ANIMAL_ANIMAL_SUPPLIER":"The Jackson Laboratory"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"GC_E18_1",
"Factors":{"Fraction":"GC","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_E18_2",
"Factors":{"Fraction":"GC","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_E18_3",
"Factors":{"Fraction":"GC","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_E18_4",
"Factors":{"Fraction":"GC","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_E18_5",
"Factors":{"Fraction":"GC","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_E18_6",
"Factors":{"Fraction":"GC","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P0_1",
"Factors":{"Fraction":"GC","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P0_2",
"Factors":{"Fraction":"GC","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P0_3",
"Factors":{"Fraction":"GC","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P0_4",
"Factors":{"Fraction":"GC","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P0_5",
"Factors":{"Fraction":"GC","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P0_6",
"Factors":{"Fraction":"GC","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P3_1",
"Factors":{"Fraction":"GC","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P3_2",
"Factors":{"Fraction":"GC","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P3_3",
"Factors":{"Fraction":"GC","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P3_4",
"Factors":{"Fraction":"GC","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P3_5",
"Factors":{"Fraction":"GC","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P3_6",
"Factors":{"Fraction":"GC","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P6_1",
"Factors":{"Fraction":"GC","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P6_2",
"Factors":{"Fraction":"GC","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P6_3",
"Factors":{"Fraction":"GC","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P6_4",
"Factors":{"Fraction":"GC","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P6_5",
"Factors":{"Fraction":"GC","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P6_6",
"Factors":{"Fraction":"GC","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P9_1",
"Factors":{"Fraction":"GC","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P9_2",
"Factors":{"Fraction":"GC","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P9_3",
"Factors":{"Fraction":"GC","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P9_4",
"Factors":{"Fraction":"GC","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P9_5",
"Factors":{"Fraction":"GC","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"GC_P9_6",
"Factors":{"Fraction":"GC","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_E18_1",
"Factors":{"Fraction":"M","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_E18_2",
"Factors":{"Fraction":"M","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_E18_3",
"Factors":{"Fraction":"M","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_E18_4",
"Factors":{"Fraction":"M","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_E18_5",
"Factors":{"Fraction":"M","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_E18_6",
"Factors":{"Fraction":"M","Age":"E18"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P0_1",
"Factors":{"Fraction":"M","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P0_2",
"Factors":{"Fraction":"M","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P0_3",
"Factors":{"Fraction":"M","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P0_4",
"Factors":{"Fraction":"M","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P0_5",
"Factors":{"Fraction":"M","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P0_6",
"Factors":{"Fraction":"M","Age":"P0"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P3_1",
"Factors":{"Fraction":"M","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P3_2",
"Factors":{"Fraction":"M","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P3_3",
"Factors":{"Fraction":"M","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P3_4",
"Factors":{"Fraction":"M","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P3_5",
"Factors":{"Fraction":"M","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P3_6",
"Factors":{"Fraction":"M","Age":"P3"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P6_1",
"Factors":{"Fraction":"M","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P6_2",
"Factors":{"Fraction":"M","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P6_3",
"Factors":{"Fraction":"M","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P6_4",
"Factors":{"Fraction":"M","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P6_5",
"Factors":{"Fraction":"M","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P6_6",
"Factors":{"Fraction":"M","Age":"P6"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P9_1",
"Factors":{"Fraction":"M","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P9_2",
"Factors":{"Fraction":"M","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P9_3",
"Factors":{"Fraction":"M","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P9_4",
"Factors":{"Fraction":"M","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P9_5",
"Factors":{"Fraction":"M","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
},
{
"Subject ID":"-",
"Sample ID":"M_P9_6",
"Factors":{"Fraction":"M","Age":"P9"},
"Additional sample data":{"ESI mode":"positive/negative","NCE":"15/30/45/60/75/90"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Growth cones (GC) were enriched by sucrose-gradient centrifugation from embryonic and postnatal C57BL/6J mouse brains. Non-GC membranes (M) were collected for comparison.","SAMPLE_TYPE":"Brain","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"As described in the method."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"As described in the method.","EXTRACT_STORAGE":"-20℃"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Reversed phase chromatographic separation utilized Accela Autosampler (Thermo), Accela 600 pump (Thermo) and Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo). The column temperature was maintained at 30˚C and tray temperature at 20˚C. Solvent A was composed of 10 mM ammonium acetate (LC-MS grade) in 60:40 methanol:water (LC-MS grade) with 0.2% formic acid (FA; LC-MS grade). Solvent B was composed of 10 mM ammonium acetate with 60:40 methanol:chloroform with 0.2% FA. The flow rate was 260 µL/min and injection volume was 10 µL. The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min.","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Thermo Accela 600","COLUMN_NAME":"Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo)"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"MS_COMMENTS":"-","INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED"},

"MS_METABOLITE_DATA":{
"Units":"main area",

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