{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001188","ANALYSIS_ID":"AN001980","VERSION":"1","CREATED_ON":"June 9, 2019, 6:00 pm"},

"PROJECT":{"PROJECT_TITLE":"Delayed death in P. falciparum","PROJECT_SUMMARY":"Metabolite profiling across the 1st and 2nd intraerythrocytic developmental lifecycles of the malaria parasite P. falciparum following induction of delayed death with indolmycin treatment","INSTITUTE":"University of Melbourne","LAST_NAME":"Cobbold","FIRST_NAME":"Simon","ADDRESS":"Bio21 Institute","EMAIL":"simon.cobbold@unimelb.edu.au","PHONE":"383442351"},

"STUDY":{"STUDY_TITLE":"P. falciparum infected erythrocytes","STUDY_SUMMARY":"Metabolite profiling across the 1st and 2nd intraerythrocytic developmental lifecycles of the malaria parasite P. falciparum following induction of delayed death with indolmycin treatment","INSTITUTE":"University of Melbourne","LAST_NAME":"Cobbold","FIRST_NAME":"Simon","ADDRESS":"Bio21 Institute, 30 Flemington Road","EMAIL":"simon.cobbold@unimelb.edu.au","PHONE":"383442351"},

"SUBJECT":{"SUBJECT_TYPE":"Cultured cells","SUBJECT_SPECIES":"Plasmodium falciparum","TAXONOMY_ID":"5833"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=1_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=1_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=1_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=1_1",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=1_2",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=1_3",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=1_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=1_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=1_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=1_1",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=1_2",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=1_3",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=1_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=1_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=1_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=1_1",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=1_3",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=1_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=1_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=1_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=1_1",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=1_2",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=1_3",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=1_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=1_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=1_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=1_1",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=1_2",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=1_3",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"1"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=2_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=2_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=2_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=2_1",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=2_2",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=2_3",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=2_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=2_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=2_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=2_1",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=2_2",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=2_3",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=2_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=2_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=2_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=2_1",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=2_2",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=2_3",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=2_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=2_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=2_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=2_1",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=2_2",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=2_3",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=2_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=2_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=2_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=2_1",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=2_2",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=2_3",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"2"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=3_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Indolmycin_n=3_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=3_1",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"30h_Control_n=3_2",
"Factors":{"Treatment":"Control","Time (hr)":"30"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=3_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=3_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Indolmycin_n=3_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=3_1",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=3_2",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"58h_Control_n=3_3",
"Factors":{"Treatment":"Control","Time (hr)":"58"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=3_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=3_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Indolmycin_n=3_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=3_1",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=3_2",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"68h_Control_n=3_3",
"Factors":{"Treatment":"Control","Time (hr)":"68"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=3_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=3_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Indolmycin_n=3_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=3_1",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=3_2",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"78h_Control_n=3_3",
"Factors":{"Treatment":"Control","Time (hr)":"78"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=3_1",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=3_2",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Indolmycin_n=3_3",
"Factors":{"Treatment":"Indolmycin","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=3_1",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=3_2",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"3"}
},
{
"Subject ID":"-",
"Sample ID":"88h_Control_n=3_3",
"Factors":{"Treatment":"Control","Time (hr)":"88"},
"Additional sample data":{"Biological Replicate":"3"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"1x108 Cells per technical replicate were aliquoted into an individual microcentrifuge tube, centrifuged, washed once with 1mL ice-cold PBS, centrifuged, and metabolites extracted with 150uL 80% acetonitrile","SAMPLE_TYPE":"Cultured cells"},

"TREATMENT":{"TREATMENT_SUMMARY":"Prior to metabolite extraction, P. falciparum-infected human erythrocyte cultures were treated/untreated with indolmycin"},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Following metabolite extraction with 80% acetontrile. Samples were centrifuged at top speed for 5 minutes and the supernatant collected. Metabolite samples were then stored at -80 until ready for LC-MS analysis"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"HILIC","INSTRUMENT_NAME":"Agilent 6550","COLUMN_NAME":"SeQuant ZIC-HILIC (150 x 4.6mm, 3.5um)","FLOW_GRADIENT":"80%","FLOW_RATE":"0.3 mL/minute","COLUMN_TEMPERATURE":"35","METHODS_FILENAME":"pHILIC 45 minute","SOLVENT_A":"20mM Ammonium carbonate","SOLVENT_B":"Acetonitrile","COLUMN_PRESSURE":"50-150 bar","INJECTION_TEMPERATURE":"4"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6550 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"NEGATIVE","MS_COMMENTS":"Full m/z scan range across 2-35 minutes. Extended dynamic range, 0-1700 m/z"},

"MS_METABOLITE_DATA":{
"Units":"ion count",

"Data":[{"Metabolite":"Succinic acid 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}

}