{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001174","ANALYSIS_ID":"AN001949","VERSION":"1","CREATED_ON":"April 25, 2019, 3:56 pm"},

"PROJECT":{"PROJECT_TITLE":"The ClpCP complex modulates respiratory, but not fermentative, metabolism in Staphylococcus aureus and is regulated in a SrrAB-dependent manner.","PROJECT_SUMMARY":"The staphylococcal respiratory regulator (SrrAB) modulates energy metabolism in Staphylococcus aureus. Studies have suggested that regulated protein catabolism facilitates energy homeostasis. Regulated proteolysis in S. aureus is achieved through protein complexes composed of a peptidase (ClpQ or ClpP) in association with an AAA+ family ATPase (typically ClpC or ClpX). In the current report, we tested the hypothesis that SrrAB regulates a Clp complex to facilitate energy homeostasis in S. aureus. Strains deficient in one or more Clp complexes are attenuated for growth in the presence of puromycin, which causes enrichment of misfolded proteins. A ΔsrrAB strain had increased sensitivity to puromycin. Epistasis experiments suggested that the puromycin sensitivity phenotype of the ΔsrrAB strain was a result of decreased ClpC activity. Consistent with this, transcriptional activity of clpC was decreased in the ΔsrrAB mutant and overexpression of clpC suppressed the puromycin sensitivity of the ΔsrrAB strain. Genetic studies suggested that phosphorylated SrrA is required to influence puromycin resistance. ClpC positively influenced respiration in association with ClpP. ClpP was also required for optimal fermentative growth, whereas ClpC was dispensable. Metabolomics studies demonstrated that intracellular metabolic profiles of the ΔclpC and ΔsrrAB mutants are distinct from the wild type strain, supporting the notion that both ClpC and SrrAB affect central metabolism. We propose a model wherein SrrAB regulates energy homeostasis, in part, via modulation of regulated proteolysis.","INSTITUTE":"Montana State University","DEPARTMENT":"Chemistry and Biochemistry","LABORATORY":"Copie Lab","LAST_NAME":"Eilers","FIRST_NAME":"Brian","ADDRESS":"103 Chemistry and Biochemistry Bldg, RM 144, Valerie Copie Lab, Bozeman, Montana, 59717, USA","EMAIL":"brian.eilers@montana.edu","PHONE":"4069945116","PUBLICATIONS":"Journal of Bacteriology (accepted with revisions)"},

"STUDY":{"STUDY_TITLE":"Role of ClpCP in respiratory and fermentative growth","STUDY_SUMMARY":"To determine metabolite concentrations and differences at the 48 hour time point for WT, ClpC mutant, srrAB mutant, and ClpC:srrAB double mutant","INSTITUTE":"Montana State University","DEPARTMENT":"Chemistry and Biochemistry","LABORATORY":"Copie Lab","LAST_NAME":"Eilers","FIRST_NAME":"Brian","ADDRESS":"103 Chemistry and Biochemistry Bldg, RM 144, Valerie Copie Lab, Bozeman, Montana, 59717, USA","EMAIL":"brian.eilers@montana.edu","PHONE":"4069945116","NUM_GROUPS":"4 (WT, ClpC, srrAB, and ClpC:srrAB)","TOTAL_SUBJECTS":"4"},

"SUBJECT":{"SUBJECT_TYPE":"Bacteria","SUBJECT_SPECIES":"Staphylococcus aureus","TAXONOMY_ID":"1280"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"WT_1",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"WT_2",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"WT_3",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"WT_4",
"Factors":{"Genotype":"wild type"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC_1",
"Factors":{"Genotype":"ClpC deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC_2",
"Factors":{"Genotype":"ClpC deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC_3",
"Factors":{"Genotype":"ClpC deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC_4",
"Factors":{"Genotype":"ClpC deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"SrrAB_1",
"Factors":{"Genotype":"SrrAB deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"SrrAB_2",
"Factors":{"Genotype":"SrrAB deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"SrrAB_3",
"Factors":{"Genotype":"SrrAB deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"SrrAB_4",
"Factors":{"Genotype":"SrrAB deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC-SrrAB_1",
"Factors":{"Genotype":"ClpC-SrrAB double deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC-SrrAB_2",
"Factors":{"Genotype":"ClpC-SrrAB double deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC-SrrAB_3",
"Factors":{"Genotype":"ClpC-SrrAB double deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
},
{
"Subject ID":"-",
"Sample ID":"ClpC-SrrAB_4",
"Factors":{"Genotype":"ClpC-SrrAB double deletion mutant"},
"Additional sample data":{"Treatment":"48 hour growth"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Aerobic growth for all strains was performed on four biological replicates for each cell group. Overnight cultures diluted 1:1000 were used to inoculate 25 mL of fresh TSB in a 250 mL flask with 220 rpm agitation at 37° C. Aliquots of 10 mL were collected at 48 hrs , centrifuged at 5000 rpm for 5 minutes, then rinsed once with 1 mL of 1X PBS, and centrifuged at 5000 rpm for 5 minutes. The supernatant was discarded, and cell pellets were frozen at -80 °C until further use.","SAMPLE_TYPE":"Bacterial cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"deletion mutants were compared to the wild-type after 48 hours of growth"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Frozen cell pellets were resuspended in 1 mL of a 2:1 methanol/chloroform mixture and transferred to FastPrep lysis B matrix tubes (MP Biomedicals). Cells were lysed using the FastPrep-24 5G instrument and designated S. aureus settings (2 cycles at a speed of 6.0 m/s for 40 s)]. 300 μL of each layer of a 1:1 aqueous chloroform solution was added to each cell lysate. The tubes were vortexed, placed at -20 °C for 20 min, and centrifuged at 14,000 g for 10 minutes. 800 μL of the aqueous phase was transferred to microfuge tubes and placed in a Speedvac (no heat, manual run, volatile solvent) to dry overnight. Samples were resuspended in 600 μL of NMR buffer [0.25 mM 4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS), 0.4 mM imidazole, 25 mM phosphate buffer, 90% H2O/10% D2O] and transferred to 5 mm Bruker NMR tubes."},

"ANALYSIS":{"OPERATOR_NAME":"Brian Tripet"},

"NM":{"INSTRUMENT_NAME":"Bruker 600-MHz AVANCE III solution NMR spectrometer","INSTRUMENT_TYPE":"FT-NMR","NMR_EXPERIMENT_TYPE":"1D-1H","NMR_COMMENTS":"equipped with an automatic SampleJet sample loading system, as well as a 5 mm triple resonance (1H, 15N, 13C) liquid-helium-cooled TCI probe (cryoprobe) and Topspin software (Bruker version 3.2).","SPECTROMETER_FREQUENCY":"1H Larmor frequency","NMR_PROBE":"cryoprobe","NMR_SOLVENT":"10%D20","NMR_TUBE_SIZE":"5mm","SHIMMING_METHOD":"topshim","PULSE_SEQUENCE":"zgesgp","WATER_SUPPRESSION":"qfil"},

"NMR_METABOLITE_DATA":{
"Units":"Attomoles/CFU",

"Data":[{"Metabolite":"ADP","WT_1":"67.3","WT_2":"87.5","WT_3":"65.4","WT_4":"69.2","ClpC_1":"139.5","ClpC_2":"139.7","ClpC_3":"129.4","ClpC_4":"139.9","SrrAB_1":"127.4","SrrAB_2":"132","SrrAB_3":"134.8","SrrAB_4":"109.5","ClpC-SrrAB_1":"117.6","ClpC-SrrAB_2":"105.3","ClpC-SrrAB_3":"137.1","ClpC-SrrAB_4":"104.7"},{"Metabolite":"AMP","WT_1":"498.8","WT_2":"464","WT_3":"444.5","WT_4":"424","ClpC_1":"503.7","ClpC_2":"462.5","ClpC_3":"456.3","ClpC_4":"467.2","SrrAB_1":"504.3","SrrAB_2":"543.1","SrrAB_3":"640","SrrAB_4":"552.9","ClpC-SrrAB_1":"294","ClpC-SrrAB_2":"317.7","ClpC-SrrAB_3":"456.6","ClpC-SrrAB_4":"388.8"},{"Metabolite":"Acetate","WT_1":"1244.9","WT_2":"1317.9","WT_3":"1774.4","WT_4":"1605.9","ClpC_1":"2306.8","ClpC_2":"2406.2","ClpC_3":"2211.4","ClpC_4":"2455.2","SrrAB_1":"2033.5","SrrAB_2":"2135.1","SrrAB_3":"2376.3","SrrAB_4":"1614.2","ClpC-SrrAB_1":"2563.2","ClpC-SrrAB_2":"2707.2","ClpC-SrrAB_3":"2366.7","ClpC-SrrAB_4":"2269.8"},{"Metabolite":"Asparagine","WT_1":"10666.1","WT_2":"10999.3","WT_3":"10826.1","WT_4":"10963.5","ClpC_1":"13310.6","ClpC_2":"13739.8","ClpC_3":"12333.8","ClpC_4":"13152.9","SrrAB_1":"18197.8","SrrAB_2":"19128.3","SrrAB_3":"16820.9","SrrAB_4":"13053.5","ClpC-SrrAB_1":"12881","ClpC-SrrAB_2":"14657.4","ClpC-SrrAB_3":"17763.3","ClpC-SrrAB_4":"12881.1"},{"Metabolite":"Aspartate","WT_1":"5642.4","WT_2":"5511.8","WT_3":"3270.4","WT_4":"3160.2","ClpC_1":"4210","ClpC_2":"4880.7","ClpC_3":"3271.2","ClpC_4":"3117.2","SrrAB_1":"3785.7","SrrAB_2":"3785.9","SrrAB_3":"6748.9","SrrAB_4":"5683.7","ClpC-SrrAB_1":"4114.8","ClpC-SrrAB_2":"4666.2","ClpC-SrrAB_3":"7078.2","ClpC-SrrAB_4":"6468.6"},{"Metabolite":"Betaine","WT_1":"7077.9","WT_2":"7164.5","WT_3":"8023.8","WT_4":"7880.2","ClpC_1":"9240.6","ClpC_2":"9691.3","ClpC_3":"10984.5","ClpC_4":"10976.4","SrrAB_1":"5446.2","SrrAB_2":"4980.9","SrrAB_3":"10506.2","SrrAB_4":"9073.8","ClpC-SrrAB_1":"6321","ClpC-SrrAB_2":"7438.2","ClpC-SrrAB_3":"9760.5","ClpC-SrrAB_4":"9024.6"},{"Metabolite":"Citrulline","WT_1":"179.5","WT_2":"193.6","WT_3":"176.2","WT_4":"131.5","ClpC_1":"186.1","ClpC_2":"199.5","ClpC_3":"299.8","ClpC_4":"187.9","SrrAB_1":"208.6","SrrAB_2":"267.7","SrrAB_3":"230.8","SrrAB_4":"244.9","ClpC-SrrAB_1":"109.8","ClpC-SrrAB_2":"112.5","ClpC-SrrAB_3":"109.2","ClpC-SrrAB_4":"114.9"},{"Metabolite":"Dimethylamine","WT_1":"1598.1","WT_2":"1584","WT_3":"3024.2","WT_4":"2827.3","ClpC_1":"1285.1","ClpC_2":"1301.6","ClpC_3":"1598","ClpC_4":"1722.1","SrrAB_1":"4015.4","SrrAB_2":"4053.5","SrrAB_3":"3585.8","SrrAB_4":"2985.5","ClpC-SrrAB_1":"1598.7","ClpC-SrrAB_2":"1820.7","ClpC-SrrAB_3":"1169.1","ClpC-SrrAB_4":"1080.6"},{"Metabolite":"Formate","WT_1":"140.7","WT_2":"151.8","WT_3":"134.8","WT_4":"144.7","ClpC_1":"196.4","ClpC_2":"191.7","ClpC_3":"198.9","ClpC_4":"210.7","SrrAB_1":"152.6","SrrAB_2":"164.1","SrrAB_3":"198.8","SrrAB_4":"164","ClpC-SrrAB_1":"155.7","ClpC-SrrAB_2":"180.9","ClpC-SrrAB_3":"179.4","ClpC-SrrAB_4":"177.9"},{"Metabolite":"GTP","WT_1":"63.3","WT_2":"70.6","WT_3":"65.6","WT_4":"68.2","ClpC_1":"56.1","ClpC_2":"45.5","ClpC_3":"63.3","ClpC_4":"55.2","SrrAB_1":"98.1","SrrAB_2":"92.9","SrrAB_3":"98.2","SrrAB_4":"90.8","ClpC-SrrAB_1":"31.5","ClpC-SrrAB_2":"35.4","ClpC-SrrAB_3":"30.6","ClpC-SrrAB_4":"29.4"},{"Metabolite":"Glutamate","WT_1":"1635.3","WT_2":"1691.1","WT_3":"1713.9","WT_4":"1647.8","ClpC_1":"1847.4","ClpC_2":"1736.4","ClpC_3":"2182.8","ClpC_4":"1607.1","SrrAB_1":"823.3","SrrAB_2":"823.4","SrrAB_3":"1580.9","SrrAB_4":"1442.5","ClpC-SrrAB_1":"802.8","ClpC-SrrAB_2":"760.2","ClpC-SrrAB_3":"945","ClpC-SrrAB_4":"885"},{"Metabolite":"Glutarate","WT_1":"713.2","WT_2":"703.8","WT_3":"1621.9","WT_4":"1603.5","ClpC_1":"569.8","ClpC_2":"626.2","ClpC_3":"591","ClpC_4":"586","SrrAB_1":"3412.3","SrrAB_2":"3412.2","SrrAB_3":"2360.3","SrrAB_4":"1961.8","ClpC-SrrAB_1":"1092.6","ClpC-SrrAB_2":"1226.7","ClpC-SrrAB_3":"855","ClpC-SrrAB_4":"794.7"},{"Metabolite":"Glycine","WT_1":"69.9","WT_2":"78.4","WT_3":"49.9","WT_4":"43.3","ClpC_1":"149.8","ClpC_2":"149.9","ClpC_3":"150","ClpC_4":"162.1","SrrAB_1":"55.6","SrrAB_2":"55.7","SrrAB_3":"55.8","SrrAB_4":"68.9","ClpC-SrrAB_1":"105.2","ClpC-SrrAB_2":"105.3","ClpC-SrrAB_3":"133.8","ClpC-SrrAB_4":"132.6"},{"Metabolite":"Histamine","WT_1":"109.2","WT_2":"109.2","WT_3":"115.3","WT_4":"119.1","ClpC_1":"167.1","ClpC_2":"207.3","ClpC_3":"216.9","ClpC_4":"202.3","SrrAB_1":"95.6","SrrAB_2":"75.4","SrrAB_3":"95.7","SrrAB_4":"81.2","ClpC-SrrAB_1":"152.1","ClpC-SrrAB_2":"160.5","ClpC-SrrAB_3":"218.7","ClpC-SrrAB_4":"203.4"},{"Metabolite":"Histidine","WT_1":"90.4","WT_2":"93.4","WT_3":"113.6","WT_4":"108.2","ClpC_1":"63.3","ClpC_2":"86.6","ClpC_3":"141.5","ClpC_4":"128.1","SrrAB_1":"100.6","SrrAB_2":"113.2","SrrAB_3":"140.6","SrrAB_4":"120.6","ClpC-SrrAB_1":"124.2","ClpC-SrrAB_2":"144.6","ClpC-SrrAB_3":"143.1","ClpC-SrrAB_4":"136.2"},{"Metabolite":"Isoleucine","WT_1":"283.1","WT_2":"226.8","WT_3":"250.4","WT_4":"243.8","ClpC_1":"269.6","ClpC_2":"230.3","ClpC_3":"297.7","ClpC_4":"305.1","SrrAB_1":"357.8","SrrAB_2":"320","SrrAB_3":"330.5","SrrAB_4":"284.6","ClpC-SrrAB_1":"346.5","ClpC-SrrAB_2":"238.5","ClpC-SrrAB_3":"246.9","ClpC-SrrAB_4":"235.5"},{"Metabolite":"Lactate","WT_1":"187.2","WT_2":"187","WT_3":"169.4","WT_4":"156.2","ClpC_1":"247.8","ClpC_2":"231.6","ClpC_3":"229.4","ClpC_4":"231.9","SrrAB_1":"242.2","SrrAB_2":"256.3","SrrAB_3":"320.3","SrrAB_4":"249.5","ClpC-SrrAB_1":"153.6","ClpC-SrrAB_2":"208.2","ClpC-SrrAB_3":"246.3","ClpC-SrrAB_4":"219.6"},{"Metabolite":"Leucine","WT_1":"631.1","WT_2":"674.6","WT_3":"810.6","WT_4":"761.2","ClpC_1":"635.4","ClpC_2":"682.6","ClpC_3":"635.6","ClpC_4":"838.8","SrrAB_1":"1041.5","SrrAB_2":"989.2","SrrAB_3":"1100.6","SrrAB_4":"927.7","ClpC-SrrAB_1":"913.5","ClpC-SrrAB_2":"703.5","ClpC-SrrAB_3":"743.4","ClpC-SrrAB_4":"694.2"},{"Metabolite":"Lysine","WT_1":"10400.7","WT_2":"8755.8","WT_3":"10425.9","WT_4":"10263.3","ClpC_1":"13124.2","ClpC_2":"11183.4","ClpC_3":"13124.4","ClpC_4":"11431.8","SrrAB_1":"14144.9","SrrAB_2":"12574.5","SrrAB_3":"14728.6","SrrAB_4":"11981.2","ClpC-SrrAB_1":"11912.4","ClpC-SrrAB_2":"12479.1","ClpC-SrrAB_3":"12071.1","ClpC-SrrAB_4":"11647.8"},{"Metabolite":"Methionine","WT_1":"306.8","WT_2":"341","WT_3":"340.9","WT_4":"342.6","ClpC_1":"645.5","ClpC_2":"694.4","ClpC_3":"590.6","ClpC_4":"611.5","SrrAB_1":"402.2","SrrAB_2":"396","SrrAB_3":"561.5","SrrAB_4":"511.7","ClpC-SrrAB_1":"469.2","ClpC-SrrAB_2":"569.1","ClpC-SrrAB_3":"814.8","ClpC-SrrAB_4":"777.3"},{"Metabolite":"NAD+","WT_1":"316.9","WT_2":"350.1","WT_3":"373.4","WT_4":"347.5","ClpC_1":"610","ClpC_2":"702.5","ClpC_3":"741.2","ClpC_4":"640.5","SrrAB_1":"489.8","SrrAB_2":"480","SrrAB_3":"432.9","SrrAB_4":"424.3","ClpC-SrrAB_1":"521.1","ClpC-SrrAB_2":"618","ClpC-SrrAB_3":"744.9","ClpC-SrrAB_4":"722.4"},{"Metabolite":"NADP+","WT_1":"21.2","WT_2":"26.4","WT_3":"36.5","WT_4":"36.9","ClpC_1":"30.5","ClpC_2":"33.7","ClpC_3":"48","ClpC_4":"30.9","SrrAB_1":"30.8","SrrAB_2":"33.2","SrrAB_3":"32.6","SrrAB_4":"33.8","ClpC-SrrAB_1":"25.2","ClpC-SrrAB_2":"30.3","ClpC-SrrAB_3":"33.6","ClpC-SrrAB_4":"26.1"},{"Metabolite":"Niacinamide","WT_1":"25.9","WT_2":"25.8","WT_3":"23.8","WT_4":"26","ClpC_1":"39.6","ClpC_2":"55.5","ClpC_3":"35.8","ClpC_4":"44.9","SrrAB_1":"28.3","SrrAB_2":"43.1","SrrAB_3":"26.8","SrrAB_4":"28.3","ClpC-SrrAB_1":"27.6","ClpC-SrrAB_2":"37.8","ClpC-SrrAB_3":"52.5","ClpC-SrrAB_4":"40.8"},{"Metabolite":"Nicotinamide N-oxide","WT_1":"68.9","WT_2":"74.6","WT_3":"100","WT_4":"97.2","ClpC_1":"77.6","ClpC_2":"110.6","ClpC_3":"127.8","ClpC_4":"109.4","SrrAB_1":"169.5","SrrAB_2":"153.8","SrrAB_3":"101.8","SrrAB_4":"111.1","ClpC-SrrAB_1":"100.5","ClpC-SrrAB_2":"113.4","ClpC-SrrAB_3":"99","ClpC-SrrAB_4":"84.6"},{"Metabolite":"N-alpha-Acetyllysine","WT_1":"1437.9","WT_2":"1349.9","WT_3":"3033.9","WT_4":"2894.8","ClpC_1":"3469.1","ClpC_2":"3416.1","ClpC_3":"3899.2","ClpC_4":"4030.1","SrrAB_1":"3424","SrrAB_2":"3421.2","SrrAB_3":"1859.1","SrrAB_4":"1561.8","ClpC-SrrAB_1":"3491.7","ClpC-SrrAB_2":"3954","ClpC-SrrAB_3":"2452.8","ClpC-SrrAB_4":"2278.2"},{"Metabolite":"O-Phosphocholine","WT_1":"63.3","WT_2":"58.4","WT_3":"100","WT_4":"86.6","ClpC_1":"81","ClpC_2":"94.8","ClpC_3":"93.5","ClpC_4":"106.9","SrrAB_1":"125.8","SrrAB_2":"126.5","SrrAB_3":"133.2","SrrAB_4":"89.5","ClpC-SrrAB_1":"118.5","ClpC-SrrAB_2":"130.8","ClpC-SrrAB_3":"117.9","ClpC-SrrAB_4":"115.8"},{"Metabolite":"Phenylalanine","WT_1":"269.4","WT_2":"288.5","WT_3":"319.3","WT_4":"313.6","ClpC_1":"356.8","ClpC_2":"357","ClpC_3":"442.9","ClpC_4":"405.5","SrrAB_1":"404.1","SrrAB_2":"393.2","SrrAB_3":"404","SrrAB_4":"332.3","ClpC-SrrAB_1":"442.5","ClpC-SrrAB_2":"390","ClpC-SrrAB_3":"399.6","ClpC-SrrAB_4":"343.8"},{"Metabolite":"Succinate","WT_1":"238.1","WT_2":"246.4","WT_3":"222.8","WT_4":"214.8","ClpC_1":"225.7","ClpC_2":"238.1","ClpC_3":"199.8","ClpC_4":"199.5","SrrAB_1":"320.9","SrrAB_2":"318.2","SrrAB_3":"684","SrrAB_4":"610.5","ClpC-SrrAB_1":"184.8","ClpC-SrrAB_2":"239.1","ClpC-SrrAB_3":"368.7","ClpC-SrrAB_4":"346.8"},{"Metabolite":"Tartrate","WT_1":"244.2","WT_2":"244.3","WT_3":"244.6","WT_4":"244.7","ClpC_1":"280","ClpC_2":"281.1","ClpC_3":"281.4","ClpC_4":"281.5","SrrAB_1":"277.2","SrrAB_2":"277.3","SrrAB_3":"277.6","SrrAB_4":"277.5","ClpC-SrrAB_1":"239.3","ClpC-SrrAB_2":"239.4","ClpC-SrrAB_3":"239.5","ClpC-SrrAB_4":"239.8"},{"Metabolite":"Theophylline","WT_1":"15.8","WT_2":"9.2","WT_3":"14.6","WT_4":"12.7","ClpC_1":"98.5","ClpC_2":"98.6","ClpC_3":"98.8","ClpC_4":"98.9","SrrAB_1":"12","SrrAB_2":"15.4","SrrAB_3":"30.8","SrrAB_4":"12.9","ClpC-SrrAB_1":"91.1","ClpC-SrrAB_2":"91.2","ClpC-SrrAB_3":"91.3","ClpC-SrrAB_4":"91.4"},{"Metabolite":"Threonine","WT_1":"247.5","WT_2":"218.4","WT_3":"201.4","WT_4":"192","ClpC_1":"314.8","ClpC_2":"280.8","ClpC_3":"283.9","ClpC_4":"269.6","SrrAB_1":"300.7","SrrAB_2":"300.8","SrrAB_3":"382.2","SrrAB_4":"300.9","ClpC-SrrAB_1":"199.8","ClpC-SrrAB_2":"249.6","ClpC-SrrAB_3":"296.1","ClpC-SrrAB_4":"260.7"},{"Metabolite":"Trigonelline","WT_1":"137.6","WT_2":"147.3","WT_3":"71.3","WT_4":"71.5","ClpC_1":"196.1","ClpC_2":"235.9","ClpC_3":"203.2","ClpC_4":"179.5","SrrAB_1":"50.1","SrrAB_2":"50.2","SrrAB_3":"190.8","SrrAB_4":"169.8","ClpC-SrrAB_1":"117","ClpC-SrrAB_2":"136.2","ClpC-SrrAB_3":"206.1","ClpC-SrrAB_4":"195.6"},{"Metabolite":"Trimethylamine 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"Metabolites":[{"metabolite_name":"ADP"},{"metabolite_name":"AMP"},{"metabolite_name":"Acetate"},{"metabolite_name":"Asparagine"},{"metabolite_name":"Aspartate"},{"metabolite_name":"Betaine"},{"metabolite_name":"Citrulline"},{"metabolite_name":"Dimethylamine"},{"metabolite_name":"Formate"},{"metabolite_name":"GTP"},{"metabolite_name":"Glutamate"},{"metabolite_name":"Glutarate"},{"metabolite_name":"Glycine"},{"metabolite_name":"Histamine"},{"metabolite_name":"Histidine"},{"metabolite_name":"Isoleucine"},{"metabolite_name":"Lactate"},{"metabolite_name":"Leucine"},{"metabolite_name":"Lysine"},{"metabolite_name":"Methionine"},{"metabolite_name":"NAD+"},{"metabolite_name":"NADP+"},{"metabolite_name":"Niacinamide"},{"metabolite_name":"Nicotinamide N-oxide"},{"metabolite_name":"N-alpha-Acetyllysine"},{"metabolite_name":"O-Phosphocholine"},{"metabolite_name":"Phenylalanine"},{"metabolite_name":"Succinate"},{"metabolite_name":"Tartrate"},{"metabolite_name":"Theophylline"},{"metabolite_name":"Threonine"},{"metabolite_name":"Trigonelline"},{"metabolite_name":"Trimethylamine N-oxide"},{"metabolite_name":"Tryptophan"},{"metabolite_name":"Tyrosine"},{"metabolite_name":"UMP"},{"metabolite_name":"Valine"},{"metabolite_name":"sn-Glycero-3-phosphocholine"}]
}

}