{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001272","ANALYSIS_ID":"AN002112","VERSION":"1","CREATED_ON":"November 7, 2019, 1:57 pm"},
"PROJECT":{"PROJECT_TITLE":"Growth cone Membrane lipidomics and Growth cone particulate at different developmental stages in C57BL/6 mice","PROJECT_TYPE":"untargeted LC-MS/MS lipidomics","PROJECT_SUMMARY":"We performed high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP). These fractions were generated through established differential centrifugation techniques and lipid extraction. Developmental time points were chosen based upon our understanding of brain development in the murine model. In mice cortex, E18 is characterized by extension or elongation of axons toward targets, while around P0-P3 is when axons start innervating targets and branching occurs. At P6-P7 is when target innervation is generally complete and a subpopulation of cortical neurons undergo developmental cell death. By P9 growth cones have mostly disappeared.","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology, Bascom Palmer Eye Institute","LABORATORY":"Sanjoy K. Bhattacharya","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy","ADDRESS":"1638 NW 10th Avenue Miami, FL 33136","EMAIL":"SBhattacharya@med.miami.edu","PHONE":"305-482-4103","CONTRIBUTORS":"Jennifer Arcuri"},
"STUDY":{"STUDY_TITLE":"Growth cone memebrane and growth cone particulate lipidomics","STUDY_TYPE":"untargeted LC-MS/MS lipidomics","STUDY_SUMMARY":"We performed high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the proteome and lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP)","INSTITUTE":"University of Miami","DEPARTMENT":"Ophthalmology, Bascom Palmer Eye Institute","LABORATORY":"Sanjoy K. Bhattacharya","LAST_NAME":"Bhattacharya","FIRST_NAME":"Sanjoy","ADDRESS":"1638 NW 10th Avenue, Miami, FL 33136","EMAIL":"SBhattacharya@med.miami.edu","PHONE":"305-482-4103"},
"SUBJECT":{"SUBJECT_TYPE":"Mammal","SUBJECT_SPECIES":"Mus musculus","TAXONOMY_ID":"10090","GENOTYPE_STRAIN":"C57BL/6J","AGE_OR_AGE_RANGE":"E18-P90","WEIGHT_OR_WEIGHT_RANGE":"not measured","HEIGHT_OR_HEIGHT_RANGE":"not measured","GENDER":"Pooled","ANIMAL_ANIMAL_SUPPLIER":"The Jackson Laboratory","ANIMAL_HOUSING":"standard","ANIMAL_LIGHT_CYCLE":"standard 12h","ANIMAL_FEED":"standard grain-based","ANIMAL_WATER":"standard unlimited","ANIMAL_INCLUSION_CRITERIA":"random"},
"SUBJECT_SAMPLE_FACTORS":[
{
"Subject ID":"-",
"Sample ID":"GCP_E18_1+",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_1-",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_2+",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_2-",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_3+",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_3-",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_4+",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_4-",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_5+",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_5-",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_6+",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_E18_6-",
"Factors":{"Fraction":"GCP","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_1+",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_1-",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_2+",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_2-",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_3+",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_3-",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_4+",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_4-",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_5+",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_5-",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_6+",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P0_6-",
"Factors":{"Fraction":"GCP","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_1+",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_1-",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_2+",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_2-",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_3+",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_3-",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_4+",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_4-",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_5+",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_5-",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_6+",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P3_6-",
"Factors":{"Fraction":"GCP","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_1+",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_1-",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_2+",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_2-",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_3+",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_3-",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_4+",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_4-",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_5+",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_5-",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_6+",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P6_6-",
"Factors":{"Fraction":"GCP","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_1+",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_1-",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_2+",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_2-",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_3+",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_3-",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_4+",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_4-",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_5+",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_5-",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_6+",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCP_P9_6-",
"Factors":{"Fraction":"GCP","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_1+",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_1-",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_2+",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_2-",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_3+",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_3-",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_4+",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_4-",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_5+",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_5-",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_6+",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_E18_6-",
"Factors":{"Fraction":"GCM","Age":"E18"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_1+",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_1-",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_2+",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_2-",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_3+",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_3-",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_4+",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_4-",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_5+",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_5-",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_6+",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P0_6-",
"Factors":{"Fraction":"GCM","Age":"P0"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_1+",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_1-",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_2+.",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_2-",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_3+",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_3-",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_4+",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_4-",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_5+",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_5-",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_6+",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P3_6-",
"Factors":{"Fraction":"GCM","Age":"P3"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_1+",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_1-",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_2+",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_2-",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_3+",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_3-",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_4+",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_4-",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_5+",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_5-",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_6+",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P6_6-",
"Factors":{"Fraction":"GCM","Age":"P6"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_1+",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_1-",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_2+",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_2-",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_3+",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_3-",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_4+",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_4-",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_5+",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_5-",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_6+",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Positive"}
},
{
"Subject ID":"-",
"Sample ID":"GCM_P9_6-",
"Factors":{"Fraction":"GCM","Age":"P9"},
"Additional sample data":{"Ion Mode":"Negative"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Growth cone from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP) were collected. These fractions were generated through established differential centrifugation techniques and lipid extraction from established protocols: 1-Estrada-Bernal, A. et al. Functional complexity of the axonal growth cone: a proteomic analysis. PloS one 7, e31858 (2012). 2-Pfenninger et al 1983 paper (Pfenninger, K. H., Ellis, L., Johnson, M. P., Friedman, L. B. & Somlo, S. Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization. Cell 35, 573-584 (1983)","SAMPLE_TYPE":"Brain"},
"TREATMENT":{"TREATMENT_SUMMARY":"No Treatment"},
"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"We performed high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP)."},
"CHROMATOGRAPHY":{"CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Accela 600","COLUMN_NAME":"Thermo Acclaim C30 2.1x150 mm, 3µm"},
"ANALYSIS":{"ANALYSIS_TYPE":"MS"},
"MS":{"INSTRUMENT_NAME":"Thermo Q Exactive Orbitrap","INSTRUMENT_TYPE":"Orbitrap","MS_TYPE":"ESI","ION_MODE":"UNSPECIFIED","MS_COMMENTS":"Samples were ran in positive and negative ion mode"},
"MS_METABOLITE_DATA":{
"Units":"main area",
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}
}