{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001193","ANALYSIS_ID":"AN001988","VERSION":"1","CREATED_ON":"06-05-2024"},
"PROJECT":{"PROJECT_TITLE":"MARBLES (Markers of Autism Risk in Babies: Learning Early Sign) Enriched Cohort Study: Internal Metabolomic Biomarker Exposome and Development Disorders (IMBEDD)","PROJECT_TYPE":"NIH/NIEHS 1U2CES026560-01","PROJECT_SUMMARY":"This project aims to identify internal biomarkers of drug, food and microbial exposures associated to Autism Spectrum Disorder (ASD) and neurodevelopmental outcomes in an enriched-risk cohort. Using targeted and untargeted internal exposome approaches to identify exposures in maternal blood and child cord blood, internal metabolomics biomarkers will be associated with related exposures and also associated with ASD.","INSTITUTE":"Emory University","DEPARTMENT":"School of Medicine","LABORATORY":"Clinical Biomarkers Laboratory","LAST_NAME":"Tran","FIRST_NAME":"ViLinh","ADDRESS":"NA","EMAIL":"vtran6@emory.edu","PHONE":"(404) 727 5027","FUNDING_SOURCE":"NIEHS ES026560","DOI":"http://dx.doi.org/10.21228/M8MX2J"},
"STUDY":{"STUDY_TITLE":"MARBLES (Markers of Autism Risk in Babies: Learning Early Sign) Enriched Cohort Study: Internal Metabolomic Biomarker Exposome and Development Disorders (IMBEDD)","STUDY_SUMMARY":"This project aims to identify internal biomarkers of drug, food and microbial exposures associated to Autism Spectrum Disorder (ASD) and neurodevelopmental outcomes in an enriched-risk cohort. Using targeted and untargeted internal exposome approaches to identify exposures in maternal blood and child cord blood, internal metabolomics biomarkers will be associated with related exposures and also associated with ASD.","INSTITUTE":"Emory University","DEPARTMENT":"School of Medicine","LABORATORY":"Clincal Biomarkers Laboratory","LAST_NAME":"Uppal","FIRST_NAME":"Karan","ADDRESS":"NA","EMAIL":"kuppal2@emory.edu","PHONE":"(404) 727 5027","SUBMIT_DATE":"2019-06-11"},
"SUBJECT":{"SUBJECT_TYPE":"Cord Blood","SUBJECT_SPECIES":"Homo sapiens","TAXONOMY_ID":"9606","AGE_OR_AGE_RANGE":"pediatric samples - 0 to 3 yrs"},
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},
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},
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},
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},
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},
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},
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},
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},
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"Factors":{"Plasma":"Cord blood"},
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},
{
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"Sample ID":"C-1GZ15-CB-00_1",
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"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190419_M380_037,VT_190419_M380_039,VT_190419_M380_041"}
},
{
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},
{
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},
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},
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"Sample ID":"C-1H6G1-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190418_M380_127,VT_190418_M380_129,VT_190418_M380_131"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H6H9-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190418_M380_055,VT_190418_M380_057,VT_190418_M380_059"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H6J4-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190415_M380_103,VT_190415_M380_105,VT_190415_M380_107"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H6T2-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190416_M380_091,VT_190416_M380_093,VT_190416_M380_095"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H6U0-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190419_M380_073,VT_190419_M380_075,VT_190419_M380_077"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H6W6-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190418_M380_031,VT_190418_M380_033,VT_190418_M380_035"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H726-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190415_M380_121,VT_190415_M380_123,VT_190415_M380_125"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H734-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190416_M380_157,VT_190416_M380_159,VT_190416_M380_161"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H783-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190418_M380_253,VT_190418_M380_255,VT_190418_M380_257"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7B1-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190415_M380_181,VT_190415_M380_183,VT_190415_M380_185"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7F2-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190415_M380_241,VT_190415_M380_243,VT_190415_M380_245"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7K1-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190419_M380_097,VT_190419_M380_099,VT_190419_M380_101"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7P0-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190418_M380_205,VT_190418_M380_207,VT_190418_M380_209"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7S3-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190416_M380_193,VT_190416_M380_195,VT_190416_M380_197"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7X2-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190419_M380_151,VT_190419_M380_153,VT_190419_M380_155"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H7Y0-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190416_M380_211,VT_190416_M380_213,VT_190416_M380_215"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H858-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190415_M380_259,VT_190415_M380_261,VT_190415_M380_263"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H890-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190419_M380_055,VT_190419_M380_057,VT_190419_M380_059"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H8E3-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190418_M380_241,VT_190418_M380_243,VT_190418_M380_245"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H8K0-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190416_M380_121,VT_190416_M380_123,VT_190416_M380_125"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H8L8-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190419_M380_031,VT_190419_M380_033,VT_190419_M380_035"}
},
{
"Subject ID":"Cord Blood Study Sample",
"Sample ID":"C-1H8R5-CB-00_1",
"Factors":{"Plasma":"Cord blood"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Cord blood","Raw files":"VT_190415_M380_193,VT_190415_M380_195,VT_190415_M380_197"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_1a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_013,VT_190415_M380_015,VT_190415_M380_017"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_1b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_019,VT_190415_M380_021,VT_190415_M380_023"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_1c_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_145,VT_190415_M380_147,VT_190415_M380_149"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_1d_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_151,VT_190415_M380_153,VT_190415_M380_155"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_1e_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_277,VT_190415_M380_279,VT_190415_M380_281"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_1f_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_283,VT_190415_M380_285,VT_190415_M380_287"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_2a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_007,VT_190416_M380_009,VT_190416_M380_011"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_2b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_013,VT_190416_M380_015,VT_190416_M380_017"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_2c_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_139,VT_190416_M380_141,VT_190416_M380_143"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_2d_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_145,VT_190416_M380_147,VT_190416_M380_149"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_2e_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_271,VT_190416_M380_273,VT_190416_M380_275"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_2f_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_277,VT_190416_M380_279,VT_190416_M380_281"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_3a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_007,VT_190418_M380_009,VT_190418_M380_011"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_3b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_013,VT_190418_M380_015,VT_190418_M380_017"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_3c_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_139,VT_190418_M380_141,VT_190418_M380_143"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_3d_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_145,VT_190418_M380_147,VT_190418_M380_149"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_3e_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_271,VT_190418_M380_273,VT_190418_M380_275"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_3f_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_277,VT_190418_M380_279,VT_190418_M380_281"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_4a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_007,VT_190419_M380_009,VT_190419_M380_011"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_4b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_013,VT_190419_M380_015,VT_190419_M380_017"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_4c_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_139,VT_190419_M380_141,VT_190419_M380_143"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_4d_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_145,VT_190419_M380_147,VT_190419_M380_149"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_4e_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_193,VT_190419_M380_195,VT_190419_M380_197"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"chearplasma_4f_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_199,VT_190419_M380_201,VT_190419_M380_203"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"NIST SRM 1950_1a",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_001,VT_190415_M380_003,VT_190415_M380_005"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"NIST SRM 1950_2b",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_211,VT_190419_M380_213,VT_190419_M380_215"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_1a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_007,VT_190415_M380_009,VT_190415_M380_011"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_1b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190415","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190415_M380_289,VT_190415_M380_291,VT_190415_M380_293"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_2a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"1","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_001,VT_190416_M380_003,VT_190416_M380_005"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_2b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190416","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190416_M380_283,VT_190416_M380_285,VT_190416_M380_287"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_3a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"2","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_001,VT_190418_M380_003,VT_190418_M380_005"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_3b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190418","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190418_M380_283,VT_190418_M380_285,VT_190418_M380_287"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_4a_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"3","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_001,VT_190419_M380_003,VT_190419_M380_005"}
},
{
"Subject ID":"Quality Control",
"Sample ID":"Q-Std Plasma pool_4b_1",
"Factors":{"Plasma":"Plasma"},
"Additional sample data":{"Date Analyzed":"190419","Batch":"4","CHEAR_Project_ID":"2016-1438","Sample.Matrix":"Plasma","Raw files":"VT_190419_M380_205,VT_190419_M380_207,VT_190419_M380_209"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Cord blood samples were collected from infant participants at birth. Cord blood collected in tubes containing EDTA were transferred to 1.8ml freezer tubes and stored in a -70 degrees C freezer. The samples were shipped on dry ice from the University of California Davis biorepository storage facility and subsequently to the metabolomics analysis lab at Emory University.","SAMPLE_TYPE":"Cord Blood","STORAGE_CONDITIONS":"Described in summary"},
"TREATMENT":{"TREATMENT_SUMMARY":"Samples were received frozen in aliquouts of <250uL. Prior to analysis, samples were thawed and prepared for HRM analysis using the standard protocols described in the Sample Preparation section."},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile (Sigma Aldrich) containing 2.5 microliters of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, urine was equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000 rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h).","SAMPLEPREP_PROTOCOL_ID":"HRM_SP_082016_01","SAMPLEPREP_PROTOCOL_FILENAME":"EmoryUniversity_HRM_SP_082016_01.pdf","SAMPLEPREP_PROTOCOL_COMMENTS":"Date effective: 30 July 2016","EXTRACTION_METHOD":"2:1 acetonitrile: sample followed by vortexing and centrifugation"},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"The HILIC column is operated parallel to reverse phase column for simultaneous analytical separation and column flushing through the use of a dual head HPLC pump equipped with 10-port and 6-port switching valves. During operation of HILIC separation method, the MS is operated in positive ion mode and 10 microliters of sample is injected onto the HILIC column while the reverse phase column is flushing with wash solution. Flow rate is maintained at 0.35 mL/min until 1.5 min, increased to 0.4 mL/min at 4 min and held for 1 min. Solvent A is 100% LC-MS grade water, solvent B is 100% LC-MS grade acetonitrile and solvent C is 2% formic acid (v/v) in LC-MS grade water. Initial mobile phase conditions are 22.5% A, 75% B, 2.5% C hold for 1.5 min, with linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min, resulting in a total analytical run time of 5 min. During the flushing phase (reverse phase analytical separation), the HILIC column is equilibrated with a wash solution of 77.5% A, 20% B, 2.5% C.","METHODS_ID":"2% formic acid in LC-MS grade water","METHODS_FILENAME":"20160920_posHILIC120kres5min_ESI_c18negwash.meth","CHROMATOGRAPHY_COMMENTS":"Triplicate injections for each chromatography mode","INSTRUMENT_NAME":"Thermo Dionex Ultimate 3000","COLUMN_NAME":"Waters XBridge BEH Amide XP HILIC (50 x 2.1mm x 2.5um) with Thermo Accucore HILIC guard","COLUMN_TEMPERATURE":"60C","SAMPLE_INJECTION":"10 uL","SOLVENT_A":"100% water","SOLVENT_B":"100% acetonitrile","ANALYTICAL_TIME":"5 min","SAMPLE_LOOP_SIZE":"15 uL","SAMPLE_SYRINGE_SIZE":"100 uL","CHROMATOGRAPHY_TYPE":"HILIC"},
"ANALYSIS":{"LABORATORY_NAME":"Clinical Biomarkers Laboratory","ANALYSIS_TYPE":"MS","ANALYSIS_PROTOCOL_FILE":"EmoryUniversity_HRM_QEHF-MS_092017_v1.pdf","ACQUISITION_DATE":"4/15/19-4/19/19","SOFTWARE_VERSION":"Xcalibur 3.0.63","OPERATOR_NAME":"ViLinh Tran","DETECTOR_TYPE":"Orbitrap","DATA_FORMAT":"Profile"},
"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive HF hybrid Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","MS_COMMENTS":"NA","ION_MODE":"POSITIVE","CAPILLARY_TEMPERATURE":"250C","COLLISION_GAS":"N2","DRY_GAS_FLOW":"45","DRY_GAS_TEMP":"150C","MASS_ACCURACY":"< 3ppm","SPRAY_VOLTAGE":"3500","ACTIVATION_PARAMETER":"5.00E+05","ACTIVATION_TIME":"118ms","INTERFACE_VOLTAGE":"S-Lens RF level= 55","SCANNING_RANGE":"85-1275","MS_RESULTS_FILE":"ST001193_AN001988_Results.txt UNITS:peak area Has m/z:Yes Has RT:Yes RT units:Seconds"},
"MS_METABOLITE_DATA":{
"Units":"Peak area",
"Data":[{"Metabolite":"12-Hydroxydodecanoic 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