{
"METABOLOMICS WORKBENCH":{"STUDY_ID":"ST001325","ANALYSIS_ID":"AN002206","VERSION":"1","CREATED_ON":"March 10, 2020, 1:46 pm"},

"PROJECT":{"PROJECT_TITLE":"Obesity and Poor Diet as Susceptibility Factors for Secondhand Smoke in Childhood Asthma","PROJECT_TYPE":"MS analysis","PROJECT_SUMMARY":"Investigating Overweight/Obesity and Poor Diet as Susceptibility Factors for Secondhand Smoke in Childhood Asthma","INSTITUTE":"Johns Hopkins University School of Medicine","DEPARTMENT":"Pulmonary & Critical Care Medicine","LABORATORY":"McCormack, Meredith Lab","LAST_NAME":"McCormack","FIRST_NAME":"Meredith","ADDRESS":"733 North Broadway, Suite G49 Baltimore, MD 21205-2196","EMAIL":"mmccor16@jhmi.edu","PHONE":"410-955-0036"},

"STUDY":{"STUDY_TITLE":"Obesity and Poor Diet as Susceptibility Factors for Secondhand Smoke in Childhood Asthma","STUDY_TYPE":"MS analysis","STUDY_SUMMARY":"Aim 1: To investigate the effect of SHS exposure on asthma morbidity, systemic inflammation and oxidative stress among inner-city children with asthma. Hypotheses 1.Increasing SHS exposure will be associated with increases in asthma morbidity, systemic inflammation and oxidative stress. Aim 2: To determine if being overweight/obese modifies the effect of SHS exposure on respiratory symptoms, inflammation and oxidative stress responses among inner-city children with asthma.Hypothesis 2. SHS exposure will be associated with a worsened asthma and increases in systemic inflammation and oxidative stress among overweight/obese children compared to normal weight children. Aim 3: To determine if diet quality modifies the effect of SHS exposure on respiratory symptoms, inflammation and oxidative stress responses among inner-city children with asthma. Hypothesis 3. SHS smoke exposure will associated with worsened respiratory symptoms and increases in inflammation and oxidative stress among children with poor quality diet compared those with better quality diet inner-city children with asthma. (Diet will be assessed by dietary inflammatory index, healthy eating index, and additional serum markers proposed in this application).","INSTITUTE":"University of Michigan","DEPARTMENT":"Biomedical Research Core Facilities","LABORATORY":"Metabolomics core","LAST_NAME":"Kachman","FIRST_NAME":"Maureen","ADDRESS":"Ann Arbor, MI","EMAIL":"mkachman@med.umich.edu","PHONE":"734-232-0842","TOTAL_SUBJECTS":"185","STUDY_COMMENTS":"Low-income minority children in urban areas often inhabit an environment that has excessive pollution and these children are at risk for obesity and poor diet. Secondhand smoke (SHS) is a common exposure in homes of children in Baltimore City and approximately 50-80% of households have a smoker. Secondhand smoke has been associated with worse asthma outcomes. We hypothesize that secondhand smoke exposure will be associated with increases in asthma morbidity and increases in systemic markers of inflammation and oxidative stress and that these responses will be exaggerated among overweight and obese children compared with normal weight and among those with poor diets compared to better diets among children with asthma."},

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},
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"Additional sample data":{"Batch":"2","Age":"9","CHEAR Sample name":"C-1QNQ5-S-07","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-S00041796-P.d 20190916-EX00973-A003-IN0029-S00041796-N.d"}
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},
{
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},
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{
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},
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{
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},
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{
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},
{
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},
{
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},
{
"Subject ID":"-",
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},
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},
{
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},
{
"Subject ID":"-",
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},
{
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},
{
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},
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},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"2","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-11-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-11-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"2","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-12-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-12-N.d"}
},
{
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},
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},
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"Subject ID":"-",
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},
{
"Subject ID":"-",
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},
{
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},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-06-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-06-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-07-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-07-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-08-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-08-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-09-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-09-N.d"}
},
{
"Subject ID":"-",
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},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-11-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-11-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-CS000QCMP-12-P.d 20190916-EX00973-A003-IN0029-CS000QCMP-12-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-1-01",
"Factors":{"Sample type":"chear plasma","Gender":"-"},
"Additional sample data":{"Batch":"1","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190909-EX00973-A003-IN0029-R00CHRPL1-01-P.d 20190909-EX00973-A003-IN0029-R00CHRPL1-01-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"1","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190909-EX00973-A003-IN0029-R00CHRPL1-02-P.d 20190909-EX00973-A003-IN0029-R00CHRPL1-02-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"1","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190909-EX00973-A003-IN0029-R00CHRPL1-03-P.d 20190909-EX00973-A003-IN0029-R00CHRPL1-03-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"1","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190909-EX00973-A003-IN0029-R00CHRPL1-04-P.d 20190909-EX00973-A003-IN0029-R00CHRPL1-04-N.d"}
},
{
"Subject ID":"-",
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"Additional sample data":{"Batch":"2","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-01-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-01-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-2-02",
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"Additional sample data":{"Batch":"2","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-02-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-02-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-2-03",
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"Additional sample data":{"Batch":"2","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-03-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-03-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-2-04",
"Factors":{"Sample type":"chear plasma","Gender":"-"},
"Additional sample data":{"Batch":"2","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-04-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-04-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-3-01",
"Factors":{"Sample type":"chear plasma","Gender":"-"},
"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-01-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-01-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-3-02",
"Factors":{"Sample type":"chear plasma","Gender":"-"},
"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-02-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-02-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-3-03",
"Factors":{"Sample type":"chear plasma","Gender":"-"},
"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-03-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-03-N.d"}
},
{
"Subject ID":"-",
"Sample ID":"R00CHRPL1-3-04",
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"Additional sample data":{"Batch":"3","Age":"-","CHEAR Sample name":"-","RAW_FILE_NAME":"20190916-EX00973-A003-IN0029-R00CHRPL1-04-P.d 20190916-EX00973-A003-IN0029-R00CHRPL1-04-N.d"}
}
],
"COLLECTION":{"COLLECTION_SUMMARY":"Standard blood serum collection","SAMPLE_TYPE":"Blood (serum)","STORAGE_CONDITIONS":"-80℃"},

"TREATMENT":{"TREATMENT_SUMMARY":"This is an observational study with no specific treatments applied."},

"SAMPLEPREP":{"SAMPLEPREP_SUMMARY":"Standard untargeted analysis sample preparation protocol","SAMPLEPREP_PROTOCOL_FILENAME":"Untargeted_metabolomics_analysis.doc"},

"CHROMATOGRAPHY":{"CHROMATOGRAPHY_SUMMARY":"Negative mode RP","CHROMATOGRAPHY_TYPE":"Reversed phase","INSTRUMENT_NAME":"Agilent 1290 Infinity II","COLUMN_NAME":"Waters Acquity BEH C8 (100 x 2.1mm, 1.7um)","METHODS_FILENAME":"Modified-Neg-LCMS1.m.7z"},

"ANALYSIS":{"ANALYSIS_TYPE":"MS"},

"MS":{"INSTRUMENT_NAME":"Agilent 6540 QTOF","INSTRUMENT_TYPE":"QTOF","MS_TYPE":"ESI","ION_MODE":"POSITIVE","MS_COMMENTS":"MS1 acquisition followed by targeted search and manual curation using Agilent ProFinder software"},

"MS_METABOLITE_DATA":{
"Units":"Peak area normalized",

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}

}