{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001135","ANALYSIS_ID":"AN001860","VERSION":"1","CREATED_ON":"February 13, 2019, 5:53 pm"},

"PROJECT":{"PROJECT_TITLE":"Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler","PROJECT_SUMMARY":"Inducing mitochondrial uncoupling (mUncoupling) is an attractive therapeutic strategy for treating metabolic diseases because it leads to calorie-wasting by reducing the efficiency of oxidative phosphorylation (OXPHOS) in mitochondria. Here we report a safe mUncoupler, OPC-163493, which has unique pharmacokinetic characteristics. OPC-163493 shows a good bioavailability upon oral administration and primarily distributed to specific organs: the liver and kidneys, avoiding systemic toxicities. It exhibitsinsulin-independent antidiabetic effects in multiple animal models of type I and type II diabetes and antisteatotic effects in fatty liver models. These beneficial effects can be explained by the improvement of glucose metabolism and enhancement of energy expenditure by OPC-163493 in the liver. Moreover, OPC-163493 treatment lowered blood pressure, extended survival, and improved renal function in the rat model of stroke/hypertension, possibly by enhancing NO bioavailability in blood vessels and reducing mitochondrial ROS production. OPC-163493 is a liver-localized/targeted mUncoupler that ameliorates various complications of diabetes.","INSTITUTE":"Otsuka Pharmacetical Co., Ltd.","LAST_NAME":"Kanemoto","FIRST_NAME":"Naohide","ADDRESS":"463-10 Kagasuno Kawauchi-cho, Tokushima, Tokusima, 770-0865, Japan","EMAIL":"Kanemoto.Naohide@otsuka.jp","PHONE":"+81-88-665-2126"},

"STUDY":{"STUDY_TITLE":"Different dose exposure of OPC-163493 on HepG2 cells (part-I)","STUDY_TYPE":"Compound dosage test","STUDY_SUMMARY":"Metabolomics analysis were on 8 samples of HepG2 cells that were treated with compound OPC-163493 (DMSO control, 1, 3, or 10µM; each n=2) exposure for 30 min.","INSTITUTE":"Otsuka Pharmaceuticals","LAST_NAME":"Kanemoto","FIRST_NAME":"Naohide","ADDRESS":"463-10 Kagasuno Kawauchi-cho Tokushima 771-0192, Japan","EMAIL":"Kanemoto.Naohide@otsuka.jp","PHONE":"81-03-6717-1400"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","GENDER":"Not applicable","CELL_STRAIN_DETAILS":"HepG2 cells"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"OPC-0uM-1",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-0uM-5",
"Factors":{"Treatment":"-"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-1uM-2",
"Factors":{"Treatment":"1"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-1uM-6",
"Factors":{"Treatment":"1"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-3uM-3",
"Factors":{"Treatment":"3"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-3uM-7",
"Factors":{"Treatment":"3"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-10uM-4",
"Factors":{"Treatment":"10"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
},
{
"Subject ID":"-",
"Sample ID":"OPC-10uM-8",
"Factors":{"Treatment":"10"},
"Additional sample data":{"Duration (min)":"30","Cell line":"HepG2 cell"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Culture medium of HepG2 cells in 100-mm dish was aspirated and cells were washed twice by 5% mannitol solution, and then processed with sampleprep steps.","SAMPLE_TYPE":"HepG2 cells","STORAGE_CONDITIONS":"Room temperature"},

"TREATMENT":{"TREATMENT_SUMMARY":"HepG2 cells were seeded into a 100mm dish the day before OPC-163493 treatment. OPC-163493 treatments (DMSO control, 1, 3, or 10uM, each n=2) were performed for 30min in FBS-free DMEM with high glucose (25mM).","TREATMENT_DOSE":"0uM, 1uM, 3uM, 10uM","TREATMENT_DOSEDURATION":"30 min","CELL_MEDIA":"MEM with Earle`s salts, L-Glutamine and Non-Essiontial Amino Acids, 10% fetal bovine serum, and 1mM sodium pyruvate solution"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Culture medium of HepG2 cells in 100-mm dish was aspirated and cells were washed twice by 5% mannitol solution, and then the cells were treated with methanol. The cell extract was treated with Mili-Q water containing internal standard and filtered with 5-kDa cutoff filter. The filtrate was centrifugally concentrated and re-suspended in 50 µL of Milli-Q water.","PROCESSING_STORAGE_CONDITIONS":"Room temperature","EXTRACT_STORAGE":"-80℃","SAMPLE_RESUSPENSION":"50 uL Mili-Q"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"capillary electrophoresis was connected with time-of-flight mass spectrometry (CE-TOFMS) for cation analysis and tandem mass spectrometry (CE-MS/MS) for anion.","CHROMATOGRAPHY_TYPE":"CE","INSTRUMENT_NAME":"Agilent 7100 CE","COLUMN_NAME":"Fused silica capillary, i.d. 50 µm × 80 cm"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6210 TOF","INSTRUMENT_TYPE":"Other","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (Keio University, Tsuruoka, Japan) in order to obtain peak information including m/z, migration time for CE-TOFMS measurement (MT) and peak area. Signal peaks corresponding to isotopomers, adduct ions, and other product ions of known metabolites were excluded, and remaining peaks were annotated with putative metabolites from the HMT metabolite database based on their MTs and m/z values determined by TOFMS. The tolerance range for the peak annotation was configured at ±0.5 min for MT and ±10 ppm for m/z. In addition, peak areas were normalized against those of the internal standards and then the resultant relative area values were further normalized by sample amount."},

"MS_METABOLITE_DATA":{
"Units":"Concentration (pmol/1000000 cells)",

"Data":[{"Metabolite":"Adenine","OPC-0uM-1":"23.6","OPC-0uM-5":"17.7","OPC-1uM-2":"20.7","OPC-1uM-6":"20.5","OPC-3uM-3":"25.6","OPC-3uM-7":"18.3","OPC-10uM-4":"15.6","OPC-10uM-8":"15.7"},{"Metabolite":"Adenosine","OPC-0uM-1":"9.20","OPC-0uM-5":"10.30","OPC-1uM-2":"10.04","OPC-1uM-6":"9.61","OPC-3uM-3":"11.38","OPC-3uM-7":"9.37","OPC-10uM-4":"10.07","OPC-10uM-8":"10.59"},{"Metabolite":"Alanine","OPC-0uM-1":"12432","OPC-0uM-5":"11711","OPC-1uM-2":"9414","OPC-1uM-6":"10703","OPC-3uM-3":"9154","OPC-3uM-7":"7941","OPC-10uM-4":"4284","OPC-10uM-8":"4301"},{"Metabolite":"Arginine","OPC-0uM-1":"890","OPC-0uM-5":"919","OPC-1uM-2":"870","OPC-1uM-6":"952","OPC-3uM-3":"1059","OPC-3uM-7":"948","OPC-10uM-4":"1052","OPC-10uM-8":"1105"},{"Metabolite":"Argininosuccinic acid","OPC-0uM-1":"32.4","OPC-0uM-5":"27.9","OPC-1uM-2":"29.5","OPC-1uM-6":"27.3","OPC-3uM-3":"30.8","OPC-3uM-7":"27.9","OPC-10uM-4":"20.4","OPC-10uM-8":"17.9"},{"Metabolite":"Asparagine","OPC-0uM-1":"455","OPC-0uM-5":"398","OPC-1uM-2":"394","OPC-1uM-6":"393","OPC-3uM-3":"422","OPC-3uM-7":"332","OPC-10uM-4":"400","OPC-10uM-8":"338"},{"Metabolite":"Aspartic acid","OPC-0uM-1":"11047","OPC-0uM-5":"12559","OPC-1uM-2":"9616","OPC-1uM-6":"11342","OPC-3uM-3":"9440","OPC-3uM-7":"9449","OPC-10uM-4":"4934","OPC-10uM-8":"5886"},{"Metabolite":"Beta-Alanine","OPC-0uM-1":"390","OPC-0uM-5":"370","OPC-1uM-2":"333","OPC-1uM-6":"376","OPC-3uM-3":"402","OPC-3uM-7":"362","OPC-10uM-4":"362","OPC-10uM-8":"340"},{"Metabolite":"Betaine","OPC-0uM-1":"484","OPC-0uM-5":"635","OPC-1uM-2":"528","OPC-1uM-6":"645","OPC-3uM-3":"646","OPC-3uM-7":"632","OPC-10uM-4":"594","OPC-10uM-8":"652"},{"Metabolite":"Betaine aldehyde","OPC-0uM-1":"0.352","OPC-0uM-5":"N.D.","OPC-1uM-2":"0.537","OPC-1uM-6":"0.006","OPC-3uM-3":"0.712","OPC-3uM-7":"N.D.","OPC-10uM-4":"N.D.","OPC-10uM-8":"N.D."},{"Metabolite":"Carnitine","OPC-0uM-1":"599","OPC-0uM-5":"635","OPC-1uM-2":"593","OPC-1uM-6":"647","OPC-3uM-3":"586","OPC-3uM-7":"597","OPC-10uM-4":"548","OPC-10uM-8":"574"},{"Metabolite":"Carnosine","OPC-0uM-1":"9.46","OPC-0uM-5":"9.97","OPC-1uM-2":"8.61","OPC-1uM-6":"9.08","OPC-3uM-3":"9.15","OPC-3uM-7":"9.90","OPC-10uM-4":"9.32","OPC-10uM-8":"9.86"},{"Metabolite":"Choline","OPC-0uM-1":"159","OPC-0uM-5":"197","OPC-1uM-2":"147","OPC-1uM-6":"191","OPC-3uM-3":"193","OPC-3uM-7":"157","OPC-10uM-4":"177","OPC-10uM-8":"204"},{"Metabolite":"cis-3-hydroxyproline","OPC-0uM-1":"81.8","OPC-0uM-5":"92.7","OPC-1uM-2":"84.8","OPC-1uM-6":"86.5","OPC-3uM-3":"88.7","OPC-3uM-7":"94.3","OPC-10uM-4":"90.2","OPC-10uM-8":"91.1"},{"Metabolite":"Citrulline","OPC-0uM-1":"10.03","OPC-0uM-5":"10.57","OPC-1uM-2":"9.77","OPC-1uM-6":"10.28","OPC-3uM-3":"12.35","OPC-3uM-7":"10.82","OPC-10uM-4":"10.88","OPC-10uM-8":"11.14"},{"Metabolite":"Creatine","OPC-0uM-1":"4167","OPC-0uM-5":"4497","OPC-1uM-2":"4223","OPC-1uM-6":"4705","OPC-3uM-3":"5555","OPC-3uM-7":"5035","OPC-10uM-4":"5805","OPC-10uM-8":"6347"},{"Metabolite":"Creatinine","OPC-0uM-1":"5.25","OPC-0uM-5":"5.50","OPC-1uM-2":"4.89","OPC-1uM-6":"7.15","OPC-3uM-3":"7.48","OPC-3uM-7":"6.07","OPC-10uM-4":"6.83","OPC-10uM-8":"6.22"},{"Metabolite":"Cystathionine","OPC-0uM-1":"1791","OPC-0uM-5":"2156","OPC-1uM-2":"1840","OPC-1uM-6":"2177","OPC-3uM-3":"2063","OPC-3uM-7":"2111","OPC-10uM-4":"1941","OPC-10uM-8":"2063"},{"Metabolite":"Cysteine","OPC-0uM-1":"602","OPC-0uM-5":"378","OPC-1uM-2":"359","OPC-1uM-6":"321","OPC-3uM-3":"817","OPC-3uM-7":"325","OPC-10uM-4":"662","OPC-10uM-8":"509"},{"Metabolite":"Gamma-Aminobutyric acid","OPC-0uM-1":"8.14","OPC-0uM-5":"7.77","OPC-1uM-2":"8.84","OPC-1uM-6":"7.50","OPC-3uM-3":"9.47","OPC-3uM-7":"6.84","OPC-10uM-4":"9.76","OPC-10uM-8":"7.86"},{"Metabolite":"Glutamic acid","OPC-0uM-1":"55195","OPC-0uM-5":"63135","OPC-1uM-2":"53295","OPC-1uM-6":"60239","OPC-3uM-3":"61228","OPC-3uM-7":"59639","OPC-10uM-4":"58612","OPC-10uM-8":"62625"},{"Metabolite":"Glutamine","OPC-0uM-1":"54299","OPC-0uM-5":"57601","OPC-1uM-2":"51123","OPC-1uM-6":"57098","OPC-3uM-3":"60057","OPC-3uM-7":"53072","OPC-10uM-4":"48176","OPC-10uM-8":"50866"},{"Metabolite":"Glutathione","OPC-0uM-1":"18710","OPC-0uM-5":"22947","OPC-1uM-2":"14342","OPC-1uM-6":"20757","OPC-3uM-3":"22146","OPC-3uM-7":"18541","OPC-10uM-4":"22934","OPC-10uM-8":"23263"},{"Metabolite":"Glycine","OPC-0uM-1":"25088","OPC-0uM-5":"24691","OPC-1uM-2":"23745","OPC-1uM-6":"25504","OPC-3uM-3":"28075","OPC-3uM-7":"24249","OPC-10uM-4":"24921","OPC-10uM-8":"25217"},{"Metabolite":"Guanosine","OPC-0uM-1":"3.95","OPC-0uM-5":"N.D.","OPC-1uM-2":"N.D.","OPC-1uM-6":"N.D.","OPC-3uM-3":"N.D.","OPC-3uM-7":"N.D.","OPC-10uM-4":"4.17","OPC-10uM-8":"N.D."},{"Metabolite":"Histidine","OPC-0uM-1":"3697","OPC-0uM-5":"3656","OPC-1uM-2":"3350","OPC-1uM-6":"4132","OPC-3uM-3":"4099","OPC-3uM-7":"3821","OPC-10uM-4":"3985","OPC-10uM-8":"4135"},{"Metabolite":"Homocysteine","OPC-0uM-1":"N.D.","OPC-0uM-5":"N.D.","OPC-1uM-2":"N.D.","OPC-1uM-6":"N.D.","OPC-3uM-3":"N.D.","OPC-3uM-7":"N.D.","OPC-10uM-4":"N.D.","OPC-10uM-8":"2.97"},{"Metabolite":"Homoserine","OPC-0uM-1":"13.6","OPC-0uM-5":"12.9","OPC-1uM-2":"13.2","OPC-1uM-6":"15.2","OPC-3uM-3":"N.D.","OPC-3uM-7":"16.2","OPC-10uM-4":"N.D.","OPC-10uM-8":"12.7"},{"Metabolite":"Inosine","OPC-0uM-1":"N.D.","OPC-0uM-5":"N.D.","OPC-1uM-2":"N.D.","OPC-1uM-6":"N.D.","OPC-3uM-3":"N.D.","OPC-3uM-7":"0.903","OPC-10uM-4":"N.D.","OPC-10uM-8":"N.D."},{"Metabolite":"Isoleucine","OPC-0uM-1":"6087","OPC-0uM-5":"6197","OPC-1uM-2":"5737","OPC-1uM-6":"6492","OPC-3uM-3":"7031","OPC-3uM-7":"6292","OPC-10uM-4":"6299","OPC-10uM-8":"6688"},{"Metabolite":"Leucine","OPC-0uM-1":"7514","OPC-0uM-5":"8021","OPC-1uM-2":"6994","OPC-1uM-6":"8279","OPC-3uM-3":"8565","OPC-3uM-7":"7729","OPC-10uM-4":"7793","OPC-10uM-8":"8586"},{"Metabolite":"Lysine","OPC-0uM-1":"2393","OPC-0uM-5":"2603","OPC-1uM-2":"2391","OPC-1uM-6":"2713","OPC-3uM-3":"2762","OPC-3uM-7":"2422","OPC-10uM-4":"2835","OPC-10uM-8":"2929"},{"Metabolite":"Methionine","OPC-0uM-1":"4961","OPC-0uM-5":"5371","OPC-1uM-2":"4642","OPC-1uM-6":"5444","OPC-3uM-3":"5333","OPC-3uM-7":"5415","OPC-10uM-4":"5387","OPC-10uM-8":"5789"},{"Metabolite":"Ornithine","OPC-0uM-1":"76.7","OPC-0uM-5":"93.7","OPC-1uM-2":"74.3","OPC-1uM-6":"91.9","OPC-3uM-3":"65.1","OPC-3uM-7":"70.4","OPC-10uM-4":"47.8","OPC-10uM-8":"38.6"},{"Metabolite":"Oxidized glutathione","OPC-0uM-1":"5703","OPC-0uM-5":"5305","OPC-1uM-2":"6472","OPC-1uM-6":"5842","OPC-3uM-3":"5502","OPC-3uM-7":"5830","OPC-10uM-4":"3560","OPC-10uM-8":"3635"},{"Metabolite":"Phenylalanine","OPC-0uM-1":"4984","OPC-0uM-5":"5168","OPC-1uM-2":"4691","OPC-1uM-6":"5254","OPC-3uM-3":"5549","OPC-3uM-7":"5007","OPC-10uM-4":"5165","OPC-10uM-8":"5413"},{"Metabolite":"Proline","OPC-0uM-1":"8283","OPC-0uM-5":"8465","OPC-1uM-2":"7272","OPC-1uM-6":"7747","OPC-3uM-3":"7335","OPC-3uM-7":"6720","OPC-10uM-4":"4165","OPC-10uM-8":"4201"},{"Metabolite":"Putrescine","OPC-0uM-1":"4.63","OPC-0uM-5":"4.79","OPC-1uM-2":"4.57","OPC-1uM-6":"3.82","OPC-3uM-3":"4.07","OPC-3uM-7":"3.43","OPC-10uM-4":"3.29","OPC-10uM-8":"N.D."},{"Metabolite":"S-Adenosylhomocysteine","OPC-0uM-1":"41.4","OPC-0uM-5":"76.5","OPC-1uM-2":"57.8","OPC-1uM-6":"57.0","OPC-3uM-3":"43.9","OPC-3uM-7":"49.1","OPC-10uM-4":"68.8","OPC-10uM-8":"60.8"},{"Metabolite":"S-Adenosylmethionine","OPC-0uM-1":"976","OPC-0uM-5":"1008","OPC-1uM-2":"870","OPC-1uM-6":"1136","OPC-3uM-3":"1084","OPC-3uM-7":"1105","OPC-10uM-4":"915","OPC-10uM-8":"1097"},{"Metabolite":"Sarcosine","OPC-0uM-1":"N.D.","OPC-0uM-5":"16.35","OPC-1uM-2":"8.72","OPC-1uM-6":"N.D.","OPC-3uM-3":"17.85","OPC-3uM-7":"3.07","OPC-10uM-4":"13.53","OPC-10uM-8":"14.62"},{"Metabolite":"Serine","OPC-0uM-1":"20596","OPC-0uM-5":"21174","OPC-1uM-2":"18361","OPC-1uM-6":"20581","OPC-3uM-3":"21268","OPC-3uM-7":"19113","OPC-10uM-4":"16595","OPC-10uM-8":"16974"},{"Metabolite":"Spermidine","OPC-0uM-1":"8.07","OPC-0uM-5":"8.56","OPC-1uM-2":"8.03","OPC-1uM-6":"8.71","OPC-3uM-3":"8.85","OPC-3uM-7":"9.14","OPC-10uM-4":"5.47","OPC-10uM-8":"7.54"},{"Metabolite":"Spermine","OPC-0uM-1":"40.62","OPC-0uM-5":"35.73","OPC-1uM-2":"33.65","OPC-1uM-6":"47.92","OPC-3uM-3":"37.86","OPC-3uM-7":"37.10","OPC-10uM-4":"9.83","OPC-10uM-8":"28.58"},{"Metabolite":"Threonine","OPC-0uM-1":"31255","OPC-0uM-5":"31006","OPC-1uM-2":"28471","OPC-1uM-6":"31658","OPC-3uM-3":"33751","OPC-3uM-7":"29901","OPC-10uM-4":"27489","OPC-10uM-8":"28305"},{"Metabolite":"Tryptophan","OPC-0uM-1":"580.3","OPC-0uM-5":"613.4","OPC-1uM-2":"541.2","OPC-1uM-6":"635.1","OPC-3uM-3":"711.4","OPC-3uM-7":"573.3","OPC-10uM-4":"645.8","OPC-10uM-8":"652.4"},{"Metabolite":"Tyrosine","OPC-0uM-1":"5081","OPC-0uM-5":"5351","OPC-1uM-2":"4771","OPC-1uM-6":"5518","OPC-3uM-3":"5665","OPC-3uM-7":"5200","OPC-10uM-4":"5548","OPC-10uM-8":"5557"},{"Metabolite":"Valine","OPC-0uM-1":"8177","OPC-0uM-5":"8421","OPC-1uM-2":"7804","OPC-1uM-6":"8862","OPC-3uM-3":"9626","OPC-3uM-7":"8897","OPC-10uM-4":"9303","OPC-10uM-8":"9781"}],

"Metabolites":[{"Metabolite":"Adenine","KEGG ID":"C00147","HMDB ID":"HMDB00034"},{"Metabolite":"Adenosine","KEGG ID":"C00212","HMDB ID":"HMDB00050"},{"Metabolite":"Alanine","KEGG ID":"C00041,C00133,C01401","HMDB ID":"HMDB00161,HMDB01310"},{"Metabolite":"Arginine","KEGG ID":"C00062,C00792","HMDB ID":"HMDB00517,HMDB03416"},{"Metabolite":"Argininosuccinic acid","KEGG ID":"C03406","HMDB ID":"HMDB00052"},{"Metabolite":"Asparagine","KEGG ID":"C00152,C01905,C16438","HMDB ID":"HMDB00168"},{"Metabolite":"Aspartic acid","KEGG ID":"C00049,C00402,C16433","HMDB ID":"HMDB00191,HMDB06483"},{"Metabolite":"Beta-Alanine","KEGG ID":"C00099","HMDB ID":"HMDB00056"},{"Metabolite":"Betaine","KEGG ID":"C00719","HMDB ID":"HMDB00043"},{"Metabolite":"Betaine aldehyde","KEGG ID":"C00576","HMDB ID":"HMDB01252"},{"Metabolite":"Carnitine","KEGG ID":"C00318,C00487,C15025","HMDB ID":"HMDB00062"},{"Metabolite":"Carnosine","KEGG ID":"C00386","HMDB ID":"HMDB00033"},{"Metabolite":"Choline","KEGG ID":"C00114","HMDB ID":"HMDB00097"},{"Metabolite":"cis-3-hydroxyproline","KEGG ID":"C01015,C01157","HMDB ID":"HMDB06055,HMDB00725"},{"Metabolite":"Citrulline","KEGG ID":"C00327","HMDB ID":"HMDB00904"},{"Metabolite":"Creatine","KEGG ID":"C00300","HMDB ID":"HMDB00064"},{"Metabolite":"Creatinine","KEGG ID":"C00791","HMDB ID":"HMDB00562"},{"Metabolite":"Cystathionine","KEGG ID":"C00542,C02291","HMDB ID":"HMDB00099"},{"Metabolite":"Cysteine","KEGG ID":"C00097,C00736,C00793","HMDB ID":"HMDB00574,HMDB03417"},{"Metabolite":"Gamma-Aminobutyric acid","KEGG ID":"C00334","HMDB ID":"HMDB00112"},{"Metabolite":"Glutamic acid","KEGG ID":"C00025,C00217,C00302","HMDB ID":"HMDB00148,HMDB03339"},{"Metabolite":"Glutamine","KEGG ID":"C00064,C00303,C00819","HMDB ID":"HMDB00641,HMDB03423"},{"Metabolite":"Glutathione","KEGG ID":"C00051","HMDB ID":"HMDB00125"},{"Metabolite":"Glycine","KEGG ID":"C00037","HMDB ID":"HMDB00123"},{"Metabolite":"Guanosine","KEGG ID":"C00387","HMDB ID":"HMDB00133"},{"Metabolite":"Histidine","KEGG ID":"C00135,C00768,C06419","HMDB ID":"HMDB00177"},{"Metabolite":"Homocysteine","KEGG ID":"C00155,C05330","HMDB ID":"HMDB00742"},{"Metabolite":"Homoserine","KEGG ID":"C00263","HMDB ID":"HMDB00719"},{"Metabolite":"Inosine","KEGG ID":"C00294","HMDB ID":"HMDB00195"},{"Metabolite":"Isoleucine","KEGG ID":"C00407,C06418,C16434","HMDB ID":"HMDB00172"},{"Metabolite":"Leucine","KEGG ID":"C00123,C01570,C16439","HMDB ID":"HMDB00687"},{"Metabolite":"Lysine","KEGG ID":"C00047,C00739,C16440","HMDB ID":"HMDB00182,HMDB03405"},{"Metabolite":"Methionine","KEGG ID":"C00073,C00855,C01733","HMDB ID":"HMDB00696"},{"Metabolite":"Ornithine","KEGG ID":"C00077,C00515,C01602","HMDB ID":"HMDB00214,HMDB03374"},{"Metabolite":"Oxidized glutathione","KEGG ID":"C00127","HMDB ID":"HMDB03337"},{"Metabolite":"Phenylalanine","KEGG ID":"C00079,C02057,C02265","HMDB ID":"HMDB00159"},{"Metabolite":"Proline","KEGG ID":"C00148,C00763,C16435","HMDB ID":"HMDB00162,HMDB03411"},{"Metabolite":"Putrescine","KEGG ID":"C00134","HMDB ID":"HMDB01414"},{"Metabolite":"S-Adenosylhomocysteine","KEGG ID":"C00021","HMDB ID":"HMDB00939"},{"Metabolite":"S-Adenosylmethionine","KEGG ID":"C00019","HMDB ID":"HMDB01185"},{"Metabolite":"Sarcosine","KEGG ID":"C00213","HMDB ID":"HMDB00271"},{"Metabolite":"Serine","KEGG ID":"C00065,C00716,C00740","HMDB ID":"HMDB00187,HMDB03406"},{"Metabolite":"Spermidine","KEGG ID":"C00315","HMDB ID":"HMDB01257"},{"Metabolite":"Spermine","KEGG ID":"C00750","HMDB ID":"HMDB01256"},{"Metabolite":"Threonine","KEGG ID":"C00188,C00820","HMDB ID":"HMDB00167"},{"Metabolite":"Tryptophan","KEGG ID":"C00078,C00525,C00806","HMDB ID":"HMDB00929"},{"Metabolite":"Tyrosine","KEGG ID":"C00082,C01536,C06420","HMDB ID":"HMDB00158"},{"Metabolite":"Valine","KEGG ID":"C00183,C06417,C16436","HMDB ID":"HMDB00883"}]
}

}